BRPI0710949A2 - Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos - Google Patents

Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos Download PDF

Info

Publication number
BRPI0710949A2
BRPI0710949A2 BRPI0710949-0A BRPI0710949A BRPI0710949A2 BR PI0710949 A2 BRPI0710949 A2 BR PI0710949A2 BR PI0710949 A BRPI0710949 A BR PI0710949A BR PI0710949 A2 BRPI0710949 A2 BR PI0710949A2
Authority
BR
Brazil
Prior art keywords
pluripotent stem
stem cells
period
cardiomyocytes
canonical wnt
Prior art date
Application number
BRPI0710949-0A
Other languages
English (en)
Inventor
Koshimizu Uichi
Tanaka Tomofumi
Kawashima Kayoko
Kadokura Michinori
Original Assignee
Asubio Pharma Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asubio Pharma Co., Ltd. filed Critical Asubio Pharma Co., Ltd.
Publication of BRPI0710949A2 publication Critical patent/BRPI0710949A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODO PARA INDUÇçO DE DIFERENCIAÇçO DE CÉLULAS TRONCO PLURIPOTENTES EM CARDIOMIàCITOS. A presente invenção fornece um método para a indução de diferenciação em cardiomiácitos, de maneira eficiente e seletiva, a partir de células tronco. Um método para indução de diferenciação em cardiomiácitos a partir de células tronco pluripotentes, que compreende: (i) o cultivo das células tronco pluripotentes em um meio de cultura não contendo qualquer substância que promova a ativação da via de sinalização Wnt canônica durante o período de tempo entre o início da indução de diferenciação e 24 horas antes do período de elevada expressão de gene Wnt canônico; e, então, (ii) o cultivo das células tronco pluripotentes em um meio de cultura contendo uma substância, que promova a ativação da via de sinalização Wnt canônica durante um período de tempo de 24 a 96 horas, partindo de 24 a O horas antes do período de elevada expressão de gene Wnt canônico.
BRPI0710949-0A 2006-04-28 2007-04-27 Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos BRPI0710949A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2006125148 2006-04-28
JP2006-125148 2006-04-28
JP2007019531 2007-01-30
JP2007-019531 2007-01-30
PCT/JP2007/059242 WO2007126077A1 (ja) 2006-04-28 2007-04-27 多能性幹細胞から心筋細胞を分化誘導する方法

Publications (1)

Publication Number Publication Date
BRPI0710949A2 true BRPI0710949A2 (pt) 2012-03-06

Family

ID=38655597

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0710949-0A BRPI0710949A2 (pt) 2006-04-28 2007-04-27 Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos

Country Status (11)

