BRPI1006435A8 - Molécula de ácido nucleico recombinante e célula hospedeira microbiana - Google Patents

Molécula de ácido nucleico recombinante e célula hospedeira microbiana

Info

Publication number
BRPI1006435A8
BRPI1006435A8 BRPI1006435A BRPI1006435A BRPI1006435A8 BR PI1006435 A8 BRPI1006435 A8 BR PI1006435A8 BR PI1006435 A BRPI1006435 A BR PI1006435A BR PI1006435 A BRPI1006435 A BR PI1006435A BR PI1006435 A8 BRPI1006435 A8 BR PI1006435A8
Authority
BR
Brazil
Prior art keywords
nucleic acid
host cell
acid molecule
recombinant nucleic
microbial host
Prior art date
Application number
BRPI1006435A
Other languages
English (en)
Inventor
E Apt Kirk
Simpson David
Richter Leslie
Zirkle Ross
Original Assignee
Martek Biosciences Corp
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Biosciences Corp, Dsm Ip Assets Bv filed Critical Martek Biosciences Corp
Publication of BRPI1006435A2 publication Critical patent/BRPI1006435A2/pt
Publication of BRPI1006435A8 publication Critical patent/BRPI1006435A8/pt
Publication of BRPI1006435B1 publication Critical patent/BRPI1006435B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01039—[Acyl-carrier-protein] S-malonyltransferase (2.3.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01059—3-Hydroxyacyl-[acyl-carrier-protein] dehydratase (4.2.1.59)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Obesity (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

MOLÉCULA DE ÁCIDO NUCLEICO RECOMBINANTE E CÉLULA HOSPEDEIRA MICROBIANA. A presente invenção se refere a moléculas de ácido nucleico isoladas e polipeptídeos das sintases de ácidos graxos poli-insaturados (AGPl) de traustocitridios envolvidas na produção de AGPls, incluindo AGPls enriquecidos com ácido docosaexaenoico (DHA), ácido eicosapentaenoico (EPA), ou uma combinação destes. A presente invenção está direcionada para vetores e células hospedeiras compreendendo as moléculas de ácido nucleico, polipeptídeos codificados pelas moléculas de ácido nucleico, composições compreendendo as moléculas de ácido nucleico ou polipeptídeos, e métodos de produção e uso dos mesmos.
BRPI1006435-4A 2009-03-19 2010-03-19 molécula de ácido nucleico recombinante e célula hospedeira microbiana BRPI1006435B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16174209P 2009-03-19 2009-03-19
US61/161,742 2009-03-19
US29646010P 2010-01-19 2010-01-19
US61/296,460 2010-01-19
PCT/US2010/028009 WO2010108114A2 (en) 2009-03-19 2010-03-19 Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof

Publications (3)

Publication Number Publication Date
BRPI1006435A2 BRPI1006435A2 (pt) 2016-10-04
BRPI1006435A8 true BRPI1006435A8 (pt) 2017-09-26
BRPI1006435B1 BRPI1006435B1 (pt) 2021-01-19

Family

ID=42740260

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1006435-4A BRPI1006435B1 (pt) 2009-03-19 2010-03-19 molécula de ácido nucleico recombinante e célula hospedeira microbiana

Country Status (11)

