CA1129806A - Appareil et procede de microelectrophorese - Google Patents

Appareil et procede de microelectrophorese

Info

Publication number: CA1129806A
Authority: CA; Canada
Prior art keywords: gel; membrane; protein; tray; group
Prior art date: 1977-11-10
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA370,920A

Other languages

English (en)

Inventor

Benjamin W. Grunbaum

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

National Aeronautics and Space Administration NASA

Original Assignee

National Aeronautics and Space Administration NASA

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1977-11-10

Filing date

1981-02-13

Publication date

1982-08-17

1977-11-10 Priority claimed from US05/850,507 external-priority patent/US4130471A/en

1981-02-13 Application filed by National Aeronautics and Space Administration NASA filed Critical National Aeronautics and Space Administration NASA

1981-02-13 Priority to CA370,920A priority Critical patent/CA1129806A/fr

1982-08-17 Application granted granted Critical

1982-08-17 Publication of CA1129806A publication Critical patent/CA1129806A/fr

Status Expired legal-status Critical Current

Links

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239000012528 membrane Substances 0.000 claims abstract description 78
102000004169 proteins and genes Human genes 0.000 claims abstract description 43
108090000623 proteins and genes Proteins 0.000 claims abstract description 43
239000000758 substrate Substances 0.000 claims abstract description 17
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
108020004774 Alkaline Phosphatase Proteins 0.000 claims abstract description 13
102000002260 Alkaline Phosphatase Human genes 0.000 claims abstract description 13
102000004420 Creatine Kinase Human genes 0.000 claims abstract description 13
108010042126 Creatine kinase Proteins 0.000 claims abstract description 13
229920002301 cellulose acetate Polymers 0.000 claims abstract description 12
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
238000000926 separation method Methods 0.000 claims abstract description 11
101710169336 5'-deoxyadenosine deaminase Proteins 0.000 claims abstract description 9
102100031126 6-phosphogluconolactonase Human genes 0.000 claims abstract description 9
108010029731 6-phosphogluconolactonase Proteins 0.000 claims abstract description 9
102000055025 Adenosine deaminases Human genes 0.000 claims abstract description 9
102000002281 Adenylate kinase Human genes 0.000 claims abstract description 9
108020000543 Adenylate kinase Proteins 0.000 claims abstract description 9
108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims abstract description 9
102000028528 s-formylglutathione hydrolase Human genes 0.000 claims abstract description 9
108010093322 s-formylglutathione hydrolase Proteins 0.000 claims abstract description 9
102100036475 Alanine aminotransferase 1 Human genes 0.000 claims abstract description 8
108010082126 Alanine transaminase Proteins 0.000 claims abstract description 8
235000014655 lactic acid Nutrition 0.000 claims abstract description 7
239000004310 lactic acid Substances 0.000 claims abstract description 7
101710088194 Dehydrogenase Proteins 0.000 claims abstract description 6
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims abstract description 5
108010051457 Acid Phosphatase Proteins 0.000 claims abstract description 5
102000013563 Acid Phosphatase Human genes 0.000 claims abstract description 5
102000004895 Lipoproteins Human genes 0.000 claims abstract description 5
108090001030 Lipoproteins Proteins 0.000 claims abstract description 5
210000003743 erythrocyte Anatomy 0.000 claims abstract description 5
102000004567 6-phosphogluconate dehydrogenase Human genes 0.000 claims abstract description 4
108020001657 6-phosphogluconate dehydrogenase Proteins 0.000 claims abstract description 4
101100268493 Cunninghamella elegans 6-PGD gene Proteins 0.000 claims abstract description 4
238000004519 manufacturing process Methods 0.000 claims abstract description 4
102100038611 Vitamin D-binding protein Human genes 0.000 claims abstract description 3
101710179590 Vitamin D-binding protein Proteins 0.000 claims abstract description 3
238000001962 electrophoresis Methods 0.000 claims description 19
102000004190 Enzymes Human genes 0.000 claims description 9
108090000790 Enzymes Proteins 0.000 claims description 9
229920000728 polyester Polymers 0.000 claims description 6
108010017384 Blood Proteins Proteins 0.000 claims description 3
102000004506 Blood Proteins Human genes 0.000 claims description 3
108010054147 Hemoglobins Proteins 0.000 claims description 3
102000001554 Hemoglobins Human genes 0.000 claims description 3
101710100170 Unknown protein Proteins 0.000 claims description 2
239000002131 composite material Substances 0.000 claims description 2
238000001035 drying Methods 0.000 claims description 2
102000009569 Phosphoglucomutase Human genes 0.000 claims 1
108091000115 phosphomannomutase Proteins 0.000 claims 1
239000000499 gel Substances 0.000 description 76
210000004369 blood Anatomy 0.000 description 11
239000008280 blood Substances 0.000 description 11
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HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
108050005077 Haptoglobin Proteins 0.000 description 1
102000014702 Haptoglobin Human genes 0.000 description 1
208000037319 Hepatitis infectious Diseases 0.000 description 1
108060003951 Immunoglobulin Proteins 0.000 description 1
108010044467 Isoenzymes Proteins 0.000 description 1
101100459301 Mus musculus Myl4 gene Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
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239000004952 Polyamide Substances 0.000 description 1
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229910052751 metal Inorganic materials 0.000 description 1
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YLGXILFCIXHCMC-JHGZEJCSSA-N methyl cellulose Chemical compound COC1C(OC)C(OC)C(COC)O[C@H]1O[C@H]1C(OC)C(OC)C(OC)OC1COC YLGXILFCIXHCMC-JHGZEJCSSA-N 0.000 description 1
239000000203 mixture Substances 0.000 description 1
239000000615 nonconductor Substances 0.000 description 1
239000012188 paraffin wax Substances 0.000 description 1
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 1
229920002647 polyamide Polymers 0.000 description 1
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230000000630 rising effect Effects 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000007789 sealing Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
238000003860 storage Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
208000006379 syphilis Diseases 0.000 description 1
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239000002470 thermal conductor Substances 0.000 description 1
210000002105 tongue Anatomy 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA370,920A 1977-11-10 1981-02-13 Appareil et procede de microelectrophorese Expired CA1129806A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA370,920A CA1129806A (fr)	1977-11-10	1981-02-13	Appareil et procede de microelectrophorese