Country Link
US (1) US8293529B2 (pt)
EP (2) EP2014766A4 (pt)
JP (1) JP5149791B2 (pt)
KR (2) KR101234544B1 (pt)
CN (1) CN101426902B (pt)
AU (1) AU2007244226B2 (pt)
BR (1) BRPI0710949A2 (pt)
CA (1) CA2650685C (pt)
IL (1) IL194970A (pt)
RU (1) RU2433174C2 (pt)
WO (1) WO2007126077A1 (pt)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110243899A1 (en) * 2008-04-25 2011-10-06 National University Corporation Chiba University Wnt Signaling Inhibitor Comprising Insulin-Like Growth Factor-Binding Protein
WO2008060446A2 (en) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Methods for inducing cardiomyogenesis
WO2010007031A2 (en) * 2008-07-14 2010-01-21 Novartis Ag Methods for improving cardiac differentiation of human embryonic stem cells
EP2490701B9 (en) 2009-10-19 2017-11-15 Cellular Dynamics International, Inc. Cardiomyocyte production
US20120244619A1 (en) * 2009-12-09 2012-09-27 Kyoto University Composition for promoting differentiation of pluripotent stem cells into cardiac muscle cells which comprises nitrovin
EP2412724A1 (en) * 2010-07-29 2012-02-01 Centre National de la Recherche Scientifique (C.N.R.S) Regulation of Glypican 4 activity to modulate the fate of stem cells and uses thereof
US9499790B2 (en) 2010-08-26 2016-11-22 Kyoto University Method for promoting differentiation of pluripotent stem cells into cardiac muscle cells
EP2610249B1 (en) * 2010-08-26 2017-10-11 Kyoto University Pluripotent stem cell cardiomyocyte differentiation-promoting agent
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
JP6185907B2 (ja) 2011-03-30 2017-08-23 セルラー ダイナミクス インターナショナル， インコーポレイテッド 神経分化のための多能性幹細胞の予備刺激
JP5840855B2 (ja) 2011-03-30 2016-01-06 学校法人東京女子医科大学 胚性幹細胞から心筋シートを製造する方法
WO2013013206A1 (en) 2011-07-21 2013-01-24 The Board Of Trustees Of The Leland Stanford Junior University Cardiomyocytes from induced pluripotent stem cells from patients and methods of use
KR101996343B1 (ko) 2012-01-27 2019-07-05 고쿠리츠 다이가쿠 호진 교토 다이가쿠 전능성 줄기세포의 심근분화 유도방법
US9764063B2 (en) * 2012-03-15 2017-09-19 Iheart Japan Corporation Method for producing mixed cell population of cardiomyocytes and vascular cells from induced pluripotent stem cell
WO2013159349A1 (en) * 2012-04-27 2013-10-31 Curegenix Inc. Method for producing cardiomyocytes
WO2014136519A1 (ja) 2013-03-08 2014-09-12 国立大学法人京都大学 Ｅｇｆ受容体阻害剤を含む多能性幹細胞の心筋分化促進剤
US9493742B2 (en) 2013-03-15 2016-11-15 Emory University Purification of cell mixtures using molecular beacons targeting cell specific RNA
CN105247041B (zh) 2013-05-31 2018-04-20 爱心细胞有限公司 组入有水凝胶的层叠细胞膜片
EP3043822A1 (en) * 2013-09-11 2016-07-20 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Compositions for preparing cardiomyocytes
RU2537141C1 (ru) * 2013-11-20 2014-12-27 государственное бюджетное образовательное учреждение высшего профессионального образования "Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова" Министерства здравоохранения Российской Федерации Способ определения способности клеток костного мозга к делению
JP6478116B2 (ja) 2013-12-25 2019-03-06 東亞合成株式会社 多能性幹細胞から内胚葉系細胞への分化誘導方法
CN105039399A (zh) * 2014-04-23 2015-11-11 复旦大学 多能干细胞-遗传性心肌病心肌细胞及其制备方法
EP3150705B1 (en) 2014-05-30 2019-05-15 Kyoto University Method for inducing myocardial differentiation of pluripotent stem cells using low-molecular compound
JP6487654B2 (ja) * 2014-09-02 2019-03-20 国立大学法人 東京大学 多能性幹細胞から心筋細胞を分化誘導する方法、並びに該方法に好適な培地添加剤、分化誘導調節剤、培地、培地作製用キット、及び多能性幹細胞から心筋細胞を分化誘導するためのキット
EP3286301B1 (en) * 2015-04-22 2021-07-28 Sonic Master Limited Generation of muscle-lineage cells from stem cells
JP6935654B2 (ja) 2015-04-28 2021-09-15 東亞合成株式会社 合成ペプチドを用いた心筋細胞の生産方法
JP6691756B2 (ja) 2015-09-29 2020-05-13 東亞合成株式会社 合成ペプチドを用いた神経幹細胞の生産方法
WO2018034519A1 (ko) * 2016-08-18 2018-02-22 서울대학교병원 Necrox를 이용한 줄기세포 유래 심근세포의 분화 효율 및 성숙도 증진 방법
US11459546B2 (en) * 2016-09-30 2022-10-04 Kyoto Prefectural Public University Corporation Method for producing somatic cell, somatic cell, and composition
KR102010922B1 (ko) * 2017-11-01 2019-08-14 가톨릭관동대학교산학협력단 켄파울론 유도체의 줄기세포의 심근세포로의 분화 유도 용도
EP3778869A4 (en) 2018-03-30 2021-12-08 Kyoto University CARDIOMYOCYTE MATURATION PROMOTOR
JP7257698B2 (ja) 2018-03-30 2023-04-14 オリヅルセラピューティクス株式会社 複素環化合物
JP7440868B2 (ja) 2018-03-30 2024-02-29 国立大学法人京都大学 細胞の製造方法
JP7436990B2 (ja) 2018-08-10 2024-02-22 国立大学法人京都大学 カチオン性脂質を用いた心筋細胞へのトランスフェクション方法
CN110907644B (zh) * 2019-12-11 2023-01-06 深圳市达科为生物工程有限公司 多种细胞鉴定试剂盒及操作方法
CN111411075A (zh) * 2020-03-10 2020-07-14 广东源心再生医学有限公司 一种构建人心肌细胞凋亡模型的方法
EP4159846A4 (en) 2020-05-26 2024-08-14 Healios K.K. HYPOIMMUNOGENIC CELLS
KR102205522B1 (ko) 2020-09-28 2021-01-19 이상호 미닫이형 자동문
CN114591895B (zh) * 2022-04-12 2025-07-22 澳门大学 一种诱导干细胞分化为心肌细胞的方法及其培养基组合
CN114891729B (zh) * 2022-04-15 2024-04-05 北京全式金生物技术股份有限公司 叶酸在促进人多能性干细胞向心肌细胞分化中的应用
AU2024232721A1 (en) 2023-03-03 2025-10-09 Kyoto University Method for producing primed pluripotent stem cells

