BRPI1008965A2 - aumento de escala de dispositivos microfluídicos - Google Patents

aumento de escala de dispositivos microfluídicos

Info

Publication number
BRPI1008965A2
BRPI1008965A2 BRPI1008965A BRPI1008965A BRPI1008965A2 BR PI1008965 A2 BRPI1008965 A2 BR PI1008965A2 BR PI1008965 A BRPI1008965 A BR PI1008965A BR PI1008965 A BRPI1008965 A BR PI1008965A BR PI1008965 A2 BRPI1008965 A2 BR PI1008965A2
Authority
BR
Brazil
Prior art keywords
scaling
microfluidic devices
microfluidic
devices
Prior art date
Application number
BRPI1008965A
Other languages
English (en)
Inventor
Adam R Abate
David A Weitz
Mark Romanowsky
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of BRPI1008965A2 publication Critical patent/BRPI1008965A2/pt
Publication of BRPI1008965B1 publication Critical patent/BRPI1008965B1/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B01F23/4105—Methods of emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00002—Chemical plants
    • B01J2219/00004—Scale aspects
    • B01J2219/00015—Scale-up
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00824—Ceramic
    • B01J2219/00828—Silicon wafers or plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00831—Glass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00833—Plastic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00819—Materials of construction
    • B01J2219/00837—Materials of construction comprising coatings other than catalytically active coatings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00889—Mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00891—Feeding or evacuation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00952—Sensing operations
    • B01J2219/00968—Type of sensors
    • B01J2219/0097—Optical sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/0095—Control aspects
    • B01J2219/00952—Sensing operations
    • B01J2219/00968—Type of sensors
    • B01J2219/0097—Optical sensors
    • B01J2219/00975—Ultraviolet light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)
BRPI1008965A 2009-03-13 2010-03-12 método para aumento de escala de dispositivos microfluídicos e sistema para a formação de gotículas em canais microfluídicos em paralelo BRPI1008965B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16018409P 2009-03-13 2009-03-13
US22362709P 2009-07-07 2009-07-07
PCT/US2010/000753 WO2010104597A2 (en) 2009-03-13 2010-03-12 Scale-up of microfluidic devices

Publications (2)

Publication Number Publication Date
BRPI1008965A2 true BRPI1008965A2 (pt) 2016-03-15
BRPI1008965B1 BRPI1008965B1 (pt) 2018-12-18

Family

ID=42728998

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1008965A BRPI1008965B1 (pt) 2009-03-13 2010-03-12 método para aumento de escala de dispositivos microfluídicos e sistema para a formação de gotículas em canais microfluídicos em paralelo

Country Status (7)

Country Link
US (6) US9056299B2 (pt)
EP (1) EP2406003A2 (pt)
JP (2) JP5909095B2 (pt)
KR (1) KR101793744B1 (pt)
CN (1) CN102405098A (pt)
BR (1) BRPI1008965B1 (pt)
WO (1) WO2010104597A2 (pt)

