CA1037366A - Methode de coloration des tissus - Google Patents

Methode de coloration des tissus

Info

Publication number: CA1037366A
Authority: CA; Canada
Prior art keywords: tissue; solution; hematoxylin; formaldehyde; zinc chloride
Prior art date: 1973-06-25
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA203,034A

Other languages

English (en)

Other versions

CA203034S (en

Inventor

James S. Harrison

Paul T. Wertlake

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Applied Bioscience

Original Assignee

Applied Bioscience

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-06-25

Filing date

1974-06-21

Publication date

1978-08-29

1974-06-21 Application filed by Applied Bioscience filed Critical Applied Bioscience

1978-08-29 Application granted granted Critical

1978-08-29 Publication of CA1037366A publication Critical patent/CA1037366A/fr

Status Expired legal-status Critical Current

Links

238000007447 staining method Methods 0.000 title abstract 3
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 38
WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims abstract description 26
JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims abstract description 19
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims abstract description 12
229960004319 trichloroacetic acid Drugs 0.000 claims abstract description 12
235000005074 zinc chloride Nutrition 0.000 claims abstract description 9
239000011592 zinc chloride Substances 0.000 claims abstract description 9
239000000243 solution Substances 0.000 claims description 25
238000000034 method Methods 0.000 claims description 20
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
238000007654 immersion Methods 0.000 claims description 8
XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 claims description 4
229910052808 lithium carbonate Inorganic materials 0.000 claims description 4
239000000203 mixture Substances 0.000 claims description 4
238000010186 staining Methods 0.000 claims description 4
AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 claims description 3
YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims description 3
239000007864 aqueous solution Substances 0.000 claims description 2
229940037003 alum Drugs 0.000 claims 1
230000001476 alcoholic effect Effects 0.000 abstract 1
210000001519 tissue Anatomy 0.000 description 19
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
238000002360 preparation method Methods 0.000 description 7
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
150000002500 ions Chemical class 0.000 description 5
CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 4
239000002253 acid Substances 0.000 description 4
210000004027 cell Anatomy 0.000 description 4
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 3
238000003745 diagnosis Methods 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
238000007598 dipping method Methods 0.000 description 2
235000011187 glycerol Nutrition 0.000 description 2
UKWHYYKOEPRTIC-UHFFFAOYSA-N mercury(ii) oxide Chemical compound [Hg]=O UKWHYYKOEPRTIC-UHFFFAOYSA-N 0.000 description 2
238000012545 processing Methods 0.000 description 2
BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
238000001356 surgical procedure Methods 0.000 description 2
HZLHRDBTVSZCBS-GHTYLULLSA-N 4-[(z)-(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline;hydrochloride Chemical compound Cl.C1=CC(=N)C(C)=C\C1=C(C=1C=C(C)C(N)=CC=1)\C1=CC=C(N)C=C1 HZLHRDBTVSZCBS-GHTYLULLSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
229960000583 acetic acid Drugs 0.000 description 1
150000001298 alcohols Chemical class 0.000 description 1
DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
210000003855 cell nucleus Anatomy 0.000 description 1
238000012790 confirmation Methods 0.000 description 1
239000013078 crystal Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
-1 e~hanol Chemical compound 0.000 description 1
239000000834 fixative Substances 0.000 description 1
239000012362 glacial acetic acid Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000007774 longterm Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000036210 malignancy Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
229940101209 mercuric oxide Drugs 0.000 description 1
238000007431 microscopic evaluation Methods 0.000 description 1
APVPOHHVBBYQAV-UHFFFAOYSA-N n-(4-aminophenyl)sulfonyloctadecanamide Chemical compound CCCCCCCCCCCCCCCCCC(=O)NS(=O)(=O)C1=CC=C(N)C=C1 APVPOHHVBBYQAV-UHFFFAOYSA-N 0.000 description 1
IPSIPYMEZZPCPY-UHFFFAOYSA-N new fuchsin Chemical compound [Cl-].C1=CC(=[NH2+])C(C)=CC1=C(C=1C=C(C)C(N)=CC=1)C1=CC=C(N)C(C)=C1 IPSIPYMEZZPCPY-UHFFFAOYSA-N 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
239000012188 paraffin wax Substances 0.000 description 1
AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 1
OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
238000004321 preservation Methods 0.000 description 1
238000011470 radical surgery Methods 0.000 description 1
NXLOLUFNDSBYTP-UHFFFAOYSA-N retene Chemical compound C1=CC=C2C3=CC=C(C(C)C)C=C3C=CC2=C1C NXLOLUFNDSBYTP-UHFFFAOYSA-N 0.000 description 1
230000000630 rising effect Effects 0.000 description 1
101150081985 scrib gene Proteins 0.000 description 1
239000012192 staining solution Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Immunology (AREA)
Chemical & Material Sciences (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Physics & Mathematics (AREA)
Physics & Mathematics (AREA)
Pathology (AREA)
Dentistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Environmental Sciences (AREA)
Investigating Or Analysing Biological Materials (AREA)
Sampling And Sample Adjustment (AREA)

CA203,034A 1973-06-25 1974-06-21 Methode de coloration des tissus Expired CA1037366A (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US37327673A	1973-06-25	1973-06-25

Publications (1)

Publication Number	Publication Date
CA1037366A true CA1037366A (fr)	1978-08-29

Family

ID=23471724

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA203,034A Expired CA1037366A (fr)	1973-06-25	1974-06-21	Methode de coloration des tissus

Country Status (16)

Country	Link
JP (1)	JPS5049026A (fr)
AU (1)	AU7049174A (fr)
BE (1)	BE816786R (fr)
BR (1)	BR7405213D0 (fr)
CA (1)	CA1037366A (fr)
DD (1)	DD112519A5 (fr)
DE (1)	DE2429647A1 (fr)
DK (1)	DK338574A (fr)
ES (1)	ES427568A1 (fr)
FI (1)	FI193074A7 (fr)
FR (1)	FR2234558B2 (fr)
HU (1)	HU168582B (fr)
NL (1)	NL7408556A (fr)
NO (1)	NO742274L (fr)
SE (1)	SE7407899L (fr)
ZA (1)	ZA743995B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH068818B2 (ja) *	1987-11-27	1994-02-02	サクラ精機株式会社	生体組織検査用固定液
EP1605244A1 (fr) *	2004-06-09	2005-12-14	Boon, Mathilde Elisabeth	Composition de fixation
CN110823666A (zh) *	2019-11-27	2020-02-21	李雄	苏木素在制备减少病理常规切片染色后褪色的染色剂中的应用

1974
- 1974-06-14 SE SE7407899A patent/SE7407899L/ not_active Application Discontinuation
- 1974-06-20 DE DE2429647A patent/DE2429647A1/de active Pending
- 1974-06-21 NO NO742274A patent/NO742274L/no unknown
- 1974-06-21 CA CA203,034A patent/CA1037366A/fr not_active Expired
- 1974-06-21 ZA ZA00743995A patent/ZA743995B/xx unknown
- 1974-06-22 ES ES427568A patent/ES427568A1/es not_active Expired
- 1974-06-24 DK DK338574A patent/DK338574A/da unknown
- 1974-06-24 BE BE145822A patent/BE816786R/fr active
- 1974-06-24 DD DD179409A patent/DD112519A5/xx unknown
- 1974-06-24 HU HUAI236A patent/HU168582B/hu unknown
- 1974-06-24 FI FI1930/74A patent/FI193074A7/fi unknown
- 1974-06-25 JP JP49072710A patent/JPS5049026A/ja active Pending
- 1974-06-25 NL NL7408556A patent/NL7408556A/xx unknown
- 1974-06-25 BR BR5213/74A patent/BR7405213D0/pt unknown
- 1974-06-25 AU AU70491/74A patent/AU7049174A/en not_active Expired
- 1974-06-25 FR FR7422137A patent/FR2234558B2/fr not_active Expired

Also Published As

Publication number	Publication date
NO742274L (fr)	1975-01-20
BE816786R (fr)	1974-12-24
HU168582B (fr)	1976-06-28
BR7405213D0 (pt)	1975-01-28
DE2429647A1 (de)	1975-01-16
ES427568A1 (es)	1976-07-16
SE7407899L (fr)	1974-12-27
DD112519A5 (fr)	1975-04-12
ZA743995B (en)	1975-06-25
FR2234558B2 (fr)	1977-02-11
AU7049174A (en)	1976-01-08
FI193074A7 (fr)	1974-12-26
JPS5049026A (fr)	1975-05-01
FR2234558A2 (fr)	1975-01-17
NL7408556A (fr)	1974-12-30
DK338574A (fr)	1975-02-24

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US3997656A (en)	1976-12-14	Tissue staining method and composition
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US3862300A (en)	1975-01-21	Histological fixative
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US5290706A (en)	1994-03-01	Visualization system for retrieval, identification, and positioning of biological samples for subsequent microscopic examination
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US4690901A (en)	1987-09-01	Silver methenamine staining method
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Scarano et al.	2003	Common fixatives in hard-tissue histology
Bentley et al.	1982	Histologic evaluation of horizontal frozen sections: improved staining and special staining techniques
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