CA1128917A - Matrice de support pour enzymes immobilises - Google Patents

Matrice de support pour enzymes immobilises

Info

Publication number: CA1128917A
Authority: CA; Canada
Prior art keywords: matrix; set forth; enzyme; solid support; aminopolystyrene
Prior art date: 1978-10-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA337,572A

Other languages

English (en)

Inventor

Ronald P. Rohrbach

Joseph Levy

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Honeywell UOP LLC

Original Assignee

UOP LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-10-16

Filing date

1979-10-15

Publication date

1982-08-03

1978-10-16 Priority claimed from US05/951,948 external-priority patent/US4193910A/en

1978-10-16 Priority claimed from US05/951,949 external-priority patent/US4206259A/en

1979-10-15 Application filed by UOP LLC filed Critical UOP LLC

1982-08-03 Application granted granted Critical

1982-08-03 Publication of CA1128917A publication Critical patent/CA1128917A/fr

Status Expired legal-status Critical Current

Links

108010093096 Immobilized Enzymes Proteins 0.000 title abstract description 19
239000011159 matrix material Substances 0.000 claims abstract description 61
239000007787 solid Substances 0.000 claims abstract description 39
239000002131 composite material Substances 0.000 claims abstract description 34
230000001588 bifunctional effect Effects 0.000 claims abstract description 32
239000000178 monomer Substances 0.000 claims abstract description 27
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
239000000463 material Substances 0.000 claims abstract description 22
239000007864 aqueous solution Substances 0.000 claims abstract description 20
238000006243 chemical reaction Methods 0.000 claims abstract description 18
229920001577 copolymer Polymers 0.000 claims abstract description 15
150000003839 salts Chemical class 0.000 claims abstract description 9
238000000151 deposition Methods 0.000 claims abstract description 7
PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical group C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 20
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 16
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 15
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical class Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 7
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 6
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
239000000919 ceramic Substances 0.000 claims description 5
229910044991 metal oxide Inorganic materials 0.000 claims description 5
238000006116 polymerization reaction Methods 0.000 claims description 5
230000000379 polymerizing effect Effects 0.000 claims description 5
238000006396 nitration reaction Methods 0.000 claims description 4
239000002685 polymerization catalyst Substances 0.000 claims description 4
USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical group [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims description 4
230000000802 nitrating effect Effects 0.000 claims description 3
229910017604 nitric acid Inorganic materials 0.000 claims description 3
JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 claims description 3
239000000377 silicon dioxide Substances 0.000 claims description 2
QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical group S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 2
238000011282 treatment Methods 0.000 claims 1
102000004190 Enzymes Human genes 0.000 abstract description 136
108090000790 Enzymes Proteins 0.000 abstract description 136
239000011148 porous material Substances 0.000 abstract description 16
238000011065 in-situ storage Methods 0.000 abstract description 4
239000011368 organic material Substances 0.000 abstract description 2
230000002301 combined effect Effects 0.000 abstract 1
229940088598 enzyme Drugs 0.000 description 135
238000000034 method Methods 0.000 description 39
239000000562 conjugate Substances 0.000 description 29
239000000203 mixture Substances 0.000 description 26
230000000694 effects Effects 0.000 description 19
239000000243 solution Substances 0.000 description 16
229960000587 glutaral Drugs 0.000 description 15
229920000642 polymer Polymers 0.000 description 15
239000002245 particle Substances 0.000 description 11
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 10
239000004365 Protease Substances 0.000 description 10
239000008103 glucose Substances 0.000 description 10
238000002360 preparation method Methods 0.000 description 9
239000000047 product Substances 0.000 description 8
229920002472 Starch Polymers 0.000 description 7
239000002253 acid Substances 0.000 description 7
238000007792 addition Methods 0.000 description 7
125000003277 amino group Chemical group 0.000 description 7
239000008107 starch Substances 0.000 description 7
235000019698 starch Nutrition 0.000 description 7
239000000758 substrate Substances 0.000 description 7
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
239000011324 bead Substances 0.000 description 6
230000003197 catalytic effect Effects 0.000 description 6
239000000839 emulsion Substances 0.000 description 6
125000000524 functional group Chemical group 0.000 description 6
238000004519 manufacturing process Methods 0.000 description 6
239000000376 reactant Substances 0.000 description 6
238000001179 sorption measurement Methods 0.000 description 6