Country Link
US (4) US8940884B2 (pt)
EP (2) EP2408797B1 (pt)
JP (3) JP2012521195A (pt)
CN (1) CN102741267B (pt)
AR (1) AR075897A1 (pt)
AU (1) AU2010226440B2 (pt)
BR (1) BRPI1006435B1 (pt)
CA (2) CA2755639C (pt)
ES (2) ES2717102T3 (pt)
TW (1) TWI600762B (pt)
WO (1) WO2010108114A2 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
EP1707055A3 (en) 2000-01-28 2009-01-14 Martek Biosciences Corporation Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors
US20070243304A1 (en) * 2004-05-24 2007-10-18 Mahabaleshwar Hedge Omega-3 Fatty Acid Compositions With Honey
ES2717102T3 (es) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
CA2993690C (en) 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
TW201144442A (en) * 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
AU2015252107B2 (en) * 2011-03-07 2018-01-18 Dsm Nutritional Products Ag Engineering thraustochytrid microorganisms
US9133463B2 (en) 2011-03-07 2015-09-15 Dsm Nutritional Products Ag Engineering microorganisms
MX342216B (es) * 2011-07-21 2016-09-20 Dsm Ip Assets Bv Composiciones de acidos grasos.
EP2734626B1 (en) * 2011-07-21 2020-12-16 DSM IP Assets B.V. Microbial oils enriched in polyunsaturated fatty acids
TW201525136A (zh) * 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌PUFA合成酶於油籽作物中生成ω-3長鏈多不飽和脂肪酸
ES2721269T3 (es) 2014-01-28 2019-07-30 Dsm Ip Assets Bv Factores para la producción y acumulación de ácidos grasos poliinsaturados (PUFA) obtenidos con PUFA sintasas
EP3268457A4 (en) * 2015-03-12 2018-10-24 Synthetic Genomics, Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
EP3302032B1 (en) 2015-06-06 2021-12-15 DSM IP Assets B.V. Production of polyunsaturated fatty acids (pufas) using a novel modular docosahexaenoic acid (dha) synthase
EP3455351B1 (en) 2016-05-12 2022-08-24 DSM IP Assets B.V. Method of increasing omega-3 polyunsaturated fatty acids production in microalgae
WO2018013821A1 (en) 2016-07-13 2018-01-18 Dsm Ip Assets B.V. A crispr-cas system for an algal host cell
WO2018205165A1 (zh) * 2017-05-10 2018-11-15 南京工业大学 一株裂殖壶菌菌株、其构建方法及应用
EP3628679B1 (en) * 2017-05-31 2024-07-17 Xiamen Huison Biotech Co., Ltd. Bacterium producing dha and epa, 6 gene fragments of genome of bacterium and use thereof
CN107384806A (zh) * 2017-08-28 2017-11-24 厦门大学 一种基于同源重组的裂殖壶菌基因表达系统的构建及应用
WO2020032261A1 (ja) 2018-08-10 2020-02-13 協和発酵バイオ株式会社 エイコサペンタエン酸を生産する微生物及びエイコサペンタエン酸の製造法
WO2023144707A1 (en) 2022-01-25 2023-08-03 Dsm Ip Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production
WO2025238190A1 (en) 2024-05-15 2025-11-20 Dsm Ip Assets B.V. Spray-dried powders having high pufa content

