CA1142513A - Methode immuno-radiologique pour le dosage de la thymosine alpha - 1 - Google Patents

Methode immuno-radiologique pour le dosage de la thymosine alpha - 1

Info

Publication number: CA1142513A
Authority: CA; Canada
Prior art keywords: thymosin; serum; alpha; immunogen; diseases
Prior art date: 1979-01-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000409068A

Other languages

English (en)

Inventor

Allan L. Goldstein

John E. Mcclure

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

George Washington University

Original Assignee

George Washington University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1979-01-22

Filing date

1982-08-09

Publication date

1983-03-08

1979-01-22 Priority claimed from US06/004,971 external-priority patent/US4264571A/en

1982-08-09 Application filed by George Washington University filed Critical George Washington University

1982-08-09 Priority to CA000409068A priority Critical patent/CA1142513A/fr

1983-03-08 Application granted granted Critical

1983-03-08 Publication of CA1142513A publication Critical patent/CA1142513A/fr

Status Expired legal-status Critical Current

Links

NZVYCXVTEHPMHE-ZSUJOUNUSA-N thymalfasin Chemical compound CC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O NZVYCXVTEHPMHE-ZSUJOUNUSA-N 0.000 title claims abstract 6
238000003127 radioimmunoassay Methods 0.000 title description 8
108010078233 Thymalfasin Proteins 0.000 title 1
102400000800 Thymosin alpha-1 Human genes 0.000 title 1
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SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 4
239000012876 carrier material Substances 0.000 claims description 4
108060003552 hemocyanin Proteins 0.000 claims description 4
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230000001588 bifunctional effect Effects 0.000 claims description 3
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108010046075 Thymosin Proteins 0.000 description 60
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LCJVIYPJPCBWKS-NXPQJCNCSA-N thymosin Chemical compound SC[C@@H](N)C(=O)N[C@H](CO)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CO)C(=O)N[C@H](CO)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@H]([C@H](C)O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@H](CCC(O)=O)C(O)=O LCJVIYPJPCBWKS-NXPQJCNCSA-N 0.000 description 60
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102100023981 Lamina-associated polypeptide 2, isoform alpha Human genes 0.000 description 5
101710163560 Lamina-associated polypeptide 2, isoform alpha Proteins 0.000 description 5
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150000001413 amino acids Chemical class 0.000 description 5
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FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
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RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 3
KYRUKRFVOACELK-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(4-hydroxyphenyl)propanoate Chemical compound C1=CC(O)=CC=C1CCC(=O)ON1C(=O)CCC1=O KYRUKRFVOACELK-UHFFFAOYSA-N 0.000 description 2
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LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
241001494479 Pecora Species 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
230000002378 acidificating effect Effects 0.000 description 2
239000002671 adjuvant Substances 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
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230000003053 immunization Effects 0.000 description 2
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150000002500 ions Chemical class 0.000 description 2
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125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
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239000006228 supernatant Substances 0.000 description 2
108700016958 thymosin fraction 5 Proteins 0.000 description 2
210000001541 thymus gland Anatomy 0.000 description 2
WFIYPADYPQQLNN-UHFFFAOYSA-N 2-[2-(4-bromopyrazol-1-yl)ethyl]isoindole-1,3-dione Chemical compound C1=C(Br)C=NN1CCN1C(=O)C2=CC=CC=C2C1=O WFIYPADYPQQLNN-UHFFFAOYSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
108010017384 Blood Proteins Proteins 0.000 description 1
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OKTJSMMVPCPJKN-NJFSPNSNSA-N Carbon-14 Chemical compound [14C] OKTJSMMVPCPJKN-NJFSPNSNSA-N 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
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238000004435 EPR spectroscopy Methods 0.000 description 1
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241000283086 Equidae Species 0.000 description 1
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239000004471 Glycine Substances 0.000 description 1
108091006905 Human Serum Albumin Proteins 0.000 description 1
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DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
241001049988 Mycobacterium tuberculosis H37Ra Species 0.000 description 1
AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
102000015731 Peptide Hormones Human genes 0.000 description 1
108010038988 Peptide Hormones Proteins 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
108010039918 Polylysine Proteins 0.000 description 1
241000700159 Rattus Species 0.000 description 1
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QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
210000001744 T-lymphocyte Anatomy 0.000 description 1
241000473945 Theria <moth genus> Species 0.000 description 1
YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
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VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 1
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LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
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125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
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WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
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XMBWDFGMSWQBCA-YPZZEJLDSA-N iodane Chemical compound [125IH] XMBWDFGMSWQBCA-YPZZEJLDSA-N 0.000 description 1
229940044173 iodine-125 Drugs 0.000 description 1
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208000024891 symptom Diseases 0.000 description 1
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230000009466 transformation Effects 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
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201000008827 tuberculosis Diseases 0.000 description 1
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125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
239000011800 void material Substances 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1

