BRPI0708951A2 - fatigue resistant plants - Google Patents

Abstract

PLANTS RESISTANT TO FATIGUE. The present invention relates to a fatigue tolerance in plants and plant cells that is obtained by using nucleotide sequences that encode enzymes involved in the NAD recovery synthesis pathway and / or the NAD de novo synthesis pathway. , from fungi or yeast-like organisms, different from Saccharomyces cerevisae, for example, for overexpression in plants.

Description

Descriptive Report of the Invention Patent for "FATIGUE RESISTANT PLANTS".

The present invention relates to methods for increasing resistance to plant fatigue and plant cells, wherein enzymes involved in the pathway of NAD recovery synthesis and / or the pathway of new NAD synthesis originating from organisms or Fungal yeasts, unlike Saccharomyces cerevisae, are expressed in plants.

Tolerance of plants to adverse growing conditions, including dry weather, high light intensities, high temperatures, nutrient limitations, saline and other growing conditions, is a much desired property for plant crops, as never before. research is finished on how the actual production of these plants will finally increase.

Several ways of achieving the goal of improving what is commonly known as fatigue strength or fatigue tolerance have been described. Since different abiotic conditions of fatigue often result in the generation of harmful oxygen-reactive species ("ROS") such as hydrogen superoxides or peroxides, initial attempts to improve plant fatigue resistance have been focused on preventing the generation of ROS or removal thereof. Examples of such approaches include overexpression of ROS sequestering enzymes such as catalases, peroxidases, superoxide dismutases, etc., or even increasing the amount of ROS sequestering molecules, such as ascorbic acid, glutathione, etc. These approaches and other attempts to construct fatigue tolerant plants are reexamined, for example, by Wang et al. (2003), Planta, 218: 1-14.

Fatigue tolerance in plant and plant cells can also be achieved by reducing the activity or level of endogenous poly-ADP-ribose polymerases (ParP) or poly (ADP-ribose) glycohydrolases (ParG) as described in the patent document. WO 00/04173 and PCT / EP 2004/003995, respectively. Thus, it is thought that the properties of fatal NAD and ATP depletion in plant cells subjected to conditions of fatigue resulting in traumatic cell death can be avoided or sufficiently postponed for fatigue cells to survive and acclimate to fatigue conditions.

Uchimiya et al. (2002) describe that the isolation of a YK1-indicated rice umgene, as well as the use of the YK1 chimeric gene to increase tolerance of transgenic rice plants, protects talgene from rice blast and various abiotic fatigue such as , NaCI1UV-C, flood, and hydrogen peroxide (Uchimiya et al. (2002), Molecular Breeding, 9: 25-31).

Uchimiya and others have published an article describing that overexpression of a reductase-dependent NAD (YK1) gene in rice cells also promoted NAD (P) (H) level by over-regulating NAD synthetase activities and concluded whereas this modification, in turn, generated a cluster of redox substances necessary for fatigue strength of ROS (Uchimiya et al., (2003), Keystone Symposium on Plant Biology: Functions and Control of Cell Death, Snowbird, Utah, April 10-15). , 2003).

Yeast NAD synthetase has been well characterized, being the last enzyme in the pathway of de novo NAD synthesis and in the pathway of NAD recovery synthesis. On the new pathway, quinolate is the precursor for NAD synthesis, being generated as a product of tryptophan decomposition. In the pathway of NAD recovery, nicotinamide (which is a product of NAD decomposition, generated by the action of various enzymes such as PARP, NAD-dependent deacetylase, or other NAD glycohydrolases) is the precursor molecule. . In a first step, nicotinamide is deaminated to nicotinic acid by means of a nicotinamidase. Nicotinic acid is transferred to 5-phosphoribosyl-1-pyrophosphate by the phosphorylbosyl transferase enzyme mannicotinate to produce nicotinic acid mononucleotide. This compound is shared between the new and recovering paths. Thus, the subsequent conversion of this compound by NAD + pyrophosphorylase and NAD synthetase is obtained as in the new path. In yeast, overexpression of PNC1 (nicotinamidase coder) was correlated with the length of life by restriction. of low intensity calorie and fatigue (Anderson et al. (2003), Nature, 423; pages 181-185; Gallo et al. (2004), Molecular and CellularBiology, 24: 1301-1312).

WO 2004/016726 describes methods and compositions for modulating the lifespan of eukaryotic and procaryotic cells and for protecting cells against certain stresses. One method comprises modulating the NAD + recovery pathway flux in the cell, for example by modulating the activity level of one or more proteins selected from the group consisting of PNC1, NMA1, NPT1 and NMA2.

Little is known about the respective enzymes of NAD biosynthesis pathways in plants. Hunt et al. (2004) describe the use of available Arabidopsis genomic information to identify the plant homologues of these enzymes (Hunt et al. (2004), New Phytologist163 (1): 31-44). The identified DNA sequences have the following accession numbers: for nicotinamidase: At5g23220; At5g23230 and At3g16190; for nicotinate phosphoribosyltransferase: At4g36940, At2g23420, for adenylyltransferase nicotinic acid mononucleotide: At5g55810e for NAD synthetase: At1g55090 (all nucleotide sequences are incorporated herein by reference).

PCT / EP 2005/010168 describes methods for increasing fatigue strength in plants and plant cells, wherein the enzymes involved in the pathway of NAD recovery synthesis and / or the pathway of de novo synthesis of NAD, are expressed on the plants.

Alternative methods for increasing fatigue tolerance are still needed and the following embodiments, including the claims, provide such methods and means.

Summary of the Invention

In one embodiment of the invention, there is provided a method for obtaining a plant with increased fatigue strength comprising introducing a chimeric gene into a cell of a plant to obtain transgenic cells, wherein the chimeric gene comprises of the following operably linked DNA fragments:

(i) a passive plant expression promoter;

(ii) a DNA region encoding a functional enzyme depletes the nicotine mida adenine dinucleotide recovery synthesis pathway selected from nicotinamidase, nicotinate phosphorylbosyltransferase, nicotinic adenyl transferase mononucleotide or nicotinamide dinucleotide adenine synthetase;

(iii) a 3 'terminal region involved in transcription, termination and polyadenylation,

followed by the regeneration of transgenic cells to obtain a population of transgenic plants; and selecting a plant from the transgenic plant population that exhibits increased resistance to fatigue or selecting a plant that exhibits a low level of oxygen-reactive species or maintains a high level of NADH under faecal conditions. , when compared to a similar non-transgenic plant, in which method is characterized by the fact that the functional amino acid sequence of the plant encoded by the DNA region comprises one of the following: the amino acid sequence of accession number XP_444840 (Candida glabrata ), the amino acid sequence XP_456073 (Kluyveromyces lactis), the amino acid sequence NP_986013 (Eremothecium gossypii), the amino acid sequence XP_888958 (Candida albicans), the amino acid sequence XP500320 (Yarrowia Iipolyti-ca), the amino acid sequence of accession number XP389372 (Giberellazeae), the following Accession number amino acid sequence XP_749509 (As-pergillus fumigatus), Accession number amino acid sequenceXP_712112 (Candida albicans), Accession number amino acid sequence BAE56421 (Aspergillus oryzae), Accession number amino acid sequence access XP_567125 (Cryptococcus neofomans), accession a-mino sequence XP_964547 (Neurospora crassa), accession amino acid sequence XP_712135 (Candida albi-cans), accession amino acid sequence XP_448179 (Can-dida glabrata), accession number amino acid sequence XP_453643 (Kluyveromyces lactis), accession number amino acid sequence NP_987024 (Eremothecium gossypii), accession number amino acid sequence XP_500272 (Yarrowia lipolytica), amino acid sequence of accession number XP_722371 (Candida albicans), amino acid sequence of accession number XP_456405 (Debaromyceshansenii), accession number amino acid sequence BAE61562 (Aspergillus oryzae), accession number amino acid sequenceXP_759702 (Ustilago maydis), accession number amino acid sequence EAL18079 (Cryptococcus neoformans), accession number amino acid sequence (NP_587771ces pizombeomyces) ), accession amino acid sequence XP_681472 (Aspergillus nidu-lans), accession amino acid sequence XP_959191 (Neu-rospora crassa), accession amino acid sequenceXP_567726 (Cryptococcus neoformans), accession amino acid sequence EAQ90706 (Chaetomium globosum), accession amino acid sequence XP_387574 (Giberella zeae), accession amino acid sequence XP_748008 (Aspergillus fumigatus), accession amino acid sequence (XP_361704) Magnaporthegrisea), the amino acid sequence of accession number Q06178, the amino acid sequence accession number XP_444815 (Candida glabra-ta), accession number amino acid sequence NP_986687 ((Eremo-thecium gossypii), accession number amino acid sequenceXP_453005 (Kluyveromyces lactis), accession number amino acid sequence XP_458184 (Debaromyces hansenii), accession amino acid sequence XP_718656 (Candida albicans), accession amino acid sequence XP_504391 (Yarrowia lipolytica), NP_592856 accession amino acid sequence (Schizcesaccha-rom pombe), the accession number amino acid sequence XP_762639 (Ustilago maydis), the accession number amino acid sequence XP_571297 (Cryptococcus neoformans), the accession number amino acid sequence BAE57070 (Aspergillus oryzae), the number amino acid sequence XP_750776 (Aspergillus fumigatus), amino acid sequence of access number XP_659349 (Aspergillusnidulans), the sequence accession amino acid sequence XP_389652 (Giberella zeae), accession amino acid sequenceXP_957634 (Neurospora crassa), accession amino acid sequence XP_363364 (Magnaporthe grisea), accession amino acid sequence XP_75817is (Ustil ), the amino acid sequence of accession number EAQ85219 ((Chaetomium globosum), the amino acid sequence of accession number CAA85352 (Saccharomycescerevisae), the amino acid sequence of accession number XP_448893 (Candida glabrata), the sequence accession amino acid sequence XP_453357 (Kluyveromyces lactis), accession number amino acid sequence NP_983562 (Eremothecium gossypii), accession number amino acid sequence XP_462577 (Debaromyces hansenii), accession number amino acid sequence (Candida XP_888) albicans), the amino acid sequence of accession number XP_500338 (Yarrowia Iipoly-tica), the sequence of accession amino acids XP_746744 (As-pergillus fumigatus), accession amino acid sequence BA-E64333 (Aspergillus oryzae), accession amino acid sequence XP_965789 (Neurospora crassa), accession amino acid sequence EAQ93453 (Chaetomium globosum), accession amino acid sequence XP_682385 (Aspergillus nidulans), accession amino acid sequence AAN74808 (Gibberella monili-formis), accession amino acid sequence Q9UTK3, accession amino acid sequence XP_361075 (Magnaporthegrisea), accession amino acid sequence EAL18922 (Cryp-tococcus neoformans), accession amino acid sequenceXP_568039 (Cryptococcus neoformans), accession amino acid sequence XP_760597 (Ustilago maydis), the amino acid sequence of accession number NP_011524, the amino acid sequence of number of aces so XP_444815 (Candida glabrata), the accession amino acid sequence NP_986687 ((Eremothecium gossypii), the accession amino acid sequence XP_453005 (Kluyveromyces Iac-tis), the accession amino acid sequence XP_458184 (Deba-romyces hansenii), accession number amino acid sequenceXP_718656 (Candida albicans), accession number amino acid sequence XP_504391 (Yarrowia lipolytica), accession number amino acid sequence NP_592856 (Schizosaccharomyces pombe sequence) number XP_762639 (Ustilago maydis), the amino acid sequence XP_571297 (Cryptococcusneoformans), the amino acid sequence BAE57070 (Aspergillus oryzae), the amino acid sequenceXP_75077us (Aspergillum fumum) ), the amino acid sequence number XP_659349 (Aspergillus nidulans), the amino acid sequence number access code XP_389652 (Giberella zeae), accession number amino acid sequence XP_957634 (Neurospora crassa), accession number amino acid sequence XP_363364 (Magnaporthe grisea), accession number amino acid sequence XP_758179 (Ustilago may- dis) or the amino acid sequence of accession number EAQ85219 (Cha-etomium globosum),

In another embodiment, the invention relates to chimeric genes as described herein, to plant cells comprising such genesquimeric and to plants consisting essentially of plant cells comprising such chimeric genes, and even seeds of such plants. These plants and plant cells may be characterized by the fact that they have a lower level of oxygen-reactive species under fatigue conditions than a similar plant that does not comprise such a gene-polymer.

In yet another embodiment, the invention relates to the use of the described chimeric genes to increase the fatigue strength of a plant or to decrease the level of oxygen reactive species in a plant or plant cell under conditions of fatigue.

The invention further provides for the use of said DNA sequence encoding a plant functional enzyme of the nicotinamide adenine dinucleotide recovery pathway selected from denicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid adenyl transferase mononucleotide or nicotinamide adenine dinase nucleotide, of fungal origin or yeast type, to increase the resistance to fatigue of a plant or to decrease the level of oxygen reactive species or to maintain the level of NADH in a plant or plant cell under fatigue conditions.

Detailed Description of the Invention

The present invention is based on the discovery that DNA sequences encoding plant functional enzymes of the NAD recovery pathway in yeast can be used to obtain transgenic plants which were more resistant to fatigue, particularly abiotic fatigue, than plants that do not contain these DNA sequences. Transgenic plants also exhibited a significantly reduced level of oxygen reactive species ("ROS") and maintained a high level of NADH when placed under fatigue conditions when compared to control plants.

Thus, in one embodiment of the invention, there is provided a method for obtaining a plant with increased fatigue strength, wherein the method comprises the steps of:

introducing a fatigue resistant chimeric gene as described above into cells of a plant to obtain cells comprising the fatigue resistant chimeric gene;

- regenerating such cells comprising the fatigue-resistant chimeric gene to obtain a plant population comprising the fatigue resistant chimeric gene; and

- Selecting a plant from said plant population that exhibits increased fatigue strength and / or decreased ROS level under fatigue conditions and / or maintains a high level of NADH when compared to a similar non-transgenic plant.

The fatigue-resistant chimeric gene comprises a plant-expressing promoter operably linked to a DNA region encoding a plant functional enzyme of the nicotinamide adenine dinucleotide recovery synthesis selected from denicotinamidase, nicotinate phosphoribosyltransferase, adenonic acid transferase mononucleotide nicotinamide adenine synthase dinucleotide, which are of fungal or yeast-like origin, and to a 3 'terminal region involved in transcription, termination and polyadenylation.

As used herein, the term "a functional enzyme depletes the pathway of nicotine mida adenine dinucleotide recovery synthesis" is an enzyme that when introduced into plants, linked to appropriate control elements such as promoter and terminator region, plant-passive, can be transcribed and translated to produce a functional NAD-recovery synthesis enzyme functional in plant cells. Enzymes (and coding genes) from NAD recovery synthesis are included, which are obtained from a different yeast or fungus than Saccharomyces cerevisae.

The latter proteins are quite suitable for the methods according to the invention as they are less likely to be subjected to enzymatic feedback regulation to which similar plant-derived enzymes may be subjected.

The enzymes involved in the pathway of NAD recovery synthesis comprise the following:

- Nicotinamidase (EC 3.5.1.19), which catalyzes the hydrolysis of

nicotinamide amide, thereby releasing nicotinate and NH3. The enzyme is also known as nicotinamide deaminase, nicotinamide amidase, YNDase or nicotinamide starch hydrolase;

- Nicotinate phosphoribosyltransferase (EC 2.4.2.11), also known as niacin ribonucleotidase, nicotinic glycohydrolase acid monocleotide; nicotinic acid pyrophosphorylase monocleotide; Nicotinic acid phosphoribosyltransferase catalyst for the following reaction: Nicotinate-D-ribonucleotide + diphosphate = nicotinate + 5-phospho-Î ± -D-ribose 1-diphosphate;

- Nucleotide-nicotinate adenylyltransferase (EC 2.7.7.18), also known as deamido-NAD + pyrophosphorylase; denicotinate adenylyltransferase mononucleotide; deamidonicotinamide adeninpyrophosphorylase dinucleotide; NaMT-ATase; nicotinic acid adenylyl transferase mononucleotide, catalyst for the following reaction: ATP + nicotinate ribonucleotide = diphosphate + deamido-NAD +

- NAD synthase (EC 6.3.1.5), also known as NAD synthase; NAD + synthase; nicotinamide adenine synthetase dinucleotide; diphospopyridine synthetase nucleotide, catalyst for the following reaction:

Deamido-NAD + + ATP + NH3 = AMP + diphosphate + NAD +.

In one embodiment of the invention, the coding regions that encode the different enzymes of the NAD retrieval pathway comprise a nucleotide sequence encoding the proteins as amino acid sequences as set forth below.

Suitable nucleotide sequences encoding a single-tinamidase similar to Saccharomyces cerevisae PNC1 but of fungal or yeast origin include a nucleotide sequence encoding a nicotinamidase comprising a selected amino acid sequence from:

- amino acid sequence of accession number XP_444840 (Candida glabrata);

- accession amino acid sequence XP_456073 (Kluyveromyces lactis);

- amino acid sequence accession number NP_986013 (Eremothecium gossypii);

- amino acid sequence of accession number XP_888958 (Candida albicans);

- amino acid sequence of accession number XP500320 (Yarrowia lipolytica);

- amino acid sequence XP389372 (Giberella zeae);

- amino acid sequence of accession number XP_749509 (Aspergillus fumigatus);

- amino acid sequence of accession number XP_712112 (Candida albicans);

- amino acid sequence of accession BAE56421 (Aspergillus oryzae);

- accession amino acid sequence XP_567125 (Cryptococcus neofomans);

- amino acid sequence accession number XP_964547 (Neurospora crassa);

- amino acid sequence of accession number XP_712135 (Candida albicans).

Suitable nucleotide sequences encoding a Saccharomyces cerevisae-like NAD (+) synthetase similar to Qnsl, but originating from fungi, include a nucleotide sequence encoding a NAD (+) synthetase comprising a selected amino acid sequence from:

- amino acid sequence of accession number XP_448179 (Candida glabrata);

- amino acid sequence accession number XP_453643 (Kluyveromyces lactis);

- amino acid sequence accession number NP_987024 (Eremothecium gossypii);

- amino acid sequence of accession number XP_500272 (Yarrowia lipolytica);

- amino acid sequence of accession number XP_722371 (Candida albicans);

- amino acid sequence accession number XP_456405 (Debaromyces hansenii);

- Accession number amino acid sequence BAE61562 (Aspergillus oryzae) - Accession number amino acid sequence XP_759702 (Ustilago maydis);

- amino acid sequence of accession number EAL18079 (Cryptococcus neoformans);

- accession number amino acid sequence NP_587771 (Schizosaccharomyces pombe);

- amino acid sequence of accession number XP_681472 (Aspergillus nidulans);

- amino acid sequence of accession number XP_959191 (Neurospora crassa);

- accession amino acid sequence XP_567726 (Cryptococcus neoformans);

- amino acid sequence of accession number EAQ90706 (Chaetomium globosum);

- accession amino acid sequence XP_387574 (Giberella zeae);

- amino acid sequence of accession number XP_748008 (Aspergillus fumigatus);

- amino acid sequence of accession number XP_361704 (Magnaporthe grisea).

Suitable nucleotide sequences encoding a nicotinic acid adenylyltransferase mo-nonucleotide, similar to Saccharomyces cerevisae NMA1, but of fungal origin include a nucleotide sequence encoding an adeylnyltransferase acid mononucleotide, comprising an amino acid sequence selected:

- amino acid sequence of accession number Q06178;

- amino acid sequence of accession number XP_444815 (Candida glabrata);

- amino acid sequence accession number NP_986687 (Eremothecium gossypii);

- Accession amino acid sequence XP_453005 (Kluyveromyces lactis);

- amino acid sequence accession number XP_458184 (Debaromyces hansenii);

- amino acid sequence of accession number XP_718656 (Candida albicans);

- amino acid sequence of accession number XP_504391 (Yarrowia lipolytica);

- accession number amino acid sequence NP_592856 (Schizosaccharomyces pombe);

- amino acid sequence accession number XP_762639 (Ustilago maydis);

- Accession amino acid sequence XP_571297 (Cryptococcus neoformans);

- amino acid sequence of accession BAE57070 (Aspergillus oryzae);

- amino acid sequence of accession number XP_750776 (Aspergillus fumigatus);

- accession amino acid sequence XP_659349 (Aspergillus nidulans);

- amino acid sequence accession number XP_389652 (Giberella zeae);

- amino acid sequence of accession number XP_957634 (Neurospora crassa);

- accession amino acid sequence XP_363364 (Magnaporthe grisea);

- amino acid sequence accession number XP_758179 (Ustilago maydis);

- amino acid sequence of accession number EAQ85219 (Chaetomium globosum).

Suitable nucleotide sequences encoding a single-tinate phosphoribosyltransferase, similar to Saccharomyces cerevisae NPT1, but of fungi or yeast origin, include a denucleotide sequence encoding a nicotinate phosphoribosyltransferase, comprising an amino acid sequence selected from:

- amino acid sequence of accession number CAA85352 (Saccharomyces cerevisae);

- amino acid sequence of accession number XP_448893 (Candida glabrata);

- amino acid sequence accession number XP_453357 (Kluyveromyces lactis);

- amino acid sequence accession number NP_983562 (Eremothecium gossypii);

- amino acid sequence accession number XP_462577 (Debaromyces hansenii);

- amino acid sequence of accession number XP_889008 (Candida albicans);

- amino acid sequence of accession number XP_500338 (Yarrowia lipolytica);

- amino acid sequence of accession number XP_746744 (Aspergillus fumigatus);

- amino acid sequence of accession BAE64333 (Aspergillus oryzae);

- amino acid sequence of accession number XP_965789 (Neurospora crassa);

- amino acid sequence of accession number EAQ93453 (Chaetomium globosum);

- amino acid sequence of accession number XP_682385 (Aspergillus nidulans);

- amino acid sequence of accession number AAN74808 (Gibberella moniliformis);

- amino acid sequence of accession number Q9UTK3;

- amino acid sequence of accession number XP_361075 (Magnaporthe grisea);

- amino acid sequence of accession number EAL18922 (Cryptococcus neoformans);

- amino acid sequence accession XP_568039 (Cryptococcus neoformans);

- accession amino acid sequence XP_760597 (Ustilago maydis);

Suitable nucleotide sequences encoding a nicotinic acid adenylyltransferase mo-nonucleotide, similar to Saccharomyces cerevisae NMA2, but of fungal or yeast origin include a nucleotide sequence encoding an adenylyltransferase acid mononucleotide comprising a amino acids selected from:

- accession number amino acid sequence NP_011524;

- amino acid sequence of accession number XP_444815 (Candida glabrata);

- amino acid sequence accession number NP_986687 (Eremothecium gossypii);

- Accession amino acid sequence XP_453005 (Kluyveromyces lactis);

- amino acid sequence accession number XP_458184 (Debaromyces hansenii);

- amino acid sequence of accession number XP_718656 (Candida albicans);

- amino acid sequence of accession number XP_504391 (Yarrowia lipolytica);

- accession number amino acid sequence NP_592856 (Schizosaccharomyces pombe);

- amino acid sequence accession number XP_762639 (Ustilago maydis);

- Accession amino acid sequence XP_571297 (Cryptococcus neoformans);

- Accession amino acid sequence BAE57070 (Aspergillus oryzae) - Accession amino acid sequence XP_750776 (Aspergillus fumigatus);

- accession amino acid sequence XP_659349 (Aspergillus nidulans);

- amino acid sequence accession number XP_389652 (Giberella zeae);

- amino acid sequence of accession number XP_957634 (Neurospora crassa);

- accession amino acid sequence XP_363364 (Magnaporthe grisea);

- amino acid sequence accession number XP_758179 (Ustilago maydis);

- amino acid sequence of accession number EAQ85219 ((Chaetomium globosum).

All amino acid sequences referred to by their accession numbers are incorporated herein by reference.

However, it should be clear that alternatives to these sequences, including insertions, deletions and substitutions thereof can also be used for the same purpose. The described sequence alternatives will have a sequence identity that is preferably about 80%, or 85%, or 90% or 95%, with identified NAD retrieval pathway enzyme sequences. Preferably, these alternatives will be functional proteins with the same enzymatic activity as NAD recovery pathway enzymes. For purposes of the present invention, the "sequence identity" of two correlated α-nucleotide sequences or α-amino acid sequences, expressed as a percentage, refers to the number of positions of the two optimally aligned sequences that have identical (x100) divided residues. by the number of positions compared. A spacing, that is, a position in an alignment where a residue is present in one sequence but not the other, is considered to be a position with non-identical residues. The alignment of two sequences is performed by the Needleman and Wunsch algorithm (Needleman and Wunsch, 1970). The above sequence alignment, when computer aided, can be conveniently performed using a standard program such as GAP, which is part of Wisconsin Pac-kage Version 10.1 (Genetics Computer Group, Madision, Wisconsin, USA) , using the default marking array, with a clearance creation penalty of 50 and a clearance extension penalty of 3.

Homologous nucleotide sequences from other fungi or yeast-like organisms can also be identified and isolated by hybridization under harsh conditions, using identified NAD recovery pathway enzyme-coding denucleotide sequences as probes.

The term "harsh hybridization conditions" as used herein means that hybridization will generally occur if at least 95%, preferably at least 97% sequence identity exists between the probe and the target sequence. Examples of severe hybridization conditions include overnight incubation in a solution comprising 50% formamide, 5 χ SSC (150 mM NaCl, 15mM trisodium citrate), 50 mM sodium phosphate (pH 7.6) Χ Denhardt's solution, 10% dextran sulfate and 20 μg / mL of sheared and denatured DNA vehicle, such as salmon sperm DNA, followed by washing of the hybridization support in 0.1 χ SSC, a temperature of about 65 ° C, preferably twice, for about 10 minutes. Other hybridization and washing conditions are well known and exemplified by Sambrooke others, "Molecular Cloning: A Laboratory Manual", second edition, ColdSpring Harbor, NY (1989), particularly in Chapter 11.

The methods of the invention may be used to obtain plants tolerant to different types of fatigue inducing conditions, particularly abiotic fatigue conditions, including flooding, high light conditions, high levels of UV radiation, increased levels of hydrogen peroxide, dry weather conditions, high or low temperatures, increased salinity conditions. The methods of the present invention may also be used to reduce the level of ROS of plant cells growing under adverse conditions, particularly abiotic fatigue conditions, including flooding, high light conditions, high levels of UV radiation, increased levels of hydrogen peroxide, dry weather conditions, high or low temperatures, increased salinity conditions, etc. The ROS level or NADH level can be determined using methods known in the art, including those described in Example 3.

Using the methods described herein, plants can be obtained in which the level of ROS is equal to or less than in control plants under untired conditions such as, but not limited to, a low light condition. In these plants, under non-fatigued conditions, the ROS level may range from 50% to 100% compared to the level of control plants under low light conditions, more particularly from about 60% to about 85%. . The ROS level in these plants under fatigue conditions is about 50% to 80% compared to the ROS level in control plants under fatigue conditions, corresponding to about 60 to 80% of the ROS level in control plants under non-fatigued conditions. . Similarly, the level of NADH in these plants is equal to or higher than in control plants under non-fatigued conditions, such as, but not limited to, a low light condition. In such plants, under non-fatigued conditions, the NADH level may range from 100% to 160% relative to the NADH level of control plants under low light conditions, more particularly from about 120% to about 140%. The NADH level in these plants under fatigue conditions is about 200% to 300% compared to the NADH level in the control plants under fatigue conditions, corresponding to about 100 to 160% of the NADH level in the control plants under non-fatigued conditions. fatigued.

Methods for obtaining transgenic plants are not really critical to the present invention and any suitable transformation and regeneration methods for a particular plant species may be used. Such methods are well known in the art and include Agrobacterium-mediated transformation, piston-a-particle release, microinjection, intact cell electroporation, polyethylene glycol-mediated protoplast transformation, protoplast electroporation, liposome-mediated transformation, mediated by silicon wires, etc. Transformed cells obtained in this manner can then be regenerated into mature fertile plants.

The obtained transformed plant can be used in a conventional breeding scheme for the production of more transformed plants having the same characteristics or for introducing the chimeric gene according to the invention, in other varieties of the same species or in correlated plant species or in plants. hybrids. Seeds obtained from the transformed plants contain the chimeric genes of the invention as a stable genomic insertion and are also included in the present invention.

It will be apparent that the different fatigue-resistant chimeric genes described herein, with DNA regions encoding different enzymes of the NAD retrieval pathway, may be combined within one plant cell or plant to further increase tolerance to the disease. of plants that comprise the chimeric genes. Thus, in one embodiment of the invention, plant and plant cells are provided, comprising at least two fatigue resistant chimeric genes, each comprising a different coding region.

Transgenic plant cells and plant lines according to the invention may further comprise chimeric genes that reduce expression of endogenous PARP and / or PARG genes, as described in WO 00/04173 and PCT / EP 2004/003995. Such additional chimeric genes may be introduced, for example, by cross-bracing transgenic plant lines of the present invention with transgenic plants containing chimeric PARP and / or PARG gene expression reducing genes. Transgenic plant cells or plant lines may also be obtained by introducing or transforming the chimeric genes of the invention into transgenic plant cells containing the chimeric PARP and / or PARG gene expression reducing genes or vice versa. For purposes of the invention, the promoter is a passive per-plant expression promoter. As used herein, the term "passive per-plant expression promoter" means a DNA sequence that is capable of controlling (initiating) transcription in a plant cell. This includes any plant-origin promoter, but also any non-plant promoter that is capable of directing transcription into a plant cell, that is, certain promoters of viral or bacterial origin, such as , the CaMV35S promoter (Harpster et al., 1988, Moi Gen. Genet.212, 182-190), the underground clover virus promoter No. 4 or N-7 (WO 96/06932), or promoters T-DNA gene, but also tissue-specific or organ-specific promoters, including, but not limited to, seed-specific promoters (e.g., cited in WO 89/03887), specific promoters. primitive organs (An et al., 1996, The Plant Cell 8, 15-30), stem-specific promoters (Keller et al., 1988, EMBO J. 7, 3625-3633), leaf-specific promoters (Hudspeth et al. 1989, Plant MolBiol 12, 579-589), mesophil-specific promoters (such as promo-tore s Light-Inducible Rubisco), root-specific promoters (Keller et al., 1989, Genes Deveri. 3, 1639-1646), tubercle-specific promoters (Keil et al., 1989, EMBO J. 8, 1323-1330), vascular endowed specific promoters (Peleman et al., 1989, Gene 84, 359-369), selective promoters. stamens (patent documents WO 89/10396 and WO92 / 13956), dehiscence zone specific promoters (patent document WO 97/13865) and others.

The chimeric genes of the invention may also be provided with a plant functional nuclear localization signal ("NLS") operably linked to the DNA region encoding an NAD retrieval enzyme, such as the SV40 NLS.

Upon reading this document, one of ordinary skill in the art will immediately imagine that similar effects can be obtained with respect to increased fatigue resistance whenever natural plant variations are obtained when endogenous genes encoding NAD recovery pathway enzymes are obtained. more active or expressed at a higher level. These plant variations can be obtained by subjecting a plant population to mutagenesis, such as, but not limited to, EMS mutagenesis, followed by a selection of an increased activity of any of the NAD recovery pathway enzymes or a combination thereof. .

Also, it should be immediately apparent that a population of different varieties or crops may be selected for increased tolerance to the aforementioned fatigue conditions, generally or particularly selected for abiotic fatigue, followed by a tolerance correlation. increased fatigue conditions with the presence of a particular allele or any of the endogenous genes encoding a NAD recovery pathway enzyme. These alleles can then be introduced into a plant of interest by mating if the species are sexually compatible, or can be identified using standard techniques as described herein (including PCR hybridization or amplification) and introduced using recombinant DNA technology. . The introduction of particularly desired alleles using breeding techniques can be accompanied by using specific allele markers of interest.

The methods and media described herein are believed to be suitable for all plant and plant cells, dicotyledonous and monocotyledonous plant cells and plants which include, but are not limited to, Brassicaceae, rapeseed, wheat, corn or millet, barley, sunflower, rice, oats, sugar cane, soybeans, vegetables (including chicory, lettuce, tomato), smoke, potato, beet, papaya, pineapple, mango, Arabidose thaliana, but also, plants used in horticulture, floriculture or reforestation.

As used herein, the term "comprising" shall be construed as specifying the presence of the indicated characteristics, integers, steps or components referred to in said characteristics, but does not preclude the presence or addition of one or more characteristics, numbers. integers, steps or components, or groups thereof. Thus, for example, a nucleic acid or protein comprising a denucleotide or amino acid sequence may comprise more nucleotides or amino acids than those actually quoted, that is, being embedded in a larger nucleic acid or protein. A chimeric gene comprising a DNA region that is functionally or structurally defined may comprise additional DNA regions, etc.

The following non-limiting examples describe the construction of chimeric genes to increase fatigue strength in plant cells and plants, as well as the use of these genes.

Unless otherwise indicated in the Examples, all recombinant DNA techniques are performed according to standard protocols as described by Sambrook et al (1989), "Molecular Cloning: A Laboratory Manual", Second Edition, Cold Spring Harbor LaboratoryPress, NY and Ausubel et al. Volumes 1 and 2 (1994) "Current Proto-cols in Molecular Biology, Current Protocols", USA. Standard materials and methods for plant molecular work are described in the publication "Plant Molecular Biology Labfax" (1993), by R.D.D. Croy, jointly published by BIOS Scientific Publications Ltd (UK) and Blackwell Scientific Publications, UK. Other references to standard molecular biology techniques include, Sambrook and Russell (2001) "Molecular Cloning: A Laboratory Manual", Third Edition, Cold Spring Harbor Laboratory Press, NY, Brown Volumes III (1998), "Molecular Biology" LabFax ", Second Edition, Acade-mic Press (UK). Standard materials and methods for polymerase chain reactions can be found in Dieffenbach and Dveksler (1995), "PCR Primer: A Laboratory Manual", Cold Spring Harbor LaboratoryPress, and in McPherson et al. (2000) "PCR. - Basics: FromBackground to Bench ", First Edition, Springer Verlag, Germany.

Throughout the descriptive report, reference is made to the following entries in the sequence listing: SEQ ID NO. 1: XP_444840 (Candida glabrata) SEQ ID NO. 2: XP_456073 (Kluyveromyces lactis) SEQ ID NO. 3: NP_986013 (Eremothecium gossypii)

SEQ ID NO. 4: XP_888958 (Candida albicans)

SEQ ID NO. 5: XP500320 (Yarrowia lipolytica)

SEQ ID NO. 6: XP389372 (Giberella zeae)

SEQ ID NO. 7: XP_749509 (Aspergillus fumigatus)

SEQ ID NO. 8: XP_712112 (Candida albicans)

SEQ ID NO. 9: BAE56421 (Aspergillus oryzae)

SEQ ID NO. 10: XP_567125 (Cryptococcus neofomans)

SEQ ID NO. 11: XP_964547 (Neurospora crassa)

SEQ ID NO. 12: XP_712135 (Candida albicans)

SEQ ID NO. 13: XP_448179 (Candida glabrata)

SEQ ID NO. 14: XP_453643 (Kluyveromyces lactis)

SEQ ID NO. 15: NP_987024 (Eremothecium gossypii)

SEQ ID NO. 16: XP_500272 (Yarrowia lipolytica)

SEQ ID NO. 17: XP_722371 (Candida albicans)

SEQ ID NO. 18: XP_456405 (Debaromyces hansenii)

SEQ ID NO. 19: BAE61562 (Aspergillus oryzae)

SEQ ID NO. 20: XP_759702 (Ustilago maydis)

SEQ ID NO. 21: EAL18079 (Cryptococcus neoformans)

SEQ ID NO. 22: NP_587771 (Schizosaccharomyces pombe)

SEQ ID NO. 23: XP_681472 (Aspergillus nidulans)

SEQ ID NO. 24: XP_959191 (Neurospora crassa)

SEQ ID NO. 25: XP_567726 (Cryptococcus neoformans)

SEQ ID NO. 26: EAQ90706 (Chaetomium globosum)

SEQ ID NO. 27: XP_387574 (Giberella zeae)

SEQ ID NO. 28: XP_748008 (Aspergillus fumigatus)

SEQ ID NO. 29: XP_361704 (Magnaporthe grisea)

SEQ ID NO. 30: Q06178

SEQ ID NO. 31: XP_444815 (Candida glabrata)

SEQ ID NO. 32: NP_986687 ((Eremothecium gossypii)

SEQ ID NO. 33: XP_453005 (Kluyveromyces lactis)

SEQ ID NO. 34: XP_458184 (Debaromyces hansenii) SEQ ID NO. 35: XP_718656 (Candida albicans)

SEQ ID NO. 36: XP_504391 (Yarrowia lipolytica)

SEQ ID NO. 37: NP_592856 (Schizosaccharomyces pombe)

SEQ ID NO. 38: XP_762639 (Ustilago maydis)

SEQ ID NO. 39: XP_571297 (Cryptococcus neoformans)

SEQ ID NO. 40: BAE57070 (Aspergillus oryzae)

SEQ ID NO. 41: XP_750776 (Aspergillus fumigatus)

SEQ ID NO. 42: XP_659349 (Aspergillus nidulans)

SEQ ID NO. 43: XP_389652 (Giberella zeae)

SEQ ID NO. 44: XP_957634 (Neurospora crassa)

SEQ ID NO. 45: XP_363364 (Magnaporthe grisea)

SEQ ID NO. 46: XP_758179 (Ustilago maydis)

SEQ ID NO. 47: EAQ85219 ((Chaetomium globosum)

SEQ ID NO. 48: CAA85352 (Saccharomyces cerevisae)

SEQ ID NO. 49: XP_448893 (Candida glabrata)

SEQ ID NO. 50: XP_453357 (Kluyveromyces lactis)

SEQ ID NO. 51: NP_983562 (Eremothecium gossypii)

SEQ ID NO. 52: XP_462577 (Debaromyces hansenii)

SEQ ID NO. 53: XP_889008 (Candida albicans)

SEQ ID NO. 54: XP_500338 (Yarrowia lipolytica)

SEQ ID NO. 55: XP_746744 (Aspergillus fumigatus)

SEQ ID NO. 56: BAE64333 (Aspergillus oryzae)

SEQ ID NO. 57: XP_965789 (Neurospora crassa)

SEQ ID NO. 58: EAQ93453 (Chaetomium globosum)

SEQ ID NO. 59: XP_682385 (Aspergillus nidulans)

SEQ ID NO. 60: AAN74808 (Gibberella moniliformis)

SEQ ID NO. 61: Q9UTK3

SEQ ID NO. 62: XP_361075 (Magnaporthe grisea)

SEQ ID NO. 63: EAL18922 (Cryptococcus neoformans)

SEQ ID NO. 64: XP_568039 (Cryptococcus neoformans)

SEQ ID NO. 65: XP_760597 (Ustilago maydis)

SEQ ID NO. 66: NP_011524.All amino acid sequences referred to by their accession numbers are incorporated herein by reference.

