CA1101348A - Activation of immobilized living microorganisms - Google Patents

Activation of immobilized living microorganisms

Info

Publication number: CA1101348A
Authority: CA; Canada
Prior art keywords: peptone; glucose; immobilized; cortisol; transformation
Prior art date: 1976-07-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA282,058A

Other languages

English (en)

French (fr)

Inventor

Klaus H. Mosbach

Per-Olof Larsson

Sten A. Ohlson

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Fermenta AB

Original Assignee

Fermenta AB

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1976-07-06

Filing date

1977-07-05

Publication date

1981-05-19

1977-07-05 Application filed by Fermenta AB filed Critical Fermenta AB

1981-05-19 Application granted granted Critical

1981-05-19 Publication of CA1101348A publication Critical patent/CA1101348A/en

Status Expired legal-status Critical Current

Links

244000005700 microbiome Species 0.000 title claims abstract description 15
230000004913 activation Effects 0.000 title description 5
230000009466 transformation Effects 0.000 claims abstract description 21
238000000034 method Methods 0.000 claims abstract description 20
239000001888 Peptone Substances 0.000 claims abstract description 19
108010080698 Peptones Proteins 0.000 claims abstract description 19
235000019319 peptone Nutrition 0.000 claims abstract description 19
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 18
239000008103 glucose Substances 0.000 claims abstract description 18
150000003431 steroids Chemical class 0.000 claims abstract description 11
229920000642 polymer Polymers 0.000 claims abstract description 7
230000003213 activating effect Effects 0.000 claims abstract description 6
239000003242 anti bacterial agent Substances 0.000 claims abstract description 5
229940088710 antibiotic agent Drugs 0.000 claims abstract description 5
239000000203 mixture Substances 0.000 claims abstract description 5
238000000844 transformation Methods 0.000 claims abstract description 5
239000011541 reaction mixture Substances 0.000 claims abstract description 4
JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 30
229960000890 hydrocortisone Drugs 0.000 claims description 15
238000005805 hydroxylation reaction Methods 0.000 claims description 9
OIGNJSKKLXVSLS-VWUMJDOOSA-N prednisolone Chemical compound O=C1C=C[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OIGNJSKKLXVSLS-VWUMJDOOSA-N 0.000 claims description 8
229960005205 prednisolone Drugs 0.000 claims description 7
241000203720 Pimelobacter simplex Species 0.000 claims description 5
238000006356 dehydrogenation reaction Methods 0.000 claims description 4
229920002401 polyacrylamide Polymers 0.000 claims description 4
241000284466 Antarctothoa delta Species 0.000 claims 1
230000000694 effects Effects 0.000 description 15
239000000499 gel Substances 0.000 description 15
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 10
239000002609 medium Substances 0.000 description 8
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 5
229910000396 dipotassium phosphate Inorganic materials 0.000 description 5
235000019797 dipotassium phosphate Nutrition 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
229940056360 penicillin g Drugs 0.000 description 5
241000430521 Alyssum Species 0.000 description 4
239000003054 catalyst Substances 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
238000010348 incorporation Methods 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
239000003246 corticosteroid Substances 0.000 description 3
-1 corticosteroids Chemical class 0.000 description 3
125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
235000015097 nutrients Nutrition 0.000 description 3
230000008569 process Effects 0.000 description 3
150000003839 salts Chemical class 0.000 description 3
WHBHBVVOGNECLV-OBQKJFGGSA-N 11-deoxycortisol Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 WHBHBVVOGNECLV-OBQKJFGGSA-N 0.000 description 2
JYGXADMDTFJGBT-MKIDGPAKSA-N 11alpha-Hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-MKIDGPAKSA-N 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
SVTBMSDMJJWYQN-UHFFFAOYSA-N 2-methylpentane-2,4-diol Chemical compound CC(O)CC(C)(C)O SVTBMSDMJJWYQN-UHFFFAOYSA-N 0.000 description 2
NGHVIOIJCVXTGV-ALEPSDHESA-N 6-aminopenicillanic acid Chemical compound [O-]C(=O)[C@H]1C(C)(C)S[C@@H]2[C@H]([NH3+])C(=O)N21 NGHVIOIJCVXTGV-ALEPSDHESA-N 0.000 description 2
NGHVIOIJCVXTGV-UHFFFAOYSA-N 6beta-amino-penicillanic acid Natural products OC(=O)C1C(C)(C)SC2C(N)C(=O)N21 NGHVIOIJCVXTGV-UHFFFAOYSA-N 0.000 description 2
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
102000008186 Collagen Human genes 0.000 description 2
108010035532 Collagen Proteins 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 description 2
230000008901 benefit Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
229960005188 collagen Drugs 0.000 description 2
150000001886 cortisols Chemical class 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
239000008187 granular material Substances 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
238000006116 polymerization reaction Methods 0.000 description 2
239000000047 product Substances 0.000 description 2
239000000725 suspension Substances 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
XHRJGHCQQPETRH-KQYNXXCUSA-N (2r,3r,4s,5r)-2-(6-chloropurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(Cl)=C2N=C1 XHRJGHCQQPETRH-KQYNXXCUSA-N 0.000 description 1
BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 description 1
