CA1072634A - Analyse de glucose par polarographie - Google Patents

Analyse de glucose par polarographie

Info

Publication number: CA1072634A
Authority: CA; Canada
Prior art keywords: glucose; specimen; polarographic; electrodes; glucose oxidase
Prior art date: 1975-10-30
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA238,656A

Other languages

English (en)

Other versions

CA238656S (en

Inventor

Don N. Gray

Chung C. Young

Melvin H. Keyes

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer Corp

Original Assignee

Technicon Instruments Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1975-10-30

Filing date

1975-10-30

Publication date

1980-02-26

1975-10-30 Application filed by Technicon Instruments Corp filed Critical Technicon Instruments Corp

1975-10-30 Priority to CA238,656A priority Critical patent/CA1072634A/fr

1980-02-26 Application granted granted Critical

1980-02-26 Publication of CA1072634A publication Critical patent/CA1072634A/fr

Status Expired legal-status Critical Current

Links

WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 114
239000008103 glucose Substances 0.000 title claims abstract description 112
238000004458 analytical method Methods 0.000 title abstract description 21
239000004366 Glucose oxidase Substances 0.000 claims abstract description 80
108010015776 Glucose oxidase Proteins 0.000 claims abstract description 80
229940116332 glucose oxidase Drugs 0.000 claims abstract description 80
235000019420 glucose oxidase Nutrition 0.000 claims abstract description 80
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 62
238000000034 method Methods 0.000 claims abstract description 35
238000006243 chemical reaction Methods 0.000 claims description 19
239000003085 diluting agent Substances 0.000 claims description 19
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical group [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 9
239000011541 reaction mixture Substances 0.000 claims description 9
239000007795 chemical reaction product Substances 0.000 claims description 8
210000002966 serum Anatomy 0.000 claims description 7
230000004044 response Effects 0.000 claims description 6
229910021607 Silver chloride Inorganic materials 0.000 claims description 4
229910052697 platinum Inorganic materials 0.000 claims description 4
HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 4
239000007787 solid Substances 0.000 claims description 4
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical group [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
238000004891 communication Methods 0.000 claims description 2
238000007865 diluting Methods 0.000 claims description 2
229910052709 silver Inorganic materials 0.000 claims description 2
239000004332 silver Substances 0.000 claims description 2
RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 abstract description 14
238000007254 oxidation reaction Methods 0.000 abstract description 14
230000003647 oxidation Effects 0.000 abstract description 13
RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 abstract description 8
239000000174 gluconic acid Substances 0.000 abstract description 8
235000012208 gluconic acid Nutrition 0.000 abstract description 8
238000005259 measurement Methods 0.000 abstract description 5
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 102
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 33
210000004027 cell Anatomy 0.000 description 29
229960002163 hydrogen peroxide Drugs 0.000 description 25
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 23
239000012528 membrane Substances 0.000 description 20
239000000243 solution Substances 0.000 description 20
239000002131 composite material Substances 0.000 description 17
239000000203 mixture Substances 0.000 description 15
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 13
239000012153 distilled water Substances 0.000 description 13
239000001301 oxygen Substances 0.000 description 13
229910052760 oxygen Inorganic materials 0.000 description 13
108090000790 Enzymes Proteins 0.000 description 12
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QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 12
229940088598 enzyme Drugs 0.000 description 12
108090000854 Oxidoreductases Proteins 0.000 description 11
102000004316 Oxidoreductases Human genes 0.000 description 11
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
230000000694 effects Effects 0.000 description 8
239000011521 glass Substances 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 6
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
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AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 5
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 5
238000002347 injection Methods 0.000 description 5
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238000002156 mixing Methods 0.000 description 5
239000008363 phosphate buffer Substances 0.000 description 5
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
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ZNZJJSYHZBXQSM-UHFFFAOYSA-N propane-2,2-diamine Chemical compound CC(C)(N)N ZNZJJSYHZBXQSM-UHFFFAOYSA-N 0.000 description 3
239000011347 resin Substances 0.000 description 3
229920005989 resin Polymers 0.000 description 3
239000000377 silicon dioxide Substances 0.000 description 3
APQIUTYORBAGEZ-UHFFFAOYSA-N 1,1-dibromoethane Chemical compound CC(Br)Br APQIUTYORBAGEZ-UHFFFAOYSA-N 0.000 description 2
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
-1 Cyanogen Halide Chemical class 0.000 description 2
CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical class [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
238000001816 cooling Methods 0.000 description 2
239000003431 cross linking reagent Substances 0.000 description 2
239000008367 deionised water Substances 0.000 description 2
229910021641 deionized water Inorganic materials 0.000 description 2
238000001514 detection method Methods 0.000 description 2
238000010586 diagram Methods 0.000 description 2
238000009792 diffusion process Methods 0.000 description 2
238000010790 dilution Methods 0.000 description 2
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238000011049 filling Methods 0.000 description 2
239000012530 fluid Substances 0.000 description 2
230000005484 gravity Effects 0.000 description 2
239000001257 hydrogen Substances 0.000 description 2
229910052739 hydrogen Inorganic materials 0.000 description 2
XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 2
239000000463 material Substances 0.000 description 2
150000002978 peroxides Chemical class 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
231100000572 poisoning Toxicity 0.000 description 2
230000000607 poisoning effect Effects 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
239000012429 reaction media Substances 0.000 description 2
238000006479 redox reaction Methods 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
238000012360 testing method Methods 0.000 description 2
210000002700 urine Anatomy 0.000 description 2
238000005406 washing Methods 0.000 description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
BZWMYDJJDBFAPE-UHFFFAOYSA-N 2-chloro-4-phenylphenol Chemical compound C1=C(Cl)C(O)=CC=C1C1=CC=CC=C1 BZWMYDJJDBFAPE-UHFFFAOYSA-N 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 1
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
239000006087 Silane Coupling Agent Substances 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
206010042618 Surgical procedure repeated Diseases 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
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150000001450 anions Chemical class 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
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229940075397 calomel Drugs 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
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GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 1
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XFMJUIKWKVJNDY-UHFFFAOYSA-N diethoxyphosphorylsulfanylmethylbenzene Chemical compound CCOP(=O)(OCC)SCC1=CC=CC=C1 XFMJUIKWKVJNDY-UHFFFAOYSA-N 0.000 description 1
ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
239000012777 electrically insulating material Substances 0.000 description 1
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JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical class [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
239000010931 gold Chemical class 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
VKYKSIONXSXAKP-UHFFFAOYSA-N hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 description 1
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229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
239000004570 mortar (masonry) Substances 0.000 description 1
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PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
150000003057 platinum Chemical class 0.000 description 1
238000003969 polarography Methods 0.000 description 1
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102000004169 proteins and genes Human genes 0.000 description 1
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OGFYIDCVDSATDC-UHFFFAOYSA-N silver silver Chemical compound [Ag].[Ag] OGFYIDCVDSATDC-UHFFFAOYSA-N 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
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ZCUFMDLYAMJYST-UHFFFAOYSA-N thorium dioxide Chemical compound O=[Th]=O ZCUFMDLYAMJYST-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
229920003176 water-insoluble polymer Polymers 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