Examples

Example 1: Construction of fatigue resistant chimeric genes and their introduction into plants.

To increase resistance to plant fatigue, a chimeric gene is constructed using conventional techniques, comprising the following DNA fragments, in the order below:

- a cauliflower juxtaposed virus promoter region (CaMV35S);

- a DNA fragment of about 60 bp, corresponding to Cab22L untranslated gene;

- a DNA fragment, as mentioned herein, encoding a fungal or yeast-derived NAD retrieval pathway enzyme other than Saccharomycescerevisae PNC1, NMA1, NMA2 or NPT1;

a fragment of the 3 'untranslated terminus of CaMV 35S transcript (3'35S).

This chimeric gene is introduced into a T-DNA vector between left and right boundary sequences of the T-DNA, along with a selectable marker gene.

T-DNA vectors are introduced into Agrobacterium strains comprising a helper plasmid Ti using conventional methods. Chimeric genes are introduced into plants using a conventional transformation method. Transgenic plants exhibit greater fatigue resistance than their counterpart plants without the presence of transgenes. Listing Sequence <110> Bayer BioScience NVDe Bloek, MareMetzlaff, MiehaelGossele, Veronique <120> Fatigue Resistant Plants. <130> BCS06- 2004 <150> EP06075671.5 <151> 2006-03-21 <150> US60 / 784,179 <151> 2006-03-21 <150> EP06075700.2 <151> 2006-03-22 <160> 66

<170> PatentIn version 3.3 <210> 1 <211> 211 <212> PRT

<213> Candida glabrata <400> 1

Met Lys Thr Read Leu Val Ile Asp Val Gln Asn Asp Phe Ile Thr Pro1 5 10 15

Asp His Ser Met Tyr Val Gln Gly Glu Glu Val Val Ser Ile

20 25 30

Val Glu Read Met Lys Asp Pro Gln Trp His Arg Val Val Val Ser Arg

35 40 45

Asp Trp His Pro Gln Asn His Ile Ser Phe Alys Lys Asn His Gly Val

50 55 60

Glu Asp Tyr Thr Glu Thr Tyr Lys Be Pro Arg Pro Gly Asp Asp65 70 75 80

Ser Thr Gln Pro Wing Thr Leu Trp Pro Val His Val Val Gln Asn Thr85 90 95Arg Gly Wing Gln Leu Wing Pro Asp Ile Leu Glu Leu Val Asn Ser Lys

100 105 110

His Ile Lys Ile Val Asp Lys Gly Tyr Read Asn Cys Glu Tyr Tyr

115 120 125

Ser Ala Phe Asn Asp Thr Trp Glu Trp His Lys Thr Glu Read Asp Glu

130 135 140

Tyr Leu Lys Lys His His Thr Thr Glu Val Tyr Val Val Gly Leu Ala145 150 155 160

Read Asp Phe Cys Val Lys Asn Thr Wing Ile Ser Wing Lys Wing Read Gly

165 170 175

Tyr Asp Thr Thr Ile Read Lys Asp Tyr Thr Lys Pro Ile Tyr Thr Asp

180 185 190

Glu Asp His Gln Gln Gln Leu Glu Lys Asp Leu Lys Glu His Asn Val

195 200 205

Lys Val Lys210 <210> 2 <211> 218 <212> PRT

<213> Kluyveromyces lactis <400> 2

Met Gly Wing Arg Wing Leu Leu Val Ile Asp Ile Gln Asn Asp Phe Leu1 5 10 15

Pro Pro Lys Gly Ser Leu Val Wing Gln Asp Gly Asp Thr Ile Ile Asp

20 25 30

Pro Val Ile Gln Leu Read Gln Asp Gln Asp Trp Asp Cys Val Wing Met

35 40 45

Thr Lys Asp Trp His Pro Asp His Ile Ser Phe Ala Lys Asn His

50 55 60

Gly Leu Pro Asp Phe Be Be Phe Thr Tyr Asp Be Pro Val Pro Gly65 70 75 80

Ser Thr Glu Lys Gln Ser Thr Thr Leu Trp Pro Val His Cys Val Gln85 90 95

Glu Thr Trp Gly Ser Glu Val Pro Glu Lys Leu Leu Wing Glu Ile Leu

100 105 110

Lys Leu Lys Val Pro His Lys Ile Val Asn Lys Gly Tyr Leu Ser Asp

115 120 125

Arg Glu Tyr Tyr Be Gly Phe Asn Asp Ile Trp Asn Asp His His Thr

130 135 140

Glu Asu Asp Wing Phe Phe Lys Glu Asn Asp Val Thr Glu Ile Tyr Val145 150 155 160

Val Gly Read Ala Phe Asp Phe Cys Val Lys Asn Ser Ala Ile Ser Ala

165 170 175

Wing Asn Read Gly Tyr His Val Thr Ile Read Lys Asp Tyr Thr Lys Wing

180 185 190

Ile Wing Asn Asp Leu Gln Be Ile Glu Be Phe Ile Gln Glu Leu Wing

195 200 205

Lys Asn Glu Val Ser Val Gln Glu Ser Ile

210 215

<210> 3 <211> 218 <212> PRT

<213> Eremothecium gossypii <400> 3

Met Ser Lys Wing Leu Ile Val Val Asp Val Gln Asn Asp Phe Asp1 Wing 5 10 15

Wing Arg Gly Wing Leu Wing Val Pro Gly Wing His Glu Ile Val Gln Pro

20 25 30

Leu Wing Glu Leu Wing Gln Asp Pro Arg Trp Wing Tyr Val Wing Met Thr

35 40 45

Arg Asp Trp His Pro Pro Asp His Val Ser Phe Wing Asp Thr His Gly

50 55 60

Arg Pro Pro Phe Be Pro Tyr Met Tyr His Pro Pro Gly Val Arg65 70 75 80Ala Pro Pro Gln Wing Gly Thr Read Trp Pro Thr His Cys Val Gln Gly

85 90 95

Ser Trp Gly Wing Gln Leu Pro Wing Gln Leu Wing Wing Wing Arg Wing

100 105 110

Pro His Ser Val Val Asp Lys Gly Val Trp Pro Asp Arg Glu Cys Tyr

115 120 125

Be Wing Phe Glu Asp Ile Trp Wing Asp Arg Be Ser Gly Read Asp Gly

130 135 140

Leu Leu Arg Be His Gly Val Lys His Val Tyr Val Wing Gly Leu Wing Wing145 150 155 160

Read Asp Tyr Cys Val Lys Be Thr Wing Ile Be Wing Wing Arg Leu Gly

165 170 175

Tyr Thr Thr Thr Ile Leu Asp Tyr Thr Arg Wing Ile Wing Wing Asp

180 185 190

Gln Wing Be Met Wing Arg Read Le Be Ser Asp Leu Wing Gly His Gln Val

195 200 205

Wing Read Cys Glu Gly Wing Glu Pro Leu Pro

210 215

<210> 4 <211> 244 <212> PRT

<213> Candida albicans <400> 4

Met Lys Lys Thr Wing Leu Ile Val Val Asp Leu Gln Glu Asp Phe Leu1 5 10 15

Pro Pro Asn Gly Be Read Ala Ile Wing Asn Gly Arg Be Val Ile Pro

20 25 30

Lys Ile Asn Gln Read Leu Pro To Be Gln Asp Asn His Ser Lys Phe Asp

35 40 45

Trp Ser Leu Ile Val Wing Thr Gln Asp Trp His Pro Asn His Thr

50 55 60

Ser Phe Ala Ser Gln His Glu Asn Val Ala Pro Phe Thr Glu Ile Glu65 70 75 80

Phe Ile His Pro Glu Lys Lys Read Asp Pro Lys Thr Asn Gln Pro Ile

85 90 95

Val Met Asn Gln Ile Val Trp Pro Asp His Cys Val Gln Gly Thr Lys

100 105 110

Gly Wing Gln Leu Glu Pro Be Phe Wing Asn Gln Phe Glu Lys Leu Thr

115 120 125

Lys Gln Asp Asp Asn Asn Thr Wing Pro Cys Lys Ile Val Lys Lys Gly

130 135 140

Tyr Leu Pro Asp Arg Glu Tyr Tyr Be Cys Phe Gln Asp Cys Trp Gly145 150 155 160

Read His His Thr Glu Read His Ile Asp Read His Read His Glu Tyr Asp Ile Glu

165 170 175

Asn Val Val Phe Val Gly Leu Wing Tyr Asp Val Val Phe Leu Ser Ser

180 185 190

Wing Ile Asp Being Wing Lys Asn Gly Phe Lys Thr Phe Val Leu Lys Asn

195 200 205

Tyr Cys Glu Ser Val Tyr Pro Glu Lys Ile Asn Asp Thr Asp Lys Leu

210 215 220

Phe Ile Asp Asn Gly Val Thr Ile Val Asp Asn Asp Glu Lys Phe Asp225

Ser Leu Phe Lys

<210> 5 <211> 203 <212> PRT

<213> Yarrowia lipolytica <400> 5

Met Wing Wing Leu Ile Ile Val Asp Leu Gln Asn Asp Phe Leu Pro Gly1 5 10 15

Gly Ser Leu Wing Val Val Asp Gly Asn Asp Ile Ile Pro Ile Val Gln20 25 30Lys Leu Wing Asp Be Gly Lys Tyr Lys Phe Val Val Wing Thr Lys Asp

35 40 45

Be His Pro Gln Asp His Thr Be Phe Ala Wing Asn His Gly Wing Glu

50 55 60

Pro Phê Thr Be Ile Thr Phe Lys His Pro Asn Ser Asp Lys Gln Val65 70 75 80

Asp His Thr Val Trp Pro Val His Cys Val Glu Gly Thr Be Gly Wing

85 90 95

Asp Tyr Pro Pro Be Phe Asp Be Ser Asn Val Gln Wing Leu Val Arg

100 105 110

Lys Gly Tyr Read Gln Asp Arg Glu Tyr Tyr Ser Gly Phe Glu Asp Val

115 120 125

Trp Gly Ile His Lys Thr Glu Read His Asp Read Leu Read Gln Gln Asn Gly

130 135 140

Val Thr Glu Val Asp Val Val Gly Leu Wing Phe Asp Tyr Cys Val Phe145 150 155 160

Asn Thr Wing Lys Asp Wing Wing Lys Arg Gly Tyr Lys Thr Thr Val Ile

165 170 175

Arg Glu Wing Thr Lys Pro Val Asp Pro Be Ser Glu Lys Lys Ile Val

180 185 190

Wing Ser Leu Glu Glu Wing Gly Val His Val Val195 200

<210> 6 <211> 223 <212> PRT

<213> Giberella zeae <400> 6

Met Thr Be Gln Lys Be Phe Lys Pro Wing Read Ile Ile Val Asp Phe1 5 10 15

Gln Glu Asp Phe Pro Cys Pro Asn Gly Serve Read Wing Val Pro Glu Gly

20 25 30

Arg Thr Ile Ala Pro Thr Ile Asn Thr Leu Thr Ala Leu Pro Phe His35 40 45

Ile Read Ile Read Wing Thr Lys Asp Phe His Pro To Be His Ile Be Phe

50 55 60

Wing Be Asn His Pro Be Be Thr Pro Tyr Thr Be Thr Thr Ile65 70 75 80

Thr His Pro Arg Asp Be Ser Arg Be Tyr Thr Thr Thr Read Trp Pro

85 90 95

Thr His Cys Val Gln Gly Thr Pro Gly Wing Asp Leu Val Pro Glu Leu

100 105 110

Asp Val Ser Arg Read His Wing Val Ile Glu Lys Gly Gln Asp Lys Arg

115 120 125

Val Glu Met Tyr Ser As Phe Tyr Asp Pro Phe Arg Val Ser As As

130 135 140

Gly Leu Wing Gly Met Leu Gly Glu Gln Asn Val Thr Asp Val Phe Val145 150 155 160

Val Gly Leu Wing Wing Asp Phe Cys Val Lys Wing Wing Thr Wing Glu Wing Wing

165 170 175

Val Lys Glu Gly Tyr Be Thr Trp Ile Val Asn Glu Gly Thr Lys Pro

180 185 190

Val Met Pro Asp Lys Trp Asp Glu Cys Arg Lys Gly Met Glu Asp Met

195 200 205

Gly Ile Lys Phe Thr Be Val Wing Asn Wing Val Asp Lys Phe Lys

210 215 220

<210> 7 <211> 235 <212> PRT

<213> Aspergillus fumigatus <400> 7

Met Lys Wing Leu Wing Ile Val Val Asp Met Gln Glu Asp Phe Cys Pro1 5 10 15

Pro Asp Gly Be Leu Wing Val Gln Gly Wing Arg Be Ile Wing Pro Leu20 25 30Ile Asn Ser Leu Wing Asn Pro Gly Phe Val Ile Arg Val Wing Be

35 40 45

Gln Asp Tyr His Pro Arg Asp His Val Ser Phe Ala Ser Asn His Pro

50 55 60

Glu Pro Asn Asn Arg Pro Phe Glu Ser Val Ile Gln Met Asn Asn Pro65 70 75 80

Pro Wing Gly Lys Glu Be Glu Thr Lys Glu Arg Leu Trp Pro Val

85 90 95

His Cys Val Gly Gly Thr Lys Gly Ala Thr Ile Ile Pro Glu Ile Asp

100 105 110

Be Ser Lys Ile Asp Read His Val Lys Lys Gly Met Asp Ser Arg Val

115 120 125

Glu Met Tyr Be Wing Phe Be Asp Wing Phe Gly Asn Leu Asp Pro Wing

130 135 140

Val His Thr Gln Ser Val Asp Val Asp Leu Lys Wing Val Leu Wing Glu145 150 155 160

Arg Gly Ile Thr His Val Phe Ser Ala Gly Ile Ala Gly Asp Tyr Cys

165 170 175

Val Lys Tyr Thr Wing Met Asp Wing Arg Wing Wing Gly Phe Lys Be Phe

180 185 190

Read Valu Glu Asp Wing Thr Arg Be Val Asp Be Gly Wing Gly Trp Glu

195 200 205

Glu Wing Arg Arg Glu Cys Glu Wing Gly Val Ser Ile Ile Gln Ser

210 215 220

Asp Gly Pro Glu Ile Wing Wing Leu Thr Wing Ser225 230 235

<210> 8 <211> 165 <212> PRT

<213> Candida albicans <400> 8

Met Lys Lys Thr Wing Leu Ile Val Val Asp Leu Gln Glu Asp Phe Leu15 10 15

Pro Pro Asn Gly Be Read Ala Ile Wing Asn Gly Arg Be Val Ile Pro

20 25 30

Lys Ile Asn Gln Read Leu Pro To Be Gln Asp Asn His Ser Lys Phe Asp

35 40 45

Trp Ser Leu Ile Val Wing Thr Gln Asp Trp His Pro Asn His Thr

50 55 60

Ser Phe Ala Ser Gln His Glu Asn Val Ala Pro Phe Thr Glu Ile Glu65 70 75 80

Phe Ile His Pro Glu Lys Lys Read Asp Pro Lys Thr Asn Gln Pro Ile

85 90 95

Val Met Asn Gln Ile Val Trp Pro Asp His Cys Val Gln Gly Thr Lys

100 105 110

Gly Wing Gln Leu Glu Pro Be Phe Wing Asn Gln Phe Glu Lys Leu Thr

115 120 125

Lys Gln Asp Asp Asn Asn Thr Wing Pro Cys Lys Ile Val Lys Lys Gly

130 135 140

Tyr Leu Pro Asp Arg Glu Tyr Tyr Be Cys Phe Gln Asp Cys Trp Gly145 150 155 160

Tyr Ile Ile Ser Asn165

<210> 9 <211> 234 <212> PRT

<213> Aspergillus oryzae <400> 9

Met Lys Wing Wing Leu Val Val Val Asp Met Gln Glu Asp Phe Cys Pro1 5 10 15

Pro Asn Gly Val Leu Pro Val Gln Glu Gly Arg Wing Ile Wing Pro Ile

20 25 30

Ile Asn Glu Read Leu Wing His Gln Gly Phe Wing Val Arg Val Wing Wing Thr35 40 45Gln Asp Tyr His Pro Val Asp His Ile Be Phe Wing Asn Be His Pro

50 55 60

Arg Pro Asn Asn Arg Pro Phe Glu Ser Val Ile Thr Val Asn Asn Pro65 70 75 80

Gly Lys Pro Wing Glu His Glu Thr Lys Pro Gln Asn Leu Trp Pro Wing

85 90 95

His Cys Val Gly Glu Thr Arg Gly Glu Wing Ile Ile Pro Glu Ile Gln

100 105 110

Thr Asp Asn Ile Asp Leu Tyr Val Lys Lys Gly Met His Ser Gln Val

115 120 125

Glu Met Tyr Ser Wing Phe Wing Asp Wing Phe Gly Wing Val Asp Pro Ser

130 135 140

Ile Thr Asp Gln Ser Val Asp Wing Asp Leu Lys Asp Phe Leu Wing Ser145 150 155 160

Lys Gly Val Thr Asp Val Phe Val Val Gly Leu Wing Gly Asp Tyr Cys

165 170 175

Val Lys His Thr Wing Ile Asp Wing Wing Arg Wing Val Gly Phe Lys Ser Tyr

180 185 190

Val Val Glu Asn Wing Ile Arg Cys Val Val Pro Gly Ser Gly Trp Asp

195 200 205

Gly Wing Lys Arg Glu Leu Arg Glu Wing Gly Val Ser Ile Ile Gln Ser

210 215 220

Asn Gly Pro Glu Ile Ser Gly Leu Wing Ile225 230

<210> 10 <211> 225 <212> PRT

<213> Cryptococcus neoformans <400> 10

Met Wing Be Met Pro Wing Be Thr Wing Read Ile Ile Val Asp Val Gln1 5 10 15

Asn Asp Phe Le Pro Pro Thr Gly Ser Leu Wing Val Pro Asn Gly Arg20 25 30

Glu Val Leu Pro Val Ile Thr Gly Leu Read Asp Arg Lys Trp Asp Trp

35 40 45

Wing Val Val Val Val Ser Gln Asp Tyr His Pro Lys Gly His Ile Ser

50 55 60

Phe Wing Be His Pro Pro Wing Asn Gln Wing Tyr Thr Gln Leu Pro Leu65 70 75 80

Val Asn Wing His Gly Glu Ser Tyr Ile Gln Thr Leu Trp Pro Asp His

85 90 95

Cys Ile Gln Gly Thr Wing Gly Wing Asp Leu Glu Ser Gly Leu Glu Wing

100 105 110

Val Leu Wing Lys Arg Gly Asp Gly Ile His Ser Lys Leu Glu Wing Tyr

115 120 125

Ser Phe Glu Glu Ile Val Pro Pro Lys Thr Ser Glu Leu Glu Wing

130 135 140

Phe Leu Leu Wing Gln Gly Val Asn Lys Val Val Ile Wing Gly Val Ala145 150 155 160

Thr Asp Phe Cys Val Leu Gln Thr Wing Read Be Ser Ile Ser Ser

165 170 175

Phe Pro Thr Read Leu Ile Wing Pro Wing Met Arg Wing Wing Ile Be Pro Glu

180 185 190

Tyr Glu Wing Lys Thr Phe Glu Wing Val Glu Ser Leu Gly Gly Val Ile

195 200 205

Leu Gly Arg Asn Gly Glu Glu Trp Lys Thr Lys Leu Wing Glu Trp Ile210 215 220

Arg225 <210> 11 <211> 297 <212> PRT

<213> Neurospora crassa <400> 11Met Gly Phe Leu Glu Trp Leu Met Pro Pro Ile Phe Asn Val Phe1 5 10 15

Gln Thr Arg Read Leu Glu Asn Gly Gln Gly Wing Gln Pro Glu Pro Asp

20 25 30

Phe Arg Pro Wing Leu Leu Val Val Asp Met Gln Glu Asp Phe Cys Pro

35 40 45

Pro Asn Gly Thr Leu Wing Val Val Gly Gly Arg Ser Ile Thr Pro Leu

50 55 60

Ile Asn Thr Leu Leu Be Ser Pro Leu Phe Val Leu Arg Ile Wing Thr65 70 75 80

Lys Asp Trp His Pro Asn His Ile Be Phe Ala Ser Asn His Asn

85 90 95

His Wing Be Ser Pro Be Cys Pro Cys Asp Be Ser Gly Lys Ala

100 105 110

Ala Ile Pro Phe Read Be Thr Thr Thr His Asn Pro His Asn Pro

115 120 125

Be Glu Be Tyr Thr Thr Arg Leu Trp Pro Be His Cys Ile Wing Asp

130 135 140

Thr Pro Gly Wing Ser Leu Ile Pro Glu Leu Asp Val Ser Lys Ile Asp145 150 155 160

Gln Ile Read Glu Lys Gly Thr Asn Arg Read Le Val Glu Met Tyr Ser Ala

165 170 175

Phe Tyr Asp Pro Phe Thr Be Pro Arg Val Be Asp Ser Gly Leu Wing

180 185 190

His Met Leu Arg Glu Wing Lys Val Thr His Val Tyr Val Val Gly Leu

195 200 205

Wing Asp Wing Tyr Cys Val Trp Ser Thr Wing Met Asp Wing His Asn Glu

210 215 220

Gly Phe Glu Val Thr Val Val Glu Glu Wing Thr Lys Pro Val Asp Glu225 230 235 240

Asp Gly Trp Arg Arg Cys Lys Glu Wing Leu Val Gly Glu Pro Gly Val245 250 255Arg Val Val Arg Arg Trp Glu Gly Glu Glu Val Arg Arg Leu Phe Pro Gly

260 265 270

Gly Leu Val Thr Thr Thr Val Gly Wing Gly Asn Asp Glu Glu Val Val

275 280 285

Glu Glu Glu Glu Glu Glu Glu Glu Lys Ile

290 295

<210> 12 <211> 121 <212> PRT

<213> Candida albicans <400> 12

Met Lys Lys Thr Wing Leu Ile Val Val Asp Leu Gln Glu Asp Phe Leu1 5 10 15

Pro Pro Asn Gly Be Read Ala Ile Wing Asn Gly Arg Be Val Ile Pro

20 25 30

Lys Ile Asn Gln Read Leu Pro To Be Gln Asp Asn His Ser Lys Phe Asp

35 40 45

Trp Ser Leu Ile Val Wing Thr Gln Asp Trp His Pro Asn His Thr

50 55 60

Ser Phe Ala Ser Gln His Glu Asn Val Ala Pro Phe Thr Glu Ile Glu65 70 75 80

Phe Ile His Pro Glu Lys Lys Read Asp Pro Lys Thr Asn Gln Pro Ile

85 90 95

Val Met Asn Gln Ile Val Trp Pro Asp His Cys Val Gln Gly Thr Lys

100 105 110

Gly Wing Gln Leu Glu Pro To Be Phe Wing

115 120

<210> 13 <211> 713 <212> PRT

<213> Candida glabrata <400> 13Met Be His Leu Val Thr Leu Wing Thr Cys Ser Leu Asn Gln Trp Ala1 5 10 15

Read Asp Phe Glu Gly Asn Arg Asp Arg Ile Read Glu As Ile Arg Ile

20 25 30

Wing Lys Glu Arg Gly Wing Arg Leu Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Ser Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Asn Asp Val Cys Leu

50 55 60

His Ser Trp Glu Met Tyr Wing Gln Ile Read Lys Asn Pro Glu Thr His65 70 75 80

Gly Leu Ile Leu Asp Ile Gly Met Pro Leu Leu Read His Lys Asn Val Arg

85 90 95

Tyr Asn Cys Arg Read Leu Read Le Asp Gly Lys Ile Leu Phe Ile Arg

100 105 110

Pro Lys Ile Trp Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Phe

115 120 125

Phe Thr Pro Trp Met Lys Pro Gly Val Val Glu Glu Leu Thr Leu Pro

130 135 140

Pro Met Ile Gln Lys Ile Thr Gly Gln Lys Val Pro Phe Gly Asp145 150 155 160

Val Ile Wing Asn Thr Read Asp Thr Cys Ile Gly Glu Wing Thr Cys Glu

165 170 175

Glu Val Phe Thr Pro Gln Be Pro His Ile Wing Met Be Read Asp Gly

180 185 190

Val Glu Ile Ile Thr Asn Be Ser Gly Be His His Glu Leu Arg Lys

195 200 205

Leu Asn Lys Arg Leu Glu Leu Ile Leu Asn Gly Thr Gly Arg Cys Gly

210 215 220

Gly Val Tyr Leu Tyr Asn Wing Gln Lys Gly Cys Asp Gly Asp Arg Leu225 230 235 240

Tyr Tyr Asp Gly Cys Wing Leu Ile Wing Ile Asle Gly Lys Ile Leu Wing245 250 255Gln Gly Lys Gln Phe Ser Leu Asp Asp Val Glu Val Val Thr Ala Thr

260 265 270

Val Asp Leu Glu Glu Val Arg Asn His Arg Wing Asn Val Met Ser Arg

275 280 285

Gly Leu Gln Be Ser Leu Wing Asp Leu Lys Tyr Glu His Ile Asp Val

290 295 300

Glu Ile Glu Leu Wing Pro Arg Gly Ser Arg Phe Asn Pro Lys Ile Thr305 310 315 320

Pro Thr Lys Be Arg Asp Val Thr Tyr His Thr Pro Glu Glu Glu Ile

325 330 335

Wing Read Gly Pro Wing Cys Trp Read Trp Asp Tyr Ile Arg Arg Cys Asn

340 345 350

Gly Thr Gly Tyr Phe Gave Pro Read Gly Gly Gly Ile Asp Be Cys Wing

355 360 365

Thr Wing Met Ile Ile His Be Met Cys Arg Read Val His Lys Cys Wing

370 375 380

His Glu Gly Asn Asp Leu Val Leu Lys Asp Ile Arg Arg Ile Thr Arg385 390 395 400

Be Pro Asp Asp Trp Ile Pro Glu Asn Pro Gln Glu Ile Wing Asn Lys

405 410 415

Met Phe His Thr Cys Phe Met Gly Thr Glu Asn Ser Be Val Glu Thr

420 425 430

Arg Ser Arg Ser Lys Gln Leu Wing Glu Lys Ile Gly Ser Tyr His Val

435 440 445

Asp Leu Asn Met Asp Gly Leu Val Ser Ser Val Val Ser Leu Phe -Glu

450 455 460

Val Wing Thr Gly Arg Lys Pro Ile Phe Lys Ile Phe Gly Gly Ser Gln465 470 475 480

Ile Glu Asn Leu Wing Wing Leu Gln Asn Ile Glu Wing Arg Leu Arg Met Val

485 490 495

Leu Wing Tyr Leu Phe Wing Gln Leu Leu Pro Trp Val Arg Gly Ile Pro500 505 510Asn Ser Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Cys

515 520 525

Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Be Ser Asa Val Val Asn

530 535 540

Pro Ile Gly Gly Ile Be Lys Thr Asp Read Lys Gly Phe Ile Lys Tyr545 550 555 560

Wing Be Glu Glu Tyr Asp Met Pro Ile Read Asp Glu Phe Read Asn Wing

565 570 575

Thr Pro Thr Wing Glu Leu Glu Pro Ile Thr Lys Asp Tyr Val Gln Ser

580 585 590

Asp Glu Arg Asp Met Gly Met Thr Tyr Glu Glu Read Ser Val Phe Gly

595 600 605

Tyr Leu Arg Lys Val Glu Lys Cys Gly Pro Tyr Ser Met Phe Leu Lys

610 615 620

Leu Leu His Glu Trp Thr Pro Aru Leu Thr Pro Wing Gln Val Wing Glu625 630 635 640

Lys Val Lys Arg Phe Phe Phe Phe Tyr Ala Ile Asn Arg His Lys Gln

645 650 655

Thr Val Leu Thr Pro Ser Tyr His Wing Glu Gln Tyr Ser Pro Asp Asp

660 665 670

Asn Arg Phe Asp Read Arg Pro Phe Read Ile Asn Pro Arg Phe Pro Trp

675 680 685

Ala Ser Lys Lys Ile Asp Glu Val Val Lys Gln Cys Glu Gly His Ser

690 695 700

Ser Glu Ile Asp Phe Met Thr Ile Asp705 710

<210> 14 <211> 714 <212> PRT

<213> Kluyveromyces lactis <400> 14

Met Ser Gln Leu Ile Thr Val Wing Thr Cys Asn Leu Asn Gln Trp Wing 1 5 10 15

Read Asp Phe Glu Gly Asn Arg Asp Arg Ile Phe Glu Ser Ile Lys Ile

20 25 30

Lys Wing Glu Arg Gly Lys Wing Leu Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Thr Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Asp Asp Val Phe Leu

50 55 60

His Ser Trp Glu Met Tyr Gly Gln Ile Ile Lys Arg Pro Glu Thr His65 70 75 80

Gly Ile Leu Read Asp Ile Gly Met Pro Val Met His Arg Asn Val Arg

85 90 95

Tyr Asn Cys Arg Ile Read Be Read Asp Gly Lys Ile Read Phe Ile Arg

100 105 HO

Pro Lys Ile Trp Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Phe

115 120 125

Phe Thr Pro Trp Met Lys Wing Wing His Thr Glu Glu Tyr Leu Leu Pro

130 135 140

Pro Met Ile Gln Lys Read Thr Gly Gln Tyr Arg Ile Pro Phe Gly Asp145 150 155 160

Val Wing Ile Be Thr Read Asp Thr Cys Ile Gly Wing Glu Thr Cys Glu

165 170 175

Glu Read Phe Thr Pro Gln Be Pro His Ile Wing Met Be Read Asp Gly

180 185 190

Val Glu Ile Phe Thr Asn Be Gly Be His His Glu Read Arg Lys

195 200 205

Leu Asp Lys Arg Leu Asp Leu Ile Met Be Wing Thr Arg Arg Cys Gly

210 215 220

Gly Val Tyr Leu Tyr Asn Wing Gln Arg Gly Cys Asp Gly Asp Arg Leu225 230 235 240

Tyr Tyr Asp Gly Cys Wing Read Ile Cys Val Asn Gly Ser Ile Val Wing

245 250 255

Gln Gly Ser Gln Phe Cys Read Lys Asp Val Glu Val Val Thr Wing Thr260 265 270

Val Asp Leu Glu Gln Val Arg Be Tyr Arg Be Thr Val Met Be Arg

275 280 285

Gly Leu Gln Wing Be Read Thr Glu Thr Lys Phe Lys Arg Ile Asp Val

290 295 300

Glu Val Glu Leu Wing Thr Thru Asp Asp Arg Phe Asp Ser Thr Leu Val305 310 315 320

Pro Glu Lys Pro Arg Lys Wing Phe Tyr His Ile Pro Be Glu Glu Ile

325 330 335

Wing Read Gly Pro Wing Cys Trp Read Trp Asp Tyr Read Le Arg Arg Cys Asn

340 345 350

Gly Thr Gly Tyr Phe Gave Pro Read Gly Gly Gly Ile Asp Be Cys Wing

355 360 365

Thr Wing Val Ile Val His Be Met Cys Arg Read Val Val Lys Glu Wing

370 375 380

Glu Wing Gly Asn Gln Gln Val Ile Lys Asp Val Arg Arg Leu Wing Arg385 390 395 400

Met Asn Asp Glu Trp Ile Pro Lys Thr Pro Gln Glu Leu Wing Asn Lys

405 410 415

Ile Phe Asn Thr Cys Phe Met Gly Thr Glu Asn Be Ser Lys Glu Thr

420 425 430

Arg Ser Arg Wing Lys Lys Leu Wing Glu His Ile Gly Wing Tyr His Val

435 440 445

Asp Leu Asn Met Asp Be Leu Val Be Be Met Val Thr Leu Phe Glu

450 455 460

Val Thr Thr Gly Lys Arg Pro Ile Phe Lys Ile Phe Gly Gly Ser Gln465 470 475 480

Thr Glu Asn Leu Wing Leu Gln Asn Ile Gln Arg Wing Leu Arg Met Val

485 490 495

Leu Wing Tyr Leu Phe Wing Gln Leu Leu Pro Trp Val Arg Ser Ile Pro

500 505 510

Asn Wing Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Cys515 520 525

Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys To Be Ala Asp Ile Asn

530 535 540

Pro Ile Gly Gly Ile Ser Lys Thr Asp Read Lys Lys Phe Ile Wing Tyr545 550 555 560

Wing Ser Lys Glu Phe Asp Leu Pro Ile Leu Glu Phe Leu Asn Wing

565 570 575

Thr Pro Thr Glu Wing Leu Glu Pro Ile Thr Lys Asn Tyr Val Gln Ser

580 585 590

Asp Glu Ile Asp Met Gly Met Thr Tyr Glu Glu Read Ser Val Phe Gly

595 600 605

Tyr Leu Arg Lys Val Glu Lys Cys Gly Pro Phe Ser Met Tyr Leu Lys

610 615 620

Leu Leu His Glu Trp Thr Pro Lys Leu Thr Pro Wing Gln Val Wing Glu625 630 635 640

Lys Val Lys Lys Phe Phe Phe Phe Phe Tyr Wing Ile Asn Arg His Lys Gln

645 650 655

Thr Val Leu Thr Pro Ser Tyr His Wing Glu Gln Tyr Ser Pro Asp Asp

660 665 670

Asn Arg Phe Asp Read Arg Pro Phe Read Ile Asn Pro Arg Phe Pro Trp

675 680 685

Phe Lys Wing Ile Asp Asp Wing Val Wing Wing Gln Ser Glu Gly Thr Leu

690 695 700

Ser Gly Wing Read Asp Val Met Thr Val Glu705 710

<210> 15 <211> 715 <212> PRT

<213> Eremothecium gossypii <400> 15

Met Be His Leu Ile Thr Leu Wing Thr Cys Asn Leu Asn Gln Trp Ala1 5 10 15Leu Asp Phe Glu Gly Asn Arg Asp Arg Ile Leu Glu Ser Ile Arg Ile

20 25 30

Wing Lys Glu Lys Asn Wing Lys Leu Arg Val Gly Pro Glu Leu Glu Val

35 40 45

Ser Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Asp Asp Val Tyr Leu

50 55 60

His Ser Trp Glu Met Tyr Wing Gln Ile Read Lys Asp Glu Lys Thr His65 70 75 80

Gly Ile Leu Read Asp Ile Gly Met Pro Val Val His Lys Asn Val Arg

85 90 95

Tyr Asn Cys Arg Val Leu Being Read Asp Gly His Ile Leu Phe Ile Arg

100 105 110

Pro Lys Leu Trp Leu Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Phe

115 120 125

Phe Thr Pro Trp Met Lys Pro Thr Val Glu Glu Phe Gln Leu Pro

130 135 140

Pro Val Ile Gln Lys Ile Thr Gly Gln His Ile Ile Pro Phe Gly Asp145 150 155 160

Val Ile Wing Arg Thr Read Asp Thr Cys Ile Gly Glu Wing Thr Cys Glu

165 170 175

Glu Read Phe Thr Pro Gln Be Pro Asn Ile Wing Met Be Read Asp Gly

180 185 190

Val Glu Ile Ile Thr Asn Be Ser Gly Be His His Glu Leu Arg Lys

195 200 205

Leu His Lys Arg Leu Asp Leu Ile Leu Gly Wing Thr Gly Arg Cys Gly

210 215 220

Gly Val Tyr Leu Tyr Asn Wing Gln Arg Gly Cys Asp Gly Asp Arg Leu225 230 235 240

Tyr Tyr Asp Gly Cys Wing Leu Ile Wing Val Asn Gly Arg Val Val Wing

245 250 255

Gln Gly Be Gln Phe Be Read Arg Asp Val Glu Val Val Thr Wing Thr260 265 270Val Asp Leu Gln Glu Val Arg Asp Tyr Arg Met Val Val Ser Arg