ZKBQDFAWXLTYKS-UHFFFAOYSA-N 6-Chloro-1H-purine Chemical compound ClC1=NC=NC2=C1NC=N2 ZKBQDFAWXLTYKS-UHFFFAOYSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
241000228245 Aspergillus niger Species 0.000 description 1
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
241000186146 Brevibacterium Species 0.000 description 1
239000004380 Cholic acid Substances 0.000 description 1
241000186216 Corynebacterium Species 0.000 description 1
241000223211 Curvularia lunata Species 0.000 description 1
101710088194 Dehydrogenase Proteins 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
101100521130 Mus musculus Prelid1 gene Proteins 0.000 description 1
KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
125000003047 N-acetyl group Chemical group 0.000 description 1
241000235527 Rhizopus Species 0.000 description 1
241000235546 Rhizopus stolonifer Species 0.000 description 1
XYNPYHXGMWJBLV-VXPJTDKGSA-N Tomatidine Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)CC[C@H](O)C[C@@H]4CC[C@H]3[C@@H]2C1)C)[C@@H]1C)[C@@]11CC[C@H](C)CN1 XYNPYHXGMWJBLV-VXPJTDKGSA-N 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
JIAARYAFYJHUJI-UHFFFAOYSA-L Zinc chloride Inorganic materials [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
239000008272 agar Substances 0.000 description 1
229930013930 alkaloid Natural products 0.000 description 1
AEMFNILZOJDQLW-QAGGRKNESA-N androst-4-ene-3,17-dione Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 AEMFNILZOJDQLW-QAGGRKNESA-N 0.000 description 1
229960005471 androstenedione Drugs 0.000 description 1
AEMFNILZOJDQLW-UHFFFAOYSA-N androstenedione Natural products O=C1CCC2(C)C3CCC(C)(C(CC4)=O)C4C3CCC2=C1 AEMFNILZOJDQLW-UHFFFAOYSA-N 0.000 description 1
239000002585 base Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
235000010410 calcium alginate Nutrition 0.000 description 1
239000000648 calcium alginate Substances 0.000 description 1
229960002681 calcium alginate Drugs 0.000 description 1
OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
RUDATBOHQWOJDD-BSWAIDMHSA-N chenodeoxycholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)CC1 RUDATBOHQWOJDD-BSWAIDMHSA-N 0.000 description 1
229960001091 chenodeoxycholic acid Drugs 0.000 description 1
BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
235000019416 cholic acid Nutrition 0.000 description 1
229960002471 cholic acid Drugs 0.000 description 1
229920001436 collagen Polymers 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
229960001334 corticosteroids Drugs 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
229910001873 dinitrogen Inorganic materials 0.000 description 1
238000001914 filtration Methods 0.000 description 1
238000005755 formation reaction Methods 0.000 description 1
OSVMTWJCGUFAOD-KZQROQTASA-N formestane Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1O OSVMTWJCGUFAOD-KZQROQTASA-N 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000006481 glucose medium Substances 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000013383 initial experiment Methods 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000006317 isomerization reaction Methods 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
230000004807 localization Effects 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
239000000178 monomer Substances 0.000 description 1
239000002777 nucleoside Substances 0.000 description 1
125000003835 nucleoside group Chemical group 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
235000005985 organic acids Nutrition 0.000 description 1
235000019371 penicillin G benzathine Nutrition 0.000 description 1
JRKICGRDRMAZLK-UHFFFAOYSA-L persulfate group Chemical group S(=O)(=O)([O-])OOS(=O)(=O)[O-] JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
238000006722 reduction reaction Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
PWRIIDWSQYQFQD-UHFFFAOYSA-N sisunine Natural products CC1CCC2(NC1)OC3CC4C5CCC6CC(CCC6(C)C5CCC4(C)C3C2C)OC7OC(CO)C(OC8OC(CO)C(O)C(OC9OC(CO)C(O)C(O)C9OC%10OC(CO)C(O)C(O)C%10O)C8O)C(O)C7O PWRIIDWSQYQFQD-UHFFFAOYSA-N 0.000 description 1
KWVISVAMQJWJSZ-VKROHFNGSA-N solasodine Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)CC[C@H](O)CC4=CC[C@H]3[C@@H]2C1)C)[C@@H]1C)[C@]11CC[C@@H](C)CN1 KWVISVAMQJWJSZ-VKROHFNGSA-N 0.000 description 1
JXWLYDNHVXFBJA-UHFFFAOYSA-N solasodine Natural products CC1CCC2(NC1)NC3CC4C5CC=C6CC(O)CCC6(C)C5CCC4(C)C3C2C JXWLYDNHVXFBJA-UHFFFAOYSA-N 0.000 description 1
239000000243 solution Substances 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000013589 supplement Substances 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
XYNPYHXGMWJBLV-OFMODGJOSA-N tomatidine Natural products O[C@@H]1C[C@H]2[C@@](C)([C@@H]3[C@H]([C@H]4[C@@](C)([C@H]5[C@@H](C)[C@]6(O[C@H]5C4)NC[C@@H](C)CC6)CC3)CC2)CC1 XYNPYHXGMWJBLV-OFMODGJOSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
235000012711 vitamin K3 Nutrition 0.000 description 1
239000011652 vitamin K3 Substances 0.000 description 1
229940041603 vitamin k 3 Drugs 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Health & Medical Sciences (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Mycology (AREA)
Dispersion Chemistry (AREA)
Biomedical Technology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA282,058A 1976-07-06 1977-07-05 Activation of immobilized living microorganisms Expired CA1101348A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
SE7607698A SE427116B (sv)	1976-07-06	1976-07-06	Transformation av steroider och antibiotika med immobiliserade levande mikroorganismer som aktiveras
SE7607698-3		1976-07-06

