BRPI0700624A - processo de manipulação de sêmen - Google Patents

processo de manipulação de sêmen

Info

Publication number
BRPI0700624A
BRPI0700624A BRPI0700624-1A BRPI0700624A BRPI0700624A BR PI0700624 A BRPI0700624 A BR PI0700624A BR PI0700624 A BRPI0700624 A BR PI0700624A BR PI0700624 A BRPI0700624 A BR PI0700624A
Authority
BR
Brazil
Prior art keywords
sperm
semen
sperm cells
filtration
increase
Prior art date
Application number
BRPI0700624-1A
Other languages
English (en)
Inventor
Pedrazzi Cesar Augusto Ferraz
Original Assignee
Pedrazzi Cesar Augusto Ferraz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pedrazzi Cesar Augusto Ferraz filed Critical Pedrazzi Cesar Augusto Ferraz
Priority to BRPI0700624-1A priority Critical patent/BRPI0700624A/pt
Priority to US12/529,144 priority patent/US20100099075A1/en
Priority to PCT/BR2008/000018 priority patent/WO2008104042A1/en
Publication of BRPI0700624A publication Critical patent/BRPI0700624A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PROCESSO DE MANIPULAçãO DE SEMEN. A presente invenção refere-se a um processo de manipulação de sêmen visando a separação de células espermáticas de seu meio mantenedor. O processo de que trata a invenção proporciona um aumento na concentração final de espermatozóides obtidos e aumento nos índices de produção de embriões ín vitro apresentando uma grande aplicação na área de reprodução assistida de seres humanos e animais em geral. A invenção descreve um sistema de filtragem para separação de células espermáticas de seu meio mantenedor caracterizado por ser constituído por uma membrana filtrante que permanece em contato com uma placa primária colocada sobre uma placa aquecedora e que mantém a temperatura constante durante todo o processo. O procedimento inclui a filtração utilizando soluções de lavagem e soluções finais de manutenção que são selecionadas de acordo com a aplicação posterior das células espermáticas separadas. Os espermatozóides retidos na membrana de filtração são ressuspendidos podendo, os mesmos, serem utilizados para inseminação artificial, congelamento, fecundação in vitro ou sexagem dos espermatozóides X e Y no citómetro de fluxo.
BRPI0700624-1A 2007-02-28 2007-02-28 processo de manipulação de sêmen BRPI0700624A (pt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BRPI0700624-1A BRPI0700624A (pt) 2007-02-28 2007-02-28 processo de manipulação de sêmen
US12/529,144 US20100099075A1 (en) 2007-02-28 2008-01-22 Semen manipulation process
PCT/BR2008/000018 WO2008104042A1 (en) 2007-02-28 2008-01-22 Semen manipulation process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0700624-1A BRPI0700624A (pt) 2007-02-28 2007-02-28 processo de manipulação de sêmen

Publications (1)

Publication Number Publication Date
BRPI0700624A true BRPI0700624A (pt) 2008-10-14

Family

ID=39596825

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0700624-1A BRPI0700624A (pt) 2007-02-28 2007-02-28 processo de manipulação de sêmen

Country Status (3)

Country Link
US (1) US20100099075A1 (pt)
BR (1) BRPI0700624A (pt)
WO (1) WO2008104042A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2946885B1 (fr) * 2009-06-17 2011-08-26 Phagexel Composition de nacre destinee a corriger des defauts de nidation chez les mammiferes.
US10415031B2 (en) 2015-02-04 2019-09-17 InnoGenomics Technologies, LLC Method, apparatus and kit for human identification using polymer filter means for separation of sperm cells from biological samples that include other cell types
CA2995815C (en) * 2015-06-22 2021-08-24 The Brigham And Women's Hospital, Inc Home evaluation of the quality of semen samples
AR107746A1 (es) 2017-02-24 2018-05-30 Herberto Ernesto Hector Repetto Dispositivo y método de separación de células móviles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2614899B1 (fr) * 1987-05-07 1989-08-11 Ranoux Claude Dispositif pour preparation selection et capacitation des spermatozoides humains et animaux par filtration
US5185246A (en) * 1989-04-17 1993-02-09 Alice Deutsch Method for semen analysis employing membrane separation
AU3146800A (en) * 1999-03-05 2000-09-28 De Danske Kvaegavlsforeninger Determination of sperm concentration and viability for artificial insemination
IL131826A0 (en) * 1999-09-09 2001-03-19 Tennenbaum Tamar A method and device for artificial insemination
BRPI0408857B1 (pt) * 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
US20050032097A1 (en) * 2003-06-17 2005-02-10 Garvin Alex M. Method for processing samples containing sperm and non-sperm cells for subsequent analysis of the sperm DNA

Also Published As

Publication number Publication date
WO2008104042A1 (en) 2008-09-04
US20100099075A1 (en) 2010-04-22

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Legal Events

Date Code Title Description
B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08G Application fees: restoration [chapter 8.7 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements