CA1038195A - Separation des isoenzymes par echange d'ions - Google Patents

Separation des isoenzymes par echange d'ions

Info

Publication number: CA1038195A
Authority: CA; Canada
Prior art keywords: ion exchange; effluent; exchange agent; sample; molar
Prior art date: 1973-12-19
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA214,947A

Other languages

English (en)

Other versions

CA214947S (en

Inventor

Donald W. Mercer

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-12-19

Filing date

1974-11-29

Publication date

1978-09-12

1973-12-19 Priority claimed from US426022A external-priority patent/US3886898A/en

1974-11-29 Application filed by Individual filed Critical Individual

1978-09-12 Application granted granted Critical

1978-09-12 Publication of CA1038195A publication Critical patent/CA1038195A/fr

Status Expired legal-status Critical Current

Links

108010044467 Isoenzymes Proteins 0.000 title claims abstract description 70
238000000926 separation method Methods 0.000 title description 7
238000004255 ion exchange chromatography Methods 0.000 title description 2
102000004420 Creatine Kinase Human genes 0.000 claims abstract description 49
108010042126 Creatine kinase Proteins 0.000 claims abstract description 49
210000002966 serum Anatomy 0.000 claims abstract description 49
238000005342 ion exchange Methods 0.000 claims abstract description 44
238000000034 method Methods 0.000 claims abstract description 43
239000003795 chemical substances by application Substances 0.000 claims abstract description 40
102000003855 L-lactate dehydrogenase Human genes 0.000 claims abstract description 27
108700023483 L-lactate dehydrogenases Proteins 0.000 claims abstract description 27
239000007983 Tris buffer Substances 0.000 claims abstract description 14
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 14
239000000243 solution Substances 0.000 claims description 18
150000002500 ions Chemical class 0.000 claims description 16
239000000284 extract Substances 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 11
102000004190 Enzymes Human genes 0.000 claims description 7
108090000790 Enzymes Proteins 0.000 claims description 7
229920005654 Sephadex Polymers 0.000 claims description 3
239000012507 Sephadex™ Substances 0.000 claims description 3
102000013563 Acid Phosphatase Human genes 0.000 claims description 2
108010051457 Acid Phosphatase Proteins 0.000 claims description 2
229910001514 alkali metal chloride Inorganic materials 0.000 claims 11
150000001412 amines Chemical class 0.000 claims 3
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims 1
239000004382 Amylase Substances 0.000 claims 1
102000013142 Amylases Human genes 0.000 claims 1
102000004625 Aspartate Aminotransferases Human genes 0.000 claims 1
108010003415 Aspartate Aminotransferases Proteins 0.000 claims 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 claims 1
102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 claims 1
101710107035 Gamma-glutamyltranspeptidase Proteins 0.000 claims 1
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 1
101710173228 Glutathione hydrolase proenzyme Proteins 0.000 claims 1
239000003513 alkali Substances 0.000 claims 1
235000019418 amylase Nutrition 0.000 claims 1
102000006640 gamma-Glutamyltransferase Human genes 0.000 claims 1
229910001510 metal chloride Inorganic materials 0.000 claims 1
210000004357 third molar Anatomy 0.000 claims 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 24
208000010125 myocardial infarction Diseases 0.000 abstract description 21
239000011780 sodium chloride Substances 0.000 abstract description 12
239000007853 buffer solution Substances 0.000 abstract description 8
238000004587 chromatography analysis Methods 0.000 abstract description 5
201000010099 disease Diseases 0.000 abstract description 4
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
102000004169 proteins and genes Human genes 0.000 abstract description 4
108090000623 proteins and genes Proteins 0.000 abstract description 4
238000000502 dialysis Methods 0.000 abstract description 3
238000010828 elution Methods 0.000 abstract description 3
238000002798 spectrophotometry method Methods 0.000 abstract description 2
238000011156 evaluation Methods 0.000 abstract 1
230000000694 effects Effects 0.000 description 9
210000002381 plasma Anatomy 0.000 description 9
206010061216 Infarction Diseases 0.000 description 8
210000004556 brain Anatomy 0.000 description 6
230000007574 infarction Effects 0.000 description 6
210000001519 tissue Anatomy 0.000 description 6
210000002027 skeletal muscle Anatomy 0.000 description 5
208000029549 Muscle injury Diseases 0.000 description 4
210000005013 brain tissue Anatomy 0.000 description 4
239000003153 chemical reaction reagent Substances 0.000 description 4
238000003745 diagnosis Methods 0.000 description 4
210000002216 heart Anatomy 0.000 description 4
210000004369 blood Anatomy 0.000 description 3
239000008280 blood Substances 0.000 description 3
208000026106 cerebrovascular disease Diseases 0.000 description 3
238000010586 diagram Methods 0.000 description 3
208000006454 hepatitis Diseases 0.000 description 3
231100000283 hepatitis Toxicity 0.000 description 3
208000014674 injury Diseases 0.000 description 3
238000011084 recovery Methods 0.000 description 3
208000007848 Alcoholism Diseases 0.000 description 2
208000027418 Wounds and injury Diseases 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
150000001450 anions Chemical class 0.000 description 2
238000013194 cardioversion Methods 0.000 description 2
230000006378 damage Effects 0.000 description 2
238000010255 intramuscular injection Methods 0.000 description 2
239000000203 mixture Substances 0.000 description 2
210000003205 muscle Anatomy 0.000 description 2
230000002980 postoperative effect Effects 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
208000009304 Acute Kidney Injury Diseases 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
206010008479 Chest Pain Diseases 0.000 description 1
206010067125 Liver injury Diseases 0.000 description 1
208000019693 Lung disease Diseases 0.000 description 1
208000007101 Muscle Cramp Diseases 0.000 description 1
201000002481 Myositis Diseases 0.000 description 1
208000001647 Renal Insufficiency Diseases 0.000 description 1
208000033626 Renal failure acute Diseases 0.000 description 1
102000003929 Transaminases Human genes 0.000 description 1
108090000340 Transaminases Proteins 0.000 description 1
239000002253 acid Substances 0.000 description 1
201000011040 acute kidney failure Diseases 0.000 description 1
208000012998 acute renal failure Diseases 0.000 description 1
230000001476 alcoholic effect Effects 0.000 description 1
-1 anion ion Chemical class 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
230000003683 cardiac damage Effects 0.000 description 1
230000000747 cardiac effect Effects 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
230000001684 chronic effect Effects 0.000 description 1
239000013256 coordination polymer Substances 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000011491 glass wool Substances 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
210000005003 heart tissue Anatomy 0.000 description 1
231100000234 hepatic damage Toxicity 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007927 intramuscular injection Substances 0.000 description 1
201000006370 kidney failure Diseases 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
230000008818 liver damage Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
230000003387 muscular Effects 0.000 description 1
230000002107 myocardial effect Effects 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
230000002085 persistent effect Effects 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000000630 rising effect Effects 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
238000010186 staining Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
230000000451 tissue damage Effects 0.000 description 1
231100000827 tissue damage Toxicity 0.000 description 1
230000008733 trauma Effects 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

