BRPI0813729A2 - Método para construção de massas de células miocárdicas e uso da massa de célula miocárdica - Google Patents

Método para construção de massas de células miocárdicas e uso da massa de célula miocárdica

Info

Publication number
BRPI0813729A2
BRPI0813729A2 BRPI0813729-3A2A BRPI0813729A BRPI0813729A2 BR PI0813729 A2 BRPI0813729 A2 BR PI0813729A2 BR PI0813729 A BRPI0813729 A BR PI0813729A BR PI0813729 A2 BRPI0813729 A2 BR PI0813729A2
Authority
BR
Brazil
Prior art keywords
myocardic
cell
construction
masses
cell mass
Prior art date
Application number
BRPI0813729-3A2A
Other languages
English (en)
Inventor
Fumiyuki Hattori
Keiichi Fukuda
Original Assignee
Asubio Pharma Co Ltd
Univ Keio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asubio Pharma Co Ltd, Univ Keio filed Critical Asubio Pharma Co Ltd
Publication of BRPI0813729A2 publication Critical patent/BRPI0813729A2/pt
Publication of BRPI0813729B1 publication Critical patent/BRPI0813729B1/pt
Publication of BRPI0813729B8 publication Critical patent/BRPI0813729B8/pt

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3826—Muscle cells, e.g. smooth muscle cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/99—Serum-free medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Epidemiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Cardiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Botany (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Heart & Thoracic Surgery (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
BRPI0813729A 2007-07-31 2008-07-31 métodos de preparação de massas celulares de cardiomiócitos purificados derivados de células-tronco pluripotentes e de uma película de massas celulares de cardiomiócitos, bem como dispositivo médico e uso das referidas massas celulares BRPI0813729B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2007-200246 2007-07-31
JP2007200246 2007-07-31
JP2008-046772 2008-02-27
JP2008046772 2008-02-27
PCT/JP2008/064168 WO2009017254A1 (ja) 2007-07-31 2008-07-31 心筋細胞の細胞塊作製方法及び当該心筋細胞塊の用途

Publications (3)

Publication Number Publication Date
BRPI0813729A2 true BRPI0813729A2 (pt) 2014-12-30
BRPI0813729B1 BRPI0813729B1 (pt) 2019-04-02
BRPI0813729B8 BRPI0813729B8 (pt) 2022-09-27

Family

ID=40304474

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0813729A BRPI0813729B8 (pt) 2007-07-31 2008-07-31 métodos de preparação de massas celulares de cardiomiócitos purificados derivados de células-tronco pluripotentes e de uma película de massas celulares de cardiomiócitos, bem como dispositivo médico e uso das referidas massas celulares

Country Status (11)