108090000526 Papain Proteins 0.000 description 5
238000004132 cross linking Methods 0.000 description 5
239000012153 distilled water Substances 0.000 description 5
238000007429 general method Methods 0.000 description 5
230000003100 immobilizing effect Effects 0.000 description 5
229940055729 papain Drugs 0.000 description 5
235000019834 papain Nutrition 0.000 description 5
238000005406 washing Methods 0.000 description 5
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 4
102100022624 Glucoamylase Human genes 0.000 description 4
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
230000008901 benefit Effects 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
230000008878 coupling Effects 0.000 description 4
238000010168 coupling process Methods 0.000 description 4
238000005859 coupling reaction Methods 0.000 description 4
239000003431 cross linking reagent Substances 0.000 description 4
230000009849 deactivation Effects 0.000 description 4
125000001261 isocyanato group Chemical group *N=C=O 0.000 description 4
230000008569 process Effects 0.000 description 4
239000002904 solvent Substances 0.000 description 4
125000006850 spacer group Chemical group 0.000 description 4
239000004382 Amylase Substances 0.000 description 3
108010065511 Amylases Proteins 0.000 description 3
102000013142 Amylases Human genes 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
102100026189 Beta-galactosidase Human genes 0.000 description 3
108010015776 Glucose oxidase Proteins 0.000 description 3
239000004366 Glucose oxidase Substances 0.000 description 3
108091005804 Peptidases Proteins 0.000 description 3
108090000631 Trypsin Proteins 0.000 description 3
102000004142 Trypsin Human genes 0.000 description 3
108700040099 Xylose isomerases Proteins 0.000 description 3
125000002252 acyl group Chemical group 0.000 description 3
125000003172 aldehyde group Chemical group 0.000 description 3
235000019418 amylase Nutrition 0.000 description 3
108010005774 beta-Galactosidase Proteins 0.000 description 3
239000003054 catalyst Substances 0.000 description 3
239000003153 chemical reaction reagent Substances 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
239000007822 coupling agent Substances 0.000 description 3
230000008021 deposition Effects 0.000 description 3
239000000835 fiber Substances 0.000 description 3
229940116332 glucose oxidase Drugs 0.000 description 3
235000019420 glucose oxidase Nutrition 0.000 description 3
239000000543 intermediate Substances 0.000 description 3
239000007788 liquid Substances 0.000 description 3
239000012528 membrane Substances 0.000 description 3
239000008188 pellet Substances 0.000 description 3
239000002356 single layer Substances 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
239000012588 trypsin Substances 0.000 description 3
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
108090000746 Chymosin Proteins 0.000 description 2
108090000317 Chymotrypsin Proteins 0.000 description 2
229930091371 Fructose Natural products 0.000 description 2
239000005715 Fructose Substances 0.000 description 2
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
108010059881 Lactase Proteins 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
102000057297 Pepsin A Human genes 0.000 description 2
108090000284 Pepsin A Proteins 0.000 description 2
108010059712 Pronase Proteins 0.000 description 2
108010009736 Protein Hydrolysates Proteins 0.000 description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
239000006087 Silane Coupling Agent Substances 0.000 description 2
XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
150000001299 aldehydes Chemical group 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
230000004888 barrier function Effects 0.000 description 2
125000004432 carbon atom Chemical group C* 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
235000013351 cheese Nutrition 0.000 description 2
229960002376 chymotrypsin Drugs 0.000 description 2
238000001816 cooling Methods 0.000 description 2
150000002148 esters Chemical class 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
235000013305 food Nutrition 0.000 description 2
238000009472 formulation Methods 0.000 description 2
239000011521 glass Substances 0.000 description 2
229910010272 inorganic material Inorganic materials 0.000 description 2
239000011147 inorganic material Substances 0.000 description 2
229940116108 lactase Drugs 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
229940111202 pepsin Drugs 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
235000019419 proteases Nutrition 0.000 description 2
229910052710 silicon Inorganic materials 0.000 description 2
239000010703 silicon Substances 0.000 description 2
235000019333 sodium laurylsulphate Nutrition 0.000 description 2
125000003396 thiol group Chemical group [H]S* 0.000 description 2
102100033639 Acetylcholinesterase Human genes 0.000 description 1
108010022752 Acetylcholinesterase Proteins 0.000 description 1
102100036475 Alanine aminotransferase 1 Human genes 0.000 description 1
108010082126 Alanine transaminase Proteins 0.000 description 1
241000833292 Ambassis Species 0.000 description 1
108700023418 Amidases Proteins 0.000 description 1
241000370685 Arge Species 0.000 description 1
108010004032 Bromelains Proteins 0.000 description 1
102100035882 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
