BRPI0819929A2 - Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica - Google Patents

Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica

Info

Publication number
BRPI0819929A2
BRPI0819929A2 BRPI0819929-9A2A BRPI0819929A BRPI0819929A2 BR PI0819929 A2 BRPI0819929 A2 BR PI0819929A2 BR PI0819929 A BRPI0819929 A BR PI0819929A BR PI0819929 A2 BRPI0819929 A2 BR PI0819929A2
Authority
BR
Brazil
Prior art keywords
live
expression
pichia pastoris
acid amino
methylotrophic yeast
Prior art date
Application number
BRPI0819929-9A2A
Other languages
English (en)
Inventor
Travis Young
Peter G Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0819929A2 publication Critical patent/BRPI0819929A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0819929-9A2A 2007-12-11 2008-12-10 Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica BRPI0819929A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US734107P 2007-12-11 2007-12-11
PCT/US2008/013568 WO2009075847A2 (en) 2007-12-11 2008-12-10 In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris

Publications (1)

Publication Number Publication Date
BRPI0819929A2 true BRPI0819929A2 (pt) 2014-10-14

Family

ID=40756044

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0819929-9A2A BRPI0819929A2 (pt) 2007-12-11 2008-12-10 Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica

Country Status (11)

Country Link
US (2) US8647840B2 (pt)
EP (2) EP2231856A4 (pt)
JP (3) JP2011527886A (pt)
KR (1) KR20100105846A (pt)
CN (2) CN101939410A (pt)
AU (1) AU2008335778B2 (pt)
BR (1) BRPI0819929A2 (pt)
CA (1) CA2708760A1 (pt)
IL (2) IL206300A (pt)
RU (1) RU2010125104A (pt)
WO (1) WO2009075847A2 (pt)

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JP2011527886A (ja) 2007-12-11 2011-11-10 ザ スクリプス リサーチ インスティチュート メタノール資化性酵母ピキア・パストリスにおけるインビボ非天然アミノ酸発現
WO2010053636A1 (en) * 2008-11-06 2010-05-14 Tate & Lyle Technology Ltd Enhanced production and purification of a natural high intensity sweetener
EP2370473B1 (en) * 2008-12-10 2016-05-11 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
CN103145845A (zh) * 2011-12-06 2013-06-12 吉林圣元科技有限责任公司 Tat与人野生型P53融合蛋白在毕赤酵母中的表达
CN103060411B (zh) * 2013-01-05 2014-02-05 义马煤业集团煤生化高科技工程有限公司 一种甲醇蛋白肽的生产方法
WO2014145521A2 (en) * 2013-03-15 2014-09-18 Alderbio Holdings Llc Fermentation process for antibody production
CN104312932B (zh) * 2014-10-24 2018-03-27 北京科兴中维生物技术有限公司 一种甲醇营养型酵母的蛋白诱导方法
US11008370B2 (en) * 2015-10-26 2021-05-18 Council Of Scientific & Industrial Research Genetically modified yeast cell and improved process for production of clot-specific streptokinase
CN108368499A (zh) * 2015-11-30 2018-08-03 欧洲分子生物学实验室 通过在昆虫细胞中扩增遗传密码来制备工程化蛋白质的手段和方法
CN106770866A (zh) * 2015-12-17 2017-05-31 中国医科大学 可实现人血清白蛋白鉴定及绝对定量的试剂盒及测定方法
CN105385693B (zh) * 2015-12-25 2018-09-18 安阳工学院 一种高效表达多拷贝人表皮生长因子的方法
CN112142846B (zh) * 2020-10-10 2022-07-08 西北大学 Aox特异性抗体组合及其制备方法和用途
CN112940956A (zh) * 2021-04-18 2021-06-11 江南大学 采用双启动子增强乳铁蛋白表达的毕赤酵母及其应用
CN119913187A (zh) * 2023-10-31 2025-05-02 康码（上海）生物科技有限公司 一种基于tRNA改造的基因工程菌株及其制备方法和应用

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ES2464532T3 (es) * 2001-04-19 2014-06-03 The Scripps Research Institute Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
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JP5275566B2 (ja) * 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
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Also Published As

Publication number Publication date
IL206300A0 (en) 2010-12-30
US20110014650A1 (en) 2011-01-20
WO2009075847A3 (en) 2009-09-11
JP2014039554A (ja) 2014-03-06
US9732349B2 (en) 2017-08-15
US8647840B2 (en) 2014-02-11
WO2009075847A2 (en) 2009-06-18
JP2011527886A (ja) 2011-11-10
IL206300A (en) 2014-07-31
KR20100105846A (ko) 2010-09-30
EP2231856A2 (en) 2010-09-29
JP2016144469A (ja) 2016-08-12
US20140287464A1 (en) 2014-09-25
CA2708760A1 (en) 2009-06-18
IL228238A (en) 2014-09-30
CN105647824A (zh) 2016-06-08
JP6073205B2 (ja) 2017-02-01
EP2231856A4 (en) 2011-05-18
EP2889370A1 (en) 2015-07-01
AU2008335778A1 (en) 2009-06-18
RU2010125104A (ru) 2012-01-20
CN101939410A (zh) 2011-01-05
AU2008335778B2 (en) 2014-04-10

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 5A E 6A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]