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0290799B9 (en) 1983-01-13 2004-09-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Transgenic dicotyledonous plant cells and plants
US6051757A (en) 1983-01-14 2000-04-18 Washington University Regeneration of plants containing genetically engineered T-DNA
JPS60500438A (ja) 1983-01-17 1985-04-04 モンサント カンパニ− 植物細胞を形質転換するためのプラスミド
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8317248D0 (en) 1983-06-24 1983-07-27 Wyeth John & Brother Ltd Fat compositions
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
AU7360087A (en) 1986-04-30 1987-11-24 Boyce Thompson Institute For Plant Research Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5246841A (en) * 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
EP0292435B1 (en) 1987-05-20 1995-07-26 Ciba-Geigy Ag Zea mays plants and transgenic zea mays plants regenerated from protoplasts or protoplast-derived cells
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
JP3234598B2 (ja) 1990-11-23 2001-12-04 プラント・ジエネテイツク・システムズ・エヌ・ベー 単子葉植物の形質転換方法
US5601860A (en) 1990-11-30 1997-02-11 American Home Products Corporation Corandomized fat compositions for infant formulas
US5141141A (en) 1990-12-21 1992-08-25 Nanette Leone Bag for use on the body
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5639790A (en) * 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
CA2124673C (en) 1991-12-04 2008-08-05 John Browse Fatty acid desaturase genes from plants
JPH07505531A (ja) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ 単子葉植物細胞の形質転換法
US5798259A (en) * 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
EP0594868A4 (en) 1992-05-15 1997-01-02 Sagami Chem Res Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
US5683898A (en) * 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
DK0604662T3 (da) 1992-07-07 2008-10-20 Japan Tobacco Inc Fremgangsmåde til transformering af monocotyledon
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
EP0627752B1 (en) 1993-06-04 1997-07-23 CAVIS S.r.l. An inertia device for interrupting the electrical circuit of a vehicle with an internal combustion engine
DE4323727A1 (de) * 1993-07-15 1995-03-09 Boehringer Mannheim Gmbh Verfahren zur Identifizierung von menschlichen und tierischen Zellen mit der Fähigkeit zu unbegrenzter Proliferation oder zur Tumorbildung
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
EP0831149A4 (en) 1995-01-13 2000-12-06 Sagami Chem Res GENES CODING FOR THE GROUP OF ENZYMES FOR BIOSYNTHESIS OF EICOSAPENTIC ACID AND METHOD FOR PRODUCING EICOSAPENTIC ACID
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
WO1996033263A1 (en) 1995-04-17 1996-10-24 JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
WO1997013402A1 (en) 1995-10-13 1997-04-17 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
AR006830A1 (es) 1996-04-26 1999-09-29 Du Pont Aceite de soja con alta estabilidad oxidativa
EP0913473A4 (en) 1996-07-10 2000-12-06 Sagami Chem Res METHOD FOR PRODUCING EICOSAPENTIC ACID BY GENETIC RECOMBINATION
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
CN1191828C (zh) 1997-03-27 2005-03-09 布里斯托尔-迈尔斯斯奎布公司 二十二碳六烯酸和花生四烯酸促进不足月婴儿生长的用途
SA98190002B1 (ar) 1997-03-28 2006-06-20 ذي يونيفيرسيتي اوف تننيسي ريسيرش كوربوريشن استخدام أحماض دهنية تحتوي على عدة روابط غير مشبعة polyunsaturated fatty acids لتقليل حدوث الالتهاب المعوي القولوني الناخر incidence of necrotizing enterocolitis
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
EP1598422B1 (en) 1997-04-11 2013-01-02 Calgene LLC Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
US6051754A (en) 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
CA2283422A1 (en) * 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US6013665A (en) 1997-12-16 2000-01-11 Abbott Laboratories Method for enhancing the absorption and transport of lipid soluble compounds using structured glycerides
US6677145B2 (en) * 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) * 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
AU2001268296B2 (en) * 2000-06-08 2006-05-25 Miami University Fatty acid elongase 3-ketoacyl coa synthase polypeptides
US20040010817A1 (en) * 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
DK1911837T3 (da) * 2000-09-28 2011-08-29 Bioriginal Food & Science Corp FAD5-2 fedtsyredesaturasefamiliemedlem og anvendelser deraf
TWI426127B (zh) 2001-04-16 2014-02-11 Dsm智慧財產有限公司 多不飽和脂肪酸(pufa)聚乙醯合成酶系統及其用途(三)
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
CA2387401C (en) 2001-05-21 2004-10-12 Ecopia Biosciences Inc. Compositions, methods and systems for the production of enediynes
US20040005672A1 (en) * 2002-02-22 2004-01-08 Santi Daniel V. Heterologous production of polyketides
GB2385852A (en) * 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US7705202B2 (en) * 2002-03-16 2010-04-27 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
KR101234198B1 (ko) 2003-03-26 2013-02-19 마텍 바이오싸이언스스 코포레이션 Pufa 폴리케타이드 신타제 시스템 및 이의 용도
AU2004225838B2 (en) 2003-03-31 2009-11-05 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
EP1720539A4 (en) 2004-02-13 2010-07-21 Martek Biosciences Corp SCHIZOCHYTRIUM FATTY ACID SYNTHASE (FAS) AND RELEVANT PRODUCTS AND METHOD
DE102004017370A1 (de) * 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Gene aus Ulkenia
DE102004017369A1 (de) * 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screeningverfahren zur Identifizierung von PUFA-PKS in Proben
US20050255440A1 (en) * 2004-05-12 2005-11-17 Downing Linda P System and method of integrating levels of educational programs
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
AR050881A1 (es) 2004-09-20 2006-11-29 Basf Plant Science Gmbh Genes de arabidopsis que codifican proteinas que participan en el metabolismo de azucares y lipidos y metodos de uso
US7355100B2 (en) 2004-09-30 2008-04-08 Dow Agrosciences Llc Canola cultivar NQC02CNX12
US7348473B2 (en) 2004-09-30 2008-03-25 Dow Agrosciences Llc Canola cultivar NQC02CNX25
US7456340B2 (en) 2005-04-04 2008-11-25 Dow Agrosciences Llc Canola cultivar NQC02CNX21
GB0421937D0 (en) 2004-10-02 2004-11-03 Univ York Acyl CoA synthetases
CA2613294A1 (en) 2005-05-17 2006-11-23 Photon Therapy Systems (Proprietary) Limited A photon therapy device
WO2006135866A2 (en) 2005-06-10 2006-12-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
WO2007017952A1 (ja) 2005-08-11 2007-02-15 Fujitsu Limited 光学素子および光情報記録再生装置
WO2007106903A2 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
JP2009530622A (ja) 2006-03-15 2009-08-27 クゥアルコム・インコーポレイテッド 全地球的航法衛星システム
AU2013251201A1 (en) 2006-03-15 2013-11-21 Dsm Ip Assets B.V. Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
ES2717102T3 (es) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
CA2993690C (en) * 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
TW201525136A (zh) * 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌PUFA合成酶於油籽作物中生成ω-3長鏈多不飽和脂肪酸

