BRPI0705683A - método para produzir flores macho estéreis e frutos partenocárpicos mediante silenciamento gênico, seqüências associadas e vetores que as contêm - Google Patents

método para produzir flores macho estéreis e frutos partenocárpicos mediante silenciamento gênico, seqüências associadas e vetores que as contêm

Info

Publication number
BRPI0705683A
BRPI0705683A BRPI0705683-4A BRPI0705683A BRPI0705683A BR PI0705683 A BRPI0705683 A BR PI0705683A BR PI0705683 A BRPI0705683 A BR PI0705683A BR PI0705683 A BRPI0705683 A BR PI0705683A
Authority
BR
Brazil
Prior art keywords
fruits
male flowers
genes
parthenocarpal
sterile male
Prior art date
Application number
BRPI0705683-4A
Other languages
English (en)
Inventor
Jorge Patricio Arce Johnson
Maria Josefina Poupin Swinburn
Maria Consuelo Medina Arevalo
Agnes Cadavid Labrada
Fernan Federici Noe
Original Assignee
Univ Pontificia Catolica Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pontificia Catolica Chile filed Critical Univ Pontificia Catolica Chile
Publication of BRPI0705683A publication Critical patent/BRPI0705683A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

MéTODO PARA PRODUZIR FLORES MACHO ESTéREIS E FRUTOS PARTENOCáRPICOS MEDIANTE SILENCIAMENTO GêNICO, SEQüENCIAS ASSOCIADAS E VETORES QUE AS CONTêM Método para produzir flores macho estéreis e frutos partenocárpicos por meio de silenciamento gênico que consta das seguintes etapas: a) obter a seqüência codificadora de genes homólogos para Pistillata (PI) da espécie desejada; b) analisar a expressão da seqüência obtida para verificar se é expressa de acordo com o padrão descrito para genes Pistillata; c) analisar a complementação de mutantes para o gene PI com a seqüência PI obtida da espécie desejada para comprovar que a seqüência obtida cumpre a função do gene PI; d) fabricar a construção para silenciamento gênico que contenha uma zona da seqüência codificadora de PI em um vetor de expressão em plantas; e) incorporação do vetor construído em Agrobacterium tumefaciens; f) transformar as plantas de interesse com Agrobacterium tumeifaciens modificado pelo vetor de silenciamento e seleção das plantas transformadas; g) comprovar a ausência de contaminação por Agrobacterium e corroboração das plantas transgênicas por amplificação do transgene. São revelados, ainda, os genes VvPI de Vitis vinifera variedade Cabernet sauvignon e LePI de Lycopersicon esculentum; e o uso desses genes para produzir flores macho estéreis e frutos sem sementes ou partenocárpicos. Conjuntamente, são revelados vetores de silenciamento que contêm essas seqüências ou parte delas.
BRPI0705683-4A 2006-12-01 2007-11-29 método para produzir flores macho estéreis e frutos partenocárpicos mediante silenciamento gênico, seqüências associadas e vetores que as contêm BRPI0705683A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2006003361 2006-12-01

Publications (1)

Publication Number Publication Date
BRPI0705683A true BRPI0705683A (pt) 2008-07-15

Family

ID=42060733

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0705683-4A BRPI0705683A (pt) 2006-12-01 2007-11-29 método para produzir flores macho estéreis e frutos partenocárpicos mediante silenciamento gênico, seqüências associadas e vetores que as contêm

Country Status (6)

Country Link
US (1) US7994397B2 (pt)
CN (1) CN101275139B (pt)
AR (1) AR069998A1 (pt)
AU (1) AU2007237251B2 (pt)
BR (1) BRPI0705683A (pt)
CA (1) CA2615249C (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102965373B (zh) * 2012-11-09 2014-03-26 中国农业大学 一种dna片段及其在制备雄性不育辣椒中的应用
CN107109408A (zh) * 2014-08-01 2017-08-29 新西兰植物和食品研究院有限公司 生产无核果实的方法和材料
DE102016106656A1 (de) * 2016-04-12 2017-10-12 Kws Saat Se Kernkodierte männliche Sterilität durch Mutation in Cytochrom P450 Oxidase
PL3464333T3 (pl) * 2016-05-26 2024-09-30 Nunhems B.V. Rośliny wytwarzające bezpestkowe owoce
CN113106119B (zh) * 2021-04-16 2022-09-27 山东大丰园农业有限公司 一种抑制蓝莓果实内源基因表达的方法、系统与应用
CN114410661A (zh) * 2021-12-29 2022-04-29 河南农业大学 一种天目地黄的遗传转化方法
CN117384914B (zh) * 2023-07-11 2024-11-01 云南大学 MhEML4基因克隆及其在种子败育中的应用
WO2025185745A1 (zh) * 2024-03-08 2025-09-12 中国科学院遗传与发育生物学研究所 自动化杂交育种
CN118480576B (zh) * 2024-06-04 2025-05-09 海南师范大学 芝麻ap3基因在调控雄蕊数量中的应用及芝麻vigs体系的建立方法
CN118995753B (zh) * 2024-10-15 2025-05-06 中国农业大学 一种沉默抑制因子RhHDP2及其应用
CN120060336B (zh) * 2025-02-28 2026-04-03 广州大学 大豆PISTILLATAd（PId）基因及其敲除方法与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1004722C2 (nl) 1996-12-06 1998-06-09 Western Seed Espana S A Zaadloze tomaat en werkwijze voor het verkrijgen hiervan, hybride tomatenplanten die deze kunnen dragen en kweekmateriaal hiervoor, alsmede uit deze tomaten verkregen voedselprodukten.
US6483012B1 (en) 1996-12-20 2002-11-19 Max-Planck-Gesellschaft zur Förederung der Wissenschaften e.V. Methods for producing parthenocarpic or female sterile transgenic plants and methods for enhancing fruit setting and development
US20020152495A1 (en) 1999-01-15 2002-10-17 Toshiro Ito Plants having seedless fruit

Also Published As

Publication number Publication date
CN101275139B (zh) 2014-02-05
AR069998A1 (es) 2010-03-10
AU2007237251A1 (en) 2008-06-19
CN101275139A (zh) 2008-10-01
AU2007237251B2 (en) 2013-06-27
US7994397B2 (en) 2011-08-09
US20080216194A1 (en) 2008-09-04
CA2615249C (en) 2014-02-18
CA2615249A1 (en) 2008-06-01

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Legal Events

Date Code Title Description
B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 9A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]