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US05/850,507 US4130471A (en)	1977-11-10	1977-11-10	Microelectrophoretic apparatus and process
US850,507		1977-11-10
CA314,426A CA1116062A (fr)	1977-11-10	1978-10-26	Appareil microelectrophoretique et methode
CA370,920A CA1129806A (fr)	1977-11-10	1981-02-13	Appareil et procede de microelectrophorese

Publications (1)

Publication Number	Publication Date
CA1129806A true CA1129806A (fr)	1982-08-17

Family

ID=27165942

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA370,920A Expired CA1129806A (fr)	1977-11-10	1981-02-13	Appareil et procede de microelectrophorese

Country Status (1)

Country	Link
CA (1)	CA1129806A (fr)

1981
- 1981-02-13 CA CA370,920A patent/CA1129806A/fr not_active Expired

Legal Events

Date	Code	Title	Description
1999-08-17	MKEX	Expiry

Publication	Publication Date	Title
CA1116062A (fr)	1982-01-12	Appareil microelectrophoretique et methode
US4415418A (en)	1983-11-15	Gel electrophoresis device and method
CA1085772A (fr)	1980-09-16	Appareil pour effectuer des separations par electrophorese
Chrambach et al.	1976	Analytical and preparative polyacrylamide gel electrophoresis: An objectively defined fractionation route, apparatus, and procedures
EP0457526B1 (fr)	1999-03-03	Système pour électrophorèse à gel
US3674678A (en)	1972-07-04	Electrophoretic apparatus
US3736100A (en)	1973-05-29	Immunoelectrophoresis gel tray
Poehling et al.	1980	One‐and two‐dimensional electrophoresis in micro‐slab gels
US20070284250A1 (en)	2007-12-13	Multifunctional Electrophoresis Cassette
US4018662A (en)	1977-04-19	Method and apparatus for simultaneous quantitative analysis of several constituents in a sample
US2843540A (en)	1958-07-15	Method of electrophoresis of serum proteins
US3930973A (en)	1976-01-06	Electrophoretic process
JP3093929B2 (ja)	2000-10-03	電気泳動ゲルプレートならびに電気泳動ゲルプレートパッケージおよびそれを作る方法
US3879280A (en)	1975-04-22	Gel slab electrophoresis cell and electrophoresis apparatus utilizing same
US3773646A (en)	1973-11-20	Electrophoresis test kits
Catsimpoolas	1970	Isoelectric focusing of proteins in gel media
US20050072678A1 (en)	2005-04-07	Apparatus for electrophoresis
US4094759A (en)	1978-06-13	Method for simultaneous quantitative analysis of several constituents in a sample
US3699033A (en)	1972-10-17	Electrophoresis and electrofocusing
CA1129806A (fr)	1982-08-17	Appareil et procede de microelectrophorese
US3988230A (en)	1976-10-26	Chamber and process for crossed immunoelectro-phoresis
CA1124681A (fr)	1982-06-01	Appareil et methode de microelectrophorese
US6632340B2 (en)	2003-10-14	Precast gel and tray combination for submerged gel electrophoresis
Grunbaum	1975	An automatic one-to eight-sample applicator for fast qualitative and quantitative microelectrophoresis of plasma proteins, hemoglobins, isoenzymes, and cross-over electrophoresis
Kjellin et al.	1979	Isotachophoresis of CSF proteins in gel tubes especially gammaglobulins: An analytical and preparative technique for high-separation of CSF proteins