Also Published As

Publication number Publication date
US20170088841A1 (en) 2017-03-30
CA2755639A1 (en) 2010-09-23
ES2623610T3 (es) 2017-07-11
US8940884B2 (en) 2015-01-27
CN102741267A (zh) 2012-10-17
EP2408797A2 (en) 2012-01-25
CA3012998A1 (en) 2010-09-23
AU2010226440B2 (en) 2015-08-20
EP3192871B1 (en) 2019-01-23
TWI600762B (zh) 2017-10-01
CA3012998C (en) 2021-09-07
EP2408797B1 (en) 2017-03-15
JP2015231384A (ja) 2015-12-24
AU2010226440A1 (en) 2011-11-03
AR075897A1 (es) 2011-05-04
CN102741267B (zh) 2020-06-23
JP6432867B2 (ja) 2018-12-05
US12203075B2 (en) 2025-01-21
BRPI1006435B1 (pt) 2021-01-19
US20200199598A1 (en) 2020-06-25
JP6659044B2 (ja) 2020-03-04
US20150152450A1 (en) 2015-06-04
TW201038734A (en) 2010-11-01
EP2408797A4 (en) 2013-01-09
ES2717102T3 (es) 2019-06-19
EP3192871A1 (en) 2017-07-19
BRPI1006435A2 (pt) 2016-10-04
JP2017213008A (ja) 2017-12-07
WO2010108114A3 (en) 2010-12-23
US9540666B2 (en) 2017-01-10
US10538772B2 (en) 2020-01-21
JP2012521195A (ja) 2012-09-13
US20100266564A1 (en) 2010-10-21
CA2755639C (en) 2018-09-25
WO2010108114A2 (en) 2010-09-23

Similar Documents

Publication Publication Date Title
BR112014001907A2 (pt) produção de dha e outros lc-pufas em plantas
WO2010108114A3 (en) Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
AR084377A1 (es) Metodos y composiciones relacionados con enzimas de alcohol graso biosintetico
MX2015003305A (es) Variantes de enzima con propiedades de ester sintasa mejoradas.
EA201590026A1 (ru) Выработка длинноцепочечных полиненасыщенных жирных кислот в растительных клетках
BR112014007115A2 (pt) éster sintases de cera acil-acp
EA201270151A1 (ru) Способы и композиции для рекомбинантного биосинтеза h-алканов
WO2014093505A8 (en) Acp-mediated production of fatty acid derivatives
CO6351813A2 (es) "metodos y composiciones para mejorar la produccion de productos en microorganismos"
WO2007136428A8 (en) Nutraceutical compositions from microalgae and related methods of production and administration
MX385408B (es) Microorganismos que producen ácido eicosapentaenoico, composiciones de ácidos grasos, y métodos para prepararlos y usos de los mismos.
BRPI0809345A2 (pt) Células recombinantes, composições produzidas por estas, métodos para produzir derivados de ácidos graxos em uma célula recombinante, para aumentar a produção de derivados de ácidos graxos em uma célula recombinante, construto recombinante, vetores, métodos para aumentar a produção de derivados de ácidos graxos em uma célula hospedeira de produção, para produzir derivados de ácidos graxos, derivados de ácidos graxos, composições de biocombustível.
MY165098A (en) Production of dha and other lc-pufas in plants
MX2011011842A (es) Composiciones, metodos y equipos para acidos grasos poliinsaturados a partir de microalgas.
AR098891A1 (es) Proceso para obtener aceite microbiano a partir de células microbianas
BR112012020261A2 (pt) micro-organismos de levedura com acumulação de subproduto reduzida para produção melhorada de combustíveis, produtos químicos e aminoácidos.
BR112015009237A2 (pt) Composição compreendendo beta-glicosidase de magnaporthe grisea, ácido nucleico, célula hospedeira e métodos para produzir beta-glicosidase e para hidrolisar substrato de biomassa lignocelulósica
BR112014001174A2 (pt) métodos para aumento de rendimentos de produto
BR112016029391A2 (pt) métodos, reagentes e células para biossintetizar compostos
BR112015007329A2 (pt) organismos recombinantes
BR112012015119A2 (pt) "ácidos graxos de cadeia ramificada e produção biológica dos mesmos".
BR112014032741A2 (pt) lipase em esterificação de ácidos graxos de cadeia curta
GB201122220D0 (en) Novel emoillients
EP4282972A3 (en) Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
BR112016001918A2 (pt) Semente de soja transgênica, farelo, progênie, produtos e subprodutos, métodos para aumentar ácidos graxos totais e a percentagem de ácidos graxos totais, terceira planta transgênica e alimento ou ração

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: DSM IP ASSETS B.V. (NL)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 19/01/2021, OBSERVADAS AS CONDICOES LEGAIS.