Families Citing this family (128)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1886315B (zh) 2003-10-30 2012-11-28 塞通诺米/St有限责任公司 多层流体动力学鞘流结构
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
US9289765B2 (en) * 2008-09-23 2016-03-22 Samsung Electronics Co., Ltd. Micro-fluidic device and sample testing apparatus using the same
US9056299B2 (en) 2009-03-13 2015-06-16 President And Fellows Of Harvard College Scale-up of flow-focusing microfluidic devices
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
US20120167410A1 (en) 2010-12-21 2012-07-05 Basf Se Spray drying techniques
EP2714254B1 (en) * 2011-05-23 2017-09-06 President and Fellows of Harvard College Control of emulsions, including multiple emulsions
EP2535105A1 (en) 2011-06-14 2012-12-19 Corning Incorporated Systems and methods for scale-up of microreactors
CN103764265A (zh) 2011-07-06 2014-04-30 哈佛学院院长等 多重乳剂和用于配制多重乳剂的技术
PL221042B1 (pl) * 2011-07-27 2016-02-29 Inst Chemii Fizycznej Polskiej Akademii Nauk Urządzenie i sposób do wysokoprzepustowego tworzenia i łączenia kropli na żądanie
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
WO2014047236A2 (en) 2012-09-21 2014-03-27 President And Fellows Of Harvard College Systems and methods for spray drying in microfluidic and other systems
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2954065T3 (da) 2013-02-08 2021-09-06 10X Genomics Inc Opdeling og bearbejdning af analytter og andre arter
EP2972212B1 (en) 2013-03-14 2022-12-21 Cytonome/ST, LLC Hydrodynamic focusing apparatus and methods
CN105339086A (zh) * 2013-05-31 2016-02-17 星阵私人有限公司 一种乳液中的材料进入装置部件的孔中的处理方法
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US20160271609A1 (en) 2013-10-03 2016-09-22 The Trustees Of The University Of Pennsylvania Highly parallelized droplet microfluidic apparatus
US10159979B2 (en) 2013-11-11 2018-12-25 King Abdullah University Of Science And Technology Microfluidic device for high-volume production of monodisperse emulsions
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US20170173578A1 (en) * 2014-03-14 2017-06-22 Board Of Regents, The University Of Texas System Microfluidic devices for the rapid detection of analytes
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
WO2015160919A1 (en) 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
KR102531677B1 (ko) 2014-06-26 2023-05-10 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
CN106575322B (zh) 2014-06-26 2019-06-18 10X基因组学有限公司 核酸序列装配的方法和系统
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
US11117068B2 (en) * 2014-10-20 2021-09-14 Numerical Designs, Inc. Microfluidic-based apparatus and method for vaporization of liquids
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
WO2016085743A1 (en) 2014-11-24 2016-06-02 President And Fellows Of Harvard College Methods and systems for encapsulation of actives within droplets and other compartments
KR102321863B1 (ko) 2015-01-12 2021-11-08 10엑스 제노믹스, 인크. 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리
CN107209814B (zh) 2015-01-13 2021-10-15 10X基因组学有限公司 用于使结构变异和相位信息可视化的系统和方法
EP3256606B1 (en) 2015-02-09 2019-05-22 10X Genomics, Inc. Systems and methods for determining structural variation
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
WO2016138175A1 (en) * 2015-02-24 2016-09-01 The University Of British Columbia Continuous flow microfluidic system
WO2016164738A1 (en) 2015-04-08 2016-10-13 Board Of Regents, The University Of Texas System Methods and systems for the detection of analytes
CN107921381B (zh) 2015-04-28 2021-11-09 不列颠哥伦比亚大学 一次性微流控盒
US20180155957A1 (en) * 2015-05-19 2018-06-07 Mul-T-Lock Technologies Ltd. Method for waterproofing a lock device
GB201510189D0 (en) * 2015-06-11 2015-07-29 Univ Edinburgh Microfluidic device
US10035887B2 (en) * 2015-08-19 2018-07-31 Shimadzu Corporation Manufacturing method for nanoparticle
US10799867B2 (en) * 2015-09-24 2020-10-13 The Trustees Of The University Of Pennsylvania Apparatus for generating microdroplets and methods of manufacturing
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
WO2017106252A1 (en) * 2015-12-16 2017-06-22 The Trustees Of The University Of Pennsylvania Large scale microdroplet generation apparatus and methods of manufacturing thereof
CN108778477B (zh) 2016-01-06 2022-02-25 不列颠哥伦比亚大学 分叉混合器及其使用和制造方法
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN106492716B (zh) * 2016-12-20 2024-01-30 中国工程物理研究院激光聚变研究中心 一体式双重乳粒发生装置及其加工方法
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
EP3625715A4 (en) 2017-05-19 2021-03-17 10X Genomics, Inc. DATA SET ANALYSIS SYSTEMS AND METHODS
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US20210187508A1 (en) 2017-10-16 2021-06-24 1 CellBio ,Inc. Systems and methods for particulate encapsulation in microdroplets
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP4241882B1 (en) 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
ES2983417T3 (es) 2018-01-29 2024-10-23 St Jude Childrens Res Hospital Inc Método para la amplificación de ácidos nucleicos
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US10731012B2 (en) 2018-11-06 2020-08-04 President And Fellows Of Harvard College Anti-clogging microfluidic multichannel device
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN109622080B (zh) * 2019-01-08 2021-04-06 新羿制造科技（北京）有限公司 用于多指标分析的微液滴生成芯片
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
CN109799528B (zh) * 2019-01-17 2022-08-19 中国辐射防护研究院 用于放射性污染金属浅层取样的氧化凝胶及其制备方法
WO2020160152A1 (en) * 2019-01-31 2020-08-06 The Trustees Of The University Of Pennsylvania Silicon chip having multi-zone through silicon vias and method of manufacturing the same
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
WO2021025131A1 (ja) * 2019-08-08 2021-02-11 パナソニックＩｐマネジメント株式会社 マイクロ流体装置
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
GB2604481A (en) 2019-10-10 2022-09-07 1859 Inc Methods and systems for microfluidic screening
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021163630A1 (en) 2020-02-13 2021-08-19 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN112275336B (zh) * 2020-10-20 2021-11-19 大连理工大学 一种多通道集成微流控芯片及其高通量制备单分散凝胶微球的方法
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
CN112255051B (zh) * 2020-11-17 2022-07-08 攀钢集团攀枝花钢铁研究院有限公司 一种开放式管道的矿浆取样装置
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN113304790A (zh) * 2021-05-26 2021-08-27 南京工业大学 以并行化设计实现微液滴高通量制备的三维微流控芯片