108010059892 Cellulase Proteins 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
108090000270 Ficain Proteins 0.000 description 1
102000005548 Hexokinase Human genes 0.000 description 1
108700040460 Hexokinases Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
108090000769 Isomerases Proteins 0.000 description 1
102000004195 Isomerases Human genes 0.000 description 1
102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
239000004952 Polyamide Substances 0.000 description 1
241000022563 Rema Species 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
108010046334 Urease Proteins 0.000 description 1
DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
150000001241 acetals Chemical class 0.000 description 1
229940022698 acetylcholinesterase Drugs 0.000 description 1
239000000654 additive Substances 0.000 description 1
235000013334 alcoholic beverage Nutrition 0.000 description 1
230000004075 alteration Effects 0.000 description 1
229940037003 alum Drugs 0.000 description 1
IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 description 1
125000000852 azido group Chemical group *N=[N+]=[N-] 0.000 description 1
108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
238000009835 boiling Methods 0.000 description 1
YZYDPPZYDIRSJT-UHFFFAOYSA-K boron phosphate Chemical compound [B+3].[O-]P([O-])([O-])=O YZYDPPZYDIRSJT-UHFFFAOYSA-K 0.000 description 1
229910000149 boron phosphate Inorganic materials 0.000 description 1
235000008429 bread Nutrition 0.000 description 1
235000019835 bromelain Nutrition 0.000 description 1
108010089934 carbohydrase Proteins 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
229940106157 cellulase Drugs 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
229920002301 cellulose acetate Polymers 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
229910010293 ceramic material Inorganic materials 0.000 description 1
239000003610 charcoal Substances 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
229940000425 combination drug Drugs 0.000 description 1
230000002596 correlated effect Effects 0.000 description 1
229920006037 cross link polymer Polymers 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
239000003599 detergent Substances 0.000 description 1
125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 1
230000001079 digestive effect Effects 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
238000004821 distillation Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
239000004744 fabric Substances 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
235000019836 ficin Nutrition 0.000 description 1
POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 1
239000010419 fine particle Substances 0.000 description 1
239000000796 flavoring agent Substances 0.000 description 1
235000019634 flavors Nutrition 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
229940042795 hydrazides for tuberculosis treatment Drugs 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000003301 hydrolyzing effect Effects 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
229910052809 inorganic oxide Inorganic materials 0.000 description 1
229910003480 inorganic solid Inorganic materials 0.000 description 1
238000009434 installation Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
239000001573 invertase Substances 0.000 description 1
235000011073 invertase Nutrition 0.000 description 1
238000006317 isomerization reaction Methods 0.000 description 1
NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
229910052622 kaolinite Inorganic materials 0.000 description 1
239000010410 layer Substances 0.000 description 1
239000010985 leather Substances 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
238000005007 materials handling Methods 0.000 description 1
235000013372 meat Nutrition 0.000 description 1
150000004706 metal oxides Chemical class 0.000 description 1
239000010445 mica Substances 0.000 description 1
229910052618 mica group Inorganic materials 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
239000012299 nitrogen atmosphere Substances 0.000 description 1
239000002674 ointment Substances 0.000 description 1
229920000620 organic polymer Polymers 0.000 description 1
238000006053 organic reaction Methods 0.000 description 1
150000001282 organosilanes Chemical class 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
150000003016 phosphoric acids Chemical class 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920002647 polyamide Polymers 0.000 description 1
239000000843 powder Substances 0.000 description 1
230000002265 prevention Effects 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
239000012429 reaction media Substances 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000000452 restraining effect Effects 0.000 description 1
239000004576 sand Substances 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000013341 scale-up Methods 0.000 description 1
229920005573 silicon-containing polymer Polymers 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
-1 spacer compound Chemical class 0.000 description 1
230000003335 steric effect Effects 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
229920003176 water-insoluble polymer Polymers 0.000 description 1
239000010457 zeolite Substances 0.000 description 1
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
- C08F8/04—Reduction, e.g. hydrogenation