275 280 285

Gly Leu Gln Val Wing Ser Asn Asn Val Thr Phe Glu Arg Ile Gln Val

290 295 300

Pro Val Glu Read Wing Ala Met Gln Asp Arg Phe Asn Pro Thr Ile Asn305 310 315 320

Leu Thr Lys Wing Lys Wing Pro Tyr Tyr His Ser Pro Glu Glu Glu Ile

325 330 335

Wing Read Gly Pro Wing Cys Trp Read Trp Asp Tyr Read Le Arg Arg Cys Arg

340 345 350

Gly Thr Gly Tyr Phe Gave Pro Read Gly Gly Gly Ile Asp Be Cys Wing

355 360 365

Thr Wing Val Ile Val His Be Met Cys Arg Met Val Val Lys Glu Wing

370 375 380

Ser Glu Gly Asn Leu Gln Val Ile Wing Asp Wing Arg Arg Arg Leu Wing Arg385 390 395 400

Wing Be Asp Asp Trp Ile Pro Thr Asp Wing Arg Glu Phe Wing Asn Met

405 410 415

Ile Phe His Thr Cys Phe Met Gly Thr A.la Asn Ser Thr Asn Glu Thr

420 425 430

Arg Ser Arg Wing Lys Lys Leu Wing Glu His Leu Gly Wing Tyr His Val

435 440 445

Asp Leu Asn Met Asp Be Val Val Lys Be Val Val Thr Leu Phe Glu

450 455 460

Val Thr Thr Gly Lys Arg Ile Phe Lys Val Phe Gly Gly Ser Asn465 470 475 480

Ile Glu Asn Leu Wing Wing Leu Gln Asn Ile Glu Wing Arg Leu Arg Met Val

485 490 495

Leu Wing Tyr Leu Phe Wing Gln Leu Leu Pro Trp Val Arg Ser Ile Lys

500 505 510

Asn Ser Gly Gly Leu Leu Val Leu Gly Ser Ala Asn Val Asp Glu Cys515 520 525Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Ser As Wing Asp Ile Asn

530 535 540

Pro Ile Gly Gly Ile Ser Lys Lys Asp Read Lys Asn Phe Ile Ser Tyr545 550 555 560

Wing Ser Lys Glu Phe Asp Leu Pro Ile Leu Arg Glu Phe Val Glu Wing

565 570 575

Thr Pro Thr Glu Wing Leu Glu Pro Ile Thr Glu Asp Tyr Val Gln Ser

580 585 590

Asp Glu Arg Asp Met Gly Met Thr Tyr Glu Glu Read Ser Val Phe Gly

595 600 605

Tyr Leu Arg Lys Val Glu Lys Cys Gly Pro Tyr Ser Met Phe Leu Lys

610 615 620

Leu Leu His Glu Trp Thr Pro Arg Leu Thr Pro Be Glu Val Wing Glu625 630 635 640

Lys Val Lys Arg Phe Phe Phe Tyr Phe Tyr Wing Ile Asn Arg His Lys Gln

645 650 655

Thr Val Leu Thr Pro Ser Tyr His Wing Glu Gln Tyr Ser Pro Asp Asp

660 665 670

Asn Arg Phe Asp Read Arg Pro Phe Read Ile Asp Pro Arg Phe Ser Trp

675 680 685

Ala Ser Lys Lys Ile Asp Leu Val Val Lys Gln Cys Glu Gly Gly Pro

690 695 700

Be Thr Thr Gln Read Asp Val Met Be Val Asp705 710 715

<210> 16 <211> 705 <212> PRT

<213> Yarrowia lipolytica <400> 16

Met Gly His Tyr Val Thr Read Ala Thr Cys Asn Read Asn Gln Trp Ala15 10 15

Read Asp Phe Glu Gly Asn Arg Asp Arg Ile Read Glu As Ile Arg Glu20 25 30

Wing Lys Arg Gln Gly Wing To Be Read Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Thr Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Gly Asp Leu Tyr Leu

50 55 60

His Ser Trp Glu Val Tyr Wing Glu Ile Leu Glu His Pro Asp Thr Ser65 70 75 80

Asp Ile Ile Leu Asp Ile Gly Met Pro Val Met His Lys Asn Val Lys

85 90 95

Tyr Asn Cys Arg Val Ile Ser Tyr Asn Arg Glu Ile Leu Leu Ile Arg

100 105 110

Pro Lys Read Be Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Tyr

115 120 125

Phe Thr Pro Trp Pro Lys Wing Arg Tyr Val Glu Asp Tyr Thr Leu Pro

130 135 140

Arg Phe Val Gln Asn Val Cys Wing Asn Glu Ser Wing Ile Val Pro Phe145 150 155 160

Gly Asp Cys Val Leu Being Thr Lys Asp Wing Val Ile Gly Phe Glu Thr

165 170 175

Cys Glu Glu Leu Phe Thr Pro Gln Being Pro His Ile Gly Met Being Leu

180 185 190

Asp Gly Val Glu Ile Phe Thr Asn Be Ser Gly Be His His Glu Leu

195 200 205

Arg Lys Leu Asn Thr Arg Met Glu Leu Ile Arg Glu Ala Thr Ala Lys

210 215 220

Cys Gly Gly Ile Tyr Read Tyr Wing Asn Gln Arg Gly Cys Asp Gly Asp225 230 235 240

Arg Leu Tyr Tyr Asp Gly Cys Val Wing Ile Val Wing Asn Gly Glu Val

245 250 255

Val Wing Gln Gly Ser Gln Phe Ser Leu Asp Asp Val Glu Val Val Ser

260 265 270

Wing Thr Read Le Asp Leu Glu Wing Val Arg Be Tyr Arg Wing Be Lys Ile275 280 285

Be Gln Cys Met Gln Wing Asn Wing Be Pro Cys Tyr Wing Arg Val Thr

290 295 300

Cys Lys Wing Glu Read To Be Pro To Be Val Thr Phe Asp To Be Glu Val305 310 315 320

Tyr Pro Thr Pro Thr Glu Ile Arg Tyr His Ser Pro Glu Glu Glu

325 330 335

Ile Wing Read Gly Pro Wing Cys Trp Met Trp Asp Tyr Val Arg Arg Cys

340 345 350

Arg Wing Gly Phe Phe Val Pro Wing Read Ser Gly Gly Ile Asp Ser Cys

355 360 365

Wing Thr Wing Wing Ile Val Tyr Ser Met Cys Val Leu Val Wing Asp Wing

370 375 380

Wing Asn Asn Gly Asn Glu Gln Val Ile Lys Asp Wing Arg Val Val Thr385 390 395 400

Gly Asp Pro Asp Phe Val Pro Asp Pro Lys Glu Read Cys Asn Arg

405 410 415

Ile Phe His Thr Cys Phe Met Gly Thr Glu Asn Be Ser Lys Asp Thr

420 425 430

Arg Ser Arg Wing Lys Asp Read Wing Wing Wing Ile Gly Wing Tyr His Thr

435 440 445

Asp Leu Asn Met Asp Ser Val Val Ser Wing Val Arg Gly Leu Phe Glu

450 455 460

Thr Val Thr Gly Lys Arg Ile Phe Lys Val His Gly Gly Ser Ala465 470 475 480

Thr Glu Asn Leu Wing Leu Gln Asn Ile Gln Arg Wing Leu Arg Met Val

485 490 495

Leu Wing Tyr Leu Phe Wing Gln Leu Leu Pro Trp Cys Arg Gly Arg Wing

500 505 510

Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Thr Leu Arg

515 520 525

Gly Tyr Has Read Thr Lys Tyr Asp Cys Ser Be Wing Asp Ile Asn Pro Ile530 535 540

Gly Gly Ile Ser Lys Thr Asp Read Lys Lys Phe Ile Wing Tyr Wing Glu545 550 555 560

His Lys Phe Asp Leu Pro Ile Leu Asn Asp Phe Leu Thr Wing Val Pro

565 570 575

Thr Wing Glu Leu Glu Pro Ile Thr Lys Asp Tyr Val Gln Ser Asp Glu

580 585 590

Val Asp Met Gly Met Thr Tyr Asp Glu Leu Ser Val Phe Gly Arg Leu

595 600 605

Arg Lys Val Glu Lys Cys Gly Pro Tyr Being Met Phe Ile Lys Leu Tyr

610 615 620

His Glu Trp Thr Pro Arg Read Be Wing Glu Gln Ile Wing Wing Lys Val625 630 635 640

Lys Arg Phe Phe Trp Phe Tyr Wing Val Asn Arg His Lys Thr Thr Val

645 650 655

Read Thr Pro Be Tyr His Wing Glu Gln Tyr Be Pro Asp Asp Asn Arg

660 665 670

Phe Asp Leu Arg Pro Phe Leu Ile Asn Pro Gly Phe Ser Trp Ala Ser

675 680 685

Lys Lys Ile Asp Wing Ile Val Lys Being Read Glu Thr Lys Lys Lys Glu690 695 700

Asp705

<210> 17 <211> 714 <212> PRT

<213> Candida albicans <400> 17

Met Gly Asn Tyr Ile Thr Val Ala Thr Cys Asn Leu Asn Gln Trp Ala1 5 10 15

Leu Asp Phe Glu Gly Asn Arg Asp Arg Ile Phe Glu Ser Ile Lys Glu20 25 30Ala Lys Arg Gln Gly Wing Lys Leu Arg Val Gly Pro Glu Leu Glu Val

35 40 45

Cys Gly Tyr Gly Cys Read Asp His Phe Ala Glu Asn Asp Read Tyr Arg

50 55 60

His Ser Trp Glu Val Tyr Gly Glu Ile Read Asn Pro Glu Thr His65 70 75 80

Gly Ile Leu Read Asp Ile Gly Ile Pro Ile Ile His Lys Being Ile Lys

85 90 95

Tyr Asn Cys Arg Ile Ile Be Tyr Asn Gly Lys Ile Leu Read Ile Arg

100 105 110

Pro Lys Ile Tyr Leu Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Tyr

115 120 125

Phe Thr Gly Trp Asn Arg Pro Lys Tyr His Glu

130 135 140

Lys Phe Ile Be Lys Ile Thr Gly Gln Wing Arg Val Pro Phe Gly Asp145 150 155 160

Cys Ile Val Gln Thr Read Glu Thr Arg Read Gly Cys Glu Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Glu Be Pro His Ile Wing Met Wing Leu Asp Gly

180 185 190

Val Glu Ile Phe Thr Asn Be Gly Be His His Glu Read Arg Lys

195 200 205

Leu Asp Thr Arg Leu Lys Leu Ile Thr Glu Wing Thr Lys Lys Cys Gly

210 215 220

Gly Ile Tyr Leu Tyr Asn Wing Gln Lys Gly Cys Asp Gly Asp Arg Leu225 230 235 240

Tyr Tyr Asp Gly Cys Ala Ser Ile Ile Val Asn Gly Asn Val Leu Wing

245 250 255

Gln Wing Be Gln Phe Be Read Lys Asp Val Glu Val Ile Be Wing Thr

260 265 270

Val Asp Read Asp Asp Val Arg Wing Tyr Arg Asn Gln Lys Ser Wing Ser275 280 285Val Gln Wing Val Asn Gln Ser Glu Lys Phe Lys Val Ile Tyr Thr Asp

290 295 300

Val Glu Read To Be Pro Asp Tyr Val Phe Asp To Be Ile Ile Pro305 310 315 320

Be Lys Pro Gln Pro Ile Lys Tyr His Thr Pro Glu Glu Glu Ile Wing

325 330 335

Leu Gly Pro Cys Wing Trp Leu Trp Asp Tyr Leu Arg Arg Be Lys Cys

340 345 350

Gly Gly Tyr Phe Gave Pro Read Gly Gly Gly Ile Asp Be Cys Ala Thr

355 360 365

Val Ile Wing Val His Be Met Cys Arg Leu Val Val Glu Ile Pro Wing

370 375 380

Asp Asp Glu Gln Val Leu Lys Asp Ile Gln Wing Ile Thr His Asp Glu385 390 395 400

Gly Phe Val Lys Thr Pro Gln Asp Ile Wing Gln Arg Ile Phe Tyr

405 410 415

Thr Be Phe Met Gly Thr Be Glu Asn Be Be Lys Glu Thr Be Arg

420 425 430

Ser Lys Glu Leu Wing Ser Lys Ile Gly Ser Tyr His Val Asp Leu Asn

435 440 445

Met Asp Asn Leu Val Thr Be Val Val Be Leu Phe Glu Val Wing Thr

450 455 460

Gly Lys Lys Pro Ile Phe Lys Ile Phe Gly Gly Ser Asn Thr Glu Asn465 470 475 480

Leu Wing Leu Gln Asn Ile Gln Arg Wing Leu Arg Le Met Val Leu Ser Tyr

485 490 495

Leu Phe Ala Gln Leu Leu Pro Trp Thr Arg Gly Lys Asn Val Pro Gly

500 505 510

Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Cys Leu Arg Gly Tyr

515 520 525

Leu Thr Lys Tyr Asp Cys Be Ala Asp Wing Ile Asn Pro Ily Gly Gly530 535 540Ile Be Lys Thr Asp Leu Thr Lys Arg Phe Ile Wing Trp Wing Glu Asn545 550 555 560

Phe Asp Leu Pro Ile Leu His Glu Phe Leu Thr Wing Thr Pro Wing

565 570 575

Glu Leu Glu Pro Ile Thr Lys Asp Tyr Val Gln Ser Asp Glu Ile Asp

580 585 590

Met Gly Met Thr Tyr Asp Glu Read Be Arg Phe Gly Thr Read Le Arg Lys

595 600 605

Val Asp Lys Cys Gly Pro Read Ala Met Phe Ile Lys Read Tyr His Glu

610 615 620

Trp Be Gln Pro Pro Tyr Asn Read Be Wing Lys Gln Ile Wing Glu Lys625 630 635 640

Val Lys Arg Phe Trp Phe Phe Tyr Ala Ile Asn Arg His Lys Met Thr

645 650 655

Thr Met Thr Pro Wing Tyr His Wing Glu Gln Tyr Ser Pro Asp Asp Asn

660 665 670

Arg Phe Asp Read Arg Pro Phe Read Ile Asn Pro Arg Phe Pro His Wing

675 680 685

Ser Lys Lys Ile Asp Glu Leu Val Glu Glu Ile Glu Lys Arg Gln His

690 695 700

Glu Ile Asp Be Ser Asn Lys Ser Val Asp705 710

<210> 18 <211> 716 <212> PRT

<213> Debaryomyces hansenii <400> 18

Met Gly His Tyr Ile Thr Read Ala Thr Cys Asn Leu Asn Gln Trp Ala1 5 10 15

Read Asp Phe Glu Gly Asn Arg Asp Arg Ile Ile Thr Be Ile Ile Glu

20 25 30

Wing Lys Lys Leu Gly Wing Thr Read Le Arg Val Gly Pro Glu Leu Glu Ile35 40 45

Cys Gly Tyr Gly Cys Gave Asp His Phe Gave Glu Asn Asp Gave Tyr Asp

50 55 60

His Ser Trp Glu Met Tyr Gly His Ile Read Thr Asn Pro Asn Thr Gln65 70 75 80

Asp Ile Leu Read Asp Val Gly Met Pro Ile Ile His Lys Ser Ile Lys

85 90 95

Tyr Asn Cys Arg Leu Read Ser Tyr Asn Gly Lys Ile Leu Read Ile Arg

100 105 110

Pro Lys Leu Tyr Leu Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Tyr

115 120 125

Phe Thr Pro Trp Asn Arg Pro Lys Tyr Tyr Glu

130 135 140

Lys Asn Ile To Be Val Thr Gly Gln To Be Val Val Thr Phe Gly Asp145 150 155 160

Cys Val Ile Gln Thr Read Glu Thr Thr Read Gly Wing Glu Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Gln Be Pro His Ile Be Met Wing Read Asp Gly

180 185 190

Val Glu Ile Phe Thr Asn Be Gly Be His His Glu Read Arg Lys

195 200 205

Read Asp Thr Arg Read Le Gln Read Le Ile Thr Gly Wing Thr Lys Lys Cys Gly

210 215 220

Gly Val Tyr Leu Tyr Asn Wing Gln Lys Gly Cys Asp Gly Asp Arg Leu225 230 235 240

Tyr Tyr Asp Gly Cys Wing Cys Ile Ile Val

245 250 255

Gln Wing Be Gln Phe Be Read Arg Asp Val Glu Val Val Be Wing Thr

260 265 270

Ile Asp Read Asp Val Arg Be Tyr Arg Asn Gln Lys Read Be Wing

275 280 285

Phe Gln Be Val Be Gln Be Asp Be Thr Val Tyr His His Ile Pro290 295 300

Thr Asp Ile Glu Read Be Pro Asn Be Asn Val Phe Asn Pro Asn Val305 310 315 320

Lys Pro Be Pro Tyr Arg Asp Ile Arg Tyr His Leu Pro Glu Glu Glu

325 330 335

Ile Wing Leu Gly Pro Wing Cys Trp Leu Trp Asp Tyr Leu Arg Arg Ser

340 345 350

Lys Cys Ally Gly Tyr Phe Leu Pro Leu Ser Gly Gly Ile Asp Ser Cys

355 360 365

Wing Thr Wing Val Ile Val His Leu Met Cys Arg Leu Val Val Lys Ser

370 375 380

Cys Glu Glu Gly Asp Lys Gln Val Ile Ser Asp Ile Gln Ser Leu Thr385 390 395 400

His Asp Pro Glu Phe Val Lys Thr Pro Gln Glu Val Wing Gly Arg

405 410 415

Read Phe Tyr Thr Be Phe Met Gly Thr Glu Asn Be Ser Lys Glu Thr

420 425 430

Arg Be Arg Wing Wing Lys Glu Read Be Glu Lys Val Gly Be His His Ile

435 440 445

Asp Met Asn Met Asp Be Leu Val Be Wing Val Val Be Val Phe Glu

450 455 460

Val Wing Thr Gly Lys Lys Pro Ile Phe Lys Ile Phe Gly Gly Ser Gln465 470 475 480

Thr Glu Asn Leu Wing Leu Gln Asn Ile Gln Arg Wing Leu Arg Met Val

485 490 495

Leu Ser Tyr Leu Phe Ala Gln Leu Leu Pro Trp Thr Arg Asn Ile Ser

500 505 510

Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Cys Leu Arg

515 520 525

Gly Tyr Has Read Thr Lys Tyr Asp Cys Be Ser Asa Asp Ile Asn Pro Ile

530 535 540

Gly Gly Ile Be Lys Thr Asp Read Lys Arg Phe Ile Asp Trp Wing Asp545 550 555 560

Lys Asn Phe Glu Leu Pro Ile Leu His Asp Phe Leu Thr Ala Thr Pro

565 570 575

Thr Wing Glu Leu Glu Pro Ile Thr Gln Asn Tyr Val Gln Ser Asp Glu

580 585 590

Val Asp Met Gly Met Thr Tyr Asp Glu Read Ser Arg Phe Gly Arg Leu

595 600 605

Arg Lys Val Asp Lys Cys Gly Pro Met Wing Met Phe Ile Lys Leu Tyr

610 615 620

His Glu Trp Ser Gln Pro Pro Read Asn Read Thr Wing Glu Gln Val Wing625 630 635 640

Glu Lys Val Lys Arg Phe Trp Phe Phe Tyr Ala Ile Asn Arg His Lys

645 650 655

Met Thr Thr Met Thr Pro Be Tyr His Wing Glu Gln Tyr Be Pro Asp

660 665 670

Asp Asn Arg Phe Asp Asp Read Arg Pro Phe Read Ile Asn Asp Pro Arg Phe Pro

675 680 685

Trp Wing Be Lys Lys Ile Asp Glu Wing Val Asp Ile He Asn Gln Arg

690 695 700

Thr Glu Glu Ile Lys Arg Wing Asn Leu Ser Val Asp705 710 715

<210> 19 <211> 749 <212> PRT

<213> Aspergillus oryzae <400> 19

Met Gly His Leu Val Thr Leu Wing Thr Cys Ser Leu Asn Gln Trp Wing 1 5 10 15

Read Asp Phe Glu Gly Asn Cys Glu Arg Ile Ile Glu Be Ile Arg Gln

20 25 30

Wing Lys Lys Wing Gly Wing Thr Thr Leu Arg Val Gly Pro Glu Leu Glu Ile35 40 45Thr Gly Tyr Gly Val Leu Asp Gly Phe Leu Glu Gly Asp Thr Phe Leu

50 55 60

His Ser Trp Glu Met Leu Wing Arg Ile Ile Asp His Wing Asp Cys Gln65 70 75 80

Asp Ile Val Val Asp Val Gly Met Pro Val Arg His Arg Asn Val Arg

85 90 95

Tyr Asn Cys Arg Val Ile Phe Tyr Asn Arg Lys Ile Ile Read Ile Arg

100 105 110

Pro Lys Met Trp Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Tyr

115 120 125

Phe Thr Pro Trp Gln Arg Pro Gln Ile Glu Asp Tyr Tyr Leu Glu

130 135 140

Ser Ile Val Gly Lys Ile Thr Gly Gln Tyr Lys Val Phe Gly Asp145 150 155 160

Val Ile Wing Be Thr Arg Asp Thr Cys Leu Gly Leu Glu Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Asn Gly Tyr Wing Leu Gln Leu Arg Asn Cys Asp

180 185 190

Tyr His Wing Asn Ile Tyr Val Gly Leu Ile Phe Leu Met Val Leu Pro

195 200 205

Val Trp Pro Be Read Gln Asp Arg Pro Read Thr Be Pro Ile Gly Val

210 215 220

Glu Ile Ile Be Asn Be Gly Be His His Glu Leu Arg Lys Leu225 230 235 240

Asp Thr Arg Ile Asn Read Val Val Gln Wing Thr Lys Read Ser Gly Gly

245 250 255

Ile Tyr Leu Tyr Asn Wing Gln Gln Gly Cys Asp Gly Asp Arg Leu Tyr

260 265 270

Tyr Asp Gly Cys Wing Met Ile Val Val Asn Gly Asn Ile Val Vala Gln

275 280 285

Gly Be Gln Phe Be Read Asn Asp Val Glu Val Val Thr Thr Wing Val290 295 300Asp Ile Glu Glu Val Arg Thr Tyr Arg Be Ser Wing Be Arg Gly Met305 310 315 320

Gln Wing Ser Lys Gln Thr Pro Phe Val Arg Read Asp Read Asp Met Arg

325 330 335

Leu Ser Arg Gln Asn Glu Glu Wing Asp Pro Gly

340 345 350

Wing Ile Wing Pro Arg Tyr His Wing Pro Glu Glu Glu Val Wing Leu Gly

355 360 365

Pro Wing Cys Trp Read Trp Asp Tyr Read Arg Arg Be Gly Wing Ala Gly

370 375 380

Phe Phe Leu Pro Leu To Be Gly Gly Ile Asp To Be Cys Wing Thr Wing Ile385 390 395 400

Ile Val His Be Met Cys Arg Glu Val Ile Lys Wing Val Ser Glu Gly

405 410 415

Glu Asn Glu Val Ile Lys Asp Val Arg Arg Read Cys Wing Glu Pro Wing

420 425 430

Asp Be Thr Trp Leu Pro Thr Thr Be Gln Glu Val Cys Asn Arg Ile

435 440 445

Phe His Thr Be Tyr Met Gly Thr Gln Asn Be Ser Lys Glu Thr Arg

450 455 460

Asp Arg Be Lys Arg Read Be Thr Asp Ile Gly Be Tyr His Val Asp465 470 475 480

Phe Asn Phe Asp Thr Val Val Thr Be Read Thr Asn Read Phe Thr Met

485 490 495

Val Thr Asn Phe Gln Pro Lys Phe Lys Val His Gly Gly Ser Arg Wing

500 505 510

Glu Asn Gln Wing Leu Gln Asn Val Gln Wing Arg Leu Arg Met Val Leu

515 520 525

Be Tyr Leu Phe Ala Be Read Leu Pro Thr Val Arg Gln Arg Pro Gly

530 535 540

Gly Gly Gly Leu Leu Val Leu Wing Be Ser Asn Val Asp Glu Wing Cys545 550 555 560Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Wing Ser Be Asp Wing Leu Asn

565 570 575

Pro Ile Gly Ser Ile Ser Lys Val Asp Read Lys Lys Phe Ile Wing Trp

580 585 590

Be Arg Asp Be Phe Glu Leu Pro Ile Read His Glu Phe Leu Asn Wing

595 600 605

Thr Pro Thr Wing Glu Leu Glu Pro Ile Thr Be Thr Tyr Val Gln Ser

610 615 620

Asp Glu Wing Asp Met Gly Val Thr Tyr Glu Wing Read Be Thr Phe Gly625 630 635 640

Tyr Leu Arg Lys Ile Wing Lys Leu Gly Pro Trp Ser Met Tyr Glu Arg

645 650 655

Leu Leu His Val Trp Gly Asn Glu Tyr Ser Pro Arg Glu Ile Tyr Glu

660 665 670

Lys Thr Arg His Phe Phe Tyr Asn Tyr Ala Ile Asn Arg His Lys Met

675 680 685

Thr Val Leu Thr Pro Ser Tyr His Wing Glu Gln Tyr Ser Pro Asp Asp

690 695 700

Asn Arg His Asp Read Arg Gln Phe Read Tyr Pro Be Phe Thr Trp Ala705 710 715 720

Tyr Lys Lys Met Glu Asp Be Val Lys Tyr Trp Glu Be Lys Gly Trp

725 730 735

Thr Wing Gly Lys Wing Gln Lys Lys Asn Val Lys Wing Asp740 745

<210> 20 <211> 767 <212> PRT

<213> Ustilago maydis <400> 20

Met Gly Leu Pro Val Thr Val Ser Thr Cys Ser Leu Asn Gln Trp Ala15 10 15

Leu Asp Phe Asp Gly Asn Arg Asp Arg Ile Leu Glu Ser Ile Arg Leu20 25 30

Wing Lys Ser Val Gly Ser Arg Leu Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Pro Gly Tyr Gly Cys Phe Asp His Phe Leu Glu Pro Asp Thr Val Leu

50 55 60

His Ser Trp Gln Val Leu Wing Glu Ile Leu Being Ser Asp Wing Thr Asn65 70 75 80

Gly Ile Leu Cys Asp Val Gly Met Pro Val Leu His Arg Be Thr Leu

85 90 95

Tyr Asn Cys Arg Val Leu Leu Leu Asp Gly Lys Ile Leu His Ile Arg

100 105 110

Pro Lys Met Trp Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg Tyr

115 120 125

Phe Ser Pro Trp Thr Thr Asn His Thr Asp Ser Phe Pro Leu Pro

130 135 '140

Arg Ile Val Ser Ile Thr Asp Gln His Glu Val Pro Phe Gly Asp145 150 155 160

Wing Val Val Lys Thr Arg Asp Thr Val Leu Gly Val Glu Leu Cys Glu

165 170 175

Glu Read Phe Thr Pro Asn Be His Ile Arg Pro Gln Gly Read Asp Gly

180 185 190

Val Glu Ile Phe Thr Asn Be Be Wing Be His His Glu Read Arg Lys

195 200 205

Leu Tyr Arg Arg Val Glu Leu Ile Lys Glu Wing Thr Leu Lys Leu Gly

210 215 220

Gly Ile Tyr Leu Tyr Wing Asn Gln Gln Gly Cys Asp Gly Asp Arg Leu225 230 235 - 240

Tyr Tyr Asp Gly Cys Pro Read Ile Val Wing Asn Gly Ser Ile Val Wing

245 250 255

Gln Gly Be Gln Phe Be Read Asp Asp Val Gln Val Val Be Wing Thr

260 265 270

Val Asp Read Asp Asp Val Arg Wing His Arg Be Wing Lys Be Arg Gly275 280 285

Met Gln Wing Val Be His Be Read Gly Be Gly Tyr Pro Arg Ile His

290 295 300

Val Asp Phe Glu Val Gly Glu Be Glu Glu Tyr Be Lys Thr Pro305 310 315 320

Gly Thr Be Pro Val Val Wing Gly Thr Be Pro Val Val Wing Asp

325 330 335

Gly Gln Arg Asp Asp Glu Arg Wing Read Tyr Lys Arg Tyr Read Thr Thr

340 345 350

Read His Gln Pro Ile Glu Val His Tyr His Gln Pro Gln Glu Ile

355 360 365

Wing Read Gly Pro Wing Cys Trp Read Trp Asp Tyr Read Le Arg Arg Be Arg

370 375 380

Thr Gln Gly Tyr Phe Val Pro Read Gly Gly Ile Asp As Cys Ala385 390 395 400

Thr Wing Thr Ile Val Phe Be Met Cys Arg Leu Val Ile Wing Ile Wing

405 410 415

Asp Ala Pro Be Ser Be Ser Pro Ala Be Lys Ala Thr Be Ser Leu

420 425 430

Thr Thr Thr Asp Thr Thr Thr Gln Val Leu Gln Asp Val Arg Arg Ile Cys

435 440 445

Asn Glu Lys Pro To Be Thr Thr Ile Pro Wing To Be Pro Gln Glu Leu

450 455 460

Cys Asn Arg Ile Phe Val Thr Cys Tyr Met Gly Thr Glu Asn Ser465 470 475 480

Glu Wing Thr Arg Gln Arg Wing Lys Asp Read Wing Wing Asp Ile Gly Wing

485 490 495

Tyr His Ile Asp Leu Asn Met Asp Ile Val Val Arg Wing Ile Ile Wing

500 505 510

Read Phe Be Thr Val Thr Gly Be Thr Pro Arg Thr Phe Arg Val His Gly

515 520 525

Gly Thr Pro Glu Wing Asn Leu Wing Leu Gln Asn Xle Gln Wing Airg Leu530 535 540

Arg Met Read Leu Wing Tyr Met Phe Wing Gln Leu Thr Pro Trp Val Arg545 550 555 560

Gly Ser Trp Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu

565 570 575

Ser Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Ser Ala Asp Ile

580 585 590

Asn Pro Ile Gly Gly Ile Ser Lys Thr Asp Read Lys Ala Phe lie. Allah

595 600 605

Tyr Ala Arg Asp Ala Phe Ser Leu Pro Ile Leu His Ser Phe Leu Thr

610 615 620

Val Wing Pro Thr Glu Wing Leu Glu Pro Ile Thr Glu Ser Tyr Val Gln625 630 635 640

Asp Glu Wing Asp Met Gly Wing Thr Tyr Asp Glu Read Ser Val Phe

645 650 655

Gly Arg Read Lys Asn Arg Lys Read Cys Lys Gly Pro Tyr Be Met Phe Asn

660 665 670

Lys Leu Read Le Gln Gln Trp Gly Pro Thr Met Gly Pro Glu Arg Val Wing

675 680 685

Glu Lys Val Lys Leu Phe Trp Phe Glu Tyr Wing Arg Asn Arg His Lys

690 695 700

Met Thr Thr Read Thr Pro Be Tyr His Wing Glu Be Tyr Be Pro Asp705 710 715 720

Asp Asn Arg Phe Asp Asp Read Arg Pro Phe Read Tyr Pro Be Arg Phe Pro

725 730 735

Phe Gln Phe Arg Lys Ile Asp Glu Read Val Lys Arg Read Gln Wing Phe

740 745 750

Gln Wing Ile Pro Pro Pro Be Arg Asp Glu Asn Arg Lys Gln Val

755 760 765

<210> 21 <211> 706 <212> PRT <213> Cryptococcus neoformans <400> 21

Met His Leu Val Thr Val Wing Thr Cys Gln Leu Arg Gln Trp Ser Leu1 5 10 15

Asp Phe Glu Gly Asn Cys Glu Arg Ile Read Arg Be Ile Wing Ile Wing

20 25 30

Lys Ser Arg Gly Ala Thr Read Le Arg Val Gly Pro Glu Leu Glu Val Pro

35 40 45

Gly Tyr Gly Cys Asp Thr Met Leu His Ser Trp Glu Val Leu Wing Lys

50 55 60

Ile Leu Gln Be Glu Glu Wing Lys Gly Ile Val Cys Asp Ile Gly Met65 70 75 80

Pro Read Glu His Lys Asn Asn Asn Tyr Asn Cys Arg Val Ile Ile Phe

85 90 95

Asn Gly Lys Ile Leu Leu Ile Arg Pro Lys Met Trp Met Wing Asn Asp

100 105 110

Gly Asn Tyr Arg Glu Read His Arg Phe Thr Pro Trp His Lys His Arg

115 120 125

Gln Val Glu Lys His Being Read To His Met Ile Arg Ile Val Thr Gly

130 135 140

Gln Thr Tyr Val Pro Phe Gly Asp Wing Val Ile Thr Wing Glu Asp Thr145 150 155 160

Val Ile Gly Val Glu Read Cys Glu Glu Read Phe Thr Pro Wing Ser Pro

165 170 175

His Ile Leu Met Gly Leu Asp Gly Val Glu Ile Phe Thr Asn Ser

180 185 190

Gly Ser His His Glu Leu Arg Lys Leu Asn Arg Arg Val Glu Leu Ile

195 200 205

Lys Glu Wing Thr Met Lys Leu Gly Gly Ile Tyr Leu Tyr Ala Asn Gln

210 215 220

Gln Gly Cys Asp Gly Asp Arg Read Tyr Tyr Asp Gly Cys Wing Read Ile225 230 235 240Ala Met Asn Gly Gln Ile Read Wing Gln Gly Pro Gln Phe Ser Read Le Ser

245 250 255

Glu Val Glu Val Val Ser Wing Thr Val Asp Leu Arg Wing Val Arg Wing

260 265 270

His Arg Thr Thr Be Be Arg Arg Met Gln Be Wing Gln Wing Glu Wing

275 280 285

Tyr Glu Arg Val Val Wing Asp Thr Arg Read Asp Gly Gly Glu Gln Ile

290 295 300

Lys Val Gly Leu Arg Glu Thr Lys Gly Be Met Asp Val Arg Tyr His305 310 315 320

Thr Pro Glu Glu Glu Ile Wing Leu Gly Pro Wing Cys Trp Leu Trp Asp

325 330 335

Tyr Leu Arg Arg Be Arg Thr Gln Gly Tyr Phe Leu Pro Read Be Gly

340 345 350

Gly Ile Asp Be Cys Wing Thr Wing Ile Ile Val His Be Met Cys Arg

355 360 365

Leu Val Val Glu Wing Wing Wing Lys Gly Asp Glu Gln Val Ile Thr Asp

370 375 380

Wing Arg Arg Ile Thr Asn Glu Pro Glu Asp Be Thr Tyr Ile Pro Glu385 390 395 400

Asp Pro Arg Glu Phe Gly Wing Arg Ile Phe His Thr Cys Tyr Met Gly

405 410 415

Thr Glu Asn Be Be Be Glu Thr Arg Glu Arg Wing Alys Lys Asn Leu Wing

420 425 430

Asp Wing Ile Gly Wing Tyr His Val Asp Read Asn Met Asp Thr Wing Wing

435 440 445

Be Val Val Gly Ile Phe Be Val Val Gly Lys Thr Pro Gln

450 455 460

Phe Lys Wing His Gly Gly Thr Asn Wing Glu Asn Leu Wing Wing Leu Gln Asn465 470 475 480

Ile Gln Arg Wing Arg Leu Arg Met Val Val Ser Tyr Met Phe Gln Wing Leu485 490 495Leu Pro Trp Val Arg Gly Lys Asn Gly Gly Leu Leu Val Leu Gly Ser

500 505 510

Wing Asn Val Asp Glu Being Read Arg Gly Tyr Phe Thr Lys Tyr Asp Cys

515 520 525

Be Ser Asa Val Val Asn Pro Ile Gly Gly Ile Ser As Val Val Asp Leu

530 535 540

Lys Arg Phe Ile Trp Wing Gln Val Val Lys Phe Asp Leu Pro Ile Leu545 550 555 560

Tyr Asn Phe Leu His Wing Val Pro Thr Wing Glu Leu Ile Pro Ile Gly

565 570 575

Pro Asp Asn Ile Ile Gln Be Asp Ile Glu Met Gly Met Thr Tyr

580 585 590

Asp Glu Leu Be Val Tyr Gly Arg Leu Arg Lys Val Glu Lys Cys Gly

595 600 605

Pro Phe Be Met Phe Gly Lys Leu Val Gln Glu Trp Gly Be Phe Leu

610 615 620

Ser Pro Lys Glu Ile Wing Glu Lys Val Lys His Phe Phe Phe Met Tyr625 630 635 640

Alle Ile Asn Arg His Lys Met Thr Thr Ile Thr Pro Be Val His Met

645 650 655

Glu Ser Tyr Ser Pro Asp Asp Asn Arg Phe Asp Leu Arg Pro Phe Leu

660 665 670

Tyr Pro Being Gln Phe Thr His Gln Phe Arg Lys Ile Asp Glu Leu Wing

675 680 685

Gly Lys Leu Pro Asp Met Wing Gln Lys Pro Lys Val Asp Thr Asn Glu

690 695 700

Val Asp705 <210> 22 <211> 700 <212> PRT

<213> Schizosaccharomyces pombe <400> 22

Met Glu Arg Tyr Val Thr Ile Wing Be Cys Gln Read Asn Gln Trp Wing 1 5 10 15

Met Asp Phe Glu Gly Asn Arg Read Le Arg Ile Ile Asp Be Ile Lys Glu

20 25 30

Wing Lys Arg Gln Asn Wing To Be Read Arg Val Gly Pro Glu Leu Glu Val

35 40 45

Gly Tyr Thr Gly Cys Glu Asp His Phe Read Glu Ser Asp Thr Tyr Tyr

50 55 60

His Ser Trp Glu Met Leu Cys Ser Ile Ile His Asp Pro Asp Cys Gln65 70 75 80

Asp Ile Leu Read Asp Ile Gly Met Pro Val Met His Lys Wing Met Arg

85 90 95

His Asn Cys Arg Ile Leu Ala Leu Asn Gly Lys Ile Leu Leu Ile Arg

100 105 110

Pro Lys Ile Trp Read Cys Asp Asp Gly Asn Phe Arg Glu Be Arg Trp

115 120 125

Phe Thr Pro Trp Leu Arg Pro Arg Val Glu Thr His Tyr Leu Pro

130 135 140

Thr Phe Val Wing Lys Ser Asu Asn Gln Thr Val Pro Ile Gly Asp145 150 155 160

Wing Ile Read Gln Cys Asn Glu Thr Val Gly Val Glu Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Asn Ser Pro His Ile Asp Met Wing Read Asp Gly

