BRPI1011000B8 - Método para a identificação por espectrometria de massa de micróbios que compreendem bactérias e leveduras no sangue - Google Patents

Método para a identificação por espectrometria de massa de micróbios que compreendem bactérias e leveduras no sangue

Info

Publication number
BRPI1011000B8
BRPI1011000B8 BRPI1011000A BRPI1011000A BRPI1011000B8 BR PI1011000 B8 BRPI1011000 B8 BR PI1011000B8 BR PI1011000 A BRPI1011000 A BR PI1011000A BR PI1011000 A BRPI1011000 A BR PI1011000A BR PI1011000 B8 BRPI1011000 B8 BR PI1011000B8
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BR
Brazil
Prior art keywords
mass spectrometry
blood
identification
pathogens
septicemia
Prior art date
Application number
BRPI1011000A
Other languages
English (en)
Inventor
Maier Thomas
Original Assignee
Bruker Daltonik Gmbh
Bruker Daltonics Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42782068&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI1011000(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bruker Daltonik Gmbh, Bruker Daltonics Gmbh & Co Kg filed Critical Bruker Daltonik Gmbh
Publication of BRPI1011000A2 publication Critical patent/BRPI1011000A2/pt
Publication of BRPI1011000B1 publication Critical patent/BRPI1011000B1/pt
Publication of BRPI1011000B8 publication Critical patent/BRPI1011000B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/26—Infectious diseases, e.g. generalised sepsis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Optics & Photonics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

DIAGNÓSTICO DA SEPTICEMIA POR ESPECTROMETRIA DE MASSA. A presente invenção refere-se principalmente à identificação de patógenos por espectrometria de massa em culturas de sangue provenientes de infecçoes na corrente sanguínea (septicemia). A invenção proporciona um método com o qual patógenos microbianos podem ser separados na forma purificada a partir de sangue após um cultivo relativamente breve num frasco de hemocultura, sem qualquer interferência de proteínas humanas ou frações residuais de partículas sanguíneas, como eritrócitos e leucócitos, e podem ser diretamente identificados por medição por espectrometria de massa de seus perfis proteicos. O método é baseado no uso de tensoativos relativamente fortes para destruir as partículas sanguíneas, dissolvendo as membranas celulares fracas e a maior parte das estruturas internas das partículas sanguíneas, apesar do fato dos tensoativos serem considerados fortes inibidores de ionização em MALDI e outros processos de ionização necessários para medições por espectrometria de massa. Esse método permite que patógenos desconhecidos sejam obtidos em sua forma pura por centrifugação ou filtração e sejam identificados no nível taxonômico de espécie ou subespécie. Problemas com DNA provenientes de altas concentrações de leucócitos podem ser resolvidos por meios especiais. Após o cultivo suficiente, a identificação em um laboratório de espectrometria de massa leva apenas meia hora.
BRPI1011000A 2009-07-16 2010-07-14 Método para a identificação por espectrometria de massa de micróbios que compreendem bactérias e leveduras no sangue BRPI1011000B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102009033368.1A DE102009033368B4 (de) 2009-07-16 2009-07-16 Massenspektrometrische Sepsisdiagnose
DE102009033368.1 2009-07-16
PCT/EP2010/060098 WO2011006911A2 (en) 2009-07-16 2010-07-14 Mass spectrometric diagnosis of septicemia

Publications (3)

Publication Number Publication Date
BRPI1011000A2 BRPI1011000A2 (pt) 2016-03-15
BRPI1011000B1 BRPI1011000B1 (pt) 2019-10-22
BRPI1011000B8 true BRPI1011000B8 (pt) 2023-03-21

Family

ID=42782068

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1011000A BRPI1011000B8 (pt) 2009-07-16 2010-07-14 Método para a identificação por espectrometria de massa de micróbios que compreendem bactérias e leveduras no sangue

Country Status (9)