Publications (1)

Publication Number	Publication Date
CA1101348A true CA1101348A (en)	1981-05-19

Family

ID=20328400

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA282,058A Expired CA1101348A (en)	1976-07-06	1977-07-05	Activation of immobilized living microorganisms

Country Status (15)

Country	Link
JP (1)	JPS5326383A (de)
AT (1)	AT372403B (de)
BE (1)	BE856358A (de)
CA (1)	CA1101348A (de)
CH (1)	CH634348A5 (de)
DE (1)	DE2729490A1 (de)
DK (1)	DK292677A (de)
ES (1)	ES460368A1 (de)
FI (1)	FI58941C (de)
FR (1)	FR2357507A1 (de)
GB (1)	GB1560850A (de)
IE (1)	IE45676B1 (de)
IT (1)	IT1079745B (de)
NL (1)	NL7707449A (de)
SE (1)	SE427116B (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SE441009B (sv) *	1982-03-08	1985-09-02	Kjell Nilsson	Sett att immobilisera levande biomaterial i perlformiga polymerer
DE3241829A1 (de) *	1982-11-09	1984-05-10	Schering AG, 1000 Berlin und 4709 Bergkamen	Biokatalysator
GB2162198B (en) *	1984-07-27	1987-12-02	Ki Med I	Process for producing dense nutrient medium for culturing microorganisms and macroorganism cell cultures
GB9622884D0 (en) *	1996-11-02	1997-01-08	Duramed Europ Ltd	A method for th e preparation of steroids in the pregene class

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3767790A (en) *	1972-02-11	1973-10-23	Nat Patent Dev Corp	Microorganisms
FR2320349A1 (fr) *	1975-08-06	1977-03-04	Agronomique Inst Nat Rech	Procede enzymatique utilisant des microorganismes inclus

1976
- 1976-07-06 SE SE7607698A patent/SE427116B/xx unknown
1977
- 1977-06-28 FI FI772012A patent/FI58941C/fi not_active IP Right Cessation
- 1977-06-30 DK DK292677A patent/DK292677A/da not_active Application Discontinuation
- 1977-06-30 DE DE19772729490 patent/DE2729490A1/de not_active Ceased
- 1977-06-30 AT AT0465577A patent/AT372403B/de not_active IP Right Cessation
- 1977-07-01 BE BE178985A patent/BE856358A/xx not_active IP Right Cessation
- 1977-07-04 ES ES460368A patent/ES460368A1/es not_active Expired
- 1977-07-05 CA CA282,058A patent/CA1101348A/en not_active Expired
- 1977-07-05 NL NL7707449A patent/NL7707449A/xx not_active Application Discontinuation
- 1977-07-05 IT IT50134/77A patent/IT1079745B/it active
- 1977-07-05 FR FR7720678A patent/FR2357507A1/fr active Granted
- 1977-07-05 IE IE1392/77A patent/IE45676B1/en unknown
- 1977-07-05 GB GB28177/77A patent/GB1560850A/en not_active Expired
- 1977-07-06 CH CH835577A patent/CH634348A5/de not_active IP Right Cessation
- 1977-07-06 JP JP8086277A patent/JPS5326383A/ja active Pending

