CA1127055A - Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation - Google Patents

Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation

Info

Publication number: CA1127055A
Authority: CA; Canada
Prior art keywords: enzyme; stabilized; solution; composition; organic solvent
Prior art date: 1976-09-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA372,912A

Other languages

English (en)

Inventor

Ivan E. Modrovich

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1976-09-13

Filing date

1981-03-12

Publication date

1982-07-06

1977-08-31 Priority claimed from CA285,845A external-priority patent/CA1102225A/fr

1981-03-12 Application filed by Individual filed Critical Individual

1981-03-12 Priority to CA372,912A priority Critical patent/CA1127055A/fr

1982-07-06 Application granted granted Critical

1982-07-06 Publication of CA1127055A publication Critical patent/CA1127055A/fr

Status Expired legal-status Critical Current

Links

102000004190 Enzymes Human genes 0.000 title claims abstract description 85
108090000790 Enzymes Proteins 0.000 title claims abstract description 85
239000000203 mixture Substances 0.000 title claims abstract description 32
239000007788 liquid Substances 0.000 title claims description 27
238000000034 method Methods 0.000 title claims description 15
239000005515 coenzyme Substances 0.000 title abstract description 57
229920000642 polymer Polymers 0.000 claims abstract description 26
-1 azide compound Chemical class 0.000 claims abstract description 21
239000003960 organic solvent Substances 0.000 claims abstract description 19
102000005548 Hexokinase Human genes 0.000 claims abstract description 13
108700040460 Hexokinases Proteins 0.000 claims abstract description 13
239000012736 aqueous medium Substances 0.000 claims abstract description 5
230000002255 enzymatic effect Effects 0.000 claims abstract description 4
230000006870 function Effects 0.000 claims abstract description 4
239000003381 stabilizer Substances 0.000 claims abstract description 4
238000006243 chemical reaction Methods 0.000 claims description 43
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
230000006641 stabilisation Effects 0.000 claims description 17
238000011105 stabilization Methods 0.000 claims description 17
230000000087 stabilizing effect Effects 0.000 claims description 8
102000004420 Creatine Kinase Human genes 0.000 claims description 7
108010042126 Creatine kinase Proteins 0.000 claims description 7
239000000022 bacteriostatic agent Substances 0.000 claims description 5
102100031126 6-phosphogluconolactonase Human genes 0.000 claims description 3
108010029731 6-phosphogluconolactonase Proteins 0.000 claims description 3
101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims description 3
108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims description 3
102000016901 Glutamate dehydrogenase Human genes 0.000 claims description 3
102000013009 Pyruvate Kinase Human genes 0.000 claims description 3
108020005115 Pyruvate Kinase Proteins 0.000 claims description 3
101000833181 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Glycerol dehydrogenase 1 Proteins 0.000 claims description 3
238000007789 sealing Methods 0.000 claims description 3
229920003169 water-soluble polymer Polymers 0.000 claims description 3
229910052739 hydrogen Inorganic materials 0.000 claims description 2
239000001257 hydrogen Substances 0.000 claims description 2
125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 2
238000003797 solvolysis reaction Methods 0.000 claims description 2
239000008135 aqueous vehicle Substances 0.000 claims 4
239000003513 alkali Substances 0.000 claims 2
102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims 1
108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
230000003385 bacteriostatic effect Effects 0.000 claims 1
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 abstract description 13
230000015556 catabolic process Effects 0.000 abstract description 8
238000006731 degradation reaction Methods 0.000 abstract description 8
101710088194 Dehydrogenase Proteins 0.000 abstract description 5
BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract description 3
229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 abstract description 2
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 2
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 2
230000036425 denaturation Effects 0.000 abstract 1
238000004925 denaturation Methods 0.000 abstract 1
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 54
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 44
229950006238 nadide Drugs 0.000 description 34
239000000243 solution Substances 0.000 description 32
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 30
CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 14
239000000758 substrate Substances 0.000 description 14
239000003153 chemical reaction reagent Substances 0.000 description 11
101100080807 Drosophila melanogaster mt:ND2 gene Proteins 0.000 description 10
101150016680 MT-ND2 gene Proteins 0.000 description 10
102100028488 NADH-ubiquinone oxidoreductase chain 2 Human genes 0.000 description 10
101150102231 ND2 gene Proteins 0.000 description 10
108010010803 Gelatin Proteins 0.000 description 9
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 9
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 9
229920000159 gelatin Polymers 0.000 description 9
239000008273 gelatin Substances 0.000 description 9
235000019322 gelatine Nutrition 0.000 description 9
235000011852 gelatine desserts Nutrition 0.000 description 9
239000008103 glucose Substances 0.000 description 9
229920005862 polyol Polymers 0.000 description 9
150000003077 polyols Chemical class 0.000 description 9
239000002904 solvent Substances 0.000 description 9
OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 7
229960003624 creatine Drugs 0.000 description 7
239000006046 creatine Substances 0.000 description 7
239000003795 chemical substances by application Substances 0.000 description 6
239000000047 product Substances 0.000 description 6
DTBNBXWJWCWCIK-UHFFFAOYSA-N Phosphoenolpyruvic acid Natural products OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 5
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 5
238000005859 coupling reaction Methods 0.000 description 5
239000011521 glass Substances 0.000 description 5
150000003839 salts Chemical group 0.000 description 5
239000000126 substance Substances 0.000 description 5
DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 description 4
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
239000007864 aqueous solution Substances 0.000 description 4
230000008878 coupling Effects 0.000 description 4
238000010168 coupling process Methods 0.000 description 4
230000000694 effects Effects 0.000 description 4
230000008569 process Effects 0.000 description 4