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Inorganic Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
Polymers & Plastics (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

CA337,572A 1978-10-16 1979-10-15 Matrice de support pour enzymes immobilises Expired CA1128917A (fr)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US951,949		1978-10-16
US05/951,948 US4193910A (en)	1978-10-16	1978-10-16	Preparation of support matrices for immobilized enzymes
US951,948		1978-10-16
US05/951,949 US4206259A (en)	1978-10-16	1978-10-16	Support matrices for immobilized enzymes

Publications (1)

Publication Number	Publication Date
CA1128917A true CA1128917A (fr)	1982-08-03

Family

ID=27130323

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA337,572A Expired CA1128917A (fr)	1978-10-16	1979-10-15	Matrice de support pour enzymes immobilises

Country Status (7)

Country	Link
CA (1)	CA1128917A (fr)
DE (1)	DE2941881C2 (fr)
DK (1)	DK436379A (fr)
ES (1)	ES485047A1 (fr)
FR (1)	FR2453898A1 (fr)
GB (1)	GB2033399B (fr)
IT (1)	IT1125483B (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
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EP0062968B2 (fr) *	1981-03-18	1991-07-10	FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC.	Matérial de support pour l'utilisation dans l'essai sérologique et sa préparation
DE3130924A1 (de) *	1981-08-05	1983-02-17	Röhm GmbH, 6100 Darmstadt	Oberflaechenreiche systeme zur fixierung von nucleophile gruppen enthaltenden substraten
CN113061600A (zh) *	2021-03-05	2021-07-02	宁波泓森生物科技有限公司	一种固定化苏氨酸醛缩酶的制备方法、固定化苏氨酸醛缩酶及应用
CN114540332B (zh) *	2022-03-01	2023-07-21	江苏恰瑞生物科技有限公司	一种尿酸酶固定化树脂的制备方法及其应用

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* Cited by examiner, † Cited by third party
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US3860486A (en) *	1973-05-24	1975-01-14	Owens Illinois Inc	Insolubilizing enzymes with polystyrene derivatives
GB1485122A (en) *	1973-08-06	1977-09-08	Nat Res Dev	Biologically active matrices
US4001085A (en) *	1973-09-10	1977-01-04	Owens-Illinois, Inc.	Immobilization of enzymes on an inorganic matrix
GB1485123A (en) *	1974-07-25	1977-09-08	Nat Res Dev	Support for biologically active material
PT64790B (en) *	1975-02-18	1977-07-07	Uop Inc	Immobilized enzyme conjugates
US4141857A (en) *	1976-04-30	1979-02-27	Uop Inc.	Support matrices for immobilized enzymes

1979
- 1979-10-15 CA CA337,572A patent/CA1128917A/fr not_active Expired
- 1979-10-16 DK DK436379A patent/DK436379A/da not_active Application Discontinuation
- 1979-10-16 IT IT26535/79A patent/IT1125483B/it active
- 1979-10-16 FR FR7925672A patent/FR2453898A1/fr active Granted
- 1979-10-16 GB GB7935953A patent/GB2033399B/en not_active Expired
- 1979-10-16 DE DE2941881A patent/DE2941881C2/de not_active Expired
- 1979-10-16 ES ES485047A patent/ES485047A1/es not_active Expired

Also Published As

Publication number	Publication date
IT7926535A0 (it)	1979-10-16
DE2941881A1 (de)	1980-04-17
IT1125483B (it)	1986-05-14
DK436379A (da)	1980-04-17
FR2453898B1 (fr)	1983-11-18
FR2453898A1 (fr)	1980-11-07
ES485047A1 (es)	1980-05-16
GB2033399A (en)	1980-05-21
DE2941881C2 (de)	1983-11-10
GB2033399B (en)	1982-11-24

Legal Events

Date	Code	Title	Description
1999-08-03	MKEX	Expiry

Publication	Publication Date	Title
US4141857A (en)	1979-02-27	Support matrices for immobilized enzymes
US4229536A (en)	1980-10-21	Process for preparing immobilized enzymes
US4102746A (en)	1978-07-25	Immobilized proteins
US4169014A (en)	1979-09-25	Method of immobilizing proteinaceous substances
EP0216272B1 (fr)	1993-05-26	Immobilisation de biocatalyseurs sur de la terre de diatomées granuleuse
EP0104571B1 (fr)	1989-03-22	Immobilisation de biocatalyseurs sur carbone granulé
US4888285A (en)	1989-12-19	Enzyme immobilization on a water-insoluble amino group-containing carrier
US4193910A (en)	1980-03-18	Preparation of support matrices for immobilized enzymes
US4206259A (en)	1980-06-03	Support matrices for immobilized enzymes
US4218363A (en)	1980-08-19	Preparation of support matrices for immobilized enzymes
US3849253A (en)	1974-11-19	Process of immobilizing enzymes
CA1128917A (fr)	1982-08-03	Matrice de support pour enzymes immobilises
US4250080A (en)	1981-02-10	Preparation of support matrices for immobilized enzymes
Suckling	1977	Immobilized enzymes
IE42482B1 (en)	1980-08-13	Immobilized enzyme conjugates
US4416992A (en)	1983-11-22	Support matrices and immobilized enzyme systems
Powell	1984	Developments in immobilized-enzyme technology
KR100338566B1 (ko)	2002-10-31	기질상에고정된페니실린g아미다아제,글루타릴-7-aca아실라아제또는d-아미노산옥시다아제
JP2781990B2 (ja)	1998-07-30	固定化酵素の製造法
JPH022587B2 (fr)	1990-01-18
JPS62118889A (ja)	1987-05-30	生物学的物質を固定化する方法及びこの方法によつて得られる固定化生物学的物質
Epton et al.	1976	[7] Enzymes covalently bound to polyacrylic and polymethacrylic copolymers
JPS61181376A (ja)	1986-08-14	固定化された生物学的活性化合物の製造方法
SU859372A1 (ru)	1981-08-30	Способ получени активированных носителей
Zaborsky	1977	Immobilization of enzymes by adsorption