CA1123733A - Antigene de l'hepatite-b et anticorps sous forme insoluble - Google Patents

Antigene de l'hepatite-b et anticorps sous forme insoluble

Info

Publication number: CA1123733A
Authority: CA; Canada
Prior art keywords: hbeag; hbe; reagent; insoluble form; labeled
Prior art date: 1977-12-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA313,316A

Other languages

English (en)

Inventor

Isa K. Mushahwar

Chung-Mei Ling

Lacy R. Overby

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1977-12-14

Filing date

1978-10-13

Publication date

1982-05-18

1978-10-13 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

1982-05-18 Application granted granted Critical

1982-05-18 Publication of CA1123733A publication Critical patent/CA1123733A/fr

Status Expired legal-status Critical Current

Links

239000000427 antigen Substances 0.000 title abstract description 11
208000006454 hepatitis Diseases 0.000 title 1
231100000283 hepatitis Toxicity 0.000 title 1
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 18
238000001514 detection method Methods 0.000 claims abstract description 12
239000007787 solid Substances 0.000 claims abstract description 9
239000003446 ligand Substances 0.000 claims abstract description 6
239000011324 bead Substances 0.000 claims description 26
210000002966 serum Anatomy 0.000 claims description 12
241001465754 Metazoa Species 0.000 claims description 7
102000004190 Enzymes Human genes 0.000 claims description 4
108090000790 Enzymes Proteins 0.000 claims description 4
PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 4
108010001336 Horseradish Peroxidase Proteins 0.000 claims description 2
230000003053 immunization Effects 0.000 claims description 2
238000002649 immunization Methods 0.000 claims description 2
101710142246 External core antigen Proteins 0.000 claims 8
230000001900 immune effect Effects 0.000 claims 2
208000002672 hepatitis B Diseases 0.000 abstract description 6
238000003018 immunoassay Methods 0.000 abstract description 3
238000000034 method Methods 0.000 description 17
239000000523 sample Substances 0.000 description 16
239000000243 solution Substances 0.000 description 12
238000003127 radioimmunoassay Methods 0.000 description 7
238000005406 washing Methods 0.000 description 6
108060003951 Immunoglobulin Proteins 0.000 description 5
230000000951 immunodiffusion Effects 0.000 description 5
102000018358 immunoglobulin Human genes 0.000 description 5
238000002360 preparation method Methods 0.000 description 5
102000036639 antigens Human genes 0.000 description 3
108091007433 antigens Proteins 0.000 description 3
238000003556 assay Methods 0.000 description 3
239000000872 buffer Substances 0.000 description 3
239000000969 carrier Substances 0.000 description 3
239000013642 negative control Substances 0.000 description 3
239000007790 solid phase Substances 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
229920002684 Sepharose Polymers 0.000 description 2
241000473945 Theria <moth genus> Species 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
229910052921 ammonium sulfate Inorganic materials 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000002372 labelling Methods 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
238000004393 prognosis Methods 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
229920001817 Agar Polymers 0.000 description 1
102000009027 Albumins Human genes 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
241000700198 Cavia Species 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
102000006395 Globulins Human genes 0.000 description 1
108010044091 Globulins Proteins 0.000 description 1
206010067125 Liver injury Diseases 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
238000013459 approach Methods 0.000 description 1
239000012062 aqueous buffer Substances 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
244000309466 calf Species 0.000 description 1
VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 1
231100000749 chronicity Toxicity 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000011521 glass Substances 0.000 description 1
231100000234 hepatic damage Toxicity 0.000 description 1
238000000760 immunoelectrophoresis Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
ZCYVEMRRCGMTRW-YPZZEJLDSA-N iodine-125 Chemical compound [125I] ZCYVEMRRCGMTRW-YPZZEJLDSA-N 0.000 description 1
229940044173 iodine-125 Drugs 0.000 description 1
230000008818 liver damage Effects 0.000 description 1
208000019423 liver disease Diseases 0.000 description 1
239000003550 marker Substances 0.000 description 1
239000000203 mixture Substances 0.000 description 1
238000006386 neutralization reaction Methods 0.000 description 1
239000012071 phase Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000007858 starting material Substances 0.000 description 1
229960001479 tosylchloramide sodium Drugs 0.000 description 1
230000003612 virological effect Effects 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5761—Hepatitis B

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Urology & Nephrology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Food Science & Technology (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Communicable Diseases (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

CA313,316A 1977-12-14 1978-10-13 Antigene de l'hepatite-b et anticorps sous forme insoluble Expired CA1123733A (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US86053077A	1977-12-14	1977-12-14
US860,530		1977-12-14

Publications (1)

Publication Number	Publication Date
CA1123733A true CA1123733A (fr)	1982-05-18

Family

ID=25333425

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA313,316A Expired CA1123733A (fr)	1977-12-14	1978-10-13	Antigene de l'hepatite-b et anticorps sous forme insoluble

Country Status (9)

