CA1156579A - Regeneration d'un systeme enzymatique immobilise - Google Patents

Regeneration d'un systeme enzymatique immobilise

Info

Publication number: CA1156579A
Authority: CA; Canada
Prior art keywords: enzyme; acid; group; immobilized; binding layer
Prior art date: 1981-01-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000368348A

Other languages

English (en)

Inventor

Joseph Levy

Ronald P. Rohrbach

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Honeywell UOP LLC

Original Assignee

UOP LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-01-13

Filing date

1981-01-13

Publication date

1983-11-08

1981-01-13 Application filed by UOP LLC filed Critical UOP LLC

1981-01-13 Priority to CA000368348A priority Critical patent/CA1156579A/fr

1983-11-08 Application granted granted Critical

1983-11-08 Publication of CA1156579A publication Critical patent/CA1156579A/fr

Status Expired legal-status Critical Current

Links

108010093096 Immobilized Enzymes Proteins 0.000 title claims abstract description 25
238000011069 regeneration method Methods 0.000 title abstract description 9
230000008929 regeneration Effects 0.000 title abstract description 6
102000004190 Enzymes Human genes 0.000 claims abstract description 61
108090000790 Enzymes Proteins 0.000 claims abstract description 61
238000000034 method Methods 0.000 claims abstract description 28
239000002253 acid Substances 0.000 claims abstract description 16
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 16
230000001588 bifunctional effect Effects 0.000 claims abstract description 7
230000003100 immobilizing effect Effects 0.000 claims abstract description 4
230000001172 regenerating effect Effects 0.000 claims abstract description 4
229940088598 enzyme Drugs 0.000 claims description 60
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 18
239000000463 material Substances 0.000 claims description 13
230000008569 process Effects 0.000 claims description 13
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
239000003795 chemical substances by application Substances 0.000 claims description 8
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 5
125000000524 functional group Chemical group 0.000 claims description 5
229920000768 polyamine Polymers 0.000 claims description 5
102100022624 Glucoamylase Human genes 0.000 claims description 4
AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 claims description 4
229920002873 Polyethylenimine Polymers 0.000 claims description 4
NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 claims description 4
JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 claims description 4
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
PCSMJKASWLYICJ-UHFFFAOYSA-N Succinic aldehyde Chemical compound O=CCCC=O PCSMJKASWLYICJ-UHFFFAOYSA-N 0.000 claims description 3
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 3
229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
239000003431 cross linking reagent Substances 0.000 claims description 3
239000011521 glass Substances 0.000 claims description 3
239000008103 glucose Substances 0.000 claims description 3
DVKJHBMWWAPEIU-UHFFFAOYSA-N toluene 2,4-diisocyanate Chemical compound CC1=CC=C(N=C=O)C=C1N=C=O DVKJHBMWWAPEIU-UHFFFAOYSA-N 0.000 claims description 3
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims description 2
VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims description 2
108010059892 Cellulase Proteins 0.000 claims description 2
RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 claims description 2
PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 2
SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 claims description 2
229940092714 benzenesulfonic acid Drugs 0.000 claims description 2
229940106157 cellulase Drugs 0.000 claims description 2
229940098779 methanesulfonic acid Drugs 0.000 claims description 2
LSHROXHEILXKHM-UHFFFAOYSA-N n'-[2-[2-[2-(2-aminoethylamino)ethylamino]ethylamino]ethyl]ethane-1,2-diamine Chemical compound NCCNCCNCCNCCNCCN LSHROXHEILXKHM-UHFFFAOYSA-N 0.000 claims description 2
FAGUFWYHJQFNRV-UHFFFAOYSA-N tetraethylenepentamine Chemical compound NCCNCCNCCNCCN FAGUFWYHJQFNRV-UHFFFAOYSA-N 0.000 claims description 2
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 2
239000011159 matrix material Substances 0.000 claims 4
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 2
BMYNFMYTOJXKLE-UHFFFAOYSA-N 3-azaniumyl-2-hydroxypropanoate Chemical compound NCC(O)C(O)=O BMYNFMYTOJXKLE-UHFFFAOYSA-N 0.000 claims 1
102000004195 Isomerases Human genes 0.000 claims 1
108090000769 Isomerases Proteins 0.000 claims 1
229910010293 ceramic material Inorganic materials 0.000 claims 1
TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims 1
229910052814 silicon oxide Inorganic materials 0.000 claims 1
229960004319 trichloroacetic acid Drugs 0.000 claims 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
230000000694 effects Effects 0.000 description 9
239000000243 solution Substances 0.000 description 8
108700040099 Xylose isomerases Proteins 0.000 description 7
-1 alumina Chemical class 0.000 description 6
239000000126 substance Substances 0.000 description 6
150000007513 acids Chemical class 0.000 description 5
150000005829 chemical entities Chemical class 0.000 description 5
238000010908 decantation Methods 0.000 description 5
239000008188 pellet Substances 0.000 description 5
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 4
239000007864 aqueous solution Substances 0.000 description 4
230000002255 enzymatic effect Effects 0.000 description 4
239000007788 liquid Substances 0.000 description 4
125000005442 diisocyanate group Chemical group 0.000 description 3
230000007246 mechanism Effects 0.000 description 3
229910044991 metal oxide Inorganic materials 0.000 description 3
150000004706 metal oxides Chemical class 0.000 description 3
239000007787 solid Substances 0.000 description 3
238000005406 washing Methods 0.000 description 3
239000004365 Protease Substances 0.000 description 2
238000013019 agitation Methods 0.000 description 2
238000013459 approach Methods 0.000 description 2
238000001311 chemical methods and process Methods 0.000 description 2
238000003776 cleavage reaction Methods 0.000 description 2
230000009849 deactivation Effects 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
239000011148 porous material Substances 0.000 description 2
230000007420 reactivation Effects 0.000 description 2
239000011347 resin Substances 0.000 description 2
229920005989 resin Polymers 0.000 description 2
230000007017 scission Effects 0.000 description 2
238000003756 stirring Methods 0.000 description 2
108700023418 Amidases Proteins 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
108010065511 Amylases Proteins 0.000 description 1
102000013142 Amylases Human genes 0.000 description 1
102100026189 Beta-galactosidase Human genes 0.000 description 1
108090000317 Chymotrypsin Proteins 0.000 description 1
108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
240000002989 Euphorbia neriifolia Species 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
102000005548 Hexokinase Human genes 0.000 description 1
108700040460 Hexokinases Proteins 0.000 description 1
108010059881 Lactase Proteins 0.000 description 1
108090000526 Papain Proteins 0.000 description 1
102000057297 Pepsin A Human genes 0.000 description 1
108090000284 Pepsin A Proteins 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
238000002679 ablation Methods 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000008351 acetate buffer Substances 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
125000003172 aldehyde group Chemical group 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
235000019418 amylase Nutrition 0.000 description 1
238000003556 assay Methods 0.000 description 1
108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
239000000919 ceramic Substances 0.000 description 1
238000007385 chemical modification Methods 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
229960002376 chymotrypsin Drugs 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
239000013505 freshwater Substances 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
239000012535 impurity Substances 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
239000001573 invertase Substances 0.000 description 1
235000011073 invertase Nutrition 0.000 description 1
229940116108 lactase Drugs 0.000 description 1
230000007774 longterm Effects 0.000 description 1
239000002184 metal Substances 0.000 description 1
239000011707 mineral Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
239000011368 organic material Substances 0.000 description 1
229920000620 organic polymer Polymers 0.000 description 1
229940055729 papain Drugs 0.000 description 1
235000019834 papain Nutrition 0.000 description 1
229940111202 pepsin Drugs 0.000 description 1
231100000572 poisoning Toxicity 0.000 description 1
230000000607 poisoning effect Effects 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
KCXFHTAICRTXLI-UHFFFAOYSA-N propane-1-sulfonic acid Chemical compound CCCS(O)(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-N 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000003716 rejuvenation Effects 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
KUCOHFSKRZZVRO-UHFFFAOYSA-N terephthalaldehyde Chemical compound O=CC1=CC=C(C=O)C=C1 KUCOHFSKRZZVRO-UHFFFAOYSA-N 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000000844 transformation Methods 0.000 description 1
229960001124 trientine Drugs 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
229960001322 trypsin Drugs 0.000 description 1