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL47012C (pt) 1933-03-23
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US6015671A (en) * 1995-06-07 2000-01-18 Indiana University Foundation Myocardial grafts and cellular compositions
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
WO1999027076A1 (en) 1997-11-25 1999-06-03 Arc Genomic Research Pluripotent embryonic stem cells and methods of obtaining them
WO2000027995A1 (en) 1998-11-09 2000-05-18 Monash University Embryonic stem cells
GB9903804D0 (en) 1999-02-20 1999-04-14 Univ Sheffield Pluripotential cells-2
AU2001297880B2 (en) 2000-11-30 2007-05-31 Stemron Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
WO2003006950A2 (en) 2001-07-12 2003-01-23 Geron Corporation Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
US20030232430A1 (en) * 2001-11-26 2003-12-18 Advanced Cell Technology Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells
US20040014209A1 (en) * 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
CN1536076A (zh) * 2003-04-09 2004-10-13 中国人民解放军军事医学科学院野战输 成年人骨髓间充质干细胞体外扩增和定向诱导分化为心肌样细胞的方法
AU2004278634B2 (en) 2003-10-03 2009-10-01 Keiichi Fukuda Method of inducing the differentiation of stem cells into cardiomyocytes
JP2005224155A (ja) 2004-02-12 2005-08-25 Yamaguchi Technology Licensing Organization Ltd 舌組織から単離された、自動拍動する心筋細胞に分化する能力を有する細胞および細胞の培養、分化誘導法
WO2005118782A2 (en) 2004-04-16 2005-12-15 Hydra Biosciences, Inc. Methods of promoting cardiac cell proliferation
KR101318713B1 (ko) 2004-08-27 2013-10-16 각고호우징 게이오기주크 세포내 미토콘드리아를 지표로 이용하는 심근 세포의 선별방법
JP4200499B2 (ja) 2005-08-17 2008-12-24 日本ビクター株式会社 音声符号化方法及び音声復号装置

Also Published As

Publication number Publication date
CA2650685A1 (en) 2007-11-08
EP2014766A1 (en) 2009-01-14
KR101346047B1 (ko) 2013-12-31
IL194970A0 (en) 2011-08-01
WO2007126077A1 (ja) 2007-11-08
RU2008146991A (ru) 2010-06-10
AU2007244226A1 (en) 2007-11-08
IL194970A (en) 2012-05-31
AU2007244226B2 (en) 2012-09-20
US20090325288A1 (en) 2009-12-31
CN101426902B (zh) 2013-03-27
KR20090009267A (ko) 2009-01-22
CN101426902A (zh) 2009-05-06
RU2433174C2 (ru) 2011-11-10
EP2014766A4 (en) 2009-12-02
KR20120139856A (ko) 2012-12-27
US8293529B2 (en) 2012-10-23
CA2650685C (en) 2014-02-04
JP5149791B2 (ja) 2013-02-20
JPWO2007126077A1 (ja) 2009-09-10
EP2457994A1 (en) 2012-05-30
KR101234544B1 (ko) 2013-03-07

Similar Documents

Publication Publication Date Title
BRPI0710949A2 (pt) Método para indução de diferenciação de células tronco pluripotentes em cardiomiócitos
MX2010002426A (es) Agente para promover la adhesion de las celulas del endotelio corneal.
Dong et al. Depletion of histone demethylase KDM2A enhanced the adipogenic and chondrogenic differentiation potentials of stem cells from apical papilla
WO2009023525A3 (en) Methods of modulating mesenchymal stem cell differentiation
BR112022017216A2 (pt) Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes
EP2410043A3 (en) Stem cells culture systems
NZ619359A (en) Method of producing erythrocytes without feeder cells
WO2008036447A3 (en) Pluripotent stem cell culture
MY171901A (en) Process of separating components of a fermentation broth
BRPI0922572A2 (pt) método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
SE0850111L (sv) Förfarande för differentiering av stamceller till celler av den endodermala och pankreatiska utvecklingslinjen
MX356756B (es) Células madre pluripotentes en microportadores.
EA201000378A1 (ru) Способ получения эндотелиальных клеток (варианты)
EP2896690A3 (en) Use of RNA for reprogramming somatic cells
WO2007115337A8 (en) Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering
GB2441718A (en) Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
AU2017254264A1 (en) Method for producing retinal tissue
CN114480259B (zh) 一种诱导小鼠全能样干细胞的培养基及诱导方法
BR112014006244A2 (pt) microrganismos e métodos para produzir alcenos
BRPI0506369A (pt) microorganismos, e, métodos para produzir um l-aminoácido
RU2011121843A (ru) Дифференцирование человеческих эмбриональных стволовых клеток в линию панкреатических эндокринных клеток
SG10201808863UA (en) Combined chemical and genetic approaches for generation of induced pluripotent stem cells
Xing et al. A comprehensive study on donor‐matched comparisons of three types of mesenchymal stem cells‐containing cells from human dental tissue
BR112012009828A8 (pt) método para a produção de uma imunoglobulina glicosilada
NZ591812A (en) Cell culture method to form aggregates

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: DAIICHI SANKYO COMPANY, LIMITED (JP)

Free format text: TRANSFERIDO POR INCORPORACAO DE: ASUBIO PHARMA CO., LTD.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]