Country Link
US (3) US8569010B2 (pt)
EP (1) EP2454377B2 (pt)
JP (1) JP5460866B2 (pt)
CN (1) CN102471798B (pt)
AU (1) AU2010272586B2 (pt)
BR (1) BRPI1011000B8 (pt)
CA (1) CA2763420A1 (pt)
DE (1) DE102009033368B4 (pt)
WO (1) WO2011006911A2 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007058516B4 (de) 2007-11-23 2017-08-10 Bruker Daltonik Gmbh Identifizierung von Erregern in Körperflüssigkeiten
FR2942806B1 (fr) * 2009-03-03 2011-09-02 Assist Publ Hopitaux De Paris Procede d'identification de germes en milieu liquide
DE102009033368B4 (de) * 2009-07-16 2023-01-26 Bruker Daltonics GmbH & Co. KG Massenspektrometrische Sepsisdiagnose
DE102010006450B4 (de) 2010-02-01 2019-03-28 Bruker Daltonik Gmbh Gestufte Suche nach Mikrobenspektren in Referenzbibiliotheken
DE102010023452B4 (de) 2010-06-11 2012-11-08 Bruker Daltonik Gmbh Massenspektrometrische Messung von β-Lactamase-Resistenzen
DE102010033105B4 (de) † 2010-08-02 2016-05-04 Bruker Daltonik Gmbh Massenspektrometrische Sepsisdiagnose ohne Blutkultur
CA2808485C (en) 2010-08-19 2019-02-26 Erasmus University Medical Center Rotterdam Methods and means for characterizing antibiotic resistance in microorganisms
CN103765216B (zh) * 2011-04-21 2017-08-11 生物梅里埃公司 使用质谱检测至少一种碳青霉烯抗性机制的方法
US9631221B1 (en) 2011-09-02 2017-04-25 Becton, Dickinson And Company Identification of microorganisms using MALDI-TOF-MS on-plate extraction
US9074236B2 (en) * 2012-05-01 2015-07-07 Oxoid Limited Apparatus and methods for microbial identification by mass spectrometry
DE102012011647B4 (de) * 2012-06-08 2020-07-02 Bruker Daltonik Gmbh Analyse von Mikroben aus Mikrokolonien mittels MALDI-Massenspektrometrie
FR3001464B1 (fr) * 2013-01-25 2016-02-26 Biomerieux Sa Procede d'isolement specifique d'acides nucleiques d'interet
US10041953B2 (en) 2013-04-04 2018-08-07 Erasmus University Medical Center Rotterdam Mass spectrometric determination of drug resistance
FI20165634L (fi) * 2016-08-25 2018-02-26 Thermo Fisher Scientific Oy Laite ja menetelmät mikro-organismien identifioimiseksi häiritsevästä matriisista
NL2019320B1 (en) * 2017-03-02 2018-09-21 Biosparq B V Diagnostic method and system for diagnosis
CN107629960A (zh) * 2017-07-21 2018-01-26 中国医学科学院北京协和医院 从阳性血分离细菌的试剂和方法及细菌鉴定方法
KR102638609B1 (ko) 2017-07-27 2024-02-19 바이오메리욱스, 인코포레이티드. 격리 튜브
JP7297273B2 (ja) * 2018-02-13 2023-06-26 株式会社島津製作所 微生物分析方法
JP2021093913A (ja) * 2018-03-28 2021-06-24 国立大学法人千葉大学 血液試料中の細菌の検出方法
WO2023285653A2 (en) 2021-07-15 2023-01-19 Universite Claude Bernard Lyon 1 Identification of microorganisms based on identification of peptides using a liquid separation device coupled with a mass spectrometer and processing means
EP4465012A4 (en) * 2022-01-13 2025-11-19 Hitachi High Tech Corp COLLECTION METHOD, TESTING METHOD, CONTAINER, CENTRIFUGE AND TESTING SYSTEM
CN114414341A (zh) * 2022-01-25 2022-04-29 厦门元谱生物科技有限公司 一种血液培养报阳的检测方法
JP2026013695A (ja) * 2024-07-17 2026-01-29 株式会社島津製作所 微生物の薬剤耐性評価方法
WO2026047783A1 (en) 2024-08-28 2026-03-05 Alifax S.R.L. Method for producing microbial pellets from biological samples

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DE102009033368B4 (de) * 2009-07-16 2023-01-26 Bruker Daltonics GmbH & Co. KG Massenspektrometrische Sepsisdiagnose

Also Published As

Publication number Publication date
WO2011006911A2 (en) 2011-01-20
JP5460866B2 (ja) 2014-04-02
US20140051117A1 (en) 2014-02-20
US20160130631A1 (en) 2016-05-12
DE102009033368B4 (de) 2023-01-26
CN102471798B (zh) 2014-08-20
DE102009033368A1 (de) 2011-01-27
AU2010272586B2 (en) 2013-12-05
US11473122B2 (en) 2022-10-18
US8569010B2 (en) 2013-10-29
JP2012532618A (ja) 2012-12-20
US20120115182A1 (en) 2012-05-10
CA2763420A1 (en) 2011-01-20
EP2454377B2 (en) 2021-05-26
BRPI1011000A2 (pt) 2016-03-15
EP2454377A2 (en) 2012-05-23
EP2454377B1 (en) 2013-05-29
CN102471798A (zh) 2012-05-23
AU2010272586A1 (en) 2012-02-09
WO2011006911A3 (en) 2011-04-14
BRPI1011000B1 (pt) 2019-10-22

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 14/07/2010, OBSERVADAS AS CONDICOES LEGAIS.

B25A Requested transfer of rights approved

Owner name: BRUKER DALTONICS GMBH AND CO. KG (DE)