Landscapes

Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

CA000368348A 1981-01-13 1981-01-13 Regeneration d'un systeme enzymatique immobilise Expired CA1156579A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000368348A CA1156579A (fr)	1981-01-13	1981-01-13	Regeneration d'un systeme enzymatique immobilise

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000368348A CA1156579A (fr)	1981-01-13	1981-01-13	Regeneration d'un systeme enzymatique immobilise

Publications (1)

Publication Number	Publication Date
CA1156579A true CA1156579A (fr)	1983-11-08

Family

ID=4118897

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000368348A Expired CA1156579A (fr)	1981-01-13	1981-01-13	Regeneration d'un systeme enzymatique immobilise

Country Status (1)

Country	Link
CA (1)	CA1156579A (fr)

1981
- 1981-01-13 CA CA000368348A patent/CA1156579A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2000-11-08	MKEX	Expiry

Publication	Publication Date	Title
US4141857A (en)	1979-02-27	Support matrices for immobilized enzymes
US4581336A (en)	1986-04-08	Surface-modified electrodes
EP0104571B1 (fr)	1989-03-22	Immobilisation de biocatalyseurs sur carbone granulé
US4343901A (en)	1982-08-10	Magnetic support matrix for enzyme immobilization
US4713333A (en)	1987-12-15	Immobilization of biocatalysts on granular diatomaceous earth
CA2131225A1 (fr)	1995-03-02	Methode pour preparer un conjugue d'enzyme immobilise; conjugues d'enzymes immobilises ainsi obtenus
US4268423A (en)	1981-05-19	Support matrices for immobilized enzymes
US4248969A (en)	1981-02-03	Regeneration of a immobilized enzyme system
US4268419A (en)	1981-05-19	Support matrices for immobilized enzymes
US4292199A (en)	1981-09-29	Method of preparing a support matrix
US4250260A (en)	1981-02-10	Regeneration of an immobilized enzyme system
US4072566A (en)	1978-02-07	Immobilized biologically active proteins
US4206259A (en)	1980-06-03	Support matrices for immobilized enzymes
US4193910A (en)	1980-03-18	Preparation of support matrices for immobilized enzymes
CA1156579A (fr)	1983-11-08	Regeneration d'un systeme enzymatique immobilise
US4416992A (en)	1983-11-22	Support matrices and immobilized enzyme systems
US4337172A (en)	1982-06-29	Enhanced immobilization of a glucose isomerase
EP0055788B1 (fr)	1986-04-16	Méthode de régénération d'un système d'enzymes immobilisés
US4218363A (en)	1980-08-19	Preparation of support matrices for immobilized enzymes
CS209424B2 (en)	1981-12-31	Method of making the anorganic-organic base for immobilization of enzymes
US4525456A (en)	1985-06-25	Support matrix and immobilized enzyme system
US4279998A (en)	1981-07-21	Regenerable insoluble support for protein immobilization
FI101400B (fi)	1998-06-15	Menetelmä kantajaan sidottujen entsyymien valmistamiseksi
CA1156580A (fr)	1983-11-08	Regeneration d'un systeme enzymatique immobilise
KR860001229B1 (ko)	1986-08-30	고정화 효소계의 재생방법