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US458398A (en) 1891-08-25 Machine
US2422804A (en) 1946-01-26 1947-06-24 Walter H Schroeder Kite
US4044210A (en) 1975-07-17 1977-08-23 Westinghouse Electric Corporation Fluid-blast circuit interrupter
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
KR100314996B1 (ko) 1994-11-10 2002-01-15 윌리암 제이. 버크 액체분배시스템
WO1996029629A2 (en) 1995-03-01 1996-09-26 President And Fellows Of Harvard College Microcontact printing on surfaces and derivative articles
CZ392697A3 (cs) 1995-06-07 1998-06-17 Lynx Therapeutics, Inc. Oligonukleotidové značky pro stanovení druhu a identifikaci
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
ES2283936T3 (es) 1997-07-07 2007-11-01 Medical Research Council Procedimiento de clasificacion in vitro.
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6368562B1 (en) 1999-04-16 2002-04-09 Orchid Biosciences, Inc. Liquid transportation system for microfluidic device
US6268219B1 (en) 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
EP1294504B1 (en) 2000-06-30 2004-09-29 Ariel Industries Plc Controlled flow of displaced material in self-pierce fastening
EP1334347A1 (en) 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods
US6827095B2 (en) 2000-10-12 2004-12-07 Nanostream, Inc. Modular microfluidic systems
CA2438856C (en) 2001-02-23 2007-08-07 Japan Science And Technology Corporation Process and apparatus for producing emulsion and microcapsules
US6981522B2 (en) 2001-06-07 2006-01-03 Nanostream, Inc. Microfluidic devices with distributing inputs
US7141429B2 (en) * 2001-10-09 2006-11-28 University Of Washington Use of liquid junction potentials for electrophoresis without applied voltage in a microfluidic channel
GB0126281D0 (en) * 2001-11-01 2002-01-02 Astrazeneca Ab A chemical reactor
US6923567B2 (en) 2002-04-12 2005-08-02 Hynetics Llc Mixing tank assembly
DE60333995D1 (de) * 2002-05-09 2010-10-14 Univ Chicago Einrichtugn und verfahren für druckgesteuerten plug-transport und reaktion
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
AU2004209001B2 (en) 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20100022414A1 (en) * 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
EP2127736A1 (en) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Formation and control of fluidic species
TW577855B (en) * 2003-05-21 2004-03-01 Univ Nat Cheng Kung Chip-type micro-fluid particle 3-D focusing and detection device
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
BRPI0414004A (pt) 2003-08-27 2006-10-24 Harvard College controle eletrÈnico de espécies fluìdicas
CN1886315B (zh) * 2003-10-30 2012-11-28 塞通诺米/St有限责任公司 多层流体动力学鞘流结构
US7459508B2 (en) 2004-03-02 2008-12-02 Velocys, Inc. Microchannel polymerization reactor
JP4639624B2 (ja) 2004-03-30 2011-02-23 東ソー株式会社 微小流路構造体
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006051552A2 (en) 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
JP4631508B2 (ja) * 2005-03-31 2011-02-16 Ｄｉｃ株式会社 マイクロミキサー
EP1931456B1 (en) * 2005-10-06 2010-06-30 Unilever PLC Microfluidic network and method
JP4639391B2 (ja) * 2005-10-06 2011-02-23 財団法人生産技術研究奨励会 微小液滴の溶合による液滴の形成方法及びその装置
US7709544B2 (en) * 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
US7932037B2 (en) 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
JP4713397B2 (ja) * 2006-01-18 2011-06-29 株式会社リコー 微小流路構造体及び微小液滴生成システム
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
AU2007210152A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
US20070246106A1 (en) * 2006-04-25 2007-10-25 Velocys Inc. Flow Distribution Channels To Control Flow in Process Channels
EP4190448A3 (en) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Microfluidic devices
EP2263789A1 (en) * 2006-05-11 2010-12-22 Corning Incorporated High throughput thermally tempered microreactor devices and methods
US9562837B2 (en) * 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8524173B2 (en) 2006-09-01 2013-09-03 Tosoh Corporation Microchannel structure and fine-particle production method using the same
JP5076742B2 (ja) 2006-09-01 2012-11-21 東ソー株式会社 微小流路構造体およびそれを用いた微小粒子製造方法
JP4449997B2 (ja) 2007-03-12 2010-04-14 株式会社日立製作所 マイクロリアクタシステム
EP2136786B8 (en) * 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
WO2008148200A1 (en) 2007-06-05 2008-12-11 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009011808A1 (en) 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
US20100173294A1 (en) * 2007-09-11 2010-07-08 Roche Molecular Systems, Inc. Diagnostic test for susceptibility to b-raf kinase inhibitors
CN102124259B (zh) * 2008-05-16 2015-12-16 哈佛大学 在包含微流体系统的流体系统中的阀和其它流动控制
WO2009153115A1 (en) 2008-06-20 2009-12-23 Unilever Plc Apparatus and method for emulsion manufacture
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9056299B2 (en) 2009-03-13 2015-06-16 President And Fellows Of Harvard College Scale-up of flow-focusing microfluidic devices
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
GB2498898B (en) 2010-11-05 2015-04-15 Lg Electronics Inc Method for performing handover in wireless communication system
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
CA2848304A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior University Methods for sequencing a polynucleotide
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
DK2954065T3 (da) 2013-02-08 2021-09-06 10X Genomics Inc Opdeling og bearbejdning af analytter og andre arter