Country Link
US (2) US9109205B2 (pt)
EP (1) EP2172542B1 (pt)
JP (1) JP5523830B2 (pt)
KR (2) KR20100040276A (pt)
CN (1) CN101711277B (pt)
AU (1) AU2008283255B2 (pt)
BR (1) BRPI0813729B8 (pt)
CA (1) CA2690610C (pt)
IL (1) IL203404A (pt)
RU (1) RU2467066C2 (pt)
WO (1) WO2009017254A1 (pt)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011040469A1 (ja) * 2009-09-30 2011-04-07 国立大学法人鳥取大学 新規ペースメーカ細胞
JP5902092B2 (ja) * 2009-10-19 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド 心筋細胞の生成
EP2511366A4 (en) * 2009-12-09 2014-04-30 Univ Kyoto NITROVINE MEDICINE FOR PROMOTING THE DIFFERENTIATION OF PLURIPOTENTAL STEM CELLS IN HEART MUSCLES
EP2582792B1 (en) * 2010-06-18 2018-10-31 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte medium with dialyzed serum
JP5854999B2 (ja) * 2010-07-29 2016-02-09 テルモ株式会社 シート状細胞培養物解離システム
WO2015175534A2 (en) * 2014-05-13 2015-11-19 The Regents Of The University Of Michigan Compositions and methods employing stem cell-derived cardiomyocytes
SG11201700023WA (en) 2014-07-16 2017-02-27 Heartseed Inc New undifferentiated stem cell removal and myocardial purification and refinement culture medium
EP3202896B1 (en) * 2014-09-30 2019-05-15 JTEC Corporation Method for culturing pluripotent stem cells
EP3219790B1 (en) * 2014-11-12 2021-08-04 Terumo Kabushiki Kaisha Myocardial cell sheet
CN104830761A (zh) * 2015-06-04 2015-08-12 黑龙江省瑞生会生物技术开发有限公司 一种培养心肌细胞的无血清培养基
EP3150696A1 (en) 2015-09-30 2017-04-05 Shibuya Corporation Apparatus for producing cell mass sheet and method for producing cell mass sheet
JP6593589B2 (ja) * 2015-09-30 2019-10-23 澁谷工業株式会社 細胞塊シートの作製方法および細胞塊シート
EP3162890A1 (en) 2015-10-29 2017-05-03 Shibuya Corporation Method and apparatus for producing cell mass structure
WO2017159862A1 (ja) * 2016-03-18 2017-09-21 国立大学法人京都大学 多能性幹細胞由来心筋細胞の凝集体の凍結方法
JP6811489B2 (ja) 2016-10-17 2021-01-13 学校法人慶應義塾 未分化幹細胞除去剤、及び未分化幹細胞除去方法
SG11201805456WA (en) 2016-11-01 2019-05-30 Terumo Corp Modification method for sheet-shaped cell culture
JP2018093823A (ja) 2016-12-15 2018-06-21 Ｈｅａｒｔｓｅｅｄ株式会社 未分化幹細胞除去剤及び未分化幹細胞除去方法
WO2018164098A1 (ja) * 2017-03-06 2018-09-13 国立大学法人大阪大学 心臓線維化モデルとして用いられる細胞集団、その製造方法、それを利用したスクリーニング方法及び心臓線維化モデルの評価方法、並びに心臓線維化モデルの評価システム
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
JPWO2018164098A1 (ja) * 2017-03-16 2020-01-16 国立大学法人大阪大学 心臓線維化モデルとして用いられる細胞集団、その製造方法、それを利用したスクリーニング方法及び心臓線維化モデルの評価方法、並びに心臓線維化モデルの評価システム
JP7298844B2 (ja) 2017-06-05 2023-06-27 テルモ株式会社 細胞培養物の製造方法
WO2020009188A1 (ja) * 2018-07-06 2020-01-09 株式会社マイオリッジ 細胞シートの製造方法、心筋細胞シート、および心筋細胞シート製造用キット
US20220389355A1 (en) * 2019-11-21 2022-12-08 The Japan Wool Textile Co., Ltd. Cell aggregate, producing method for manufacturing cell aggregate, producing kit for cell aggregate, and chemical compound evaluating method using cell aggregate
KR102188256B1 (ko) * 2019-12-02 2020-12-08 주식회사 티앤알바이오팹 동결보관 및 저산소 조건에서의 생존율을 향상시키는 심근세포 응집체 제작 기술
US20230287349A1 (en) 2020-07-14 2023-09-14 Kaneka Corporation Method for producing cardiomyocyte spheroids
CN112657051B (zh) * 2020-12-25 2023-08-04 上海交通大学医学院附属上海儿童医学中心 一种基于共轭同轴静电纺丝技术的生物型心室辅助泵及其制备方法
JPWO2022210824A1 (pt) * 2021-03-31 2022-10-06

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0372872A (ja) * 1988-07-12 1991-03-28 Bio Kagaku Kenkyusho:Kk 無血清培地及びそれを用いた哺乳類細胞の培養法
EP1543110A4 (en) * 2002-05-17 2006-09-06 Sinai School Medicine POPULATION OF DEFINITIVE ENDODERMIC CELLS AND MESODERMAL
AU2004278634B2 (en) * 2003-10-03 2009-10-01 Keiichi Fukuda Method of inducing the differentiation of stem cells into cardiomyocytes
JP4971131B2 (ja) * 2004-03-19 2012-07-11 ジェロン・コーポレーション 再生医学に適した高純度心筋細胞調製物を作製するための方法
US7452718B2 (en) * 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US20050214938A1 (en) * 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
EP1785482B1 (en) * 2004-08-27 2017-10-04 Daiichi Sankyo Company, Limited A method of selecting a cardiomyocyte using intracellular mitochondria as an indicator

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WO2009017254A1 (ja) 2009-02-05
US10918671B2 (en) 2021-02-16
CN101711277A (zh) 2010-05-19
CA2690610A1 (en) 2009-02-05
BRPI0813729B1 (pt) 2019-04-02
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CA2690610C (en) 2023-03-28
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EP2172542A4 (en) 2011-12-21
BRPI0813729B8 (pt) 2022-09-27
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EP2172542B1 (en) 2017-03-22
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KR20130090423A (ko) 2013-08-13
US20100189699A1 (en) 2010-07-29
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