180 185 190

Val Glu Ile Phe Ile Asn Wing Be Gly Be His His Glu Read Arg Lys

195 200 205

Leu Thr Thr Arg Val Asn Leu Ile Gln Asn Wing Thr Glu Lys Cys Gly

210 215 220

Gly Ile Tyr Leu Tyr Ser Asn Gln Arg Gly Cys Asp Gly Gly Arg Leu225 230 235 240

Tyr Tyr Asp Gly Be Ser Met Ile Phe Ala Asn Gly Lys Met Leu Ala245 250 255

Gln Gly His Gln Phe Be Read Lys Asp Val Glu Val Ile Be Wing Thr

260 265 270

Val Asp Val Asp Thr Val Arg Be Tyr Arg Phe Gln Pro Be His Gly

275 280 285

Glle Ily Gly Val Thr Arg Pro Be Tyr Glu Arg Ile His Val Asn Phe

290 295 300

Be Read Be Ser Tyr Gln Gln Asp Tyr Asp Ile Tyr Arg Lys Pro Thr305 310 315 320

Asp Pro Ile Glu Val Thr Ile Pro Leu Pro Glu Glu Glu Ile Thr Phe

325 330 335

Gly Pro Wing Cys Trp Read Trp Asp Tyr Read Arg Arg Be His Wing Ala

340 345 350

Gly Phe Phe Leu Pro Leu Be Gly Gly Phe Leu Asp Be Cys Be Thr Wing

355 360 365

Val Leu Val Tyr Be Met Cys Arg Ile Val Cys Lys Wing Met Glu Glu

370 375 380

Asp Asp Wing Gln Val Leu Ser Asp Val Arg Arg Ile Val Gly Asp Pro385 390 395 400

Ser Tyr Ser Ser Thr Asp Pro Lys Lys Leu Leu Asn His Leu Phe Tyr

405 410 415

Thr Wing Phe Met Gly Be Glu His Be Be Lys Glu Thr Arg Be Arg

420 425 430

Ala Lys Glu Read Be Ser Read Ile Gly Be Tyr His Thr Asp Val Asn

435 440 445

Ile Asp Thr Met Thr Be Val Wing Val Lys Leu Phe Wing Leu Val Thr

450 455 460

Gly Lys Thr Pro Gln Phe Arg Ser Asn Gly Lys Thr Asn Wing Glu Asn465 470 475 480

Leu Wing Leu Gln Asn Ile Gln Wing Arg Be Arg Met Leu Leu Gly Tyr

485 490 495

Leu Phe Ala Gln Leu Leu Pro Trp Val Arg Gly Tyr Ser Gly Ser Leu500 505 510

Leu Val Leu Gly Be Ser Asn Val Asp Glu Cys Leu Arg Gly Tyr Leu

515 520 525

Thr Lys Tyr Asp Cys Being Ser Asa Asp Ile Asn Pro Ile Gly Gly Ile

530 535 540

Ser Lys Thr Asp Leu Lys Ser Phe Leu Arg Tyr Wing Lys Glu Wing Leu545 550 555 560

Asp Leu Pro Ile Leu Gln Glu Phe Leu Asp Wing Thr Pro Wing Wing Glu

565 570 575

Leu Glu Pro Thr Thr Glu Be Tyr Val Gln Be Asp Glu Wing Asp Met

580 585 590

Gly Met Thr Tyr Wing Glu Read Ser Val Phe Gly Arg Read Le Arg Lys Ile

595 600 605

Being Lys Cys Gly Pro Tyr Being Met Phe Thr Gln Read Met His Gln Trp

610 615 620

Gly Asp Arg Read Be Pro Be Gln Val Wing Glu Lys Val Lys Arg Phe625 630 635 640

Phe His Tyr Tyr Gly Ile Asn Arg His Lys Met Thr Thr

645 650 655

Ser Tyr His Wing Glu Thr Tyr Gly Val Asp Asp Asn Arg Tyr Asp Leu

660 665 670

Arg Gln Phe Read Tyr Pro Be Trp Thr Trp Gln Asn Lys Lys Ile Asp

675 680 685

Wing Read Wing Be Lys Phe Glu Gln His Gln Arg Lys690 695 700

<210> 23 <211> 678 <212> PRT

<213> Aspergillus nidulans <400> 23

Met Gly Arg Leu Ile Thr Leu Wing Thr Cys Ser Leu Asn Gln Trp Ala15 10 15Leu Asp Trp Glu Gly Asn Cys Glu Arg Ile Ile Glu Ser Ile Arg Gln

20 25 30

Wing Lys Wing Wing Gly Wing Thr Thr Leu Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Thr Gly Tyr Gly Val Leu Asp Gly Phe Leu Glu Gly Asp Thr Phe Leu

50 55 60

His Ser Trp Glu Met Leu Wing Arg Ile Ile Asp His Pro Asp Cys Gln65 70 75 80

Asp Ile Val Val Asp Val Asp Gly Asn Tyr Arg Glu Met Arg His Phe

85 90 95

Thr Pro Trp Gln Arg Pro Arg Glu Val Glu Asp Tyr Tyr Leu Glu Gln

100 105 110

Ile Val Gly Lys Ile Thr Gly Gln Tyr Lys Val Phe Gly Asp Wing

115 120 125

Val Ile Ser Thr Arg Asp Thr Cys Leu Gly Leu Glu Thr Cys Glu Glu

130 135 140

Read Phe Thr Pro Asn Gly Pro His Ile Pro Tyr Ser Read Ala Gly Wing Val145 150 155 160

Glu Ile Ile Be Asn Be Gly Be His His Glu Leu Lys Lys Leu

165 170 175

Asp Thr Arg Val Asn Leu Ile Thr Gln Wing Thr Lys Leu Ser Gly Gly

180 185 190.

Ile Tyr Leu Tyr Asn Wing Gln Gln Gly Cys Asp Gly Asp Arg Leu Tyr

195 200 205

Tyr Asp Gly Cys Ala Met Ile Val Ile Asn Gly Asn Ile Val Ala Gln

210 215 220

Gly Ser Gln Phe Ser Leu Asn Asp Val Glu Val Val Thr Wing Val225 230 235 240

Asp Ile Glu Glu Val Arg Thr Tyr Arg Wing Be Thr Be Arg Asn Met

245 250 255

Gln Ala Ser Arg Gln Pro Pro Phe Val Arg Read Asp Read Asp Thr Arg260 265 270Leu Be Arg Be Asp Glu Asp Wing Asp Pro Gly Ile Wing Pro Be Glu

275 280 285

Thr Leu Ile Pro Arg Tyr His Wing Pro Glu Glu Glu Ile Wing Leu Gly

290 295 300

Pro Wing Cys Trp Leu Trp Asp Tyr Leu Arg Arg Be Gly Wing Ala Gly305 310 315 320

Phe Phe Leu Pro Leu To Be Gly Gly Ile Asp To Be Cys Wing Thr Wing Wing

325 330 335

Ile Val His Be Met Cys Arg Glu Val Ile Lys Wing Val Gln Gln Gly

340 345 350

Glu Asn Glu Val Ile Lys Asp Val Arg Arg Read Cys Wing Glu Pro Wing

355 360 365

Gly Be Thr Trp Read Pro Thr Thr Be Gln Glu Val Cys Asn Phe Met

370 375 380

Gly Thr Gln Asn Be Ser Lys Glu Thr Arg Asp Arg Wing Lys Glu Leu385 390 395 400

Wing Glu Wing Ile Gly Ser Tyr His Ile Asp Phe Asn Phe Asp Thr Val

405 410 415

Val Thr Wing Read Met Asn Leu Phe Thr Val Val Thr Asn Phe Gln Pro

420 425 430

Arg Phe Lys Val His Gly Gly Ser Arg Wing Glu Asn Gln Wing Leu Gln

435 440 445

Asn Ile Gln Arg Wing Arg Le Arg Met Val Leu Ser Tyr Leu Phe Ala Ser

450 455 460

Leu Leu Pro Thr Val Arg Gln Arg Pro Gly Gly Gly Gly Leu Leu Val465 470 475 480

Leu Wing Be Ser Asn Val Asp Glu Cys Leu Arg Gly Tyr Leu Thr Lys

485 490 495

Tyr Asp Ala Ser Be Ala Asp Read Asn Pro Ile Gly Be Ile Be Lys

500 505 510

Val Asp Leu Lys Lys Phe Ile Gly His Cys Wing Thr Be Phe Asp Met515 520 525Pro Ile Leu Thr Be Phe Leu Asn Wing Thr Pro Thr Wing Glu Leu Glu

530 535 540

Pro Ile Thr Wing Thr Tyr Val Gln Ser Asp Glu Wing Asp Met Gly Val545 550 555 560

Thr Tyr Wing Glu Leu Gly Thr Phe Gly Tyr Leu Arg Lys Val Ser Lys

565 570 575

Read Gly Pro Trp Be Met Tyr Glu Arg Read Leu His Met Trp Gly Asn

580 585 590

Glu Tyr Be Pro Arg Glu Ile Tyr Glu Lys Thr Arg His Phe Phe Tyr

595 600 605

Asn Tyr Ala Ile Asn Arg His Lys Met Thr Val Ile Thr Pro Be Tyr

610 615 620

His Wing Glu Gln Tyr Be Pro Asp Asp Asn Arg His Asp Read Arg Gln625 630 635 640

Phe Leu Tyr Pro Pro Phe Thr Trp Wing Tyr Lys Lys Met Glu Glu Ser

645 650 655

Val Lys Tyr Trp Glu Glu Arg Gly Trp Thr Thr Gly Lys Wing Gln Lys

660 665 670

Lys Ser Val Lys Wing Asp

675 <210> 24 <211> 729 <212> PRT

<213> Neurospora crassa <400> 24

Met Gly Gly His Leu Val Thr Val Wing Thr Cys Ser Leu Asn Gln Trp1 5 10 15

Val Leu Asp Trp Glu Gly Asn Leu Gln Arg Ile Val Glu Ser Ile His

20 25 30

Leu Wing Lys Lys Wing Gly Arg Wing Arg Read Le Arg Val Gly Pro Glu Leu Glu

35 40 45

Ile Cys Gly Tyr Being Asp Read Asp His Phe His Glu Read Asp Val Tyr50 55 60

Thr His Ser Leu Glu Met Leu Arg Lys Leu Leu Glu Asp Glu Ser Cys65 70 75 80

His Asp Ile Leu Ile Asp Val Gly Leu Pro Ile Read His Arg Asn Ile

85 90 95

Arg Tyr Asn Wing Arg Wing Ile Leu Leu Asn Gly Lys Ile Leu Leu Ile

100 105 110

Arg Pro Lys Met Trp Read Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg

115 120 125

His Phe Thr Pro Trp Met Arg Pro Arg Glu Thr Glu Leu Phe His Leu

130 135 140

Pro Lys Ile Leu Gln Glu Ile Gln Gly Glu Thr His Val Leu Phe Gly145 150 155 160

Asp Wing Val Ile Be Thr Pro Glu Thr Wing Phe Gly Wing Glu Thr Cys

165 170 175

Glu Glu Leu Phe Thr Pro Lys Wing Pro His Ile Asp Met Wing Leu Asp

180 185 190

Gly Val Glu Ile Ile Thr Asn Be Ser Gly Be His Phe Thr Leu Gln

195 200 205

Lys Leu Asp Val Arg Leu Gln Leu Ile Met Glu Wing Thr Arg Lys Ser

210 215 220

Gly Gly Val Tyr Read Tyr Wing Asn Gln Gln Gly Cys Asp Gly Glu Arg225 230 235 240

Tyr Phe Asp Gly Cys Ala Met Ile Ile Val Asn Gly Asn Ile Val

245 250 255

Gln Wing Gly Be Gln Phe Be Read Asn Asp Val Glu Val Val Thr Wing

260 265 270

Thr Val Asp Leu Glu Glu Val Arg Wing Tyr. Arg Ser Ser Ile Ser Arg

275 280 285

Gly Leu Gln Wing Wing Thr Be Asn Wing Lys Tyr Gln Arg Ile Gln Thr

290 295 300

Be Phe Glu Leu Be Pro Glu Asp Glu Asp Thr Asp Ile Trp Lys Lys305 310 315 320

Pro Thr Read Pro Arg Pro Pro Arg Tyr His Ser Val Glu Glu Glu Ile

325 330 335

Wing Read Cys Gly Gly Tys Cyr Tyr Leu Trp Asp Tyr Leu Arg Arg Be Gly

340 345 350

Thr Wing Gly Tyr Leu Val Pro Leu To Be Gly Gly Ile Asp To Be Cys Wing

355 360 365

Thr Wing Thr Read Le Val Phe Be Met Cys Arg Ile Val Ile Gln Wing Ile

370 375 380

Glu Asp Gly Asn Gln Gln Val Ile Asp Val Asp Val Cys Ile Cys Lys385 390 395 400

Tyr Gly Lys Glu Gly Glu Leu Pro Lys Thr Pro Gln Glu Leu Cys Asn

405 410 415

Gln Val Phe Thr Thr Ile Tyr Met Gly Met Being Lys Gln Being Being Wing

420 425 430

Glu Thr Arg Gly Arg Wing Lys Glu Read Asp Wing Ile Gly Be Tyr

435 440 445

His Val Asn Leu Asp Ile Asp Asp Val Tyr Glu Wing Gln Lys Lys Leu

450 455 460

Ile Val Gln Thr Thr Asn Phe Glu Pro Arg Phe Lys Val His Gly Gly465 470 475 480

Thr Val Gln Glu Asn Leu Thr Read Gln Cys Leu Gln Wing Arg Ile Arg

485 490 495

Met Val Thr Wing Tyr Glu Phe Gly Gln Ile Leu Pro Thr Wing Arg Gly

500 505 510

Arg Pro Gly Gly Gly Ser Leu Leu Val Leu Gly Ser Wing Asn Val Gly

515 520 525

Glu To Be Read Arg Gly Tyr To Read Thr Lys Tyr Asp Cys To Be Ala Asp

530 535 540

Ile Asn Pro Ile Gly Ser Ile Asp Lys Wing Asp Leu Lys Arg Phe Ile545 550 555 560

Trp Wing Glu Wing Lys Lys Phe Asp Leu Pro Cys Leu His Gly Phe Leu565 570 575

Thr Wing Val Pro Thr Wing Glu Leu Glu Pro Ile Thr Gln Glu Tyr Val

580 585 590

Gln Ser Asp Glu Wing Asp Met Gly Met Thr Tyr Wing Glu Leu Thr Val

595 600 605

Phe Gly Arg Leu Arg Lys Leu Asn Lys Leu Gly Pro Tyr Ala Met Phe

610 615 620

Gln Arg Read Val His Asp Trp Be Wing Asp Arg Glu Lys Val Glu Gly625 630 635 640

Asp Glu Pro Wing Phe Tyr Thr Pro Arg Gln Val Wing Glu Lys Val Lys

645 650 655

Arg Phe Phe His Phe Tyr Ala Ile Asn Arg His Lys Met Thr Thr Read

660 665 670

Thr Pro Wing Read His Cys Asn Asp Tyr Be Pro Asp Asp Asn Arg Phe

675 680 685

Asp Leu Arg Pro Phe Leu Tyr Pro Pro Phe Trp Lys Ser Trp Ser Phe

690 695 700

Lys Arg Ile Asp Met Glu Read Glu Arg Ile Glu Lys Lys Arg Glu Glu

ι

705 710 715 720

Arg Wing Gly Lys Gly Lys Glu Thr Ala725

<210> 25 <211> 652 <212> PRT

<213> Cryptococcus neoformans <400> 25

Met Leu His Ser Trp Glu Val Leu Wing Lys Ile Leu Gln Ser Glu Glu1 5 10 15

Lys Wing Gly Ile Val Cys Asp Ile Gly Met Pro Read Glu His Lys Asn

20 25 30

Asn Asn Tyr Asn Cys Arg Val Ile Ile Phe Asn Gly Lys Ile Leu Leu35 40 45Ile Arg Pro Lys Met Trp Met Wing Asn Asp Gly Asn Tyr Arg Glu Leu

50 55 60

Arg His Phe Thr Pro Trp His Lys His Arg Gln Val Glu Lys His Ser65 70 75 80

Read His Pro Met Ile Arg Ile Val Thr Gly Gln Thr Tyr Val Pro Phe

85 90 95

Gly Asp Val Val Wing Ile Thr Glu Asp Val Val Wing Gly Val Glu Leu

100 105 110

Cys Glu Glu Leu Phe Thr Pro Wing Ser Pro His Ile Leu Met Gly Leu

115 120 125

Asp Gly Val Glu Ile Phe Thr Asn Be Ser Gly Be His His Glu Leu

130 135 140

Arg Lys Leu Asn Arg Arg Val Glu Leu Ile Lys Glu Wing Thr Met Lys145 150 155 160

Read Gly Gly Ile Tyr Read Tyr Wing Asn Gln Gln Gly Cys Asp Gly Asp

165 170 175

Arg Read Tyr Tyr Asp Gly Cys Wing Read Ile Wing Met Asn Gly Gln Ile

180 185 190

Read Glu Wing Gly Pro Gln Phe Ser Read Glu Val Glu Val Val Ser

195 200 205

Wing Thr Val Asp Leu Arg Wing Wing Val Arg Wing His Arg Thr Thr Be

210 215 220

Arg Arg Met Gln Be Wing Gln Wing Glu Wing Tyr Glu Arg Val Val Val Wing225 230 235 240

Asp Thr Arg Leu Asp Gly Gly Glu Gln Ile Lys Val Gly Leu Arg Glu

245 250 255

Thr Lys Gly Being Met Asp Val Arg Tyr His Thr Pro Glu Glu Glu Ile

260 265 270

Wing Read Gly Pro Wing Cys Trp Read Trp Asp Tyr Read Le Arg Arg Be Arg

275 280 285

Thr Gln Gly Tyr Phe Leu Pro Leu Be Gly Gly Ile Asp Be Cys Ala290 295 300Thr Wing Ile Ile Val His Be Met Cys Arg Leu Val Val Glu Wing Ala305 310 315 320

Wing Lys Gly Asp Glu Gln Val Ile Thr Asp Wing Arg Arg Ile Thr Asn

325 330 335

Glu Pro Glu Asp Be Thr Tyr Ile Pro Glu Asp Pro Arg Glu Phe Ala

340 345 350

Gly Arg Ile Phe His Thr Cys Tyr Met Gly Thr Glu Asn Ser Ser Ser

355 360 365

Glu Thr Arg Glu Arg Wing Lys Asn Leu Wing Asp Wing Ile Gly Wing Tyr

370 375 380

His Val Asp Read Asn Met Asp Thr Val Wing Ser Val Wing Lys Gly Ile385 390 395 400

Phe Ser Phe Val Gly Lys Thr Pro Gln Phe Lys Wing His Gly Gly

405 410 415

Thr Asn Glu Wing Asn Leu Wing Leu Gln Asn Ile Gln Wing Arg Leu Arg

420 425 430

Met Val Val Ser Tyr Met Phe Wing Gln Read Leu Pro Trp Val Arg Gly

435 440 445

Lys Asn Gly Gly Leu Leu Val Leu Gly Ser Wing Asn Val Asp Glu Ser

450 455 460

Read Arg Gly Tyr Phe Thr Lys Tyr Asp Cys Ser Be Wing Asp Val Asn465 470 475 480

Pro Ile Gly Gly Ile Ser Lys Val Asp Read Lys Arg Phe Ile Wing Trp

485 490 495

Gln Wing Val Lys Phe Asp Leu Pro Ile Leu Tyr Asn Phe Leu His Wing

500 505 510

Val Pro Thr Wing Glu Leu Ile Pro Ile Gly Pro Asp Asn Ile Ile Gln

515 520 525

Ser Asp Glu Ile Glu. Met Gly Met Thr Tyr Asp Glu Read Ser Val Tyr

530 535 540

Gly Arg Leu Arg Lys Val Glu Lys Cys Gly Pro Phe Be Met Phe Gly545 550 555 560Lys Read Val -Gln Glu Trp Gly Be Phe Le Pro Be Lys Glu Ile Wing

565 570 575

Glu Lys Val Lys His Phe Phe Phe Met Tyr Ala Ile Asn Arg His Lys

580 585 590

Met Thr Thr Ile Thr Pro Be Val His Met Glu Be Tyr Be Pro Asp

595 600 605

Asp Asn Arg Phe Asp Read Arg Pro Phe Read Tyr Pro Be Gln Phe Thr

610 615 620

His Gln Phe Arg Lys Ile Asp Glu Read Wing Gly Lys Read Pro Asp Met625 630 635 640

Gln Lys Pro Wing Lys Val Asp Thr Asn Glu Val Asp645 650

<210> 26 <211> 677 <212> PRT

<213> Chaetomium globosum <400> 26

Met Gly His Leu Val Thr Val Wing Thr Cys Ser Leu Asn Gln Trp Val1 5 10 15

Read Asp Trp Glu Gly Asn Read Gly Arg Ile Ile Glu Be Ile His Gln

20 25 30

Lys Wing Gly Wing Arg Wing Arg Leu Arg Val Gly Pro Glu Leu Glu Ile

35 40 45

Cys Gly Tyr Being Asp Read Asp His Phe His Glu Read Asp Val Tyr Thr

50 55 60

His Ser Leu Glu Met Leu Wing Gln Leu Leu Gln Asp Lys Ser Thr His65 70 75 80

Gly Ile Leu Leu Asp Val Gly Ile Leu Leu His Arg Asn Leu Arg

85 90 95

Tyr Asn Cys Arg Val Ile Cys Leu Asp Gly Lys Ile Leu Leu Ile Arg

100 105 HO

Pro Lys Met Trp Leu Wing Asn Asp Gly Asn Tyr Arg Glu Met Arg His115 120 125

Phe Thr Pro Trp Met Arg Pro Arg Glu Thr Glu Leu Phe His Leu Pro

130 135 140

Lys Ile Leu Wing Glu Leu Gln Gly Glu Thr His Val Leu Phe Gly Asp145 150 155 160

Val Ile Wing Ser Thr Pro Glu Thr Phe Gly Wing Glu Wing Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Lys Wing Pro His Ile Asp Met Wing Leu Asp Gly

180 185 190

Val Glu Ile Ile Thr Asn Be Gly Be His Phe Thr Read Arg Lys

195 200 205

Leu Asp Thr Arg Leu Gln Leu Ile Met Glu Wing Thr Arg Lys Ser Gly

210 215 220

Gly Val Tyr Leu Tyr Wing Asn Gln Gln Gly Cys Asp Gly Glu Arg Leu225 230 235 240

Tyr Phe Asp Gly Cys Wing Met Ile Ile Val Asn

245 250 255

Gln Gly Be Gln Phe Be Read Asn Asp Val Glu Val Val Thr Wing Thr

260 265 270

Val Asp Leu Glu Glu Val Arg Be Tyr Arg Wing Ward Ile Be Arg Gly

275 280 285

Read Gln Wing Wing Wing Wing Be Thr Wing Lys Tyr Gln Arg Ile Gln Thr Pro

290 295 300

Phe Glu Read Be Ser Glu Asp Gly Asp Wing Asp Val Thr Val Wing Pro305 310 315 320

Thr Read Leu Ile Gln Pro Arg Tyr His Ser Val Glu Glu Glu Ile Wing

325 330 335

Leu Be Gly Gly Cys Tyr Leu Trp Asp Tyr Leu Arg Arg Be Gly Thr

340 345 350

Gly Wing Tyr Leu Val Pro Leu Gly Gly Ile Asp Ser Cys Ala Thr

355 360 365

Gly Wing Ile Val Tyr Ser Gave Le Cys Arg Ile Val Gly Gly Leu Gly370 375 380

Glu Gly Asn Lys Gln Val Leu Glu Glu Val Gln Arg Ile Pro Lys Tyr385 390 395 400

Gly Gly Glu Gly Val Phe Thr Thr Thr Pro Gln Glu Read Cys Lys Pro

405 410 415

Gly Phe Be Pro Ile Tyr Met Gly Met Lys Lys Gln Be Arg Glu

420 425 430

Thr Pro Gln Arg Wing Gln Asp Read Be Glu Wing Ile Gly Be Tyr His

435 440 445

Val Asn Leu Asp Ile Asp Asp Glu Val Gly Gln Met Leu Be Thr Wing

450 455 460

Arg Gln Arg Pro Gly Gly Gly Be Leu Read Val Leu Gly Be Wing Asn465 470 475 480

Val Gly Glu To Be Read Arg Gly Tyr To Read Thr Lys Tyr Asp Cys To Be Ser

485 490 495

Asp Wing Ile Asn Pro Ile Gly Ser Ile Asp Lys Asp Wing Read Lys Arg

500 505 510

Phe Ile Trp Wing Glu Wing Lys Asn Phe Ile Wing Pro Cys Leu His Glu

515 520 525

Phe Leu Thr Wing Val Pro Thr Wing Glu Leu Glu Pro Ile Thr Glu Asp

530 535 540

Tyr Val Gln Be Asp Glu Wing Asp Met Gly Met Thr Tyr Gln Glu Leu545 550 555 560

Thr Ile Phe Gly Arg Leu Arg Lys Leu Asn Lys Leu Gly Pro Phe Gly

565 570 575

Met Phe Gln Arg Read Val His Asp Trp Ser Ile Asp Arg Val Arg Lys

580 585 590

Pro Asp Asp Asp Pro Wing Tyr Tyr Thr Pro Thr Gln Val Wing Glu Lys

595 600 - 605

Val Lys Lys Phe Phe His Phe Tyr Ala Ile Asn Arg His Lys Met Thr

610 615 620

Thr Leu Thr Piro Wing. Read His Cys Asn Asp Tyx-Sejr Pro Asp Asp Asn625 630 635 640

Arg Phe Asp Leu Arg Pro Phe Leu Tyr Pro Pro Phe Trys Lys Ser Trp

645 650 655

Be Phe Lys Arg Ile Asp Met Glu Read Glu Lys Ile Glu Arg Lys Arg

660 665 670

Wing Ser Arg Lys Gln675

<210> 27 <211> 689 <212> PRT <213> Giberella zeae <400> 27 Met Asp Wing Read Ile Thr Read Wing Thr Cys Be Read Asn Gln Trp Val1 5 10 15 Read Asp Trp Glu Gly Asn Read Gly Arg Ile Arg Lys Ser Ile Ile Leu 20 25 30 Wing Lys Glu 35 Wing Gly Wing Wing Thr Leu 40 Arg Thr Gly Pro Glu 45 Leu Glu IleThr Gly Cyr Leu Asp His Phe Leu Glu Wing Asp Val Tyr Asp50 55 60 His Ser Leu Glu Ser Leu Leu Wing Ile Leu Thr Asp Thr Glu Leu His65 70 75 80Gly Ile Leu Ile Asp Val Gly Leu Pro Leu Met His Arg Gly Cys Arg 85 90 95 Tyr Asn Cys Arg 110 Pro Lys Ile Tyr Leu Wing Asn Asp Gly Asn Phe Arg Glu Asn Arg Phe 115 120 125 Phe Thr Pro Trp Asn Arg Pro Arg Tyr Val Glu Val Pro Ile Gly Asp145 150 155 160Val Ile Read Be Read Asn Asp Thr Thr Va l Wing Wing Glu Thr Cys Glu

165 170 175

Glu Leu Phe Thr Pro Gln Pro Wing His Ile Asn Met Wing Leu Asn Gly180 185 190

Val Glu Ile Phe Thr Asn Be Ser Gly Be His His Leu Arg Lys195 200 205

Leu Asn Glu Arg Leu Wing Leu Ile Be Glu Wing Thr Arg Lys Ser Gly

210 215 220

Gly Val Tyr Leu Tyr Wing Asn Gln Ser Gly Ser Asp Gly Asp Arg Leu225 230 235 240

Tyr Asp Gly As Ser Ser Leu Ile Met Val Asn Gly Asn Ile Val Wing

245 250 255

Gln Gly Be Gln Phe Be Read Asp Asp Val Glu Val Ile Thr Wing Thr260 265 270

Val Asp Leu Glu Glu Val Arg Wing Tyr Arg Phe Wing Pro Be Arg Asn275 280 285

Phe Gln Val Val Wing Gln Pro Val Val Wing Tyr Glu Arg Ile Glu Val Asp Phe

290 295 300

Ser Leu Gly Val Glu Asp Leu Asp Leu Leu Arg Wing Pro Thr Pro Pro305 310 315 320

Arg Pro Wing Arg Tyr His Val Glu Glu Glu Ile Wing Leu Val Leu

325 330 335

Val Arg Arg Be Lys Wing Be Gly Tyr Leu Val Pro Read Be Gly Gly340 345 350

Ile Asp Be Cys Wing Thr Thr Wing Ile Val Phe Be Met Cys Arg Leu355 360 365

Val Val Wing Ile Wing Lys Wing Gly Asn Glu Glu Val Ile Wing Asp Val

370 375 380

Lys Arg Ile Wing Val Tyr Ser Asp Lys Leu Pro Glu Thr Wing Glu Glu385 390 395 400

Phe Cys Asn Gln Ile Phe His Val Val Tyr Met Gly Met Glu Lys Gln405 410 415Be Being Lys Glu Thr Arg Gln Arg Wing Lys Asp

420 425 430

Gly Ser Tyr His Thr Asp Met Asn Ile Asp Asp Thr Phe Asn Wing Thr

435 440 445

Lys Asn Read Leu Thr Gln Wing Thr Gly Phe Glu Pro Lys Phe Lys Val

450 455 460

His Gly Gly Ser Wing Thr Glu Asn Leu Wing Leu Gln Asn Ile Gln Ala465 470 475 480

Arg Ser Arg Met Val Val Wing Tyr Tyr Tyr Wing Gln Met Leu Pro Thr

485 490 495

Val Arg Gln Arg Pro Gly Gly Gly Ser Leu Valu Gly Gly Ser Leu

500 505 510

Asn Val Asp Glu Cys Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Ser

515 520 525

Ser Asa Val Wing Asn Pro Ile Gly Ser Val Ser Lys Thr Asp Leu Lys

530 535 540

Arg Phe Ile Wing Trp Be Wing Lys Be Phe Asn Met Pro Ile Leu Glu545 550 555 560

Glu Phe Ile His Wing Thr Pro Asp Met Gly Met Thr Tyr Asp Glu Leu

565 570 575

Be Arg Phe Gly Arg Read Lys Arg Lys Glu Be Lys Read Gly Pro Tyr Gly

580 585 590

Met Phe Leu Arg Leu Leu Glu Glu Trp Gly Gly Gly Lys Met Thr

595 600 605

Pro Arg Asp Val Wing Thr Lys Val Lys Arg Phe His Gly Phe His Tyr

610 615 620

Ile Asn Arg His Lys Gln Val Val Wing Thr Pro Val Val Val Wing Glu.625 630 635 640

Asn Tyr Ser Asp Asp Asp His Arg Phe Asp Leu Arg Leu Pro Val Tyr

645 650 655

Pro Be Pro Trp Asn Be Trp Be Phe Glu Ile Asp Lys Arg Val660 665 670Glu Wing Ile Glu Arg Wing Met Glu Lys Lys Lys Thr Glu Ile Ser675 680 685

Lys <210> 28 <211> 674 <212> PRT

<213> Aspergillus fumigatus <400> 28

Met Leu Wing Arg Ile Ile Asp His Pro Asp Cys Gln Asp Ile Val Val1 5 10 15

Asp Val Gly Met Pro Val Arg His Arg Asn Val Arg Tyr Asn Cys Arg

20 25 30

Val Ile Phe Tyr Asn Arg Lys Ile Ile Read Ile Arg Pro Lys Met Trp

35 40 45

Leu Wing Asn Gly Ile Leu Phe Ser Leu Wing Val Ile Phe Leu Pro Thr

50 55 60

Ser Val Gln Leu Phe Thr Asn His Val Wing Arg Wing Ser Asp Gly Asn65 70 75 80

Tyr Arg Glu Read His Arg Phe Ser Pro Trp Gln Arg Pro Arg Glu Ile

85 90 95

Glu Asp Tyr Tyr Leu Glu Gln Ile Val Gly Lys Ile Thr Gly Gln Tyr

100 105 110

Lys Val Pro Phe Gly Asp Wing Val Ile Be Thr Arg Asp Thr Cys Ile

115 120 125

Gly Leu Glu Thr Cys Glu Glu Leu Phe Thr Asn Gly Pro His Ile

130 135 140

Pro Tyr Gly Leu Wing Gly Val Glu Ile Ile Ser Asn Ser Ser Gly Ser145 150 155 160

His His Glu Read Lys Lys Read Asp Thr Arg Val Asn Read Ile Thr Gln

165 170 175

Wing Thr Lys Leu Be Gly <51y Ile Tyr Leu Tyr Wing Asn Gln Gln Gly180 185 190Cys Asp Gly Asp Arg Leu Tyr Tyr Asp Gly Cys Ala Met Ile Val Ile

195 200 205

Asn Gly Asn Ile Val Wing Gln Gly Be Gln Phe Be Read Lys Asp Val

210 215 220

Glu Val Ile Thr Wing Val Thr Asp Ile Glu Glu Val Arg His Pro Val225 230 235 240

Arg Thr Tyr Arg Wing Be Ser Be Arg Asn Met Gln Wing Thr Arg Gln

245 250 255

Pro Pro Phe Val Arg Leu Asp Leu Asp Val Arg Leu Ser As Arg Leu Asp

260 265 270

Asp Asp Glu Pro Wing Gly Leu Val Pro Be Glu Pro Ile Be Wing Lys

275 280 285

Tyr His Wing Pro Glu Glu Glu Ile Ser Leu Gly Pro Wing Cys Trp Leu

290 295 300

Trp Asp Tyr Leu Arg Arg Be Gly Wing Gly Phe Phe Leu Pro Leu305 310 315 320

Be Gly Gly Ile Asp Be Cys Wing Thr Wing Ile Ile Val His Be Met

325 330 335

Cys Arg Glu Val Val Lys Wing Val Ser Glu Gly Asn Gln Gln Val Ile

340 345 350

Lys Asp Val Arg Arg Leu Cys Wing Glu Pro Glu Gly Be Thr Trp Leu

355 360 365

Pro Arg Thr Be Gln Glu Val Cys Asn Arg Ile Phe His Thr Be Phe

370 375 380

Met Gly Thr Gln Asn Be Ser Lys Glu Thr Arg Glu Arg Wing Lys Wing .385 390 395 400

Read Be Thr Glu Ile Gly Be Tyr His Ile Asp Phe Asn Phe Asp Thr

405 410 415

Val Val Thr Wing Ile Thr Asn Leu Phe Thr Val Ile Thr Asn Phe Gln

420 425 430

Pro Arg Phe Lys Val His Gly Gly Thr Gly Wing Glu Asn Wing Wing Leu435 440 445Gln Asn Val Gln Wing Arg Leu Arg Met Val Leu Ser Tyr Leu Phe Wing