Also Published As

Publication number Publication date
KR20120013320A (ko) 2012-02-14
US20170151536A1 (en) 2017-06-01
US10518230B2 (en) 2019-12-31
JP2012520174A (ja) 2012-09-06
BRPI1008965B1 (pt) 2018-12-18
JP5909095B2 (ja) 2016-04-26
US20230302420A1 (en) 2023-09-28
US20200306706A1 (en) 2020-10-01
US9486757B2 (en) 2016-11-08
CN102405098A (zh) 2012-04-04
WO2010104597A2 (en) 2010-09-16
US9056299B2 (en) 2015-06-16
US20250033009A1 (en) 2025-01-30
US20120121481A1 (en) 2012-05-17
JP2016005837A (ja) 2016-01-14
US11517864B2 (en) 2022-12-06
WO2010104597A3 (en) 2011-03-24
JP6388852B2 (ja) 2018-09-12
EP2406003A2 (en) 2012-01-18
US20150360186A1 (en) 2015-12-17
KR101793744B1 (ko) 2017-11-03

Similar Documents

Publication Publication Date Title
BRPI1008965A2 (pt) aumento de escala de dispositivos microfluídicos
EP2473857A4 (en) MICROFLUIDIC INTERFACE
EP2375112A4 (en) SEALING DEVICE
DK2506745T3 (da) Patron-ekstraktionsindretning
BR112012004536A2 (pt) dispositivo
IT1400767B1 (it) Microsistema
UA19989S (uk) Пристрій гіпертермомасажний
BR112012000937A2 (pt) dispositivo de conexão
BR112012005782A2 (pt) dispositivo de conexão
BR112012010974A2 (pt) dispositivo
PT3299800T (pt) Dispositivo microfluídico
BRPI1011991A2 (pt) dispositivo de perfuração
BR112012000284A2 (pt) dispositivo de contato
GB0910759D0 (en) Microfluidic device
BR112012002688A2 (pt) dispositivo de vedacao
PT2507635E (pt) Biomarcador sérico bioquímico
FR2947559B1 (fr) Fluides de laminage
GB0911572D0 (en) Microfluidic devices
DK2493768T3 (da) Etiketfjernelses-indretning
BR112012002883A2 (pt) dispositivo
FI20095852L (fi) Suuntaajalaite
BR112012007885A2 (pt) dispositivo de retensão
BR112012002889A2 (pt) dispositivo
BR112012009082A2 (pt) dispositivo de mão livres
EP2427631A4 (en) CARRIER LIQUIDS FOR AIR COVERAGE

Legal Events

Date Code Title Description
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/03/2010, OBSERVADAS AS CONDICOES LEGAIS.