Landscapes

Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

CA000409068A 1979-01-22 1982-08-09 Methode immuno-radiologique pour le dosage de la thymosine alpha - 1 Expired CA1142513A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000409068A CA1142513A (fr)	1979-01-22	1982-08-09	Methode immuno-radiologique pour le dosage de la thymosine alpha - 1

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US4,971		1979-01-22
US06/004,971 US4264571A (en)	1979-01-22	1979-01-22	Radioimmunoassay of thymosin α1
CA000343285A CA1138437A (fr)	1979-01-22	1980-01-08	Dosage radioimmunologique de la thymosine alpha.sub.1
CA000409068A CA1142513A (fr)	1979-01-22	1982-08-09	Methode immuno-radiologique pour le dosage de la thymosine alpha - 1

Publications (1)

Publication Number	Publication Date
CA1142513A true CA1142513A (fr)	1983-03-08

Family

ID=27166538

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000409068A Expired CA1142513A (fr)	1979-01-22	1982-08-09	Methode immuno-radiologique pour le dosage de la thymosine alpha - 1

Country Status (1)

Country	Link
CA (1)	CA1142513A (fr)

1982
- 1982-08-09 CA CA000409068A patent/CA1142513A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2000-03-08	MKEX	Expiry

Publication	Publication Date	Title
CA1138437A (fr)	1982-12-28	Dosage radioimmunologique de la thymosine alpha.sub.1
US4478744A (en)	1984-10-23	Method of obtaining antibodies
US3766162A (en)	1973-10-16	Barbituric acid antigens and antibodies specific therefor
US4312853A (en)	1982-01-26	Radioimmunological determination of procollagen (type III) and procollagen peptide (type III)
EP0111211B1 (fr)	1986-05-21	Immunoessai pour protéines et fragments de protéines non enzymatiquement glucosylés - un indice de la glycémie
Dorrington et al.	1966	The long‐acting thyroid stimulator
US4621048A (en)	1986-11-04	Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy
US4339427A (en)	1982-07-13	Radioimmunoassay of thymosinα
Mercham et al.	1982	[42] Antibodies to insoluble and solubilized elastin
JPH0428718B2 (fr)	1992-05-15
US4124700A (en)	1978-11-07	Immunoassay for thymopoietin
CA1194793A (fr)	1985-10-08	Production d'antigenes fucosyl et d'anticorps correspondants, determination de liaisons associees au cancer dans les hydrates de carbone au moyen des dits antigenes-anticorps et rrousse de diagnostic
EP0020090B1 (fr)	1984-10-10	Radio-immunoessai pour facteur inhibiteur de migration
Salabe et al.	1972	Radioimmunoassay for Human Antithyroglobulin Antibodies I. The Relationship Between Tanned Cell Haemagglutination and a Double Antibody Technique
Goldstein	1976	Radioimmunoassay for thymopoietin
FI95579C (fi)	1996-02-26	Immunologinen menetelmä intaktin prokollageenipeptidin (tyyppi III) ja prokollageenin (tyyppi III) määrittämiseksi selektiivisesti elimistönesteistä ja väline sen toteuttamiseksi
US4543340A (en)	1985-09-24	Radioimmunoassay of thymosin β4
Grey et al.	1970	The subunit structure of mouse IgA
US4096237A (en)	1978-06-20	Immunoassay for β-endorphin
CA1142513A (fr)	1983-03-08	Methode immuno-radiologique pour le dosage de la thymosine alpha - 1
US4151268A (en)	1979-04-24	Barbituric acid antigens and antibodies specific therefor
CA1146938A (fr)	1983-05-24	Dosage radioimmunologique de la thymosine alpha 1
CA1075600A (fr)	1980-04-15	Dosage immunologique de la thymopoietine
Newton et al.	1970	Radioimmunoassay of peptides lacking tyrosine
Goldstein et al.	1983	Radioimmunoassay of thymosin alpha 1