450 455 460

Be Read Leu Pro Thr Val Arg Gln Arg Pro Gly Gly Gly Gly Leu Leu465 470 475 480

Val Leu Wing Be Ser Asn Val Asp Asp Leu Lys Lys Phe Ile Wing Trp

485 490 495

Wing Arg Asp Be Phe Asp Leu Pro Ile Read His Asp Phe Leu Thr Wing

500 505 510

Thr Pro Thr Glu Wing Leu Glu Pro Ile Thr Wing Thr Tyr Val Gln Ser515 520 525

Asp Glu Wing Asp Met Val Gly Val Thr Tyr Glu Wing Read Gly Thr Phe Gly

530 535 540

Tyr Leu Arg Lys Val Wing Lys Leu Gly Pro Trp Ser Met Tyr Glu Lys545 550 555 560

Leu Leu His Val Trp Gly Asn Glu Tyr Ser Pro Arg Glu Ile Tyr Glu

565 570 575

Lys Thr Arg His Phe Phe Tyr His Tyr Ala Ile Asn Arg His Lys Met

580 585 590

Thr Val Leu Thr Pro Be Tyr His Wing Glu Gln Tyr Be Pro Glu Asp595 600 605

Asn Arg His Asp Leu Arg Gln Phe Leu Cys Gln Leu Leu Be Ser Thr

610 615 620

Asp Gly Leu Leu Wing Arg Val Asn Wing Asp Leu Cys Leu Leu Asp Pro625 630 635 640

Pro Phe Thr Trp Wing Tyr Lys Lys Met Glu Glu Ser Val Lys Tyr Trp

645 650 655

Glu Be Lys Gly Trp Thr Wing Gly Lys Wing Gln Lys Lys Be Val Lys660 665 670

Asp wing

<210> 29 <211> 1315 <212> PRT <213> Magnaporthe grisea <400> 29

Met Arg Phe Val Thr Val Ward Wing Ward Thr Leu Pro Ser Val Pro Leu1 5 10 15

Asp Phe Glu Gly Asn Arg Asp Arg Ile Leu Glu Ser Ile Lys Leu Wing

20 25 30

Lys Glu Lys Gly Wing Thr Read Le Arg Thr Gly Pro Glu Leu Glu Ile Pro

35 40 45

Gly Tyr Gly Cys Leu Asp His His Leu Glu Gly Asp Thr Glu Leu His

50 55 60

Ser Trp Glu Val Leu Wing Glu Ile Ile Ser Asp Pro Val Cys Lys Asp65 70 75 80

Met Leu Val Asp Leu Gly Leu Gly Val Lys Thr Arg Asn Val Gln Tyr

85 90 95

Asn Cys Arg Val Leu Cys Thr Tyr Lys Lys Ile Tyr Wing Ile Arg Wing

100 105 110

Lys Gln Wing Read Wing Gly Asp Wing Gly Read Tyr Arg Glu Pro Arg His Phe

115 120 125

Thr Wing Trp Val Lys Glu Arg Gln Val Glu Thr His Lys Read His Lys

130 135 140

Val Val Arg Asp Val Val Gly Gln Val Val Thr Ile Gly Val Ashe Phe145 150 155 160

Ile Leu Glu Thr Pro Asp Thr Be Val Thr Cys Glu Thr Cys Glu Glu

165 170 175

Read Phe Val Pro Arg Asn Pro Ile Phe Ser Gly Read Asn Gly Ala Wing

180 185 190

Glu Ile Ile Leu Asn Be Sera Wing Be His Wing Glu Leu Arg Lys Leu

195 200 205

Gly Thr Arg Read Asn Read Ile Be Asn Be Thr Asr Be Asn Gly Gly

210 215 220

Leu Tyr Val Tyr Wing Asn Wing Ser Gly Ile Asp Gly Glu Wing Arg Met225 230 235 240Leu Phe Asp Gly Being Met Ile Ile Gln Asn Gly Glu Val Leu Wing

245 250 255

Gln Ser Be Gln Phe Be Read Leu Pro Val Glu Val Thr Val Wing Thr260 265 270

Val Asp Leu Glu Arg Val Arg Be Tyr Arg Thr Be Wing Be Arg Asn275 280 285

Val Gln Wing Arg Wing Gln Pro Glu Tyr Pro Arg Ile Asp Cys Asp Ile

290 295 300

Glu Leu Arg Wing Pro Be Glu Glu Ile Phe Arg Be Asn Lys Val Ile305 310 315 320

Wing Met Glu Ile Pro Ile Arg Ile Read Asp Pro Met Glu Glu Ile His

325 330 335

Met Wing Thr Be Val Tyr Leu Trp Gln Tyr Leu Val Arg Be Gly340 345 350

Wing Gly Phe Phe Leu Wing Read Le Ser Gly Gly Leu Asp Ser Ser Val355 360 365

Wing Leu Phe Val Tyr Gly Met Wing Lys Leu Val Leu Leu Ser Ile Lys

370 375 380

Asn Gly Glu Glu Asn Thr Read Asn Asp Asp Read Arg Lys Val Thr Gly Ile385 390 395 400

Asn Asp Tyr Val Glu Pro Glu Glu Pro Ile Val Gly Lys Leu Leu

405 410 415

His Thr Cys Phe Met Gly Thr Val Asn Be Ser Asp Glu Thr Arg Ser420 425 430

Arg Wing Lys Arg Wing Read Glu Wing Arg Read Gly Wing Tyr His Thr Asp Ile435 440 445

Asn Ile Asp Asn Wing Val Gln Wing His Glu Ser Ile Ile Glu Ser Wing

450 455 460

Gly Gly Phe Lys Pro Lys Tyr Wing Val Glu Gly Gly Thr Asn Ser465 470 475 480

Glu Asn Leu Wing Lys Gln Asn Ile Gln Arg Wing Arg Asn Arg Leu Val Val485 490 495Ser Tyr Glu Leu Wing Gln Leu Be Thr Gln Wing Arg Gly Leu Pro Arg

500 505 510

Gly Wing Ala Ser Leu Leu Val Leu Gly Ser Gly Asn Val Asp Glu Asn

515 520 525

Read Arg Gly Tyr Tyr Thr Lys Tyr Asp Ala Ser Be Ala Asp Leu Ala

530 535 540

Pro Read Gly Ser Ile Ser Lys Asn Asp Asp Wing Lys Asp Phe Gln Arg Trp545 550 555 560

Wing Arg Asp Asn Trp Asp Read Ile Met Be Glu Phe Ile Asp Wing

565 570 575

Ile Pro Be Glu Wing Read Leu Pro Read Be Gla Wing Gly Val Gln Wing Asp

580 585 590

Glu Val Glu Met Gly Read Thr Tyr Be Glu Read Asp Phe Gly Ile

595 600 605

Leu Arg Lys Val Asp Lys Leu Gly Pro Trp Ser Wing Tyr Leu Arg Leu

610 615 620

Leu Be Gln Trp Lys Glu Arg Pro Gly Phe Gly Pro Arg Glu Ile Ala625 630 635 640

Glu Lys Val Phe Leu Phe Phe Arg Phe Tyr Ala Ile Asn Arg His Lys

645 650 655

Wing Thr Ile Ile Thr Pro Be Val His Leu Be Wing Tyr Asn Pro Asp

660 665 670

Asp Asn Arg His Asp Asp Asn Asn Arg Glu Lys Lys Ser Ile Wing Ser

675 680 685

Ile Trp Gln Be Gln Wing Phe Be Wing Thr Gly Asp Leu Pro Thr Leu

690 695 700

Leu Leu Wing Gly Wing Lys Leu Pro Lys Ile Asn Pro Be Leu Arg Arg705 710 715 720

Lys Cys Asp Be Arg Read Asp Be Asn Gly Lys Cys Arg Be Pro

725 730 735

Gly Val Thr Pro Asn Phe Gln Pro Gln Pro Wing His Leu Gln Val Leu740 745 750Thr Pro To Be Thr Be Asn Arg Leu Glu Ile Asp Wing Thr Be Arg Wing

755 760 765

His Phe Ile Gly Trp Leu Cys Gly Ser Leu Leu Thr Wing Ala Val Ser

770 775 780

Wing Thr Asn Phe Wing Asn Cys Tyr His Leu Wing Phe Glu Glu Phe Arg785 790 795 800

Asn Gly Thr His Pro Met Arg Asp Being Phe Phe Trp Lys Glu Lys Asp

805 810 815

Pro Leu Ile Gly Leu Lys Asn Gln Ser Wing Arg Pro Glu Ile Val Leu

820 825 830

Thr Arg Wing Gly Cys Glu His Phe Cys Ser Ile Tyr Pro Gln Tyr Asn

835 840 845

Asn Val Ile Asp Phe Wing Gln Ile Read Thr Thr Trp Val Leu Pro Wing

850 855 860

Ile Wing Read Met Be Gln Read Pro Tyr Glu Be Read Ser Val Val Trp865 870 875 880

Arg Lys Asn Leu Wing Wing Phe Gly Wing Trn Val Gly Wing Pro Wing Wing

885 890 895

Ala Met Thr Thr Thr Phe Asp Ile Val Leu Ile His Glu Cys Ser

900 905 910

Val Lys Phe Gly Leu Phe Arg Leu Pro Glu Thr Arg Glu Glu Ile Arg

915 920 925

Asp Wing Read Phe Ile Read Cys Val Asn Gln Tyr Glu Tyr Pro Arg

930 935 940

Arg Glu Gln Val Gly Arg Asp Leu Arg Arg Asp Be Wing Leu Leu Lys945 950 955 960

Gly Ile Read Tyr Pro Tyr Phe Lys Asp Gly Ile Be Glu Arg Gln Arg

965 970 975

Arg Thr Read Ala Asn Phe Asn Gln His Leu Ala Phe Gln Leu Arg Leu

980 985 990

Gln Arg Arg Lys Gly Val Tyr Pro Ile Phe Ile Be Ile Gly Trp Phe995 1000 1005Gly Met Wing Phe Wing Phe Be Ile Val Ile Be Phe Wing Wing Leu

1010 1015 1020

Gly Asp Asn Thr Thr Wing His Being Leu Wing Leu Gly Leu Leu Leu

1025 1030 1035

Ser Trp Val Pro Val Met Leu Phe Ala Thr Val Ile Asp Arg Asn

1040 1045 1050

Pro Thr Be Wing Thr Arg Cys Be Glu Leu Ile Gln Arg Trp Leu

1055 1060 1065

Phe Asn Ile Asp Arg Leu Leu Pro Ala Thr Asp Leu Be Ser Leu

1070 1075 1080

Pro Lys Phe Trp Gln Wing His His Gly Gly Gln Lys His Wing

1085 1090 1095

Asp Thr Thr Glu Glu Phe Asp Ile Gly Glu Tyr Met Gly Gln Gly

1100 1105 1110

Arg Thr Read Le Arg Tyr Cys Gly Val Wing Asp Thr Thr Le Le Asp Met

1115 1120 1125

Ile Lys Asn Pro Be Glu Wing Thr Read Asp Val Pro Asp Read Le

1130 1135 1140

Val Gly Lys Lys His Wing Lys Phe Be Asp Arg Be Asp Glu Wing

1145 1150 1155

Thr Val Gly Met Gly Gly Gly Val Vala His Gln Pro Gly His Cys

1160 1165 1170

Asp His Gln Leu Arg His Gly Leu His Gly Val Val Gln His Wing

1175 1180 1185

Asn Wing Gly Ser Gly Leu Ser Leu Wing Ala Leu Pro Leu Leu Val

1190 1195 1200

Wing Pro Gly Ser Wing Wing Val Val Wing Asp Pro Gly Winged Ser Gly

1205 1210 1215

Gln Wing His Gln Met Gln Be Phe Gly Gly Leu Asn Be Cys Leu

1220 1225 1230

Cys Lys Thr Be Val Phe Gly Leu Pro Pro Phe Gly Arg Tyr Met1235 1240 1245Asp Phe Glu Asn Wing Glu Phe Tyr Lys Glu Tyr Tyr Tyr Val Glu

1250 1255 1260

Wing Phe Trp Gly Be Wing Thr Gly Ile Wing Gly Gly Wing Thr Met Wing

1265 1270 1275

Ile Ser Val Ser Trp Read Wing Trp Lys Trp His Lys Ser Ser Wing

1280 1285 1290

Leu Trp Arg Val Val Glu Asp Thr Thr Val Val Val Glu Asp

1295 1300 1305

Val Ser Leu Asp Trp Leu Thr1310 1315

<210> 30 <211> 401 <212> PRT

<213> Saccharomyces cereviseae <400> 30

Met Asp Pro Thr Arg Wing Pro Asp Phe Lys Pro Wing Pro Asp Glu1 5 10 15

Glu Leu Ile Pro Pro Asp Pro Glu Ser Lys Ile Pro Lys Ser Ile

20 25 30

Pro Ile Ile Pro Tyr Val Leu Wing Asp Wing Asn Ser Ser Ile Asp Wing

35 40 45

Pro Phe Asn Ile Lys Arg Lys Lys Lys His

50 55 60

His His His Arg Lys Glu Gly Asn Asp Lys Lys His Gln His Ile65 70 75 80

Pro Leu Asn Gln Asp Asp Phe Gln Pro Leu Ser Wing Glu Val Ser Ser

85 90 95

Glu Asp Asp Asp Wing Asp Phe Arg Be Lys Glu Arg Tyr Gly Be Asp

100 105 110

Be Thr Thr Glu Be Glu Thr Arg Gly Val Gln Lys Tyr Gln Ile Wing

115 120 125

Asp Leu Glu Glu Val Pro His Gly Ile Val Arg Gln Wing Arg Thr Leu130 135 140

Glu Asp Tyr Glu Phe Pro To Be His Arg Read To Be Lys Lys Read To Read Asp145 150 155 160

Pro Asn Lys Leu Pro Leu Val Ile Val Wing Cys Gly Ser Phe Ser Pro

165 170 175

Ile Thr Tyr Leu His Leu Arg Met Phe Glu Met Wing Leu Asp Wing Ile

180 185 190

Being Glu Gln Thr Arg Phe Glu Val Ile Gly Gly Tyr Tyr Being Pro Val

195 200 205

Be Asp Asn Tyr Gln Lys Gln Gly Read Ala Pro Be Tyr His Arg Val

210 215 220

Arg Met Cys Glu Leu Wing Cys Glu Arg Thr Be Ser Trp Leu Met Val225 230 235 240

Asp Wing Trp Glu Be Read Gln Pro Be Tyr Thr Arg Thr Wing Lys Val

245 250 255

Read Asp His Phe Asn His Glu Ile Asn Ile Lys Arg Gly Gly Val Wing

260 265 270

Thr Val Thr Gly Glu Ile Gly Val Lys Ile Met Leu Read Leu Gly Wing

275 280 285

Gly Asp Leu Ile Glu Be Met Gly Glu Pro Asn Val Trp Wing Asp Wing

290 295 300

Asp Read His His Ile Read Gly Asn Tyr Gly Cys Read Ile Val Glu Arg305 310 315 320

Thr Gly Be Asp Val Arg Be Phe Leu Read His Be Asp Ile Met Tyr

325 330 335

Glu His Arg Arg Asn Ile Leu Ile Ile Lys Gln Leu Ile Tyr Asn Asp

340 345 350

Ile Be Ser Thr Lys Val Arg Leu Phe Ile Arg Arg Wing Met Ser Val

355 360. 365

Gln Tyr Leu Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Glu His Arg

37-0 375 380

Leu Tyr Val Asp Gln Thr Glu Pro Val Lys Gln Val Leu Gly Asn Lys385 390 395 400

Glu

<210> 31 <211> 411 <212> PRT

<213> Candida glabrata <400> 31

Met Asp Pro Thr Asp Pro Asp Phe Lys Pro Arg Asp Pro Asp Ala1 5 10 15

Lys Leu Leu Pro Pro Asp Pro Thr Be Lys Ile Pro Lys Be Gly

20 25 30

Pro Ile Thr Pro Tyr Val Leu Wing Asp Tyr Asn Wing Ser Ile Asp Wing

35 40 45

Pro Leu Read Gln Met Lys Asys Thr Thr His Leu Pro His Be Gln Gly

50 55 60

Asn His Gln Arg Ile Pro Read Asn Lys Ser Glu Phe Gln Pro Leu Ser65 70 75 80

Thr Thr Gly Ser Be Glu Glu Asp Phe Glu Read Asp Asp Glu Asn Met

85 90 95

Asp Be Asp Be Gly Be Lys Arg Glu Be Read Gly Gln Read Be Gly

100 105 110

His Gly Ile Gly Ile Gly Val His Lys Asn Gln Ile Wing Asp Leu Glu

115 120 125

Glu Val Pro His Gly Ile Val Arg Gln Wing Arg Phe Leu Glu Asp Tyr

130 135 140

Glu Phe Pro Thr His Arg Read Wing Asn Arg Read Le Gln Asp Pro Asn Lys145 150 155 160

Met Pro Read Val Ile Val Cys Wing Gly Ser Phe Ser Pro Ile Thr Tyr

165 170 175

Leu His Leu Arg Met Phe Glu Met Wing Leu Asp Wing Ile Ser Glu Met

180 185 190

Thr Arg Phe Glu Val Val Gly Gly Tyr Tyr Ser Pro Val Ser Asp Asn195 200 205

Tyr Lys Lys Gln Gly Read Wing Pro Be Tyr His Arg Val Arg Met Cys

210 215 220

Glu Leu Wing Cys Glu Arg Thr Be Ser Trp Leu Met Val Asp Wing Trp225 230 235 240

Glu Be Leu Gln Pro Be Tyr Thr Arg Thr Wing Lys Val Leu Asp His

245 250 255

Phe Asn His Glu Ile Asn Val Lys Gly Gly Ile Thr Val His Glu

260 265 270

Lys Lys Arg Asn Asp Wing Asn Ser Gly Tyr His Met Glu Glu His Lys

275 280 285

Arg Gly Val Lys Ile Met Leu Leu Wing Gly Gly Asp Leu Ile Glu Ser

290 295 300

Met Gly Glu Pro Gly Val Trp Wing Asp Glu Asp Read His His Ile Leu305 310 315 320

Gly Asn Tyr Gly Cys Leu Ile Val Glu Arg Thr

325 330 335

Be Phe Read Leu Be His Asp Ile Met Tyr Glu His Arg Arg Asn Val

340 345 350

Leu Val Ile Lys Gln Leu Ile Tyr Asn Asp Ile Be Ser Thr Lys Val

355 360 365

Arg Leu Phe Ile Arg Arg Asn Met Ser Val Gln Tyr Leu Leu Pro Asn

370 375 380

Ser Val Ile Arg Tyr Ile Gln Glu His Lys Read Tyr Ile Asn Gln Ser385 390 395 400

Glu Pro Val Lys Gln Val Leu Ser Gly Lys Glu405 410

<210> 32 <211> 400 <212> PRT

<213> Eremothecium gossypii <400> 32Met Asp Pro Thr Arg Wing Pro Asp Phe Lys Pro Read Gln Gly Asp Asp15 10 15

Lys Pro Glu Pro Pro Read Wing Pro Asp Be Glu Ile Pro Glu Asn Gly20 25 30

Pro Ile Met Pro Phe Val Leu Asp Wing Tyr Asn Thr Ser Ile Asp Wing 35 40 45

Pro Ile His Pro Lys Be Tyr Lys Be Met Lys Arg Asn Be Ser

50 55 60

Gln Pro Being Asn Cys Being Lys Gly Arg Gly Gly Being Gly Being Ile Pro65 70 75 80

Leu Lys Arg Be Gly Leu Glu Pro Phe Ser Asn Asp Val Be Ser Glu

85 90 95

Be Glu Cys Gly Glu Glu Asp Glu Gln Gln Gly Glu Be His Wing Ala100 105 110

Asp Wing Asn Be Ser Lys Leu Arg Be Met Val Gln Asn Ile Wing Asp115 120 125

Leu Glu Glu Val Pro Cys Gly Ile Ile Arg Gln Wing Arg Ile Leu Glu

130 135 140

Asp Tyr Glu Phe Pro Thr His Arg Read Lys Thr Thr Read His Asn Ala145 150 155 160

Asn Lys Leu Pro Leu Val Ile Val Wing Cys Gly Ser Phe Leu Pro Ile

165 170 175

Thr His Leu His Leu Arg Met Phe Glu Met Wing Met Asp Wing Ile Val180 185 190

Glu Gln Thr Arg Phe Glu Val Gly Gly Tyr Tyr Tyr Ser Pro Val Ser195 200 205

Asp Asn Tyr Asn Lys Pro Gly Leu Wing Be Wing Thr His Arg Val Arg

210 215 220

Met Cys Glu Leu Wing Cys Glu Arg Thr Be Ser Trp Leu Met Val Asp225 230 235 240

Trp Glu Wing Being Read Gln Pro Gln Tyr Thr Arg Thr Lys Val Wing Leu245 250 255Asp His Phe Asn Asp Glu Ile Asn Val Lys Arg Gly Gly Ile Lys Thr

260 265 270

Be Thr Gly Asp Arg Ile Gly Val Lys Ile Met Read Leu Wing Gly Gly

275 280 285

Asp Leu Ile Glu Be Met Gly Glu Pro Asn Val Trp Wing Asp Wing Asp

290 295 300

Read His His Ile Read Gly Asn Tyr Gly Cys Read Ile Val Glu Arg Thr305 310 315 320

Gly Be Asp Val Arg Be Phe Leu Read His Be Asp Ile Met Tyr Glu

325 330 335

His Arg Arg Asn Ile Read Val Ile Lys Gln Met Ile Tyr Asn Asp Ile

340 345 350

Being Being Thr Lys Val Arg Read Phe Ile Arg Arg Gly Met Being Val Gln

355 360 365

Tyr Leu Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Glu Tyr Gly Leu

370 375 380

Tyr Val Asn Glu His Glu Pro Val Gln Gln Val Ile Ser Asn Lys Asp385 390 395 400

<210> 33 <211> 449 <212> PRT

<213> Kluyveromyces lactis <400> 33

Met Asp Pro Thr Arg Asp Pro Asn Phe Lys Ser Pro Gln Glu Read Asp1 5 10 15

Be Glu Wing Leu Gln Be Thr Asp Ile Pro Lys Ile Gly Pro Ile

20 25 30

Ile Pro Tyr Val Leu Asp Wing Tyr Asn Thr Wing Ile Asp Lys Pro Ile

35 40 45

Arg Pro Arg Lys Tyr Lys Lys Asn Wing Ile Lys Arg Lys Read Wing Al

50 55 60

Be Met Thr Be Thr Be Asp Asp Ser Asn Glu Gln Gln His Leu Ser65 70 75 80

Wing Asn Ser Asn Asn Gly Phe Ile Pro Read Lys Gln Arg Glu Read Le Gln

85 90 95

Pro Val Be Wing Glu Thr Be Gly Asp Be Glu Be Glu Be Glu Be

100 105 110

Pro Wing Glu Pro Wing Asn Val Asp Read Asp Ile Asp Pro Asn Pro Glu

115 120 125

Be Asp Glu Val Met Wing Ser Pro Wing Val Wing His Wing Ser Gly Ser

130 135 140

Val Ser Gly Pro Ser Gly Met Wing Wing Wing Wing Wing Wing Wing Wing Ser145 150 155 160

Read Asp Gln Glu Ile Thr Be Arg Ile His Lys Phe Gln Ile Wing Asp

165 170 175

Leu Glu Glu Val Pro His Gly Ile Val Arg Gln Wing Asn Asn Trp Lys

180 185 190

Asp Tyr Gln Phe Pro Wing His Arg Read Lys Gln Arg Read Arg Asn Ser

195 200 205

Asn Lys Leu Pro Leu Val Ile Val Wing Cys Gly Ser Phe Ser Pro Ile

210 215 220

Thr Tyr Leu His Leu Arg Met Phe Glu Met Wing Read Asp Wing Ile Ser225 230 235 240

Glu Gln Thr Arg Phe Glu Val Ile Gly Gly Tyr Tyr Ser Pro Val Ser

245 250 255

Asp Asn Tyr Lys Lys Pro Gly Leu Pro Wing His His Arg Val Arg

260 265 270

Met Cys Glu Leu Gly Cys Glu Arg Thr Be Ser Trp Leu Met Val Asp

275 280 285

Wing Trp Glu Ser Leu Gln Pro Thr Tyr Thr Arg Thr Wing Met Val Leu

290 295 300

Asp His Phe Asn Glu Glu Ile Asn Val Lys Arg Lys Gly Val Ile Lys305

Asn Asp Wing Gly Glu Arg Met Gly Val Lys Ile Met Leu Read Wing Gly325 330 335

Gly Asp Leu Ile Glu Be Met Gly Glu Pro Asn Val Trp Wing Asp Tyr

340 345 350

Asp Read His His Ile Leu Gly Asn Tyr Gly Cys Leu Ile Val Glu Arg

355 360 365

Thr Gly Be Asp Val Arg Be Phe Leu Read His Be Asp Ile Met Tyr

370 375 380

Glu His Arg Arg Asn Ile Read Val Ile Lys Gln Read Ile Tyr Asn Asp385 390 395 400

Ile Ser Be Thr Lys Val Arg Leu Phe Ile Arg Be Arg Arg Met Be Val

405 410 415

Gln Tyr Leu Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Glu His Gly

420 425 430

Read Tyr Val Asn Gln Thr Glu Pro Val Lys Gln Val Ile Gly Asn Lys435 440 445

Asp

<210> 34 <211> 400 <212> PRT

<213> Debyaromyces hansenii <400> 34

Met Asp Pro Thr Arg Wing Pro Asp Phe Lys Pro Read Gln Gly Asp Asp1 5 10 15

Lys Pro Glu Pro Pro Read Wing Pro Asp Be Glu Ile Pro Glu Asn Gly

20 25 30

Pro Ile Met Pro Phe Val Leu Asp Wing Tyr Asn Thr Be Ile Asp Wing

35 40 45

Pro Ile His Pro Lys Be Tyr Lys Be Met Lys Arg Asn Be Ser

50 55 60

Gln Pro Being Asn Cys Being Lys Gly Arg Gly Gly Being Gly Being Ile Pro65 70 75 80

Leu Lys Arg Be Gly Leu Glu Pro Phe Ser Asn Asp Val Ser Ser Glu85 90 95

Be Glu Cys Gly Glu Glu Asp Glu Gln Gln Gly Glu Be His Wing Wing

100 105 110

Asp Wing Asn Be Ser Lys Leu Arg Be Met Val Gln Asn Ile Asp Wing

115 120 125

Leu Glu Glu Val Pro Cys Gly Ile Ile Arg Gln Wing Arg Ile Leu Glu

130 135 140

Asp Tyr Glu Phe Pro Thr His Arg Read Lys Thr Thr Read His Asn Ala145 150 155 160

Asn Lys Leu Pro Leu Val Ile Val Wing Cys Gly Ser Phe Leu Pro Ile

165 170 175

Thr His Leu His Leu Arg Met Phe Glu Met Wing Met Asp Wing Ile Val

180 185 190

Glu Gln Thr Arg Phe Glu Val Val Gly Gly Tyr Tyr Ser Pro Val Ser

195 200 205

Asp Asn Tyr Asn Lys Pro Gly Leu Wing Be Wing Thr His Arg Val Arg

210 215 220

Met Cys Glu Leu Wing Cys Glu Arg Thr Be Ser Trp Leu Met Val Asp225 230 235 240

Wing Trp Glu Being Read Gln Pro Gln Tyr Thr Arg Thr Wing Lys Val Leu

245 250 255

Asp His Phe Asn Asp Glu Ile Asn Val Lys Arg Gly Gly Ile Lys Thr

260 265 270

Be Thr Gly Asp Arg Ile Gly Val Lys Ile Met Read Leu Wing Gly Gly

275 280 285

Asp Leu Ile Glu Be Met Gly Glu Pro Asn Val Trp Wing Asp Wing Asp

290 295 300

Read His His Ile Read Gly Asn Tyr Gly Cys Read Ile Val Glu Arg Thr305 310 315 320

Gly Be Asp Val Arg Be Phe Leu Read His Be Asp Ile Met Tyr Glu

325 330 335

His Arg Arg Asn Ile Leu Val Ile Lys Gln Met Ile Tyr Asn Asp Ile340 345 350

Being Being Thr Lys Val Arg Read Phe Ile Arg Arg Gly Met Being Val Gln

355 360 365

Tyr Leu Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Glu Tyr Gly Leu

370 375 380

Tyr Val Asn Glu His Glu Pro Val Gln Gln Val Ile Ser Asn Lys Asp385 390 395 400

<210> 35 <211> 401 <212> PRT

<213> Candida albicans <400> 35

Met Asp Pro Thr Asn Asp Pro Asn Phe Thr Pro Pro Be Read Asn Lys1 5 10 15

Asn Ile Glu Pro Thr Wing Pro Be Asp Lys Ile Pro Be Ile Met Pro

20 25 30

Ile Gln Pro Phe Val Leu Glu Glu Leu Gly Glu Gly Val Asp Gly Pro

35 40 45

Val Pro His Pro Wing Be Ser Be Arg Pro Thr Thr Thr Wing Arg

50 55 60

Be Tyr Asp Wing Be His Wing Be Asp Thr Glu Be Lys Tyr His Ser65 70 75 80

Lys Ile Pro Arg Lys His Thr Glu Read Ile Be Ser Ser Ser Ser Asp

85 90 95

Glu Glu Asn Glu Pro Read Ser Lys Pro Pro Glu Ile Ile Pro Pro Lys

100 105 110

Lys Glu Ile Leu Pro Pro Be Ile Lys Val Arg Be Ser Gln Ile Wing

115 120 125

Asp Leu Glu Glu Val Pro His Gly Ile Gln Arg Gln Wing Leu Lys Leu

130 135 140

Glu Asp Tyr His Phe Pro Thr His Arg Leu Wing Thr Thr Leu Thr Asp145 150 155 160Asp Ser Lys His Pro Leu Val Ile Val Wing Cys Gly Ser Phe Ser Pro

165 170 175

Ile Thr Tyr Leu His Leu Arg Met Phe Glu Met Wing Leu Asp Wing Ile

180 185 190

Thr Glu Gln Thr Arg Phe Glu Val Ile Gly Gly Tyr Tyr Ser Pro Val

195 200 205

Be Ser Asn Tyr Lys Lys Gln Gly Read Ward Pro Ward His His Arg Val

210 215 220

Arg Met Cys Glu Leu Wing Cys Glu Arg Thr Be Ser Trp Leu Met Val225 230 235 240

Asp Wing Trp Glu Being Read Gln Pro Lys Tyr Thr Arg Thr Wing Leu Val

245 250 255

Read Asp His Phe Asn Glu Glu Ile Asn Ile Lys Gln Gly Gly Ile Met

260 265 270

Thr Arg Be Gly Glu Lys Arg Gly Val Lys Ile Met Leu Read Ala Gly

275 280 285

Gly Asp Leu Ile Glu Be Met Gly Glu Pro Asp Val Trp Wing Asp Gln

290 295 300

Asp Read His His Ile Read Gly Lys Tyr Gly Cys Read Le Ile Val Glu Arg305 310 315 320

Thr Gly Be Asp Val Arg Be Phe Leu Read His Be Asp Ile Leu Tyr

325 330 335

Glu His Arg Lys Asn Ile Leu Val Ile Lys Gln His Ile Tyr Asn Asp

340 345 350

Ile Be Ser Thr Lys Ile Arg Read Phe Ile Arg Arg Gly Met Ser Val

355 360 365

Gln Tyr Read Leu Pro Asn Ser Val Ile Arg Tyr Ile Gln Gln His Asn

370 375 380

Leu Tyr Gly Asp Be Glu Pro Val Lys Gln Val Met Be Asp Arg Ala385 390 395 400

Asp

<210> 36 <211> 470 <212> PRT

<213> Yarrowia Iipolytica <400> 36

Met Asp Pro Lys Leu Pro Wing Asn Phe Val Arg Pro Be Asp Lys Arg1 5 10 15

Pro Thr Wing Tyr Arg Be Pro Wing Pro Wing Gln Wing Be Read Ile Pro

20 25 30

Asn Ile Wing Pro Ile Gln Pro Tyr Val Leu Glu Asp Ser Gln His Glu

35 40 45

Ile Asp Read Pro Gln Asp Be Pro Arg Read Leu Pro Pro Be Arg Thr Asn

50 55 60

Ser Arg Asp Ser Leu Val Gly Leu Glu Gln Ile Wing Leu Thr Leu Lys65 70 75 80

His His Lys His Asn Pro His Lys Asp His Asn Tyr His Pro Wing

85 90 95

Val Asn Ile Pro Arg Ile His Be Glu Read Asn Met Be Asp Be Ser

100 105 110

Pro Asp Arg Cys Glu Lys Ile Asp Glu Val Asp Leu Glu Val Ser Pro

115 120 125

Asn Glu Ile Gln Thr Asn Phe Be Lys Phe Be Read Gly Asp Gln Wing

130 135 140

Leu Pro Pro Thr Thr Val Glu Glu Met Wing Pro Thr Thr Pro Lys145 150 155 160

Ser Pro Lys Glu Pro Gln Val His Thr Glu Thr Gly Thr Wing Asp Ser

165 170 175

Be Ser Arg Thr Pro Pro Tyr Leu Asp Asn Be Gly Leu Arg Pro

180 185 190

Ile Ile Gln Ser Asp Wing Read Glu Glu Val Pro Ile Gly Val Ser Arg

195 200 205

Gln Ala Arg Asp Read Asp His Tyr Lys Phe Pro Thr His Arg Leu Ser210 215 220Glu Val Met Ile Glu Glu Thr Lys Ser Pro Leu Val Ile Val Wing Cys225 230 235 240

Gly Ser Phe Be Pro Ile Thr Tyr Read His Read Le Arg Met Phe Glu Met

245 250 255

Ala Met Asp Ser Ile Arg Glu Gln Thr Arg Phe Glu Val Ile Gly Gly

260 265 270

Tyr Tyr Be Pro Val Be Asp Asn Tyr Asn Lys Pro Gly Leu Wing Pro

275 280 285

Wing His His Arg Val Arg Met Cys Glu Read Wing Cys Glu Arg Thr Thr

290 295 300

Ser Trp Read Met Val Asp Wing Trp Glu Ser Read Le Gln Pro Thr Tyr Gln305 310 315 320

Arg Thr Wing Thr Val Leu Asp His Phe Asn Glu Glu Ile Asn Ile Lys

325 330 335

Arg Gly Gly Ile Lys Thr Val Be Gly Lys Arg Lys Gly Val Lys Ile

340 345 350

Met Leu Leu Wing Gly Gly Asp Leu Ile Glu Ser Met Gly Glu Pro Asn

355 360 365

Val Trp Glu Glu Arg Asp Read His His Ile Read Gly Arg Tyr Gly Cys

370 375 380

Leu Ile Val Glu Arg Thr Gly Wing Asp Val Arg Ser Phe Leu Leu Ser385 390 395 400

His Asp Ile Met Tyr Glu His Arg Arg Asn Val Leu Val Ile Lys Gln

405 410 415

Leu Ile Tyr Asn Asp Ile Be Being Thr Lys Val Arg Leu Phe Ile Arg

420 425 430

Arg Gly Met Ser Val Gln Tyr Leu Ile Pro Asn Ser Val Ile Arg Tyr

435 440 445

Ile Gln Glu His Arg Read Tyr Val Gly Glu Thr Glu Pro Val Lys Gln

450 455 460

Val Leu Tyr Asp Arg Glu465 470 <210> 37 <211> 365 <212> PRT

<213> Schizosaccharomyces pombe <400> 37

Met Asn Gly Asp Asn Phe Wing Asn Ser Phe Pro Lys Asn Pro Leu Leu1 5 10 15

Being Arg Asn Being Being Being Being Asn Val Ile Gln Tyr Being Asp Glu Phe

20 25 30

Ser Pro Asp Glu Asp Trp Read Asn Glu His Wing Wing Lys Glu His Glu

35 40 45

Glu Arg Ile Arg Arg Pro Ser Val Asn Arg Wing Trp Gln Lys Asn Ser

50 55 60

Thr Be Gly Gly Pro Be Val Be Leu Glu Lys Arg Glu Wing Asp Val65 70 75 80

Wing To Be Read Gly Glu Val Met Asp Read Glu Glu Val Pro Arg Gly Ile

85 90 95

Thr Arg Gln Wing Arg Gln Read Asn Glu Tyr Ile Phe Pro Lys His Arg

100 105 110

Phe Arg Asn His Leu Val Asp Glu Gly Lys Ile Pro Leu Val Leu Val

115 120 125

Cys Wing Gly Be Phe Be Pro Ile Thr Tyr Read His Read Arg Met Phe

130 135 140

Glu Met Wing As Thr Thr Ile Gln Glu Gln Thr Asn Met Glu Leu Val145 150 155 160

Gly Wing Tyr Phe Ser Pro Val Asn Asp His Tyr Lys Lys Glu Gly Leu

165. 170 175

Pro Wing Ala Tyr His Arg Val Arg Met Cys Glu Read Ala Cys Glu Arg

180 185 190

Thr Be Ser Trp Leu Met Val Asp Wing Trp Glu Ser Leu Gln Pro Ser

195 200 205

Tyr Thr Cys Thr Wing Arg Val Leu Asp His Phe Asp Glu Glu Ile Asn210 215 220

Gln Lys Arg Gly Gly Ile Thr Read Asp Gly Thr Lys Arg Pro Cys225 230 235 240

Lys Ile Met Leu Leu Wing Gly Gly Asp Leu Ile Wing Be Met Gly Glu

245 250 255

Pro Gly Val Trp Being Asp Lys Asp Read His His Ile Read Gly Lys Phe

260 265 270

Gly Cys Cys Ile Val Glu Arg Thr Gly Ser Asp Val Trp Wing Phe Leu

275 280 285

Leu Ala His Asp Ile Met Phe Ala Tyr Arg Gly Asn Ile Leu Val Ile

290 295 300

Lys Gln Read Ile Tyr Asn Asp Ile Be Being Thr Lys Val Arg Read Le Phe305 310 315 320

Ile Arg Arg Gly Met Ser Ile Arg Tyr Read Leu Pro Asn Ser Val Ile

325 330 335

Gln Tyr Ile Glu Arg Tyr Wing Read Tyr Arg Wing Asp Wing Glu Pro Val Lys

340 345 350

Thr Ile Phe Tyr Gln Pro Phe Val Arg Met Glu Pro355 360 365

<210> 38 <211> 584 <212> PRT

<213> Ustilago maydis <400> 38

Met Pro Arg Met Thr Asp Ser Gn Wing Gly Pro Gly Gly Pro Ser 1 5 10 15

Leu Lys Phe Ala Wing Leu Ser Val Gln Ser Phe His Leu Asp Ser Pro

20 25 30

Pro Phe Thr Gly Wing Val Gly Wing Gly Wing Gly Pro Read Asp Glu Wing

35 40 45

Gly Lys Arg Leu Wing Be His Asp Wing Asp Pro Wing Tyr His Be Leu50 55 60Asp Be Gly Tyr Be Gln His Gln Pro Gln Be Pro Cys Wing Asp65 70 75 80

