CA1060323A - Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule - Google Patents

Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule

Info

Publication number: CA1060323A
Authority: CA; Canada
Prior art keywords: lysate; buffer; endotoxin; absorbancy; aqueous
Prior art date: 1977-05-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA277,606A

Other languages

English (en)

Inventor

Kristine M. Kelly

Joseph D. Teller

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Worthington Biochemical Corp

Original Assignee

Worthington Biochemical Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1977-05-02

Filing date

1977-05-02

Publication date

1979-08-14

1977-05-02 Application filed by Worthington Biochemical Corp filed Critical Worthington Biochemical Corp

1977-05-02 Priority to CA277,606A priority Critical patent/CA1060323A/fr

1979-08-14 Application granted granted Critical

1979-08-14 Publication of CA1060323A publication Critical patent/CA1060323A/fr

Status Expired legal-status Critical Current

Links

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239000000872 buffer Substances 0.000 claims abstract description 48
239000007788 liquid Substances 0.000 claims abstract description 46
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238000011534 incubation Methods 0.000 claims abstract description 14
239000008280 blood Substances 0.000 claims abstract description 12
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238000006243 chemical reaction Methods 0.000 claims abstract description 7
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238000004108 freeze drying Methods 0.000 claims abstract description 6
239000000203 mixture Substances 0.000 claims description 30
238000000034 method Methods 0.000 claims description 25
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NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
235000021317 phosphate Nutrition 0.000 description 3
239000002904 solvent Substances 0.000 description 3
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GHAZCVNUKKZTLG-UHFFFAOYSA-N N-ethyl-succinimide Natural products CCN1C(=O)CCC1=O GHAZCVNUKKZTLG-UHFFFAOYSA-N 0.000 description 2
HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 description 2
GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
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OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
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ATADHKWKHYVBTJ-UHFFFAOYSA-N hydron;4-[1-hydroxy-2-(methylamino)ethyl]benzene-1,2-diol;chloride Chemical compound Cl.CNCC(O)C1=CC=C(O)C(O)=C1 ATADHKWKHYVBTJ-UHFFFAOYSA-N 0.000 description 1
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238000011046 pyrogen test Methods 0.000 description 1
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Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA277,606A 1977-05-02 1977-05-02 Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule Expired CA1060323A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA277,606A CA1060323A (fr)	1977-05-02	1977-05-02	Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA277,606A CA1060323A (fr)	1977-05-02	1977-05-02	Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule

Publications (1)

Publication Number	Publication Date
CA1060323A true CA1060323A (fr)	1979-08-14

Family

ID=4108570

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA277,606A Expired CA1060323A (fr)	1977-05-02	1977-05-02	Essais de turbidite pour la recherche de pyrogenes, effectues a l'aide de lyse de cellules de limule

Country Status (1)

Country	Link
CA (1)	CA1060323A (fr)

1977
- 1977-05-02 CA CA277,606A patent/CA1060323A/fr not_active Expired

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US4038029A (en)	1977-07-26	Limulus lysate turbidity test for pyrogens
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