CA214,947A 1973-12-19 1974-11-29 Separation des isoenzymes par echange d'ions Expired CA1038195A (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US426022A US3886898A (en)	1973-12-19	1973-12-19	Multiple, contiguous stripe, extrusion coating apparatus
US47343874A	1974-05-28	1974-05-28

Publications (1)

Publication Number	Publication Date
CA1038195A true CA1038195A (fr)	1978-09-12

Family

ID=27026891

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA214,947A Expired CA1038195A (fr)	1973-12-19	1974-11-29	Separation des isoenzymes par echange d'ions

Country Status (1)

Country	Link
CA (1)	CA1038195A (fr)

1974
- 1974-11-29 CA CA214,947A patent/CA1038195A/fr not_active Expired

Publication	Publication Date	Title
Mercer	1974	Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography
Morelli et al.	1983	Serum creatine kinase MM isoenzyme sub-bands after acute myocardial infarction in man.
Varat et al.	1975	Cardiac specific creatine phosphokinase isoenzyme in the diagnosis of acute myocardial infarction.
Cohen et al.	1964	Serum lactic dehydrogenase isozyme patterns in cardiovascular and other diseases, with particular reference to acute myocardial infarction
Tsung et al.	1986	Creatine kinase isoenzymes in extracts of various human skeletal muscles.
Nealon et al.	1975	Separation of creatine kinase isoenzymes in serum by ion-exchange column chromatography (Mercer's method, modified to increase sensitivity)
Fiolet et al.	1977	Determination of creatine kinase isoenzyme MB (CK-MB): comparison of methods and clinical evaluation
Goldstein et al.	1972	Cystathionine synthase activity in human lymphocytes: induction by phytohemagglutinin
US4415655A (en)	1983-11-15	Electrophoretic separation of isoenzymes utilizing a stable polyacrylamide system
Quick et al.	1974	A kinetic study of the isozymes determined by the three human phosphoglucomutase loci PGM1, PGM2 and PGM3
JPS62224300A (ja)	1987-10-02	Ｄｎａが関連する病気にかかり易い体質または該病気に対する感受性を測定するための試験
US4046634A (en)	1977-09-06	Assay of isoenzymes by ion exchange chromatography
Green et al.	1972	Normal serum alkaline phosphatase isoenzymes examined by acrylamide and starch gel electrophoresis and by isoenzyme analysis using organ-specific inhibitors
Mercer	1975	Simultaneous separation of serum creatine kinase and lactate dehydrogenase isoenzymes by ion-exchange column chromatography
Shell et al.	1979	Sensitivity and specificity of MB creatine kinase activity determined with column chromatography
JP4602577B2 (ja)	2010-12-22	前糖尿病状態のスクリーニング方法及びスクリーニング用試薬
Klein et al.	1977	Separation of serum creatine kinase isoenzymes by ion-exchange column chromatography.
US4013513A (en)	1977-03-22	Ion exchange chromatographic isoenzyme separation and isolation
Gutensohn et al.	1972	Rapid assay for purine phosphoribosyltransferases
CA1320418C (fr)	1993-07-20	Reactif et methode de determination de l'isoenzyme ld-1
Al-Khalidi et al.	1970	A method for the determination of plasma guanase on finger-tip blood
DE3015462A1 (de)	1980-10-30	Verfahren zur bestimmung von interferon und reagens zur durchfuehrung des verfahrens
Kiessling et al.	1979	Detection of reverse transcriptase activity in human cells
Griffiths	1986	CK-MB: a valuable test?
FUJIMURA et al.	1982	Simultaneous quantitative estimation of galactose-1-phosphate and galactose in blood for the diagnosis of galactosemia