Arg Gln Be Arg Arg Gly Gly Gly Ser Be Pro Read Be Lys Ala Ile

85 90 95

Be Val Thr Be Ser Gly Glu Asp His Asp Pro Tyr Arg Arg Be Met

100 105 110

Be Gln Thr Arg Pro Pro Be Gln Be Gln Thr Read Arg Be Gln Asp

115 120 125

Arg Gln His Asn Thr Asp Val Lys Ser Be Met Wing Gln Pro Gln Ser

130 135 140

Leu Pro Pro Asn Gly Met Thr Thr Be Glu Asp Wing Pro Be Wing Ser145 150 155 160

Asn His Be Asp Pro His Arg Be Asn Asp Be Asn Gly His Leu Glu

165 170 175

Ser Arg Phe Asp Arg Asp Val Pro His Trp Asp Leu Asp Glu Lys Leu

180 185 190

Ser Be Arg Gln Ala Ser Be Ala Ser Be Ala Thr Alu Thr Be Pro Asp

195 200 205

Lys Pro Ile Met Arg Read Be Be Thr Glu Thr Be Be Wing Val Asp

210 215 220

Arg Pro Gly Thr Arg Arg Be Asp Asp Phe Gln Thr Gly Be Asn Arg225 230 235 240

Glu Thr Read Ile Wing Pro Asn Pro Be Be His Arg Thr Asn Be Asn

245 250 255

Be Thr Be Asn Be Arg Thr Read Be Phe Be Wing Val Leu Asp Glu

260 265 270

Arg Pro Be Tyr Be Gln Phe Be Glu Asp Read Asp Wing Gln Glu Asp

275 280 285

Wing Wing Ser Be Wing Wing Asp Arg Asp Glu Glu Val Asp Gly Pro Glu

290 295 300

Gly Leu Be Glu Wing Phe Arg Leu Arg Asp Arg Arg Val Pro Gln Gln305 310 315 320Gly Gln Thr Gln Glu Asp Tyr Be Phe Pro Arg His Arg Leu Pro Thr

325 330 335

Arg Met Arg Asp Glu Ser Lys Thr Pro Read Val Val Val Val Cys Gly Wing

340 345 350

Be Phe Be Pro Pro Thr Tyr Read His Met Arg Ile Phe Glu Met Wing

355 360 365

Lys Asp Gln Ile Ile Glu Ser Gly Lys Tyr Glu Read Leu Wing Gly Tyr

370 375 380

Tyr Be Pro Val Be Asp Tyr Tyr Lys Lys Glu Gly Leu Wing Lys Wing385 390 395 400

Thr His Arg Val Arg Met Cys Glu Leu Wing Val Glu Lys Thr Be Thr

405 410 415

Trp Read Met Val Asp Wing Trp Glu Be Read Gln Asp Glu Tyr Gln Arg

420 425 430

Thr Wing Val Val Leu Asp His Phe His Asp Glu Ile Asn Gly Ser Ser

435 440 445

Asn Gly Gly Val Leu Read Gly Asp Gly Thr Arg Lys Asn Val Lys Ile

450 455 460

Met Leu Leu Wing Gly Gly Asp Leu Ile Gln Ser Met Gly Glu Pro Gly465 470 475 480

Val Trp Wing Thr Wing Asp Read His His Ile Read Gly Gln Tyr Gly Cys

485 490 495

Leu Ile Val Glu Arg Thr Gly Wing Asp Val Trp Ser Phe Leu Leu Ser

500 505 510

His Asp Leu Leu Trp Lys Tyr Arg Arg Asn Leu Lys Ile Val Lys Gln

515 520 525

Thr Ile Tyr Asn Asp Ile Being Being Being Lys Ile Arg Read Phe Val Arg

530 535 540

Arg Gly Gln Ser Ile Lys Tyr Read Leu Pro Asn Ser Val Ile Gln Tyr545 550 555 560

Ile Glu Lys Glu Gly Leu Tyr Arg Leu Pro Pro Asp Glu Asp Leu Lys565 570 575Ser Asp Ser Glu His Wing Asn Trp580

<210> 39 <211> 537 <212> PRT

<213> Cryptococcus neoformans <400> 39

Met Thr Arg Val Ile Leu Be Leu Leu Be Leu Be Leu Thr Leu1 5 10 15

Read Ser Pro Phe Gly Gly Thr Wing Thr Be Glu Met Pro Asp Val

20 25 30

Pro Be Read Be Read Glu Pro Be Gln His Be Arg Be Pro Val Be Read

35 40 45

Thr Asn Pro Be Wing Arg Gln Gln Gly Pro Wing Arg Thr Wing Be Asn

50 55 60

Thr Be Gln Thr Ile Be Asn Pro Glu Pro Val Be Gly Thr Arg65 70 75 80

Glu Thr Pro Be Lys Wing Ile Be Be Read Lys Be Ile Gln Pro Pro

85 90 95

Thr Pro Pro Val Pro Ile Thr Be Asp Asp Glu Read Be Ser Thr Arg

100 105 110

Be Arg Arg Read Ile Be Gly Tyr Read Phe Gly Asn Pro Pro Be Pro

115 120 125

Thr Cys Thr Gly Pro Glu Thr Be Pro Val Thr Wing Ward Be His Thr

130 135 140

Be Thr Wing Glu Glu Be Arg Asp Pro Met Met Be Arg Lys Phe Ser145 150 155 160

Pro Thr Leu Glu Lys Wing Arg Asp Val Glu Asn Arg Met Wing Glu Glu

165 170 175

Read Asp Be Pro Pro Read Thr Asp Asp Be Gln Ile Asp Pro Arg Asp

180 185 190

Wing Leu -Glu Met Asp Be Thr Pro Pro Pro Pro Be Leu Glu Ser Pro195 200 205

Lys Pro Wing Asn Be Asp Leu Val Be Met Asn Glu Pro Read Le Be

210 215 220

Asp Val Gly Wing Gly Glu Wing Lys Val Lys Gly Arg Lys Be Glu Arg225 230 235 240

Wing Arg Lys Arg Glu Thr Gly Tyr Asp Wing Leu Glu Gly Arg Asn Asp

245 250 255

Gly Glu Wing Asp Read Asp Read Asn Read Asn Wing Pro Gln Wing Glu Gly Gly Gly

260 265 270

Ser Gly Wing Tyr Leu Wing Gly Lys Wing Glu Tyr Arg Phe Pro Arg His

275 280 285

Arg Leu Arg Thr Lys Met His Asp Glu Asn Lys Ile Pro Leu Val Ile

290 295 300

Val Cys Wing Gly Be Phe Pro Pro Thr Be Tyr Read His Read Arg Met305 310 315 320

Phe Glu Met Wing Lys Asp Glu Ile Val Glu Ser Gln Thr Tyr Glu Ile

325 330 335

Met Wing Gly Tyr Tyr Be Pro Val Ser Be Tyr Tyr Lys Lys Be Gly

340 345 350

Leu Pro Wing Pro Pro His Arg Val Arg Met Cys Glu Leu Wing Pro Val Glu

355 360 365

His Thr Be Thr Trp Leu Met Val Asp Pro Trp Glu Wing Gly Gln Pro

370 375 380

Glu Tyr Gln Arg Thr Wing Phe Val Leu Asp His Phe Asp Glu Met Leu385 390 395 400

Asn Gly Gly Glu His Gly Lys Gly Gly Read Val Met Arg Asp Gly Thr

405 410 415

Arg Arg Arg Tyr Lys Ile Met Leu Read Leu Wing Gly Gly Asp Leu Ile Glu

420 425 430

Being Phe Gly Glu Pro Gly Val Trp Being Glu Pro Asp Read His Val Ile

435 440 445

Read Gly Arg Phe Gly Cys Read Ile Val Glu Arg Wing Gly Ser Asp Val450 455 460

Trp Ala Phe Leu Read His Asp Ile Leu Tyr His His Arg Arg Asn465 470 475 480

Val Val Val Ile Lys Gln Leu Ile Tyr Asn Asp Ile Ser Be Thr Lys

485 490 495

Val Arg Leu Phe Val Arg Arg Gly Met Ser Ile Lys Tyr Leu Leu Pro

500 505 510

Asn Ser Val Ile Gln Tyr Ile Gln Asp Asn Lys Leu Tyr His Gly Ser

515 520 525

Asp Pro Lys Gly Met

530 535

<210> 40 <211> 287 <212> PRT

<213> Aspergillus oryzae <400> 40

Met Thr Asp Ile Thr Gly Gly His Gly Glu Asp Val His Gln Pro Pro 1 5 10 15

His Ile Pro Wing Pro Met Wing Glu Asp Tyr Gln Phe Pro Glu Leu

20 25 30

Arg Leu Lys Arg Lys Met Asp Asp Pro Glu Lys Thr Pro Leu Leu Leu

35 40 45

Val Cys Wing Gly Be Phe Be Pro Ile Thr Tyr He Read His He Read Arg Met

50 55 60

Phe Glu Met Wing Asp Wing Tyr Val Lys Phe Ser Ser Asn Phe Glu Leu65 70 75 80

Ile Gly Gly Tyr Leu Be Pro Val Ser Asp Wing Tyr Arg Lys Wing Gly

85 90 95

Leu Wing Wing Wing Glu His Arg Val Wing Met Cys Gln Leu Wing Val Glu Wing

100 105 110

Gln Thr Be Asp Trp Leu Met Val Val Asp Thr Trp Glu Pro Met -Gln Lys115 120 125Ala Tyr Gln Pro Thr Val Val Leu Asp His Phe Asp His Glu Ile

130 135 140

Asn Thr Val Arg Glu Gly Ile Glu Wing Wing Asp Gly Thr Arg Lys His145 150 155 160

Val Arg Ile Wing Leu Leu Wing Gly Wing Asp Leu Ile His Thr Met Ser

165 170 175

Thr Pro Gly Val Trp Being Glu Lys Asp Read Asp His Ile Read Gly Lys

180 185 190

Tyr Gly Be Phe Ile Val Glu Arg Be Gly Thr Asp Ile Asp Glu Wing

195 200 205

Leu Wing Wing Leu Gln Pro Trp Lys Asp Asn Ile His Val Ile Gln

210 215 220

Leu Ile Gln Asn Asp Val Ser Be Thr Lys Ile Arg Leu Phe Leu Arg225 230 235 240

Arg Glu Met Ser Val Arg Tyr Leu Ile Pro Val Pro Val Ile Arg Tyr

245 250 255

Ile Glu Gln His Arg Read Tyr Gly Asp Asp Asn Thr Thr Wing Asn Ser

260 265 270

Thr Be Asp Lys Gly Lys Gly Lys Gln Glu Pro Be Lys Be Gly

275 280 285

<210> 41 <211> 288 <212> PRT

<213> Aspergillus fumigatus <400> 41

Met Thr Asp Met Val Gly Gly Gln Ser Asp Asp Val His Gln Val Pro1 5 10 15

Pro Pro Read Pro Pro Wing Pro Met Glu Asn Tyr Thr Phe Pro Glu His

20 25 30

Arg Leu Lys Arg Lys Met Asp Asp Pro Glu Lys Thr Pro Leu Leu Leu

35 40 45

Val Cys Wing Gly Be Phe Be Pro Ile Thr Tyr LeU His Leu Arg Met50 55 60

Phe Glu Met Wing Asp Wing Tyr Val Lys Phe Ser Thr Asp Phe Glu Leu65 70 75 80

Ile Gly Gly Tyr Leu Be Pro Val Ser Asp Wing Tyr Arg Lys Wing Gly

85 90 95

Leu Wing Be Wing Glu His Arg Val Wing Met Cys Gln Leu Wing Val Val Asp

100 105 110

Gln Thr Be Asn Trp Read Met Val Asp Thr Trp Glu Pro Met Gln Lys

115 120 125

Glu Tyr Gln Pro Thr Val Val Valu Leu Asp His Phe Asp Tyr Glu Ile

130 135 140

Asn Val Val Arg Glu Gly Ile Asp Gly Wing Asn Gly Thr Arg Lys Pro145 150 155 160

Val Arg Val Wing Leu Leu Wing Gly Wing Asp Leu Ile His Thr Met Ser

165 170 175

Thr Pro Gly Val Trp Being Glu Lys Asp Read Asp His Ile Read Gly Lys

180 185 190

Tyr Gly Be Phe Ile Val Glu Arg Be Gly Thr Asp Ile Asp Glu Wing

195 200 205

Leu Wing Wing Leu Gln Pro Trp Lys Asp Asn Ile Tyr Val Ile Gln

210 215 220

Leu Ile Gln Asn Asp Val Ser Be Thr Lys Ile Arg Leu Phe Leu Arg225 230 235 240

Arg Glu Met Ser Val Arg Tyr Leu Ile Pro Val Pro Val Ile His Tyr

245 250 255

Ile Glu Gln His His Leu Tyr Glu Asp Asp Ser Thr Thr Thr Wing

260 265 270

Be Ser Asa Wing Asp Lys Gly Lys Glu Pro Be Gln Pro Gly Lys Be Gly

275 280 285

<210> 42 <211> 285 <212> PRT <213> Aspergillus nidulans <400> 42

Met Thr Glu Be Thr Gln Glu Gln Gn Asn Asp Gly Gln Arg Met Pro1 5 10 15

Pro Wing Pro Wing Pro Thr Val Glu Asp Tyr Val Phe

20 25 30

Leu Lys Arg Val Met Asp Asp Pro Glu Lys Thr Pro Leu Leu Leu Ile

35 40 45

Cys Wing Gly Be Phe Be Pro Ile Thr Phe Read His Read Le Arg Met Phe

50 55 60

Glu Met Wing Asp Wing Tyr Val Lys Read Thr Thr Asp Phe Glu Ile Ile65 70 75 80

Gly Gly Tyr Leu Be Pro Val Be Asp Wing Tyr Arg Lys Wing Gly Leu

85 90 95

Wing Be Wing Asn His Arg Ile Wing Met Cys Gln Arg Wing Val Asp Gln

100 105 110

Thr Be Asp Trp Met Met Val Asp Thr Trp Glu Pro Met His Lys Glu

115 120 125

Tyr Gln Pro Thr Ile Val Valu Asu His Phe Asp Tyr Glu Ile Asn

130 135 140

Thr Arg Lys Gly Ile Asp Thr Thr Gly Lys Gly Thr Thr Lys Arg Val145 150 155 160

Gln Val Val Leu Leu Wing Gly Wing Asp Leu Val His Thr Met Ser Thr

165 170 175

Pro Gly Val Trp Being Glu Lys Asp Read Asp His Ile Read Gly Gln Tyr

180 185 190

Gly Thr Phe Ile Val Glu Arg Be Gly Thr Asp Ile Asp Glu Ala Leu

195 200 205

Wing Wing Leu Gln Pro Trp Lys Lys Asn Ile His Val Ile Gln Gln Leu

210 215 220

Ile Gln Asn Asp Val Ser Be Thr Lys Ile Arg Leu Phe Leu Arg Arg225 230 235 240Asp Met Ser Val Arg Tyr Leu Ile Pro Asp Pro Val Ile

245 250 255

Tyr Glu Asn Asn Leu Tyr Met Asp Asp Gly Thr Thr

260 265 270

Asp Lys Gly Lys Thr Arg Glu Glu Pro Wing Pro Ser Asn275 280 285

<210> 43 <211> 276 <212> PRT

<213> Giberella zeae <400> 43

Met Cys Wing Asp Tyr Gln Tyr Val Lys Glu His Thr Arg Glu Asp Tyr15 10 15

Val Phe Pro Be His Arg Phe Gln Arg Be Cys Be Pro Asp Lys Thr

20 25 30

Pro Leu Cys Leu Val Cys Wing Gly Ser Phe Ser Pro Ile Thr Phe Leu

35 40 45

His Leu Arg Met Phe Pro Met Arg Wing Asp His Wing Arg Asn Glu Asp

50 55 60

Phe Glu Val Val Wing Gly Val Leu Ser Pro Val Ser Asp Wing Tyr Lys65 70 75 80

Lys Lys Gly Leu Wing Pro Wing His His Arg Ile Glu Met Cys Arg Leu

85 90 95

Wing Thr Glu Asn Thr Be Lys Trp Read Met Val Val Asp Pro Trp Glu Wing

100 105 110

Glu Ser Pro Thr Tyr Ile Pro Thr Lys Val Leu Asp His Phe Asp

115 120 125

Tyr Glu Ile Asn Glu Val Met Gly Gly Val Glu Cys Thr Asp Gly Thr

130 135 140

Arg Lys Arg Cys Arg Ile Val Leu Leu Wing Gly Leu Asp Leu Ile Gln145 150 155 160

Thr Met Ser Thr Pro Gly Val Trp Asp Glu Arg Asp Read Asp His Ile165 170 175

Read Gly Asn Tyr Gly Val Phe Wing Read Le Glu Arg Thr Gly Thr Glu Ile

180 185 190

Asp Ser Thr Leu Wing Asn Leu Lys Gln Trp Glu Lys Asn Ile His Ile

195 200 205

Ile Arg Gln Val Val Asn Asp Ile Be Being Thr Lys Ile Arg Leu

210 215 220

Leu Leu Lys Arg Asn Met Ser Ile Asp Phe Leu Ile Pro Asp Asp Val225 230 235 240

Ile Ser Tyr Ile Tyr Glu His Asn Read Tyr Arg Asp Read Asp Met Pro

245 250 255

Asp Ser Lys Gly Lys Glu Lys Wing Read Thr Asn Gly Pro Asp Wing Gly

260 265 270

Thr Ser Thr Gly

275 <210> 44 <211> 317 <212> PRT

<213> Neurospora crassa <400> 44

Met Be Ser Gln Thr Be Thr Gly Met Wing Thr Pro Val Thr Tyr Pro1 5 10 15

Pro Pro Glu Gln Wing Be Thr Gly Asn Thr Thr Val Pro Tyr Thr Phe

20 25 30

Pro Gln Wing Lys Read Lys Read Gln Gln Thr Gln Pro

35 40 45

Leu Val Leu Val Cys Wing Gly Ser Phe Ser Pro Ile Thr Phe Leu His

50 55 60

Leu Arg Met Phe Glu Met Wing Be Asp Phe Val Arg Phe Asn Thr Asn65 70 75 80

Phe Glu Val Cys Gly Gly Tyr Leu Be Pro Val Ser Asp Wing Tyr LysS5 90 95Lys Gly Wing Gly Leu Pro Wing Gly His His Arg Val Glu Met Cys Ser Arg

100 105 110

Glu Val Wing His Ser Ser Trp Leu Met Val Asp Pro Phe Glu Thr Val

115 120 125

Asn Cys Asp Glu Asn Gly Glu Pro Wing Tyr Val Pro Thr Wing Arg Val

130 135 140

Read His Arg Phe Asp His Glu Ile Asn Thr Val Read Gly Gly Ile Glu145 150 155 160

Gly Thr Asp Gly Val Arg Arg Lys Wing Lys Ile Wing Read Leu Wing Gly

165 170 175

Wing Asp Leu Val Met Be Met Gly Glu Pro Gly Leu Trp Be Met Val

180 185 190

Asp Leu Gly Val Ile Leu Gly Glu Tyr Gly Wing Phe Ile Ile Glu Arg

195 200 205

Ser Gly Thr Asp Ile Asp Glu Wing Leu Wing Wing Thr Leu Arg Gln Tyr Glu

210 215 220

Asp Asn Ile Trp Val Ile Ser Gln Val Ile Gln Asn Asp Ile Ser Ser225 230 235 240

Thr Lys Val Arg Leu Phe Leu Lys Lys Asp Leu Ser Val Arg Tyr Leu

245 250 255

Ile Pro Asp Pro Val Val Glu Tyr Ile Glu Glu His Gly Leu Phe Gln

260 265 270

Asp Glu Gln To Be Lys Lys Asn Asn Asn Asp To Be Being Thr Gly

275 280 285

Gly Lys Asp Lys Glu Lys Glu Lys Pro Thr

290 295 300

Thr Pro Be Ser Be Thr Glu Glu Thr Thr Gln Gln Ser305 310 315

<210> 45 <211> 292 <212> PRT

<213> Magnaporthe grisea <400> 45

Met Ser Asn Wing Thr Be Pro Thr Pro Gln Phe Pro Pro Thr Thr Pro1 5 10 15

Gly Tyr Thr Phe Pro Val Asp Lys Read Arg Thr Arg Gln Thr Gln Asn

20 25 30

Glu Arg Thr Pro Leu Val Leu Val Wing Cys Gly Ser Phe Ser Pro Ile

35 40 45

Thr Tyr Leu His Leu Arg Met Phe Glu Met Wing Arg Wing Gly Pro Wing

50 55 60

Asp Asn Arg Thr Gly Asp His Cys Being Read Asn Thr Asn Phe Glu Val65 70 75 80

Val Gly Gly Tyr Ile Be Pro Val Ser Asp Wing Tyr Lys Lys Wing Gly

85 90 95

Leu Wing Pro Wing His His Ile Asn Met Cys Lys Leu Being Leu Wing

100 105 110

Ser Ser Ser Tr Ile Met Val Asp Glu Tyr Glu Thr Ser Val Val Asn

115 120 125

Pro Thr Thr Asn Glu Pro Wing Tyr Thr Pro Thr Wing Gln Val Leu Wing

130 135 140

Lys Leu Asp His Glu Ile Asn Thr Val Leu Gly Gly Ile Gln Ser Ala145 150 155 160

Asp Asp Pro Asn Lys Arg Thr Arg Wing Arg Ile Cys Read Leu Wing Gly

165 170 175

Gly Asp Leu Val Leu Thr Met Be Thr Pro Gly Asp Leu Trp Wing Pro Be

180 185 190

Asp Leu Asp Val Ile Leu Gly Pro Lys Phe Gly Wing Phe Ile Val Glu

195 200 205

Arg Be Gly Thr Asp Thr Glu Glu Wing Read Wing Be Read Gln Arg Tyr

210 215 220

Lys Asp Asn Ile Trp Val Ile Pro Gln Val Ile Gln Asn Asp Val Ser225 230 235 240

Ser Thr Lys Ile Arg Leu Phe Leu Lys Lys Asn Leu Ser Ile Arg Tyr245 250 255

Leu Ile Pro Asp Pro Val Val Arg Tyr Ile Glu Glu His Gly Leu Phe

260 265 270

Asn Gly Glu Phe Be Gly Wing Be Ser Be Lys Glu Wing Glu Pro Be

275 280 285

Pro Ser Thr Wing

290 <210> 46 <211> 120 <212> PRT

<213> Ustilago maydis <400> 46

Met Leu Leu Wing Gly Gly Asp Leu Ile Gln Ser Met Gly Glu Pro Gly1 5 10 15

Val Trp Wing Thr Wing Asp Read His His Ile Read Gly Gln Tyr Gly Cys

20 25 30

Leu Ile Val Glu Arg Thr Gly Wing Asp Val Trp Ser Phe Leu Leu Ser

35 40 45

His Asp Leu Leu Trp Lys Tyr Arg Arg Asn Leu Lys Ile Val Lys Gln

50 55 60

Thr Ile Tyr Asn Asp Ile Being Being Being Lys Ile Arg Leu Phe Val Arg65 70 75 80

Arg Gly Gln Ser Ile Lys Tyr Read Leu Pro Asn Ser Val Ile Gln Tyr

85 90 95

Ile Glu Lys Glu Gly Leu Tyr Arg Leu Pro Pro Asp Glu Asp Leu Lys

100 105 110

Ser Asp Ser Glu His Wing Asn Trp115 120

<210> 47 <211> 225 <212> PRT

<213> Chaetomium globosum <400> 47

Met Thr Wing Gly Thr Ile Asn Be Asp Thr Gln Thr Pro Thr Met1 5 10 15

Gly Pro Wing Val Asp Gly Pro Wing Gln Pro Wing Tyr Asn Phe Pro Thr Gln

20 25 30

Lys Leu Lys Arg Gln Met Thr Gln Pro Gly Lys Thr Pro Leu Val Leu

35 40 45

Val Cys Wing Gly Be Phe Be Pro Ile Thr Tyr He Read His He Read Arg Met

50 55 60

Phe Glu Met Gly Asp Wing Phe Val Arg Phe Asn Thr Asp Phe Glu Val65 70 75 80

Cys Wing Gly Tyr Leu Be Pro Val Ser Asp Wing Tyr Lys Lys Val Gly

85 90 95

Read Wing Pro Gly Be His Arg Val Asn Met Cys Gly Arg Wing Val GlU

100 105 110

Gln Ser Pro Trp Read Met Val Asp Pro Phe Glu Thr Val Asn Cys Asp

115 120 125

Glu Asn Gly Glu Pro Gln Tyr Val Pro Thr Lys Wing Val Leu Arg His

130 135 140

Phe Asp His Glu Ile Asn Thr Val Leu Gly Gly Ile Glu Pro Wing Asp145 150 155 160

Gly Gln Met Lys Lys Wing Arg Ile Wing Leu Leu Wing Gly Wing Asp Leu

165 170 175

Val Met Being Met Gly Gly Phe Asp Thr Gly Being Arg Val Being Asn Val

180 185 190

Lys Leu Thr Cys Thr Arg Gly Wing Trp Wing Read Gly Ser Lys Gly Ser

195 200 205

Gly His Asp Pro Arg Arg Val Arg Arg Phe His His Arg Wing Leu Gly210 215 220

Asp225

<210> 48 <211> 426 <212> PRT

<213> Saccharomyces cerevisae <400> 48

Met Be Glu Pro Val Ile Lys Be Read Leu Asp Thr Asp Met Tyr Lys1 5 10 15

Ile Thr Met His Wing Wing Val Phe Thr Asn Phe Pro Asp Val Thr Val

20 25 30

Thr Tyr Lys Tyr Thr Asn Arg Be Ser Gln Leu Thr Phe Asn Lys Glu

35 40 45

Wing Ile Asn Trp Leu Lys Glu Gln Phe Ser Tyr Leu Gly Asn Leu Arg

50 55 60

Phe Thr Glu Glu Glu Ile Glu Tyr Leu Lys Gln Glu Ile Pro Tyr Leu65 70 75 80

To Be Ala Tyr Ile Lys Tyr Ile To Be Ser Asn Tyr Lys Read His

85 90 95

Pro Glu Glu Gln Ile Be Phe Thr Be Glu Glu Ile Glu Gly Lys Pro

100 105 110

Thr His Tyr Lys Leu Lys Ile Leu Val Ser Gly Ser Trp Lys Asp Thr

115 120 125

Ile Leu Met Arg Be Leu Thr Val Leu Ile Be Glu Wing Tyr Leu Ile

130 135 140

Val Thr Be Thr Gly Leu Arg Asn His Arg Gln Wing Glu Lys Lys Wing145 150 155 160

Glu Thr Read Phe Asp Asn Gly Ile Arg Phe Arg Ile His Gly Thr Arg

165 170 175

Arg Arg Arg Ser Read Lys Wing Gln Asp Read Ile Met Gln Gly Ile Met

180 185 190

Lys Wing Val Asn Gly Asn Pro Asp Arg Asn Lys Be Read Leu Read Le Gly

195 200 205

Thr Be Asn Ile Leu Phe Wing Lys Lys Tyr Gly Val Lys Pro Ile Gly210 215 220Thr Val Wing His Glu Trp Val Met Gly Val Wing Ile Ser Glu Leu225 230 235 240

Asp Tyr Read His Wing Asn Lys Asn Wing Met Asp Cys Trp Ile Asn Thr

245 250 255

Phe Gly Wing Lys Asn Wing Gly Wing Wing Wing Wing Wing Thr Thr

260 265 270

Asp Asp Phe Lys Be Phe Arg Pro Pro Tyr Be Asp Wing Tyr Val

275 280 285

Gly Val Arg Gln Asp Being Gly Asp Pro Val Glu Tyr Thr Lys Lys Ile

290 295 300

Be His His Tyr His Asp Val Leu Lys Leu Pro Lys Phe Ser Lys Ile305 310 315 320

Ile Cys Tyr Ser Asp Ser Read Asn Val Glu Lys Wing Ile Thr Tyr Ser

325 330 335

His Wing Wing Lys Glu Asn Gly Met Leu Wing Thr Phe Gly Ile Gly Thr

340 345 350

Asn Phe Thr Asn Asp Phe Arg Lys Lys Be Glu Pro Gln Val Lys Be

355 360 365

Glu Pro Leu Asn Ile Val Ile Lys Leu Leu Glu Val Asn Gly Asn His

370 375 380

Alle Ile Lys Ile Ser Asp Asn Leu Gly Lys Asn Met Gly Asp Pro Ala385 390 395 400

Thr Val Lys Arg Val Lys Glu Glu Read Gly Tyr Thr Glu Arg Ser Trp

405 410 415

Ser Gly Asp Asn Glu Wing His Arg Trp Thr420 425

<210> 49 <211> 431 <212> PRT

<213> Candida glabrata <400> 49

Met Ser Be Met Thr Pro Wing Ile Thr Be Read Le Asp Thr Asp Met1 5 10 15

Tyr Lys Ile Thr Met His Wing Val Val Phe Thr Asn Phe Pro Asp Wing

20 25 30

Arg Val Val Tyr Lys Phe Thr Asn Arg Thr Wing Gln Phe His Phe Asn

35 40 45

Lys Arg Wing Val Asp Trp Ile Lys Glu Gln Phe Arg Read Leu Gly Asp

50 55 60

Leu Thr Phe Thr His Asp Asp Val Glu Tyr Leu Wing Arg Glu Ile Pro65 70 75 80

Phe Leu Pro Alys Lys Tyr Leu His Tyr Ile Glu Asn Gly Phe Thr Leu

85 90 95

Lys Pro Asp Glu Gln Ile Glu Read Asp Cys Gln Glu Ile Lys Gly Lys

100 105 110

Glu Asp Gln Tyr Asp Read His Ile Read Val Lys Gly Read Trp Ile Asp

115 120 125

Thr Ile Leu Tyr Glu Ile Pro Ile Leu Wing Leu Leu Ser Glu Wing Tyr

130 135 140

Phe Lys Phe Val Asp Thr Asp Trp Asp Tyr Glu Asn Gln Read Ser Asn145 150 155 160

Wing Lys Glu Lys Wing Leu Arg Leu Phe Glu Asn Asn Leu Ser Phe Ser

165 170 175

Glu Phe Gly Thr Arg Arg Arg Arg Be Phe Lys Thr Gln Asp Leu Val

180 185 190

Met Glu Gly Ile Met Gln Wing Val Lys Glu Asn Pro Glu Lys Tyr Arg

195 200 205

Pro Leu Leu Leu Gly Thr Be Asn Ile Leu Phe Ala Lys Lys Tyr Gly

210 215 220

Val Lys Pro Ile Gly Thr Val Wing His Glu Trp Ile Met Gly Ile Wing225 230 235 240

Ser Ile Thr Gly Asp Tyr Pro Glu Thr Asn Arg Ile Ala Met Asp Tyr

245 250 255

Trp Ile Lys Thr Phe Gly Lys Glu His Wing Gly Leu Wing Leu Thr Asp260 265 270

Thr Phe Gly Thr Asp Asp Phe Read Lys Ser Phe Lys Pro Pro Tyr Ser

275 280 285

Asp Tyr Tyr Ile Gly Val Arg Gln Asp Ser Gly Asp Pro Ile Lys Tyr

290 295 300

Thr Glu Lys Ile Wing His His Phe His Asp Val Leu Lys Leu Pro Lys305 310 315 320

Phe Ser Lys Phe Ile Cys Tyr Be Asp Be Read Asn Ile Asp Lys Wing

325 330 335

Ile Glu Tyr Gly Lys Val Wing Glu Wing His Gly Ile Lys Ser Thr Phe

340 345 350

Gly Ile Gly Thr Asn Phe Thr Asn Asp Phe His Lys Be Asp Pro

355 360 365

Ser Val Lys Ser Wing Pro Leu Asn Ile Val Ile Lys Leu Leu Glu Val

370 375 380

Asn Gly Asn His Be Ile Lys Ile Be Asp Asn Wing Gly Lys Asn Met385 390 395 400

Gly Asp Pro Asp Thr Val Lys Val Lys Val Lys Glu Glu Leu Gly Tyr Val

405 410 415

Glu Arg Gln Trp Wing Gly Gly Asn Glu Wing His Arg Be Wing Wing420 425 430

<210> 50 <211> 427 <212> PRT

<213> Kluyveromyces lactis <400> 50

Met Glu Pro Val Ile Thr Be Phe Leu Asp Thr Asp Met Tyr Lys Leu1 5 10 15

Thr Met His Wing Val Tyr Wing Thr His Phe Lys Asp Val Lys Val Lys

20 25 30

Tyr Lys Tyr Thr Asn Arg Be Pro Gln Met Thr Phe Asn Lys Glu Ala35 40 45Val Glu Trp Read Lys Gly Gln Phe Leu Read Leu Wing Asn Ile Arg Leu

50 55 60

Thr Thr Glu Glu Leu Lys Tyr Ile Lys Glu Wing Ile Pro Tyr Leu Pro65 70 75 80

Glu Wing Tyr Leu Glu Phe Ile Ser Asn Gly Gly Phe Glu Val Asn Pro

85 90 95

Lys Asp Glu Ile Lys Phe Glu Ile Arg Glu Ile Glu Gly Glu Pro Gly

100 105 110

His Tyr Glu Leu Asp Ile Ser Ile Glu Gly Leu Trp Ile Lys Thr Ile

115 120 125

Trp Tyr Glu Ile Pro Val Leu Wing Leu Val Ser Glu Wing Tyr Phe Lys

130 135 140

Phe Val Asp Thr Asp Trp Thr Asp Gly Gln Val Glu Lys Wing Tyr145 150 155 160

Lys Wing Lys Wing Lys Leu Read Asp His Asp Ile Val Phe Ser Glu Phe

165 170 175

Gly Thr Arg Arg Arg Arg Be Phe Lys Thr Gln Asp Read Val Met Gln

180 185 190

Gly Ile Ile Asn Wing Wing Lys Asp Cys Asp Lys Ser Lys Tyr Val Leu

195 200 205

Gly Thr Ser Asn Val Leu Phe Alys Lys Tyr Asn Val Asn Pro Ile

210 215 220

Gly Thr Val Wing His Glu Trp Met Met Gly Ile Wing Ser Ile Thr Asn225 230 235 240

Asp Tyr Leu Asn Wing Asn Lys Asn Wing Met Asp Tyr Trp Ile Glu Thr

245 250 255

Phe Gly Met Glu Asn Wing Gly Leu Wing Wing Leu Thr Asp Thr

260 265 270

Asp Be Phe Read Lys Be Phe Tyr Pro Pro Tyr Be Asp Wing Tyr Ile

275 280 285

Gly Val Arg Gln Asp Be Gly Asp Pro Ile Leu Tyr Thr Glu Lys Ile290 295 300Ala His His Tyr Asn Asp Val Leu Lys Leu Pro Lys Phe Ser Lys Ile305 310 315 320