Also Published As

Publication number	Publication date
FI58941C (fi)	1981-05-11
FI58941B (fi)	1981-01-30
DE2729490A1 (de)	1978-01-12
BE856358A (fr)	1978-01-02
IE45676L (en)	1978-01-06
FI772012A7 (de)	1978-01-07
FR2357507A1 (fr)	1978-02-03
SE7607698L (sv)	1978-01-07
SE427116B (sv)	1983-03-07
JPS5326383A (en)	1978-03-11
CH634348A5 (en)	1983-01-31
NL7707449A (nl)	1978-01-10
DK292677A (da)	1978-01-07
GB1560850A (en)	1980-02-13
AT372403B (de)	1983-10-10
ATA465577A (de)	1983-02-15
IE45676B1 (en)	1982-10-20
IT1079745B (it)	1985-05-13
FR2357507B1 (de)	1981-10-16
ES460368A1 (es)	1978-10-01

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Date	Code	Title	Description
1998-05-19	MKEX	Expiry

Publication	Publication Date	Title
Kaboli et al.	1980	Immobilization and properties of Leuconostoc mesenteroids dextransucrase
Koshcheyenko et al.	1983	Immobilization of living microbial cells and their application for steroid transformations
Ohlson et al.	1980	Steroid hydroxylation using immobilized spores of Curvularia lunata germinated in situ
EP0024182A2 (de)	1981-02-25	Thermostabiler Mikroorganismus und daraus hergestelltes proteolytisches Enzym und Verfahren zur Herstellung des Mikroorganismus und des proteolytischen Enzyms
CA1101348A (en)	1981-05-19	Activation of immobilized living microorganisms
Sonomoto et al.	1983	9α-Hydroxylation of 4-androstene-3, 17-dione by gel-entrapped Corynebacterium sp. cells
JPH06225792A (ja)	1994-08-16	ステロイド転化プロセス
US3843442A (en)	1974-10-22	Immobilized glucose isomerase
JP3858505B2 (ja)	2006-12-13	Ｒ−３−キヌクリジノールの製造方法
IE841198L (en)	1984-11-16	Dehydrogenation of steroids
Kloosterman IV et al.	1984	Effect of supersaturated aqueous hydrocortisone concentrations on the Δ1-dehydrogenase activity of free and immobilized Arthrobacter simplex
CA1043723A (en)	1978-12-05	Production of cholesterol oxidase by nocardia cholesterolicum
US4246346A (en)	1981-01-20	Antibiotic and steroid transformation process
US4283496A (en)	1981-08-11	Preparation and use of glucose isomerase
US4546078A (en)	1985-10-08	Polymer containing biocatalyst
US3360439A (en)	1967-12-26	Process for preparing 1-dehydro steroids
EP0032987B1 (de)	1986-05-28	Thermophilische Aspartase, Verfahren zur Herstellung, verwendete Kultur und sie verwendendes Verfahren zur Herstellung von L-Asparaginsäure
Kim et al.	1982	Steroid modification with immobilized mycelium of Aspergillus phoenicis
US4061539A (en)	1977-12-06	Preparation and use of glucose isomerase
US5004695A (en)	1991-04-02	Microbiological process for preparing 9α-hydroxy-4-androstene-3,17-dione
JP3691875B2 (ja)	2005-09-07	耐熱性マルトースホスホリラーゼ、その製造方法、その製造に使用する菌、および該酵素の使用方法
CA1069073A (en)	1980-01-01	Method for the preparation of cholesterol oxidase
US3878046A (en)	1975-04-15	Hydroxylated prostaglandins and process
Atrat et al.	1981	Steroid transformation with immobilized microorganisms: V. Continuous side chain cleavage of a cholesterol derivative with immobilized Mycobacterium phlei cells
US2991230A (en)	1961-07-04	Oxygenation of steroids with streptomyces halstedii