Country	Link
JP (1)	JPS5487289A (fr)
AU (1)	AU4085578A (fr)
CA (1)	CA1123733A (fr)
DE (1)	DE2853859A1 (fr)
FR (1)	FR2412075A1 (fr)
GB (1)	GB2010480B (fr)
IT (1)	IT1101728B (fr)
NL (1)	NL7812142A (fr)
SE (1)	SE7812663L (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5726011A (en) *	1994-03-31	1998-03-10	Virginia Commonwealth University	Method for diagnosing chronic hepatitis B virus infection
WO1997035204A1 (fr) *	1996-03-19	1997-09-25	Akzo Nobel N.V.	Procede de pronostic de l'evolution d'une hepatite b

1978
- 1978-10-13 CA CA313,316A patent/CA1123733A/fr not_active Expired
- 1978-10-18 GB GB7840962A patent/GB2010480B/en not_active Expired
- 1978-10-19 AU AU40855/78A patent/AU4085578A/en active Pending
- 1978-11-17 JP JP14125678A patent/JPS5487289A/ja active Pending
- 1978-12-08 SE SE7812663A patent/SE7812663L/xx unknown
- 1978-12-13 DE DE19782853859 patent/DE2853859A1/de active Pending
- 1978-12-13 NL NL7812142A patent/NL7812142A/xx not_active Application Discontinuation
- 1978-12-13 FR FR7835111A patent/FR2412075A1/fr not_active Withdrawn
- 1978-12-13 IT IT30790/78A patent/IT1101728B/it active

Also Published As

Publication number	Publication date
AU4085578A (en)	1980-04-24
IT7830790A0 (it)	1978-12-13
FR2412075A1 (fr)	1979-07-13
JPS5487289A (en)	1979-07-11
NL7812142A (nl)	1979-06-18
IT1101728B (it)	1985-10-07
SE7812663L (sv)	1979-06-15
GB2010480A (en)	1979-06-27
GB2010480B (en)	1982-07-14
DE2853859A1 (de)	1979-06-28

Legal Events

Date	Code	Title	Description
1999-05-18	MKEX	Expiry

Publication	Publication Date	Title
Yolken	1980	Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents
Selby	1999	Interference in immunoassay
Yorde et al.	1976	Competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. I. Measurement of human choriogonadotropin.
US5011771A (en)	1991-04-30	Multiepitopic immunometric assay
CA1148859A (fr)	1983-06-28	Technique pour le depistage simultane de deux virus de l'hepatite dans un milieu solide
Neurath et al.	1981	Enzyme-linked fluorescence immunoassays using β-galactosidase and antibodies covalently bound to polystyrene plates
US4514507A (en)	1985-04-30	Assay for interferon
JPH08101197A (ja)	1996-04-16	制御された感度の免疫クロマトグラフィー検定
DK165381B (da)	1992-11-16	Immunoanalysemetode til bestemmelse af ligander ved hjaelp af biotin og antibiotin
O'Keefe et al.	1980	Use of immunoglobulin-loaded protein A-bearing staphylococci as a primary solid phase immunoadsorbent in radioimmunoassay.
EP0149602A1 (fr)	1985-07-31	Analyse immunometrique utilisant des anticorps polyclonaux et monoclonaux et kit d'analyse
CN113030461B (zh)	2022-04-22	含氨基或羧基基团物质标记的IgG抗体、人IgG磁微粒化学检测试剂盒及其制备方法
JPS6171360A (ja)	1986-04-12	血清中の抗原及び抗体の同時検定のためのアツセイ
US4189464A (en)	1980-02-19	Hepatitis B testing reagent and method
JP2006522736A (ja)	2006-10-05	アシアロα１−酸性糖タンパクに対するモノクロナール抗体、モノクロナール抗体からなる免疫クロマトグラフィ用帯状片及びこの免疫クロマトグラフィ用帯状片を用いる肝疾患診断法
EP0103605A1 (fr)	1984-03-28	Procede pour analyses de groupes de coordination libres.
US4837167A (en)	1989-06-06	Immunoassay for multi-determinant antigens using high-affinity
EP0071635A1 (fr)	1983-02-16	Essai immunologique pour antigenes multi-determinants.
CA1123733A (fr)	1982-05-18	Antigene de l'hepatite-b et anticorps sous forme insoluble
US5304498A (en)	1994-04-19	Method and composition for free ligand assay
Wu et al.	1999	Time-resolved fluorescence immunoassay of thyroxine in serum: immobilized antigen approach
Rizzetto et al.	1981	A radioimmunoassay for HBcAg in the sera of HBsAg carriers: Serum HBcAg, serum DNA polymerase activity, and liver HBcAg immunofluorescence as markers of chronic liver disease
IE60253B1 (en)	1994-06-29	"Membrane affinity concentration immunoassay"
JP3502497B2 (ja)	2004-03-02	複合化被検物質誘導体を用いた競合イムノアッセイ
Vonk et al.	1991	Dual-enzyme cascade-magnetic separation immunoassay for respiratory syncytial virus