229960004063 propylene glycol Drugs 0.000 description 4
235000013772 propylene glycol Nutrition 0.000 description 4
238000005057 refrigeration Methods 0.000 description 4
239000000725 suspension Substances 0.000 description 4
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 3
238000003556 assay Methods 0.000 description 3
230000003139 buffering effect Effects 0.000 description 3
239000000470 constituent Substances 0.000 description 3
239000013256 coordination polymer Substances 0.000 description 3
238000006911 enzymatic reaction Methods 0.000 description 3
239000011159 matrix material Substances 0.000 description 3
230000007246 mechanism Effects 0.000 description 3
238000004806 packaging method and process Methods 0.000 description 3
BIRSGZKFKXLSJQ-SQOUGZDYSA-N 6-Phospho-D-gluconate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O BIRSGZKFKXLSJQ-SQOUGZDYSA-N 0.000 description 2
108010082126 Alanine transaminase Proteins 0.000 description 2
101000878595 Arabidopsis thaliana Squalene synthase 1 Proteins 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
229920002307 Dextran Polymers 0.000 description 2
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
108010046334 Urease Proteins 0.000 description 2
239000002253 acid Substances 0.000 description 2
230000002378 acidificating effect Effects 0.000 description 2
230000000844 anti-bacterial effect Effects 0.000 description 2
150000001540 azides Chemical class 0.000 description 2
WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000000337 buffer salt Substances 0.000 description 2
239000006172 buffering agent Substances 0.000 description 2
230000003197 catalytic effect Effects 0.000 description 2
230000008859 change Effects 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
230000003993 interaction Effects 0.000 description 2
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
229910052749 magnesium Inorganic materials 0.000 description 2
239000011777 magnesium Substances 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000002156 mixing Methods 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
IZUPBVBPLAPZRR-UHFFFAOYSA-N pentachlorophenol Chemical compound OC1=C(Cl)C(Cl)=C(Cl)C(Cl)=C1Cl IZUPBVBPLAPZRR-UHFFFAOYSA-N 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
229920003023 plastic Polymers 0.000 description 2
230000009257 reactivity Effects 0.000 description 2
239000007787 solid Substances 0.000 description 2
HHVIBTZHLRERCL-UHFFFAOYSA-N sulfonyldimethane Chemical compound CS(C)(=O)=O HHVIBTZHLRERCL-UHFFFAOYSA-N 0.000 description 2
MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 2
DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 1
102100036475 Alanine aminotransferase 1 Human genes 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
208000031295 Animal disease Diseases 0.000 description 1
108010003415 Aspartate Aminotransferases Proteins 0.000 description 1
102000004625 Aspartate Aminotransferases Human genes 0.000 description 1
239000005711 Benzoic acid Substances 0.000 description 1
LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
101100459438 Caenorhabditis elegans nac-1 gene Proteins 0.000 description 1
102000008186 Collagen Human genes 0.000 description 1
108010035532 Collagen Proteins 0.000 description 1
NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 1
RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
241000233866 Fungi Species 0.000 description 1
VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 1
101710198413 Hexokinase-8 Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
239000007990 PIPES buffer Substances 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
239000005844 Thymol Substances 0.000 description 1
108090000340 Transaminases Proteins 0.000 description 1
102000003929 Transaminases Human genes 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
150000001298 alcohols Chemical class 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000008044 alkali metal hydroxides Chemical class 0.000 description 1
238000013459 approach Methods 0.000 description 1
239000003899 bactericide agent Substances 0.000 description 1
239000002585 base Substances 0.000 description 1
235000010233 benzoic acid Nutrition 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
150000003841 chloride salts Chemical class 0.000 description 1
229920001436 collagen Polymers 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000001177 diphosphate Substances 0.000 description 1
230000008034 disappearance Effects 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
150000002170 ethers Chemical class 0.000 description 1
239000012467 final product Substances 0.000 description 1
239000012530 fluid Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
239000000417 fungicide Substances 0.000 description 1
229940050410 gluconate Drugs 0.000 description 1
125000005639 glycero group Chemical group 0.000 description 1
229960005150 glycerol Drugs 0.000 description 1
235000011187 glycerol Nutrition 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
150000004000 hexols Chemical class 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
150000002576 ketones Chemical class 0.000 description 1
239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
239000006193 liquid solution Substances 0.000 description 1
230000007774 longterm Effects 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
238000011177 media preparation Methods 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
244000005700 microbiome Species 0.000 description 1
229940099990 ogen Drugs 0.000 description 1
239000011368 organic material Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
229930029653 phosphoenolpyruvate Natural products 0.000 description 1
229920006316 polyvinylpyrrolidine Polymers 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000003908 quality control method Methods 0.000 description 1
230000036647 reaction Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000011877 solvent mixture Substances 0.000 description 1
230000003068 static effect Effects 0.000 description 1
150000003457 sulfones Chemical class 0.000 description 1
150000003462 sulfoxides Chemical class 0.000 description 1
125000003831 tetrazolyl group Chemical group 0.000 description 1
229960000790 thymol Drugs 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA372,912A 1976-09-13 1981-03-12 Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation Expired CA1127055A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA372,912A CA1127055A (fr)	1976-09-13	1981-03-12	Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US72256576A	1976-09-13	1976-09-13
US722,565		1976-09-13
CA285,845A CA1102225A (fr)	1976-09-13	1977-08-31	Compose liquide stabilise contenant une enzyme et une coenzyme et methode de preparation
CA372,912A CA1127055A (fr)	1976-09-13	1981-03-12	Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation

Publications (1)

Publication Number	Publication Date
CA1127055A true CA1127055A (fr)	1982-07-06

Family

ID=27165262

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA372,912A Expired CA1127055A (fr)	1976-09-13	1981-03-12	Compose liquide contenant un enzyme et un coenzyme stabilises et methode de preparation

Country Status (1)

Country	Link
CA (1)	CA1127055A (fr)

1981
- 1981-03-12 CA CA372,912A patent/CA1127055A/fr not_active Expired

Legal Events

Date	Code	Title	Description
1999-07-06	MKEX	Expiry
2023-05-26	MKEX	Expiry	Effective date: 19990706

Publication	Publication Date	Title
US4271264A (en)	1981-06-02	Stabilized liquid enzyme and coenzyme compositions
US4250254A (en)	1981-02-10	Stabilized liquid enzyme and coenzyme compositions
CA1178875A (fr)	1984-12-04	Stabilisation de co-enzymes en solution aqueuse
US4372874A (en)	1983-02-08	Stabilization of hydrolysis prone labile organic reagents in liquid media
US4277562A (en)	1981-07-07	Stabilized liquid enzyme and coenzyme compositions
EP0072581B1 (fr)	1987-04-08	Stabilisation de solutions de réactifs prêtes à l'emploi contenant des enzymes et usage de ces réactifs stabilisés dans les essais d'enzymes ou de substrats
US3764478A (en)	1973-10-09	Stabilized enzymatic test reagents
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