Ile Cys Tyr Be Asp Be Read Asn Pro Asp Arg Wing Ile Glu Tyr Wing

325 330 335

Gln Val Wing His Lys His Gly Leu Lys Wing Thr Phe Gly Ile Gly Thr

340 345 350

Asn Phe Thr Asn Asp Phe Gln Arg Lys Ser Asp Val Ser Val Lys Ser

355 360 365

Glu Pro Leu Asn Ile Val Ile Lys Leu Leu Glu Val Asn Gly Asn His

370 375 380

Wing Ile Lys Ile Ser Asp Asn Read Gly Lys Asn Met Gly Asp Pro Glu385 390 395 400

Thr Val Arg Arg Val Lys Glu Glu Leu Gly Tyr

405 410 415

Wing Gly Asp Asn Glu Wing His Arg Trp Wing Wing420 425

<210> 51 <211> 430 <212> PRT

<213> Eremothecium gossypii <400> 51

Met Thr Be Glu Glu Wing Wing Ile Lys Be Read Leu Asp Thr Asp Met1 5 10 15

Tyr Lys Read Thr Met His Wing Val Wing Tyr Val Asn Phe Pro Asp Thr

20 25 30

Glu Val Val Tyr Lys Tyr Thr Asn Arg Be Wing Gly Leu Be Phe Asn

35 40 45

Lys Wing Wing Ile Val Trp Leu Glu Asp Gln Val Ser Lys Wing Leu Thr

50 55 60

Leu Arg Phe Thr Wing Glu Glu Val Wing Tyr Leu Lys Glu Thr Leu Pro65 70 75 80

Phe Leu Pro Wing Gln Tyr Ile Asp Tyr Ile Ser Ser Pro Glu Cys Arg85 90 95

Met Asp Pro Wing Thr Gln Val Gln Leu Read His Glu Gln Arg Glu Gly

100 105 110

Glu Glu Arg Tyr Asp Leu Asn Ile Thr Val Thr Gly Leu Trp Lys Asp

115 120 125

Thr Ile Leu Tyr Glu Ile His Met Leu Wing Leu Ile Ser Glu Wing Tyr

130 135 140

Phe Lys Phe Val Asp Thr Asp Trp Val Read Asp Gly Gln Val Glu Gln145 150 155 160

Wing Tyr Arg Lys Thr Arg Leu Leu Leu Asp Asn Gly Leu Val Phe Ser

165 170 175

Glu Phe Gly Thr Arg Arg Arg Arg Be Ser Leu Val Le Gln Asp Leu Val

180 185 190

Read Gln Gly Ile Glu Wing Val Wing Wing Asp Ser Gly Thr Gly Asp Thr

195 200 205

Gln Phe Ile Gly Thr Be Asn Val Tyr Phe Alys Lys Lys Tyr Gly Val

210 215 220

Lys Pro Val Gly Thr Val Wing His Glu Trp Tyr Met Gly Ile Wing Ala225 230 235 240

Leu Thr Asp Asp Tyr Arg Asn Wing Asn Wing Lys Asn Wing Met Asp Phe Trp

245 250 255

Leu Asn Thr Phe Gly Be Glu Gln Wing Gly Leu Val Leu Thr Asp Thr

260 265 270

Phe Gly Thr Asp Thr Phe Lsu Piro Mst PhG Arcf Pjto Pjto Tyx Ser Asp

275 280 285

Val Tyr Asp Gly Val Arg Gln Asp Ser Gly Asp Pro Wing Val Phe Thr

290 295 300

Glu Lys'Val Wing His His Tyr Leu Asn Val Leu His Tyr Pro Arg Phe305 310 315 320

Ser Lys Val Ile Cys Tyr Ser Asp Ser Leu Asn Pro Glu Lys Ala Leu

325 330 335

Gln Tyr Wing Glu Val Wing Arg Wing His Gly Met Arg Wing Be Phe Gly340 345 350

Ile Gly Thr Asn Phe Thr Asn Ashe Phe Arg Ashe His Ala Pro Asp

355 360 365

Wing Lys Ser Glu Pro Read Asn Ile Val Phe Lys Read Leu Thr Val Asn

370 375 380

Gly Arg Pro Wing Ile Lys Ile Be Asp Asp Read Gly Lys Thr Met Gly385 390 395 400

Asp Pro Glu Lys Val Glu Glu Val Lys Arg Glu Leu Gly Tyr Ile Glu

405 410 415

Arg Thr Trp Glu Gly Ser Asp Glu Wing His Arg Trp Lys Asp420 425 430

<210> 52 <211> 422 <212> PRT

<213> Debaromyces hansenii <400> 52

Met Thr Thr Asp Ile Pro Ile Ile Thr Be Ile Leu Asp Thr Asp Leu15 10 15

Tyr Lys Read Thr Met His Wing Val Wing Val Val His Phe Pro Asn Ile

20 25 30

Pro Val Val Tyr Arg Tyr Asn Arg Thr Pro Be Met Val Leu Asn

35 40 45

Lys Glu Wing Ile Asp Trp Read Lys Tyr Gln Ile Be Lys Read Le Ser Asp

50 55 60

Leu Arg Val Be Ser Glu Glu Leu Glu Tyr Leu Arg Lys Wing Leu Pro65 70 75 80

Gln Met Pro Glu Val Tyr Leu Lys Tyr Leu Glu Thr Phe

85 90 95

Pro Asp Lys Gln Ile Lys Tyr Phe Asn Asp Glu Being Asn Phe Glu

100 105 110

Phe Glu Ile Glu Met Lys Gly Lys Trp Asp Glu Thr Met Leu Tyr Glu115 120 125Ile Pro Leu Read Wing Leu Ile Ser Glu Wing Tyr

130 135 140

Thr Asp Trp Asn Tyr Asp Gly Gln Wing Glu Arg Wing Glu Asp Lys Cys145 150 155 160

Gln Wing Read Phe Lys Asn Glu Cys Thr Phe Be Glu Phe Gly Thr Arg

165 170 175

Arg Arg Arg Be Phe Lys Thr Gln Asp Leu Val Val Lys Asn Leu Cys

180 185 190

Asp Phe Ala Gly Lys Asn Pro Asp Lys Lys His Phe Read Gly Thr Ser

195 200 205

Asn Val Leu Leu Wing Lys Tys Asyr Thr Thr Pro Ile Gly Thr Val

210 215 220

Wing His Glu Trp Phe Met Gly Ile Wing Ser Ile Thr Gln Asp Tyr Thr225 230 235 240

Asn Wing Asn Lys Read Ala Met Asp Tyr Trp Read Asp Thr Phe Gly Pro

245 250 255

Glu His Wing Gly Leu Wing Leu Thr Asp Thr Phe Gly Thr Asp Wing Tyr

260 265 270

Read Lys Val Phe Thr Lys Pro Tyr Thr Asp Tyr Tyr Thr Gly Val Arg

275 280 285

Gln Asp Ser Gly Asp Pro Glu Leu Phe Wing Glu Lys Leu Wing Asp His

290 295 300

Tyr Lys Lys Gln Gly Tyr Pro Asp Phe Ser Lys Ile Ile Cys Phe Ser305 310 315 320

Asp Ser Leu Asn Val Glu Lys Cys Leu Lys Tyr Lys Gln Lys Val Asn

325 330 335

Be Leu Gly Leu Ile Wing Be Phe Gly Ile Gly Thr Phe Phe Thr Asn

340 345 350

Asp Phe Ala Be Val Be Asp Pro Be Thr Lys Be Val Pro Read Asn

355 360 365

Ile Val Ile Lys Leu Lys Glu Wing Asn Gly Asn Pro Ser Ile Lys Ile370 375 380Ser Asp Asn Leu Gly Lys Asn Met Gly Asp Pro

Val Lys Lys Glu Read Asn Tyr Glu Glu His Ser Trp Wing Glu Gly Asp

405 410 415

Glu Glu Lys Arg Trp Asp420

<210> 53 <211> 421 <212> PRT

<213> Candida albicans <400> 53

Met Asn Gly Thr Glu Asp Lys Pro Val Ile Lys Ser Phe Read Asp Thr1 5 10 15

Asp Read Tyr Lys Read Phe Met His Wing Val Wing Asn Lys Gln Phe Pro

20 25 30

Asp Val Pro Val Lys Tyr Arg Tyr Thr

35 40 45

Read Asn Ser Gln Wing Ile Ser Trp Read Lys Lys Gln Ile Glu Tyr Leu

50 55 60

Gly Asp Leu Arg Phe Ser Wing Glu Glu Ile Leu Tyr Leu His Arg Val65 70 75 80

Leu Pro Gln Leu Pro Ser Asp Tyr Leu Glu Tyr Leu Wing Asp Phe Lys

85 90 95

Leu Val Pro Be Ser Gln Ile Lys Tyr Leu Asp Asn Asp Asu Glu Gly Asp

100 105 110

Phe Glu Leu Glu Ile Val Gly Lys Trp Asn Asp Thr Ile Leu Tyr Glu

115 120 125

Ile Pro Leu Leu Wing Leu Val Ser Glu Wing Tyr Phe Lys Phe Val Asp

130 135 140

Thr Asp Trp Asn Tyr Glu <31y Gln Tyr Wing Leu Wing Gln Lys Lys Ala145 150 155 160.

Lys Gln Read Ile Be Asn Glu Cys Asn Phe Be Glu Phe Gly Thr Arg165 170 175

Arg Arg Arg Be Tyr Glu Be Gln Glu Ile Val Ile Lys

180 185 190

Asp Val Gln Ile Asp Thr Gln Be Lys Tyr Ile Wing Gly Thr Be Asn

195 200 205

Val Tyr Phe Ala Met Lys Tyr Asp Leu Pro Pro Ile Gly Thr Val Ala

210 215 220

His Glu Trp Tyr Met Gly Ile Ala Ser Ile Thr Gln Asp Tyr Val His225 230 235 240

Wing Asn Lys Read Wing Wing Met Asp Tyr Trp Ile Asp Thr Phe Gly Wing Lys

245 250 255

Tyr Ala Gly Leu Ala Leu Thr Asp Thr Phe Gly Thr Asp Asn Tyr Leu

260 265 270

Thr Met Phe Val Pro Wing Tyr Val Asn Glu Tyr Ser Gly Val Arg Gln

275 280 285

Asp Ser Gly Asp Pro Glu Read Tyr Wing Glu Lys Ile Wing Arg His Tyr

290 295 300

Glu Lys Met Gly Ile Wing Lys Asn Thr Lys Ile Ile Cys Phe Ser Asp305 310 315 320

Be Leu Asn Val Glu Lys Cys Leu Lys Tyr Lys Asn Thr Wing Asp Lys

325 330 335

Read Gly Read Ile Be Thr Phe Gly Ile Gly Thr Phe Phe Thr Asn Asp

340 345 350

Phe Asn Lys Leu Be Asn Gly Glu Lys Be Gln Pro Met Asn Ile Val

355 360 365

Ile Lys Ile Lys Glu Wing Asn Gly Lys Pro Wing Ile Lys Ile Ser Asp

370 375 380

Asn Ile Gly Lys Asn Met Gly Asp Gln Wing Thr Val Asp Arg Val Lys385 390 395 400

Gln Glu Read Gly Tyr Thr Glu Arg Thr Trp Being Glu Gly Asp Glu Thr

405 410 415

His Arg Trp Ser Lys420

<210> 54 <211> 414

<212> PRT

<213> Yarrowia Iipolytica <400> 54

Met Be Thr Ile Thr Be Read Leu Asp Thr Asp Leu Tyr Lys Leu Thr1 5 10 15

Met Gln Wing Wing Val Leu Gln His Phe Pro Wing Wing Gln Wing Thr Phe

20 25 30

Read Phe Lys Asn Arg Thr Pro Be Lys Gln Read Asn Asp Asp Asp Wing Ile

35 40 45

Glu Trp Leu Lys Ser Glu Ile Wing Wing Leu Gly Glu Leu Arg Phe Thr

50 55 60

Glu Asp Glu Ile Val Phe Leu Gln Lys His Val Gly Phe Leu Pro Ala65 70 75 80

Glu Tyr Phe Glu Tyr Read Lys Thr Cys Read Gln Read Asp Pro Wing Wing Gln

85 90 95

Val Lys Val Thr Val Asn Thr Glu Gly His Leu Glu Ile Glu Val Asn

100 105. 110

Gly Pro Trp Lys Asp Ile Thr Leu Tyr Glu Ile Pro Leu Leu Wing Leu

115 120 125

Val Ser Glu Wing Tyr Phe Lys Phe Val Asp Lys Asp

130 135 140

Gly Gln Ser Glu Read Wing Ward Thr Lys Wing Gln Glu Read Le Ile Wing Gln145 150 155 160

Gly Cys Ala Phe Be Glu Phe Gly Thr Arg Arg Arg Arg Be Read Lys

165 170 175

Thr His Asp Ile Val Ile Wing Gly Ile Leu Glu Gly Leu Lys Ser Ala

180 185 190

Gln Gly Asn Gly Ile Phe Thr Gly Thr Being Asn Val Tyr Leu Wing Lys195 200 205Lys Tyr Asn Leu Lys Pro Ile Gly Thr Val Wing His Glu Trp Met Met

210 215 220

Gly Val Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing Wing

Met Glu Read Trp Ile Gln Thr Val Gly Asp Wing Asn Wing Gly Val Wing

245 250 255

Read Asp Thr Ashe Ply Gly Thr Glu Be Phe Read Asp Ashe Phe Asn Lys

260 265 270

Pro Read Thr Asp Ile Tyr Asn Gly Val Arg Gln Asp Being Gly Asp Pro

275 280 285

Read Glu Tyr Thr Lys Read Leu Gly Asp His Tyr Lys Gln Read Le Gly Tyr

290 295 300

Glu Pro Met Be Lys Val Ile Val Tyr Be Asp Be Read Asp Val Glu305 310 315 320

Lys Cys Gly Lys Tyr Lys Wing Wing Wing Wing Glu Wing Asn Gly Leu Lys Wing

325 330 335

Phe Gly Wing Val Gly Phe Phe Thr Asn Asp Phe Lys Arg Leu Ser

340 345 350

Asp Gly Gln Lys Be Thr Pro Read Asn Ile Val Ile Lys Ile Gln Gln

355 360 365

Leu Asn Gly Gln Be Cys Ile Lys Leu Be Asp Asn Leu Be Lys Asn

370 375 380

Met Gly Asp Pro Glu Thr Val Glu Arg Val Lys Arg Glu Leu Gly Tyr385 390 395 400

Val Glu Lys Gly Asp Val Ile Asp Glu Ser Lys Arg Trp Asn405 410

<210> 55 <211> 490 <212> PRT

<213> Aspergillus fumigatus <400> 55

Met Ser Gln Glu Thr Thr Pro Tyr Pro Glu Gly Ile Phe Ser Leu1 5 10 15

Read Asp Thr Asp Read Tyr Lys Read Thr Met Gln Cys Wing Ile Read Lys

20 25 30

Tyr Phe Pro Asp Val His Val Tyr Gly Phe Asn Arg Thr Pro

35 40 45

Asp Met Lys Leu Thr Arg Gly Wing Tyr Lys Trp Leu Read Glu Gln Met

50 55 60

Asp Arg Leu Wing Asn Val Arg Val Thr Asp Glu Glu Ile Wing Phe Leu65 70 75 80

Lys Lys Gln Cys Pro Tyr Phe Asn His Wing Tyr Leu Arg Tyr Leu Ser

85 90 95

Thr Phe Gln Read Lys Pro Be Glu Ile Asp Ile Lys Phe Arg Pro

100 105 110

Val Gln Asp Be Gly As Asp Asp Asp Read Gly Asp Ile Glu Tyr Met

115 120 125

Val Lys Gly Leu Trp Val Glu Thr Ile Leu Tyr Glu Ile Pro Leu Leu

130 135 140

Wing Read Thr Be Gln Wing Tyr Phe Met Phe Thr Asp Lys Asp Trp Asp145 150 155 160

His Ser Asn Gln Glu Glu Lys Ala Phe Arg Lys Gly Cys Thr Leu Leu

165 170 175

Glu Asn Gly Cys Ile Phe Be Glu As Phe Gly Be Arg Arg Arg Arg Asp

180 185 190

Tyr His Thr Gln Asp Leu Val Met Gln Gly Leu Cys Arg Wing Wing Glu

195 200 205

Gly Gly Lys Arg Gly Gly Trp Lys Gly Val Phe Be Gly Thr Be Asn

210 215 220

Val His Phe Ala Met Tyr Gly Val Thr Pro Ile Gly Thr Val Ala225 230 235 240

His Glu Trp Phe Met Wing Ile Wing Wing Ile Thr Asp Asp Tyr Glu Asn

245 250 255

Wing Asn Glu Leu Wing Wing Leu Arg Tyr Trp Leu Gly Cys Phe Gly Glu Gly260 265 270

Val Leu Gly Ile Wing Leu Thr Asp Thr Phe

275 280 285

Asp Wing Phe Arg Lys Pro Ile Pro His His Be Wing Gly Val Gly

290 295 300

Wing Val Wing Wing Thr Ile Wing Wing Gly Wing Wing Wing Thr Wing Wing Gln305 310 315 320

Pro Gln Thr Glu Wing Glu Thr Lys Pro Val Thr Wing Pro Leu His

325 330 335

Glu Asn Asp Being Gln His Being Pro Lys Thr Tyr Wing Gln Val Tyr Thr

340 345 350

Gly Val Arg Gln Asp Being Gly Asp Pro Thr Tyr Phe Val Lys Met Val

355 360 365

Arg Asp Phe Tyr Asp Asn Glu Gly Ile Lys Glu Lys Lys Thr Val Val

370 375 380

Phe Ser Asp Be Read Asn Ile Glu His Cys Read Glu Tyr Lys Thr Ile385 390 395 400

Glu Glu Wing Gly Phe Wing Pro Ile Phe Wing Gly Val Gly Thr Phe Phe

405 410 415

Thr Asn Asp Phe Thr Asn Lys Be Asp Gly Lys Lys Be Lys Pro Leu

420 425 430

Asn Ile Val Ile Lys Ile Wing Thr Wing Asn Gly Arg Pro Wing Val Lys

435 440 445

Read Ser Asp Asn Met Gly Lys Asn Thr Gly Asp Lys Asn Met Val Gln

450 455 460

Glu Val Lys Lys Arg Read Gly Tyr Ile Glu His Tyr Trp Glu Glu Gly465 470 475 480

Asp Glu Be Asn Arg Trp Wing Lys Gln Gly485 490

<210> 56 <211> 489 <212> PRT <213> Aspergillus oryzae <400> 56

Met Asp Gln Glu Gly Pro Ile Pro Glu Gly Pro Ile Cly Ser Leu1 5 10 15

Leu Asp Thr Asp Leu Tyr Lys Leu Thr Met Gln Cys Wing Val Leu Lys

20 25 30

Tyr Phe Thr Asp Thr His Val Thr Tyr Gly Thr Asn Arg Thr Thr

35 40 45

His Met Lys Read Thr Arg Gly Wing His Lys Trp Read Leu Lys Gln Met

50 55 60

Asp Arg Leu Wing Asn Ile Arg Ile Thr Glu Glu Glu Ile Lys Phe Leu65 70 75 80

Lys Thr Arg Cys Pro Tyr Phe Asn Asp Wing Tyr Leu Asp Phe Leu Thr

85 90 95

Thr Phe Lys Leu Lys Pro Be Glu Gln Ile Glu Ile Lys Phe Thr

100 105 110

Val Asn Asp Thr Gly Be Asp Be Asp Thr Gly Asp Val Glu Tyr Leu

115 120 125

Val Lys Gly Leu Trp Val Asp Thr Ile Leu Tyr Glu Ile Pro Leu Leu

130 135 140

Wing Read Thr Be Glu Wing Tyr Phe Met Phe Be Asp Lys Asp Trp Asp145 150 155 160

Tyr Ser Cys Gln Glu Glu Lys Wing Tyr Arg Lys Gly Cys Thr Leu

165 170 175

Glu Asn Gly Cys Ile Phe Be Glu As Phe Gly Be Arg Arg Arg Arg Asp

180 185 190

Tyr His Thr His Asp Leu Val Met Val Gly Leu Met Lys Wing Glu Wing

195 200 205

Glu Gly Lys Arg Gln Gly Trp Lys Gly Arg Phe Thr Gly Being Ser Asn

210 215 220

Val His Phe Met Lys Wing Tyr Gly Val Asp Pro Val Gly Thr Val Wing Ala225 230 235 240His Glu Trp Tyr Met Thr Ile Wing Ile Thr Asp Tyr Glu Asn

245 250 255

Wing Asn Glu Leu Wing Leu Arg Tyr Trp Leu Gly Cys Phe Gly Lys Gly

260 265 270

Val Leu Gly Ile Wing Leu Thr Asp Thr Phe

275 280 285

Asp Wing Phe Arg Lys Pro Wing Ile Pro Wing Phe Thr Be Wing Gly Wing Gly

290 295 30Ò

Val Wing Be Thr Be Wing Be Gly Pro Wing Thr Thr Wing Asn Glu Be Thr305 310 315 320

Val Gln Ser Glu Wing Glu Thr Lys Pro Wing Ile Thr Pro Wing Read Arg

325 330 335

Asp Gly Gly Wing Arg Thr Be His Glu Thr Tyr Wing Gln Wing Tyr Thr

340 345 350

Gly Val Arg Gln Asp Being Gly Asp Pro Val Tyr Phe Val Lys Met Val

355 360 365

Arg Asp Phe Tyr Asp Arg Glu Gly Ile Thr Asp

370 375 380

Phe Ser Asp Ser Leu Asn Ile Glu His Cys Leu Glu Tyr Lys Val Ile385 390 395 400

Glu Glu Wing Gly Phe Wing Gln Pro Val Phe Gly Val Gly Thr Phe Phe

405 410 415

Thr Asn Asp Phe Thr Asn Lys Be Asn Asp Glu Lys Be Lys Pro Leu

420 425 430

Asn Ile Val Ile Lys Ile Be Thr Wing Asn Gly His Pro Val Lys Wing

435 440 445

Read Asp Asn Met Met Gly Lys Asn Thr Gly Asp Lys Gln Lys Val Gln

450 455 460

Glu Val Lys Lys Lys Read Gly Tyr Val Glu His Glu Trp Glu Glu Gly465 470 475 480

Asp Glu Ser Asn Arg Trp Ser Lys Arg485 <210> 57 <211> 477 <212> PRT

<213> Neurospora crassa <400> 57

Met Asp Phe Asn Be Ser Leu Pro His Pro Wing Gly Val Ile Ser Phe1 5 10 15

Leu Asp Thr Asp Leu Tyr Lys Leu Thr Met Gln Cys Wing Val Leu Lys

20 25 30

Tyr Phe Lys Asp Val Pro Val Thr

35 40 45

Glu Lys Lys Leu Be Arg Glu Wing Phe Val Trp Leu Glu Glu Gln Val

50 55 60

Met Lys Read Gly Asn Ile Be Read Be Pro Glu Glu Read Le Gln Phe Leu65 70 75 80

Lys Thr His Cys Pro Tyr Leu Thr Glu Glu Tyr Leu Asp Tyr Leu Ser

85 90 95

Glu Phe Arg Leu Arg Pro Arg Glu Gln Val Wing Val Ser Phe Arg Pro

100 105 110

Asp Gly Asp Being Asp Read Gly Asp Ile His Tyr Asp Ile Lys Gly Asn

115 120 125

Trp Wing Glu Thr Ile Leu Tyr Glu Ile Pro Leu Leu Wing Leu Thr Ser

130 135 140

Glu Wing Tyr Phe Lys Phe Met Asp Thr Asp Trp Asp Tyr Asp Gly Gln145 150 155 160

Glu Glu Lys Wing Tyr Glu Lys Gly Met Arg Leu Read Leu Glu Wing Gly Cys

165 170 175

Val Phe Be Glu Phe Gly Arg Arg Arg Arg Arg Asp Tyr His Thr Gln

180 185 190

Wing Leu Val Phe Arg Gly Leu Thr Lys Wing Wing Lys Glu Wing Glu Lys

195 200 205

Arg Gly Read Thr Gly Lys Read Be Gly Thr Be Asn Val His Leu Ala210 215 220

Met Arg Phe Asn Ile Pro Pro Val Gly Thr Val Wing His Glu Trp Phe225 230 235 240

Met Gly Asn Ala Wing Ile Leu Gly Asp Tyr Lys Ser Ala Thr Glu Glu

245 250 255

Wing Read Ser Arg Trp Val Gly Cys Tyr Gly Pro Gly Val Leu Gly Ile

260 265 270

Wing Read Thr Asp Thr Phe Gly Thr Pro Glu Phe Leu Arg Wing Phe Ser

275 280 285

Lys Pro Met Be Ala Be Gly Glu Pro Val Pro Gln Pro Arg Asp Arg

290 295 300

Lys Ile Be Thr Wing Asp Phe Wing Ile Be Wing Wing Lys Asp Phe Ile305 310 315 320

Lys Asp Read His Pro Asp Lys Thr Tyr Wing Gln Val Phe Thr Gly Val

325 330 335

Arg Gln Asp Be Gly Asp Pro Lys Glu Phe Val Lys Read Met Arg Lys

340 345 350

Phe Tyr Asp Glu Gln Gly Ile Lys Asp Lys Val Ile Val Phe Ser

355 360 365

Asp Be Read Asp Ile Asp Arg Cys Read Glu Tyr Lys Glu Val Wing Glu

370 375 380

Wing Gly Phe Gln Pro Thr.Phe Gly Val Val Gly Thr Tyr Phe Thr Asn385 390 395 400

Asp Phe Val His Lys Wing Thr Gly Lys Lys Be Thr Pro Read Asn Ile

405 410 415

Val Ile Lys Leu Ser Ser Ala Wing Gly Asn Pro Wing Val Lys Ile Ser

420 425 430

Asp Asn Val Gly Lys Asn Thr Gly Asp Lys Wing Val Thr Glu Lys Val

435 440 445

Lys Arg Glu Read Gly Tyr Val Glu Lys Asp Trp Be Glu Gly Asp Glu

450 455 460

Be Wing Arg Trp Gly His Asp Gly Asp Wing Ala Thr Wing Ala465 470 475

<210> 58 <211> 481 <212> PRT

<213> Chaetomivuri globosum <400> 58

Met Asp Phe Asn Be Ser Ser Ser Tyr Pro Glu Gly Val Ile Ser Phe1 5 10 15

Read Asp Thr Asp Read Tyi "Lys Leu Thi * Met Gln Cys Wing Val Leu Lys

20 25 30

Tyr Tyr Lys Asn Val Pro

35 40 45

Glu Lys Lys Read Be Arg Lys Wing Phe Arg Trp Read Glu Asp Gln Val

50 55 60

Arg Lys Read Gly Asn Ile Be Read Be Wing Glu Glu Read Tyr Tyr Leu65 70 75 80

Lys Glu His Cys Pro Tyr Leu Ser Pro Wing Tyr Leu Glu Phe Leu Ser

85 90 95

Glu Phe Arg Leu Arg Pro Arg Glu Gln Val Val Leu Ser Phe Leu Pro

100 105 110

Thr Gly Glu Asp Thr Gly Glu Wing Be Asp Ile Gly Asp Read Asp Ile

115 120 125

Lys Ile Ser Gly Ile Trp Ser Glu Wing Ile Leu Tyr Glu Ile Pro Leu

130 135 140

Read Be Read Read Be Glu Wing Tyr Phe Lys Phe Met Glu Pro Asp Trp145 150 155 160

Thr Tyr Glu Gly Gln Glu Asp Gln Wing Phe <31u Lys Gly Met Arg Leu

165 170 175

Read Glu Wing Gly Cys Val Phe Be Glu Phe Gly Thr Arg Arg Arg Arg

180 185 190

Asp Tyr His Thr Gln Wing Leu Val Phe Arg Gly Leu Thr Lys Wing Ser195 200 205Lys Glu Wing Glu Lys Arg Gly Leu Being Gly Lys Leu Being Gly Thr Ser

210 215 220

Asn Val His Leu Wing Met Arg Phe Asn Ile Pro Pro Val Gly Thr Val225 230 235 240

Wing His Glu Trp Phe Met Gly Ser Wing Wing Ile Val Gly Asp Tyr Arg

245 250 255

Lys Wing Thr Glu Glu Wing Leu Arg His Trp Val Gly Cys Phe Gly Glu

260 265 270

Gly Val Leu Gly Ile Wing Leu Thr Asp Thr Phe Gly Thr Pro Asp Phe

275 280 285

Leu Thr Wing Phe Ser Lys Pro Ile Glu Tyr

290 295 300

Thr Thr Wing Arg Lys Pro Be Val Wing Asp Be Phe Ile Be Thr Ser305 310 315 320

Pro Be Val Wing Be His Gln Lys Pro Asn Wing Lys Thr Tyr Wing Glu Val

325 330 335

Phe Thr Gly Val Arg Gln Asp Being Gly Asp Pro Lys Thr Phe Val Lys

340 345 350

Ile Ile Gly Lys Phe Tyr Arg Glu Gln Gly Ile Lys Asp

355 360 365

Ile Val Phe Being Asp Being Read Asn Ile Asp Arg Cys Read Glu Tyr Lys

370 375 380

Gln Val Ser Glu Glu Gly Phe Wing Gln Pro Thr Phe Gly Val Gly Thr385 390 395 400

Phe Leu Thr Asn Asp Phe Val Asn Thr Lys Thr Gly Lys Lys Be Thr

405 410 415

Pro Leu Asn Ile Val Ile Lys Le Ser Sera Asp Gly Asn Pro Ala

420 425 430

Ile Lys Ile Be Asp Asn Ile Gly Lys Asn Thr

435 440 445

Val Glu Lys Val Lys Arg Glu Leu Gly Tyr Val Glu Lys Met Trp Glu450 455 460Gly Gly Asp Glu Thr Wing Arg Trp Gly Arg Glu Asp Asp Wing Pro Lys465 470 475 480

Gln

<210> 59 <211> 487 <212> PRT

<213> Aspergillus nidulans <400> 59

Met Gly Glu His Ser Leu Read Asp Gly Val Phe Ser Leu Read Asp1 5 10 15

Thr Asp Leu Tyr Lys Leu Thr Met Gln Cys Ala Ile Leu Lys Tyr Phe

20 25 30

Pro Asp Val Gln Val Thr Tyr Gly Phe Thr Asn Arg Thr

35 40 45

Lys Leu Thr Arg Gly Wing Tyr Lys Trp Met Leu Wing Gln Met Asp Lys

50 55 60

Leu Wing Asn Ile Arg Val Thr Glu Asp Glu Ile Wing Phe Leu Lys Arg65 70 75 80

Arg Cys Pro Tyr Phe Asn Thr Wing Tyr Read Asp Phe Read Thr Asn Phe

85 90 95

Arg Read Lys Pro Be Glu Gln Ile Asp Ile Asn Phe Thr Pro Val Asn

100 105 110

Asp Thr Gly Be Asp Be Asp Phe Gly Asp Ile Asp Tyr Ile Val Lys

115 120 125

Gly Wing Trp Val Asp Thr Ile Leu Tyr Glu Ile Pro Leu Leu Leu Leu

130 135 140

Thr Be Gln Wing Tyr Phe Met Phe Be Asp Lys Asp Trp Asn Tyr Glu145 150 155 160

Cys Gln Glu Gly Lys Wing Tyr Arg Lys Gly Tyr Val Leu Leu Glu Asn

165 170 175

Gly Cys Thr Phe To Be Glu Phe Gly Thr Arg To Arg To Arg To Be Tyr His180 185 190

Thr Gln Asp Leu Val Met Gln Gly Leu Cys Arg Wing Wing Arg Glu Gly

195 200 205

Lys Ala Lys Gly Leu Pro Gly Val Phe Thr Gly Ser As Asn Val His

210 215 220

Phe Ala Met Lys Tyr Asp Val Asp Pro Val Gly Thr Val Wing His Glu225 230 235 240

Trp Tyr Met Thr Ile Asa Wing Ile Thr Asp Asp Tyr Glu Asn Asn Wing

245 250 255

Glu Met Wing Leu Lys Tyr Trp Leu Gly Cys Phe Gly Glu Gly Val Leu

260 265 270

Gly Ile Wing Read Asp Thr Phe Gly Ile Wing Read Ashe Phe Wing Asp

275 280 285

Phe Arg Lys Ile Pro Asp Tyr Thr Be Wing Gly Wing Gly Wing Val

290 295 300

Be Thr Thr Wing Be Gly Pro Be Thr Thr Wing Glu Be Asn Ile Gln305 310 315 320

Be Glu Wing Glu Thr Wing Lys Pro Ile Thr Wing Pro Leu Be Pro Asp

325 330 335

His Pro Pro Val Wing Lys Thr Tyr Wing Gln Val Tyr Wing Gly Val

340 345 350

Arg Gln Asp Being Gly Asp Pro Being Tyr Phe Val Lys Met Wing Arg Asp

355 360 365

Phe Tyr Asp Arg Glu Gly Ile Thr Gly Thr Lys Thr Val Val Phe Ser

370 375 380

Asp Ser Leu Asp Ile Glu His Cys Leu Glu Tyr Lys Val Leu Wing Glu385 390 395 400

Glu Wing Gly Phe Lys Pro Val Phe Gly Val Gly Phe Phe Thr Asn

405 410 415

Asp Phe Ile Asn Thr Thr Thr Asn Glu Lys Be Gln Pro Read Asn Ile

420 425 430

Val Ile Lys Ile Ser Ser Ward Asn Gly Arg Wing Ward Val Lys Leu Ser435 440 445

Asp Asn Met Gly Lys Asn Thr Gly Asp Lys Asp Thr Val

450 455 460

Lys Lys Arg Read Gly Tyr Val Glu His Glu Trp Glu Glu Gly Asp Glu465 470 475 480

Arg Asn Arg Trp Wing Arg Lys485

<210> 60 <211> 459 <212> PRT

<213> Gibberella moniliformis <400> 60

Met Asp Phe Asn Ser Be Ser Pro Ser Phe Pro Glu Gly Val Ile Ser Phe1 5 10 15

Read Asp Thr Asp Read Tyr Lys Read Thr Met Gln Cys Wing Val Phe Lys

20 25 30

Phe Phe Lys Asp Val Pro Val Thr

35 40 45

Asp Lys Lys Leu Be Arg Thr Wing Phe Lys Trp Leu Glu Glu Gln Ile

50 55 60

Arg Lys Read Gly Asn Ile Be Read Be Thr Asp Glu Tyr Leu Phe Leu65 70 75 80

Lys Lys His Cys Asp Tyr Leu Being Asp Asp Tyr Leu Asn Phe Leu Lys

85 90 95

Glu Phe Arg Leu Be Pro Arg Glu Gln Val Val Wing Thr Phe Thr Pro

100 105 110

Val Gly Glu Asp Asn Gly Asp Asp Ser Ile Glu Gly Asp Val Asp Ile

115 120 125

Gln Ile Lys Gly Thr Trp Val Asp Thr Ile Read Tyr Glu Ile Pro Met

130 135 140

Leu Wing Leu Thr Be Glu Wing Tyr Phe Lys Phe Met Asp Thr Asp Trp145 150 155 160Asn Tyr Glu Gly Gln Glu Lys Gln Wing Phe Glu Lys Gly Leu Lys Leu

165 170 175

Read Glu Wing Gly Cys Ile Thr Be Glu Phe Gly Thr Arg Arg Arg Arg

180 185 190

Asp Tyr His Thr Gln Wing Leu Val Phe Arg Gly Leu Val Gln Wing Ser

195 200 205

Lys Glu Wing Glu Lys Lys Gly Phe Pro Gly Lys Read Gly Thr Be

210 215 220

Asn Val His Leu Wing Met Arg Phe Asn Ile Pro Pro Val Gly Thr Val225 230 235 240

Wing His Glu Trp Phe Met Gly Val Wing Wing Ile Ile Gly Asp Tyr Arg

245 250 255

Be Wing Thr Glu Val Wing Read Arg His Trp Val Wing Cys Phe Gly Asn

260 265 270

Lys Leu Gly Ile Wing Leu Thr Asp Thr Phe Gly Thr Gln Glu Phe Leu

275 280 285

Arg Wing Phe Thr Gln Pro Val Gln Thr Ile Glu Gly Gly Phe Pro Wing

290 295 300

Glu Thr Phe Lys Lys Wing Asp Gly Thr Met Lys Thr Tyr Wing Glu Thr305 310 315 320

Phe Wing Gly Ile Arg Gln Asp Be Gly Asp Pro Wing Glu Tyr Thr Lys

325 330 335

Trp Met Lys Glu Phe Tyr Asp Lys Gln Gly Ile Thr Asp Lys Lys Leu

340 345 350

Ile Val Phe Being Asp Being Read Asn Ile Glu Arg Cys Read Glu Tyr Lys

355 360 365

Lys Ile Wing Glu Asp Leu Gly Phe Gln Pro Thr Phe Gly Val Gly Thr

370 375 380

Tyr Leu Thr Asn Asp Phe Val His Leu Thr Thr Gly Lys Lys Ser Val385 390 395 400

Pro Leu Asn Ile Val Ile Lys Ile Be Ser Asa Wing Gly Arg Pro Ala405 410 415Val Lys Ile Be Asp Asn Ile Gly As Lys Asn Thr Gly Asp Lys Glu Thr

420 425 430

Val Glu Lys Val Lys Gln Glu Leu Gly Tyr Val Glu Arg Glu Trp Lys

435 440 445

Glu Gly Asp Glu Thr Be Arg Trp Gly Lys Glu

450 455

<210> 61 <211> 410 <212> PRT

<213> Schizosaccharomyces pombe <400> 61

Met Ser Glu Pro Val Val Val Ser Ile Leu Asp Thr Asp Leu Tyr Lys1 5 10 15

Leu Thr Met Leu Gln Val Wing Leu Glu His Tyr Pro Asp Wing Gln Val

20 25 30

Be Tyr Lys Tyr Thr Asn Arg Be Pro Lys Met Wing Read Asn Gln Glu

35 40 45

Wing Tyr Asn Trp Leu Arg Glu Ile Arg Gly Leu Arg Asn Leu His

50 55 60

Leu Leu Pro Glu Glu Glu Trn Leu Arg Lys Asn Cys Pro Tyr Leu65 70 75 80

Lys Glu Be Phe Tyr Glu Phe Met His Glu Phe Glu Phe Asp Pro Glu

85 90 95

Asn Be Ile Be Read Asn Tyr Asp Be Glu Thr Lys Asp Be Read Ile

100 105 110

Phe Ile His Gly Leu Trp Lys Asn Thr Ile Phe Tyr Glu Ile Pro Leu

115 120 125

Leu Ala Leu Val Be Glu Be Tyr Phe Lys Phe Val Asp

130 135 140

Ser Pro Glu Gly Lys Phe Glu Lys Wing Tyr Glu Lys Gly Lys Arg Leu145 150 155 160

Ile Arg Wing Gly Cys Wing Phe Thr Asp Phe Gly Thr Arg Arg Arg Arg165 170 175

Asp Pro His Thr Gln Glu Ile Val Leu Gln Gly Leu Met Lys Wing Gln

180 185 190

Glu Asp Phe Lys Gly Pro Gly Be Phe Read Gly Thr Be Asn Val Tyr

195 200 205

Phe Ala Wing Lys Tyr Asn Leu Asn Val Ser Gly Thr Val Wing His Glu

210 215 220

Trp Tyr Met Gly Ile Wing Ile Thr Wing Gln Asn Tyr Lys Gln Wing Asn225 230 235 240