CA238,656A 1975-10-30 1975-10-30 Analyse de glucose par polarographie Expired CA1072634A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA238,656A CA1072634A (fr)	1975-10-30	1975-10-30	Analyse de glucose par polarographie

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA238,656A CA1072634A (fr)	1975-10-30	1975-10-30	Analyse de glucose par polarographie

Publications (1)

Publication Number	Publication Date
CA1072634A true CA1072634A (fr)	1980-02-26

Family

ID=4104400

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA238,656A Expired CA1072634A (fr)	1975-10-30	1975-10-30	Analyse de glucose par polarographie

Country Status (1)

Country	Link
CA (1)	CA1072634A (fr)

1975
- 1975-10-30 CA CA238,656A patent/CA1072634A/fr not_active Expired

Legal Events

Date	Code	Title	Description
1997-02-26	MKEX	Expiry

Publication	Publication Date	Title
US4517291A (en)	1985-05-14	Biological detection process using polymer-coated electrodes
Kanapieniene et al.	1992	Miniature glucose biosensor with extended linearity
Lowe	1984	Biosensors
US4655880A (en)	1987-04-07	Apparatus and method for sensing species, substances and substrates using oxidase
Miland et al.	1996	Poly (o-aminophenol)-modified bienzyme carbon paste electrode for the detection of uric acid
Harrison et al.	1988	Characterization of perfluorosulfonic acid polymer coated enzyme electrodes and a miniaturized integrated potentiostat for glucose analysis in whole blood
US4340448A (en)	1982-07-20	Potentiometric detection of hydrogen peroxide and apparatus therefor
US4356074A (en)	1982-10-26	Substrate specific galactose oxidase enzyme electrodes
CA1177735A (fr)	1984-11-13	Methode d'analyse polarographique de l'acide lactique et du lactate
Blaedel et al.	1980	Reagentless enzyme electrodes for ethanol, lactate, and malate
Karube et al.	1993	Microbiosensors for acetylcholine and glucose
Mascini et al.	1989	Glucose electrochemical probe with extended linearity for whole blood
Wang et al.	1990	A polishable amperometric biosensor for bilirubin
JPH09500727A (ja)	1997-01-21	電位差式バイオセンサー及びその使用方法
CA1039163A (fr)	1978-09-26	Analyse de l'uree
Papastathopoulos et al.	1975	Enzymic cholesterol determination using ion-selective membrane electrodes
Milardović et al.	1997	Glucose determination in blood samples using flow injection analysis and an amperometric biosensor based on glucose oxidase immobilized on hexacyanoferrate modified nickel electrode
Guilbault et al.	1973	Enzyme electrodes for the determination of 1-phenylalanine
Wang et al.	1991	One-step fabrication of glucose sensors based on entrapment of glucose oxidase within poly (ester-sulfonic acid) coatings
Wang et al.	1989	Flow cell based on glucose oxidase‐modified carbon fiber ultramicroelectrode
Llenado et al.	1973	Ion-electrode based automatic glucose analysis system
US4604182A (en)	1986-08-05	Perfluorosulfonic acid polymer-coated indicator electrodes
US5378332A (en)	1995-01-03	Amperometric flow injection analysis biosensor for glucose based on graphite paste modified with tetracyanoquinodimethane
US5306413A (en)	1994-04-26	Assay apparatus and assay method
Yao et al.	1995	On‐line amperometric assay of glucose, L‐glutamate, and acetylcholine using microdialysis probes and immobilized enzyme reactors