Arg Ile Wing Being Read Lys Trp Val Gln Thr Phe Gly Thr Be Read Leu

245 250 255

Ile Wing Read Thr Asp Thr Phe Be Thr Asp Val Phe Read Lys Be Phe

260 265 270

Thr Wing Asn Be Wing Asp Asp Leu Wing Asn Val Phe His Gly Val Arg

275 280 285

Gln Asp Ser Gly Cys Wing Glu Glu Tyr Ile Glu Lys Val Val Lys His

290 295 300

Tyr Lys Ser Ile Gly Val Asp Pro Be Thr Lys Val Ile Val His Ser305 310 315 320

Asp Wing Leu Asn Val Asp Arg Cys Ile Glu Leu Tyr Lys Tyr Cys Glu

325 330 335

Lys Cys Gly Ile Lys Ser Ala Phe Gly Ile Gly Thr Asn

340 345 350

Asp Phe Gln Lys Val Ser Asn Pro Be Glu Val Be Lys Pro Met Asn

355 360 365

Ile Val Ile Lys Leu Phe Ser Wing Glu Gly Thr Lys Wing Val Lys Ile

370 375 380

Ser Asp Asp Ile Met Lys Asn Thr Gly Asp Arg Asp Wing Val Ile Gln385 390 395 400

Wing Lys His Gln Leu Cys Leu Pro Ile Ala405 410

<210> 62 <211> 1971 <212> PRT

<213> Magnaporthe grisea <400> 62

Met Asp Phe Asn Be Asp Be Pro Phe Pro Glu Gly Val Ile Be Phe1 5 10 15

Leu Asp Thr Asp Leu Tyr Lys Leu Thr Met Gln Cys Wing Val Leu Lys

20 25 30

Tyr Tyr Be Asn Thr Be Val Thr Tyr Be As Phe Thr Asn Arg Thr

35 40 45

Glu Lys Arg Leu Be Arg Lys Wing Tyr Gln Trp Leu Gln Asp Gln Ile

50 55 60

Arg Asn Glu Glu Tyr Arg Phe Read Lys Asp Asn Cys Pro Tyr Leu Gly65 70 75 80

Thr Gln Tyr Leu Glu Phe Leu Gln Asn Phe Arg Leu Asn Pro Arg Asp

85 90 95

Gln Val Ile Val Ser Phe Pro Wing Val Gly Lys His Glu Gly Wing Asp

100 105 110

Be Asp Val Gly Asp Ile Asp Ile Gln Ile Be Gly Lys Trp Val Asp

115 120 125

Thr Ile Leu Tyr Glu Ile Pro Ile Leu Wing Leu Thr Be Glu Ala Tyr

130 135 140

Phe Arg Phe Met Asp Thr Asp Trp Asp Tyr Gln Gly Gln Glu Glu

Wing Tyr Glu Lys Gly Lys Arg Ile Leu Glu Wing Gly Cys Ile Leu Ser

165 170 175

Glu Phe Gly Thr Arg Arg Arg Arg Asp Tyr His Thr Gln Wing Leu Val

180 185 190

Phe Arg Gly Leu Val Lys Wing Ser Lys Glu Wing Gln Lys Arg Gly Leu

195 200 205

Ser Gly Lys Leu Ser Gly Thr Be Asn Val His Leu Wing Met Arg Phe210 215 220Asn Met Pro Wing Val Gly Thr Val Wing His Glu Trp Phe Met Gly Ile225 230 235 240

Wing Wing Ile Ile Gly Asp Tyr Arg Be Wing Be Glu Glu Wing Leu Wing

245 250 255

Arg Trp Val Gly Ala Phe Gly Pro Gly Val Leu Ala Ile Ala Leu Thr

260 265 270

Asp Thr Phe Gly Thr Pro Glu Phe Read Lys Be Phe Be Arg Pro Val

275 280 285

Arg Trp Read Glu Glu Wing His Ser Wing Wing Wing Gly Wing Asn Glu Ser Lys

290 295 300

Thr Tyr Wing Gln Val Phe Wing Gly Val Arg Gln Asp Ser Gly Asp Pro305 310 315 320

Thr Thr Phe Val Lys Arg Met Arg Glu Phe Tyr Asp Gln Gln Arg Ile

325 330 335

Lys Glu Lys Lys Thr Ile Val Phe Be Asp Be Read Asp Ile Asp Arg

340 345 350

Cys Leu Val Tyr Lys Gln Val Ser Glu Wing Gly Phe Wing Gln Pro Thr

355 360 365

Phe Gly Val Gly Thr Phe Leu Thr Asn Asp Phe Val Gly Lys Be Thr

370 375 380

Gly Lys Lys Be Thr Pro Read Asn Ile Val

Asp Gly Lys Pro Wing Val Lys Read Ser Asp Asn Ile Gly Lys Asn Thr

405 410 415

Gly Asp Wing Asn Thr Val Glu Lys Val Lys Arg Glu Ile Gly Tyr Glu

420 425 430

Glu Lys Asp Trp Glu Gly Gly Asp Glu Thr Be Arg Leu Asn Gly Asn

435 440 445

Arg Tyr Asp Arg Gly Wing His Wing Read Met Asp Read Be Asp Pro Wing

450 455 460

Wing Thr Be Arg Be Arg Be Val Val Tyr Phe Phe Lys Be Gly Thr465 470 475 480Thr Read Be Glu Ile Pro Glu Be Pro Gly Arg Be Gly Pro Met

485 490 495

Asn Be Gln Arg Be His Gly Gly Gly Ala Phe Asp Arg Glu Arg Asp

500 505 510

Pro Arg Glu Arg Asp Read Asp Glu His Arg His Arg Pro Read Le Gl Thr

515 520 525

Glu Glu Wing Asn Arg Wing Asp Arg Glu Arg Asp Ile Asp Arg Glu Arg

530 535 540

Glu Arg Glu His Wing Asp Arg Gln Gln Pro Arg Glu Wing Tyr Pro Pro545 550 555 560

Gly Pro Wing Read His Be Thr Gly Pro Wing Ile Pro Ile His Gln Pro

565 570 575

Val Wing Be Arg Ile Wing Gly Val Ile His Be Pro Gly Gly Leu Leu

580 585 590

Gly Ala His Asn Gly Ala Pro Gly Gly Leu Pro Leu Gly Ala Pro Thr

595 600 605

Thr Gln Read Wing Gly Phe Gly Gly Pro Ile His Ser Asp Pro Wing Arg

610 615 620

Gln Met Pro Pro Gln Pro Pro Asn Gln Asn Be Thr Gly Gly Gln Gln625 630 635 640

His Gln Met Phe Pro Wing Ile Pro Gln Pro Gln Wing Gly Gly Pro Asn Gly

645 650 655

Be Gly Gly Pro Gly Gly Be Gly Pro Gly Be Val Phe Gly Gly Pro Leu

660 665 670

Gln Thr Glu Asn Gly Arg Wing Pro Val Gln Gln Wing Pro Pro Wing

675 680 685

Val Val Pro Pro Wing Gln Thr Be Sera Wing Gly Leu Be Gln Gln

690 695 700

Gly Ser Gln Pro Ile Leu Asn Asp Wing Leu Ser Tyr Leu Asp Gln Val705 710 715 720

Lys Wing Gln Phe His Glu Gln Pro Asp Val Tyr Asn Arg Phe Leu Asp725 730 735Ile Met Lys Asp Phe Lys Being Gln Thr Ile Asp Thr Pro Gly Val Ile

740 745 750

Asn Arg Val Ser Asp Leu Phe Ala Gly His Pro Asn Leu Ile Gln Gly

755 760 765

Phe Asn Thr Phe Pro Le Pro Gly Tyr Arg Ile Glu Cys Gly Leu Asp

770 775 780

Asn Asn Pro Asn Ser Ile Arg Val Thr Thr Pro Gly Ser Thr Val785 790 795 800

His Ser Ile Gly Wing Gly Arg Gly Wing Read Pro Pro Val Asp Gly Ser

805 810 815

Wing Gly Pro Pro Gly Gln Asn Wing Gln Tyr Leu Gly Pro Asn Ser Arg820 825 830

835 840 845

Gln Phe Wing Ser Pro Wing Gln Pro Wing Gly Pro Wing Gly Pro Phe Gly Wing

850 855 860

Val Gly Pro Wing Gly Wing Gln Phe Asp Gly His Ser Pro Wing His Gln865 870 875 880

Gln Arg Val Val Being Gly Gln Gln Gln Pro Gly Wing Gly Being Gly

885 890 895

Read Val Val Gly Pro Wing Gly Be Met Pro Wing Gly Pro Met Wing Arg Asn

900 905 910

Val Gln Pro Thr Thr Gly Wing Gln Pro Wing Be Read Asn Gly Ser

915 920 925

Being Gln Gln Gly Gly Gln Arg Gly Pro Val Glu Phe Asn His Wing

930 935 940

Ile Ser Tyr Val Asn Lys Ile Lys Asn Arg Phe Gln Asp Lys Pro Glu945 950 955 960

Ile Tyr Lys Gln Phe Leu Glu Ile Leu Gln Thr Tyr Gln Arg Glu Gln

965 970 975

Lys Pro Ile Gln Glu Val Tyr Ala Gln Val Thr Thr Leu Phe His Thr980 985 990Ala Pro Asp Leu Leu Glu Asp Phe Le Lys Gln Phe Leu Pro Glu Ser Wing

995 1000 1005

Gln Wing Thr Arg Thr Phe Gly Gln Arg Pro Glu Wing Asp Gly Gly

1010 1015 1020

Leu Leu Asn His Ile Asn Gln Met Pro Gln Wing Thr Asn Pro Wing

1025 1030 1035

Glu Arg Gly Pro Lys Met Pro Pro Val Gly Asn Phe Wing Val Pro

1040 1045 1050

Val Be Wing Be Lys Asp Asn Lys Lys Arg Pro Arg Ile Glu Lys

1055 1060 1065

Pro Pro Wing Ward Thr Pro Gln Wing Gln Gln Ser Pro Wing Val Gly

1070 1075 1080

Be Wing Be Ile Asn Be Wing Asn Lys Arg Thr Lys Thr Thr

1085 1090 1095

Lys Pro Be Gly Asp Gly Pro Be Ile Glu Pro Be Read Thr Pro

1100 1105 1110

Ile Val Pro Glu Pro Met Be Pro Be Wing Be Thr Be

1115 1120 1125

Gln Glu Lys Wing Leu Asn Tyr Leu Glu Arg Ile Lys Lys His Ile

1130 1135 1140

Gly Asn Arg Thr Thr Read His Glu Phe Phe Lys Leu Ile Asn Leu

1145 1150 1155

Tyr Thr Gln Gly Leu Ile Asp Lys Asn Val Leu Val Gln Lys Wing

1160 1165 1170

Gln Met Ile Ile Gly Wing Asn Val Glu Read Met Thr Trp Phe Lys

1175 1180 1185

Asn Phe Val Arg Tyr Thr Gly Asp Asp Glu Read Val Glu Asn Lys

1190 1195 1200

Pro Glu Pro Pro Thr Gly Arg Val Be Read Asn Cys Arg Gly

1205 1210 1215

Tyr Gly Pro Be Tyr Arg Leu Leu Arg Lys Arg Glu Arg Leu Lys1220 1225 1230Pro Cys Ser Gly Arg Asp Glu Leu Cys Asn Ser Val Leu Asn Asp

1235 1240 1245

Glu Trp Wing Be His Pro Thr Trp Wing Be Glu Asp Be Gly Phe

1250 1255 1260

Val Wing His Arg Lys Asn Wing Phe Glu Glu Gly Leu His Arg Ile

1265 1270 1275

Glu Glu Glu Arg His Asp Tyr Asp Phe Phe Ile Glu Wing Asn Gln

1280 1285 1290

Lys Cys Ile Gln Read Leu Glu Pro Ile Wing Gln Gln Met Leu Thr

1295 1300 1305

Read Asn Pro Wing Asp Arg Gln Asn Phe Arg Met Pro Be Gly Leu

1310 1315 1320

Gly Gly Gln Being Thr Be Ile Tyr Lys Arg Val Read Lys Lys Val

1325 1330 1335

Tyr Gly Asp Wing Lys Gly Wing Glu Val Val Asn Asp Met Phe Gln

1340 1345 1350

His Pro Phe Thr Val Val Pro Ile Val Met Wing Arg Read Le Lys Gln

1355 1360 1365

Lys Asp Glu Glu Trp Arg Phe Ser Gln Arg Glu Trp Glu Lys Val

1370 1375 1380

Trp Gln Be Gln Thr Lys Ala Met His Read Lys Be Read Asp His

1385 1390 1395

Gln Gly Ile Gln Val Lys Gly Asn Asp Lys Arg Thr Read Ala

1400 1405 1410

Lys His Leu Val Asp Leu Ile Lys Thr Lys His Glu Glu Gln Lys

1415 1420 1425

Arg Gln Arg Val Asn Lys Gly Lys Pro Wing Arg Val Gln His Leu

1430 1435 1440

Trp Ser Phe Thr Asp Gln Gly Val Leu Leu Asp Leu Leu Arg Phe

1445 1450 1455

Met Val Leu Tyr Ala Met Asn Be Gly Gln His Gly Be Ser Glu1460 1465 1470Lys Asp Arg Ile Read Glu Phe Phe Glu Asn Be Val Pro Gln Phe

1475 1480 1485

Phe Gly Ile Leu Pro Glu Gln Val Arg Gln His Leu Gly Asp Ile

1490 1495 1500

Asp Arg Asp Wing Gly Glu Glu Glu Wing Asp Asp Asn Thr Pro Wing

1505 1510 1515

Glu Read Thr Asn Gly Arg Be Arg Arg Asn Gly Lys Lys Be Asp

1520 1525 1530

Leu Leu Arg Gly Leu Leu Asp Pro Gly Arg Asn Gly Be Lys Thr

1535 1540 1545

Arg Be Gln Pro Glu Glu Be Asn Thr Be Wing Be Lys Glu Thr

1550 1555 1560

Thr Pro Asp Gln Gly Ser Thr Asn Glu Glu Glu Met Wing Asp Wing

1565 1570 1575

Pro Asp Asp Gly Ser Wing Thr 'Wing Glu Asn Wing Asn Asn Glu Arg

1580 1585 1590

Trp Leu Gln Asn Pro Pro Lys Wing Thr Val Leu Ser Gly Tyr Asn

1595 1600 1605

Pro Read Be Asp Gly Asp Be Glu Read Lys Wing Asp Gly Read Phe

1610 1615 1620

Pro Arg Pro Trp Tyr Asn Phe Phe Cys Asn Gln Thr Ile Phe Val

1625 1630 1635

Phe Phe Ser Val Phe Gln Thr Read Tyr Lys Arg Read Le Lys Thr Val

1640 1645 1650

Lys Glu Be Arg Glu Be Val Leu Glu Glu Ile Arg Arg Glu Arg

1655 1660 1665

Wing Asp Lys Pro Wing Lys Gln Leu Gly Leu Val His Asp Glu Met

1670 1675 1680

Asn Tyr Phe Asp Gly Asp Pro Glu Thr

1685 1690 1695

Glu Leu Ile Glu Asp Phe Ile Thr Gly Glu Ile Asp Glu Ser Arg1700 1705 1710Cys Gln Asp Val Leu Arg His Tyr Tyr Leu Gln Cys Gly Trp Thr

1715 1720 1725

Read Tyr Thr Ile Gln Asp Read Leu Lys Thr Read Cys Arg Gln Wing

1730 1735 1740

Leu Val Cys Asn Asn Be Asp Asn Lys Glu Lys Thr Pro Asp Leu

1745 1750 1755

Val Gln Gln Phe Leu Thr Wing Lys Ser Gln Glu Glu Thr Ser Tyr

1760 1765 1770

Gln Val Glu Ile Be Wing Arg Lys Phe Wing Glu Lys Cys Ile Lys

1775 1780 1785

Asp Gly Glu Met Phe Met Ile Thr Trp Wing Pro Be Ser Asn Glu

1790 1795 1800

Wing Thr Val Arg Trp Leu Pro Lys Glu Asp Thr Thr Phe His Wing

1805 1810 1815

Glu Glu Met Glu Pro Thr Asp Arg Trp Lys Tyr Tyr Ile Wing Ser

1820 1825 1830

Tyr Met Arg Val Asp Pro Thr Glu Gly Val Pro Lys Leu Arg Leu

1835 1840 1845

Gln Lys Thr Val Leu Wing Arg Asn Leu Pro Be Ser Asp Ser Asp

1850 1855 1860

Be Asp Asp Gly Wing Val Pro Lys Pro Read Be Tyr Be Glu Gly

1865 1870 1875

Gly Leu Arg Le Ile Cys Val Asn Be Ser Lys Ile Val Tyr Glu

1880 1885 1890

Lys Glu Thr Be Glu Tyr Thr Ile Tyr Asn Met Thr Be Read

1895 1900 1905

Glu Glu Arg Arg Wing Phe Asp Arg Asp Arg Arg Wing Gln Leu Phe

1910 1915 1920

Arg Glu Lys Leu Ile Met Asn Asn Wing Trp Met Lys Asp Leu Ser

1925 1930 1935

Gln Val Glu Val Gln Gly Met Asn Glu Asp Phe Gln Arg Trp Ala1940 1945 1950Lys Asp Gly Ile Val Pro Gly Thr Be Ser Thr Gly Gly Wing Gly

1955 1960 1965

Be Be Wing

1970 <210> 63 <211> 453 <212> PRT

<213> Cryptococcus neoformans <400> 63

Met Pro Asp Lys Read His Leu Pro Ile Asp Asp Val Gln Ile Pro Phe15 10 15

Ser Ile Read Asp Thr Asp Read Tyr Lys Read Thr Met Gln Asn Val Wing

20 25 30

Read His His Phe Ser Asp Wing His Val Val Ile Lys Phe Thr Asn Arg

35'40 45

Be Pro Gln Met Leu Phe Be Pro Lys Glu Cys Phe Asp Trp Val Gln Gln

50 55 60

Arg Val Asn Asp Read Lys Read Lys Read Lys Read Thr Be Glu Glu Arg Lys65 70 75 80

Glu Read To Be Lys Wing Cys Pro Tyr Phe To Be Glu To Be Tyr Read Asp Tyr

85 90 95

Read Asn Met Gln Read Asp Pro Val Lys Gln Val Lys Read Thr Phe

100 105 110

Ile Pro Gln Gly Being Asn Glu Lys Gly Glu Lys Met Gly Glu Ile Gly

115 120 125

Cys Val Ile Glu Gly Pro Trys Lys Asp Thr Met Leu Tyr Glu Val Pro

130 135 140

Val Met Wing Ile Read Be Glu Gly Tyr Phe Lys Phe Val Asp Thr Asp145 150 155 160

Trp Asp Tyr Asp Gly Gln Phe Glu Leu Wing Lys Lys Lys Wing Leu Asp

165 170 175

Read Leu Asn Pro Pro Wing Pro Thr Thr Be Read Be Phe Be Glu Phe180 185 190

Gly Thr Arg Arg Arg Arg Be Phe Lys Wing Gln Asp Ile Ile Met Arg

195 200 205

Gly Leu Ile Wing Gly His Glu Glu Tyr Lys Ser Lys Gly Gly Ser Gln

210 215 220

Gly Ile Read Be Gly Thr Be Asn Val Tyr Read Ala Read Le Lys Tyr Gly225 230 235 240

Leu Asn Pro Val Gly Thr Ile Wing His Glu Trp Ile Met Val Gly Wing

245 250 255

Wing Thr Tyr Gly Tyr Arg Gly Wing Asn Gly Arg Wing Met Asp Met Trp

260 265 270

Glu Glu Val Tyr Pro Gly Thr Lys Phe Ser Be Pro Leu Thr Met

275 280 285

Leu Thr Asp Thr Tyr Thr Wing Ile Phe Phe Lys Asp Phe Ile Ser

290 295 300

Asp Pro Wing Arg Wing Wing Leu Arg Trp Wing Val Leu Arg Gln Asp Ser Gly305 310 315 320

Asp Wing Phe Lys Phe Val Glu Asp Wing Lys Glu Wing Trp Arg Thr Ile

325 330 335

Glu Asp Lys Gly Wing Ile Lys Arg Asp Val Gly Pro Asn Gly Glu

340 345 350

Glu Val Lys Wing Gly Lys Lys Val Ile Phe Ser Asp Gly Leu Asp Val

355 360 365

Glu Lys Wing Ile Lys Read Gln Gln Gly Cys Asp Lys Wing Gly Met Wing

370 375 380

Ala Ser Phe Gly Ile Gly Thr Asp Read Thr Asn Asp Phe Arg Lys Ala385 390 395 400

Be Asp Pro Be Gln Lys Be Lys Wing Leu Asn Met Val Ile Lys Leu

405 410 415

Asn Lys Ile Asn Gly Lys Asp Cys Ile Lys Read Being Asp Asp Lys Gly

420 425 430

Lys His Thr Gly Being Leu Glu Glu Val Arg Lys Wing Gln Gln Glu Leu435 440 445

Gly Ile Asp Lys Asn

450 <210> 64 <211> 718 <212> PRT

<213> Cryptococcus neoformans <400> 64

Met Glu Glu Pro Be Pro Be Pro Arg Lys Pro Be Pro Be Pro Pro 5 5 15 15

Be Ser Ser Ser Ser Ser Ala Gly Ser Asu Leu Asp Leu Pro Ser Leu Gln

20 25 30

Pro Read Be His Asp His Pro Read Leu Thr Wing Pro Thr Phe Asp Pro

35 40 45

Asp Wing Phe Leu Leu Be Arg Ile His Ile Pro Leu Glu Glu Leu Arg

50 55 60

Gly Glu Leu Arg Glu Tyr Leu Gly Glu Leu Arg Glu Glu Leu Val Lys65 70 75 80

Leu Ile Asn Glu Asp Tyr Glu Glu Phe Leu Being Leu Gly Ile Gly Leu

85 90 95

Arg Gly Glu Glu Glu Arg Read Le Lys Arg Read Le Glu Gly Pro Read Le Gln Gly

100 105 110

Val Arg Lys Glu Ile Val Ser Val Arg Asp Val Leu Wing Glu His Gln

115 120 125

Wing Lys Leu Gln Glu Lys Leu Asp Glu Arg Wing Wing Leu Arg Glu Glu

130 135 140

Lys Wing Read Leu Asp Read Leu Gln Arg Leu Phe Asp Thr Leu Wing Lys145 150 155 160

Glu Wing Alu Leu Read Asp Leu Pro His Thr Asp Glu Leu Glu Thr Ser

165 170 175

Lys Leu Val Thr Arg Val Wing Gly Glu Tyr Ser Gln Ile Val Tyr Leu180 185Met Asn Lys Leu Arg Thr Glu Wing Gl Cys Ile Val Asn Val Val Glu

195 200 205

Glu Arg Ile Lys Asn Ile Lys To Be Arg Read To Be Lys Asp Read To Be Thr

210 215 220

Val Leu Leu Be Glu Leu Glu Asn Leu Asn Wing Thr Thr Gly Leu Lys Gln225 230 235 240

Cys Leu Lys Thr Tyr Glu Leu Ile Glu Gly Trp Glu Glu Wing Glu Glu

245 250 255

Val Val Arg Lys Val Phe Arg Glu Tyr Cys Arg Asn Thr Ile Ser

260 265 270

Be Wing Read Be Wing Pro Thr Be Wing Pro Thr Wing Gln Thr Wing His

275 280 285

Gln Leu Arg Asn Pro Leu Asp Val Pro Arg Leu Pro Wing Be Tyr Asn

290 295 300

Thr Pro Leu Wing Leu Leu Phe Asn Arg Val Leu Wing Gln Val Wing Ser305 310 315 320

Tyr Gln Pro Read Leu Asp Wing Be Lys Glu Val Be Glu Lys Phe Asp

325 330 335

Phe Phe Ala Arg Val Phe Trp Pro Glu Ile Gly Asp Thr Ile Ile Glu

340 345 350

Arg Leu Gly Ser Val Ile Phe Ala Wing Gly Arg Pro Asp Asp Leu His

355 360 365

Lys Tyr Tyr Thr Thr Be His Lys Phe Read Asp Read Leu Glu Thr Ile

370 375 380

Pro Wing To Be His Wing Asn Val Leu Wing Met Arg To Be Pro To Be Tyr385 390 395 400

Thr Wing Phe Glu Arg Arg Thrp Gln Leu Pro Val Tyr Phe Gln Leu Arg

405 410 415

Trp Lys Glu Ile Val Ser Be Leu Glu Gln Be Leu Wing Gly Gln Pro

420 425 430

Be Tyr Thr Be Thr Be Asp His Lys Gly Be Lys Trp Val Leu Val435 440 445Gln Be Gly Wing Val Trp Lys Wing Leu Glu Be Cys Trp Lys Glu Asp

450 455 460

Val Tyr Ile Be Glu Read Wing Pro Arg Phe Trp Arg Read Le Be Read Gln465 470 475 480

Ile Be Ser Arg Tyr Gly Thr Tyr Read Lys Ser Thr Val Asp Be Tyr

485 490 495

Val Ile Thr Glu Glu Asp Asn Be Gln Glu Asp Wing Ward Leu Arg Phe

500 505 510

Wing Ser Wing Wing Val Val Asp Leu Glu Asn Leu Wing Wing Lys Val Lys

515 520 525

Asp Leu Asp Val Val Lys Glu Leu Asn Leu Gly Glu His Leu Thr Leu

530 535 540

Pro Thr Thr Gln Tyr Thr Be Lys Ile Read Ile Read Ile Read Arg Arg545 550 555 560

Cys Thr Asp Pro Read Lys Read Ile Arg Be Ile Wing Be Gln Phe Arg

565 570 575

Be Be Pro Thr Be Be Pro Be Be Pro Arg Be Gln Be Be Tyr

580 585 590

Phe Val Pro Be Val Phe Lys Pro Read His Be Read Le Be Ser Gln

595 600 605

Pro Gln Read Lys Glu Arg Tyr Gln Gln Asp Phe Be Arg Gln Ile Wing

610 615 620

Asp Wing Val Phe Val Asn Tyr Wing To Be Thr Read Wing To Be Val Lys Lys625 630 635 640

Thr Glu Asp Read Leu Arg Lys His Arg Lys Be Lys Lys Be Gly Ile

645 650 655

Thr Be Phe Phe Gly Gly Gly Gly His Asp Gly Gly Gly Gly Glu Lys

660 665 670

Glu Glu Glu Arg Phe Thr Asn Gln Met Lys Val Asp Ile Asp Wing Leu

675 680 685

Lys Glu Asp Wing Lys Gly Leu Gly Val Asp Pro Glu Be Met Asn Ser690 695 700Trp Asn Glu Leu Leu Wing Val Val Asn Lys Pro Asp Glu Ala705 710 715

<210> 65 <211> 612 <212> PRT

<213> Ustilago maydis <400> 65

Met Cys Wing Pro Wing Be Thr Be Wing Pro Val Wing Lys His Wing Ser1 5 10 15

Asp Wing Be Pro Ile Arg Be Ile Read Asp Thr Asp Read Tyr Lys Leu

20 25 30

Thr Met Gln Gln Wing Val Leu Arg His Tyr Pro His Thr Arg Val Wing

35 40 45

Tyr Lys Phe Thr Asn Arg Be Wing Ward Thr Met Lys Phe Thr Arg

50 55 60

Ala Met Asp Arg Ile Arg Asn His Ile Asp Asn Leu Val His Leu Ser65 70 75 80

Leu Ser Gla Glu Arg Wing Trp Leu Ser Glu Arg Cys Pro Tyr Leu

85 90 95

Gly Lys Asp Tyr Read Asp Tyr Read Glu Wing Phe Arg Phe Gln Pro Lys

100 105 110

Gln Gln Val Gln Read Arg Phe Read Pro Thr Gly Glu Asp Gly Trp Gly

115 120 125

His Leu Asp Leu Asn Val Ser Gly Val Trp Ser Asp Val Ile Phe Tyr

130 135 140

Glu Val Pro Leu Met Wing Ile Val Ser Glu Val Tyr Phe Ser Thr Ile145 150 155 160

Asp Thr Asp Trp Being Read Gln Asp Gln Tyr Gln Gln Wing Phe Asp Lys

165 170 175

Wing Cys Arg Read Thr Be Asn Gly Ile Arg Tyr Be Glu Phe Gly Thr

180 185 190

Arg Arg Arg Arg Ser. Tyr Gln Thr His Arg Ile Val Leu Gln Gly Leu195 200 205

Met Wing Gly Asp Read Be Wing Be Ser Gly Cys Be Wing Gly Lys Leu

210 215 220

Read Gly Thr Be Asn Val His Phe Wing Gln Gln Phe Asp Read Val Pro225 230 235 240

Ile Gly Thr Val Wing His Glu Trp Thr Met Wing Ile Wing Wing Leu Gln

245 250 255

Gly Tyr Wing His Ser Asn Leu Lys Wing Leu Gln Leu Trp Asp Wing Val

260 265 270

Tyr Ser Wing Pro Asp Phe Val Wing Asn Ser Wing His Thr Asp Leu Thr

275 280 285

Ile Wing Leu Thr Asp Thr Phe Ser Thr Asn Val Phe Trp Asn Asp Leu

290 295 300

Leu Asp Asn Pro Be Gly Ile Glu Ile Wing Arg Arg Trp Arg Gly Leu305 310 315 320

Arg Gln Asp Be Gly Asp Be Lys Wing Phe Wing Gln Lys Wing Read Asp

325 330 335

Tyr Wing Arg Be Ile Gly Val Asp Pro Lys Be Lys Val Val Ile Tyr

340 345 350

Ser Asp Gly Leu Asp Val Asp Arg Cys Leu Glu Leu Wing Wing Tyr Ser

355 360 365

Asn His Ile Gly Ile Gly Ala Wing Phe Gly Ile Gly Thr Be Phe Thr

370 375 380

Asn Asp Phe Ile Gln Read Be Thr Wing Gln Lys Be Lys Pro Read Asn385 390 395 400

Ile Val Ile Lys Leu Asp Ser Val Glu His Arg Arg Val Val Lys Ile

405 410 415

Ser Asp Asp Leu Thr Thr Lys Asn Thr Gly Asp Pro Thr Glu Val Leu Wing

420 425 430

Val Lys Arg Arg Phe Gly Ile Pro Thr Be Ser Ala Thr Pro Ala

435 440 445

Asp Wing Ser Val Ile Asn His Leu Asp Pro Gly Wing Pro Pro Asn Pro450 455 460

Ser Be His Wing His Leu Gly Thr Asp Asp Wing Thr Met Thr Gln Val465 470 475 480

Ser Pro Read Asp Gln Arg Asn Val Wing Wing Gly Val Glu Leu Thr Pro

485 490 495

Pro Asn Glu Pro Arg Arg Read Leu Be Wing Arg Be Thr Be Lys Leu

500 505 510

Be Ser Pro Lys Asn Val Wing Be Ser Cys Wing Trp Be Ser Asn Cys Wing

515 520 525

Leu Wing Ser Lys Leu Val Ser Ser Ile Pro Wing Wing Leu Ile Wing Gly

530 535 540

Val Ile Glu Arg Wing Arg Glu Gly Asp Cys Asp Cys Glu Pro Asp Ala545 550 555 560

Pro Arg Glu Met Cys Leu Wing Leu Asn Wing Asn Wing Asn Gly Asp Wing

565 570 575

Gly Wing Thr Wing Pro Ile Wing Gly Leu Asn Ser Glu Ile Gly Val Pro

580 585 590

Gly Val Pro Gly Val Pro Gly Glu Pro Ile Arg Read Leu Read Leu Cys Wing

595 600 605

Asn Pro Leu Leu610 <210> 66 <211> 395 <212> PRT

<213> Saccharomyces cereviseae <400> 66

Met Asp Pro Thr Lys Pro Asp Phe Lys Pro Pro Gln Pro Asn Glu1 5 10 15

Glu Leu Gln Pro Pro Pro Asp Pro His Thr Ile Pro Lys Ser Gly

20 25 30

Pro Ile Val Pro Tyr Val Leu Wing Asp Tyr Asn Be Ser Ile Asp Ala35 40 45Pro Phe Asn Read Asp Ile Tyr Lys Thr Leu Be Ser Arg Lys Lys Asn

50 55 60

Wing Asn Ser Be Asn Arg Met Asp His Ile Pro Read Asn Thr Be Asp65 70 75 80

Phe Gln Pro Read Be Arg Asp Val Be Be Glu Glu Glu Be Glu Gly

85 90 95

Gln Be Asn Gly Ile Asp Wing Thr Read Gln Asp Val Thr Met Thr Gly

100 105 110

Asn Leu Gly Val Leu Lys Ser Gln Ile Wing Asp Leu Glu Glu Val Pro

115 120 125

His Thr Ile Val Arg Gln Wing Arg Thr Ile Glu Asp Tyr Glu Phe Pro

130 135 140

Val His Arg Leu Thr Lys Lys Leu Gln Asp Pro Glu Lys Leu Pro Leu145 150 155 160

Ile Ile Val Cys Wing Gly Ser Phe Ser Pro Ile Thr Tyr Leu His Leu

165 170 175

Arg Met Phe Glu Met Wing Read Asp Asp Ile Asn Glu Gln Thr Arg Phe

180 185 190

Glu Val Val Gly Gly Gly Tyr Phe Ser Pro Val Ser Asp Asn Tyr Gln Lys

195 200 205

Arg Gly Leu Wing Pro Wing Tyr His Arg Val Arg Met Cys Glu Leu Wing

210 215 220

Cys Glu Arg Thr Be Trp Leu Met Val Asp Wing Trp Glu Be Leu225 230 235 240

Gln Be Ser Tyr Thr Arg Thr Wing Lys Val Leu Asp His Phe Asn His

245 250 255

Glu Ile Asn Ile Lys Arg Gly Gly Ile Met

260 265 270

Met Gly Val Lys Ile Met Leu Read Leu Wing Gly Gly Asp Leu Ile Glu Ser

275 280 285

Met Gly Glu Pro His Val Trp Wing Asp Ser Asp Leu His His Ile Leu290 295 300Gly Asn Tyr Gly Cys Leu Ile Val305 310

Be Phe Leu Read Be His Asp Ile325

Ile Ile Ile Lys Gln Ile Ile Tyr340

Arg Read Phe Ile Arg Arg Gly Met355 360

Ser Val Ile Arg Tyr Ile Gln Glu

370 375

Glu Pro Val Lys Gln Val Leu Asp385 390

Glu Arg Thr Gly Ser Asp Val Arg315 320

Met Tyr Glu His Arg Arg Asn Ile

330 335

Asn Asp Ile Be Ser Thr Lys Val345 350

Ser Val Gln Tyr Leu Leu Pro Asn365

Tyr Asn Leu Tyr Ile Asn Gln Ser380

Ser Lys Glu395

Claims (10)

A method of obtaining a plant with increased fatigue resistance comprising: (a) introducing a chimeric gene into a plant cell to obtain transgenic cells, said chimeric gene comprising the following fragments of Operably linked DNA: (i) a passive plant expression promoter, (ii) a DNA region encoding a functional enzyme depletes the nicotine mida adenine dinucleotide recovery synthesis pathway selected from nicotinamidase, nicotinate phosphorylbosyltransferase rase, adenyl transferase nicotinic acid mononucleotide or nicotinamide adenine synthetase dinucleo-tide (iii) a 3 'terminal region involved in transcription, termination and polyadenylation, (b) regeneration of said transgenic cells to obtain a transgenic plant population; selection of a plant from said transgenic plant population that exhibits increased fatigue resistance or selection of a plant that exhibits a low level of reactive oxygen species or maintains a high level of NADH under fatigue conditions when compared to a similar non-transgenic plant, characterized in that the amino acid sequence encoded by the DNA region comprises the amino acid sequence of any of SEQ IDs No. 1 to 29, 31 to 47 or 49 to 66 or an amino acid sequence having at least 90% less sequence identity with said amino acid sequences of any of SEQ IDs No. 1 to 29, 31 to 47 or 49 to 66. Chimeric gene as defined in claim 1. Plant cell comprising a chimeric gene as described in claim 2. 4. Plant comprising a chimeric gene as defined in claim 2. Plant according to Claim 4, characterized in that it has a lower level of oxygen-reactive species under conditions of fatigue than a similar plant which does not comprise such a chimeric gene. Plant seed as defined in claim 4 or 5, comprising a chimeric gene as defined in claim 2. Use of a chimeric gene as defined in claim 2 to increase plant fatigue strength. Use of chimeric genes as defined in claim 2 to lower the level of oxygen reactive species in a plant or plant cell under conditions of fatigue or to maintain the level of NAD in a plant or plant cell under conditions. Fatigue 9. Use of a DNA sequence encoding a plant functional enzyme in the nicotinamide adenine dinucleotide recovery synthesis pathway selected from nicotinamidase, nicotinate phosphoryl bosyltransferase, nicotinic acid mononucleotide transferase or nicotinamide dinucleotide adenine synthetase to fatigue resistance of a plant, characterized in that said plant functional enzyme is derived from a fungus or yeast-like organism other than Saccharomyces cerevisae. 10. Use of a DNA sequence encoding a plant functional enzyme in the nicotinamide adenine dinucleotide recovery synthesis pathway selected from nicotinamidase, nicotinate phosphoryl bosyltransferase, nicotinic acid mononucleotide transferase or nicotinamide adenine dinucleotide to decrease synthetase level of reactive oxygen species in a plant or a plant cell under conditions of fatigue or to maintain the level of NAD in a plant or a plant cell under conditions of fatigue, characterized in that said plant functional enzyme is derived from a fungus or yeast-like organism other than Saceharomyces cerevisae.