CA1167747A - Solution enzymatique pour le dosage de l'uree - Google Patents

Solution enzymatique pour le dosage de l'uree

Info

Publication number: CA1167747A
Authority: CA; Canada
Prior art keywords: solution; urease; stabilized; amount; recited
Prior art date: 1980-12-03
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000366092A

Other languages

English (en)

Inventor

Ivan E. Modrovich

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Individual

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-12-03

Filing date

1980-12-03

Publication date

1984-05-22

1980-12-03 Application filed by Individual filed Critical Individual

1980-12-03 Priority to CA000366092A priority Critical patent/CA1167747A/fr

1984-05-22 Application granted granted Critical

1984-05-22 Publication of CA1167747A publication Critical patent/CA1167747A/fr

Status Expired legal-status Critical Current

Links

239000004202 carbamide Substances 0.000 title description 45
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 title description 44
239000000243 solution Substances 0.000 claims abstract description 152
108010046334 Urease Proteins 0.000 claims abstract description 106
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
229910001868 water Inorganic materials 0.000 claims abstract description 35
239000000203 mixture Substances 0.000 claims abstract description 22
150000002894 organic compounds Chemical class 0.000 claims abstract description 17
GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 16
239000006172 buffering agent Substances 0.000 claims abstract description 11
239000002738 chelating agent Substances 0.000 claims abstract description 9
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 5
CBTVGIZVANVGBH-UHFFFAOYSA-N aminomethyl propanol Chemical compound CC(C)(N)CO CBTVGIZVANVGBH-UHFFFAOYSA-N 0.000 claims abstract description 4
ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 claims abstract description 4
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 57
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 36
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 36
HFZWRUODUSTPEG-UHFFFAOYSA-N 2,4-dichlorophenol Chemical group OC1=CC=C(Cl)C=C1Cl HFZWRUODUSTPEG-UHFFFAOYSA-N 0.000 claims description 23
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 19
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 15
230000000694 effects Effects 0.000 claims description 14
239000003085 diluting agent Substances 0.000 claims description 13
FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 12
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 12
239000000600 sorbitol Substances 0.000 claims description 12
229960001484 edetic acid Drugs 0.000 claims description 11
229910001385 heavy metal Inorganic materials 0.000 claims description 10
239000013522 chelant Substances 0.000 claims description 7
239000007864 aqueous solution Substances 0.000 claims description 4
230000001580 bacterial effect Effects 0.000 claims description 4
230000000087 stabilizing effect Effects 0.000 claims description 4
150000001298 alcohols Chemical class 0.000 claims description 3
150000001413 amino acids Chemical class 0.000 claims description 3
239000000337 buffer salt Substances 0.000 abstract 1
108090000790 Enzymes Proteins 0.000 description 26
102000004190 Enzymes Human genes 0.000 description 26
238000000034 method Methods 0.000 description 23
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 20
238000006243 chemical reaction Methods 0.000 description 20
239000012141 concentrate Substances 0.000 description 18
235000008504 concentrate Nutrition 0.000 description 18
239000007995 HEPES buffer Substances 0.000 description 11
239000003153 chemical reaction reagent Substances 0.000 description 11
229960004418 trolamine Drugs 0.000 description 11
229910021529 ammonia Inorganic materials 0.000 description 9
238000003556 assay Methods 0.000 description 9
239000012153 distilled water Substances 0.000 description 8
238000002156 mixing Methods 0.000 description 8
239000000047 product Substances 0.000 description 8
238000004458 analytical method Methods 0.000 description 7
239000007788 liquid Substances 0.000 description 7
230000008859 change Effects 0.000 description 6
239000002253 acid Substances 0.000 description 5
238000004806 packaging method and process Methods 0.000 description 5
150000003839 salts Chemical class 0.000 description 5
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
229910002092 carbon dioxide Inorganic materials 0.000 description 4
238000005259 measurement Methods 0.000 description 4
229910021645 metal ion Inorganic materials 0.000 description 4
238000003908 quality control method Methods 0.000 description 4
239000012142 reagent concentrate Substances 0.000 description 4
ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 3
239000007983 Tris buffer Substances 0.000 description 3
238000008083 Urea Assay Methods 0.000 description 3
239000001099 ammonium carbonate Substances 0.000 description 3
235000012501 ammonium carbonate Nutrition 0.000 description 3
238000013459 approach Methods 0.000 description 3
230000008901 benefit Effects 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
230000002596 correlated effect Effects 0.000 description 3
238000004108 freeze drying Methods 0.000 description 3
239000004615 ingredient Substances 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
230000006641 stabilisation Effects 0.000 description 3
238000011105 stabilization Methods 0.000 description 3
239000002699 waste material Substances 0.000 description 3
241000894006 Bacteria Species 0.000 description 2
QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 2
HSRJKNPTNIJEKV-UHFFFAOYSA-N Guaifenesin Chemical compound COC1=CC=CC=C1OCC(O)CO HSRJKNPTNIJEKV-UHFFFAOYSA-N 0.000 description 2
BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 2
101000833181 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Glycerol dehydrogenase 1 Proteins 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
238000007792 addition Methods 0.000 description 2
239000001569 carbon dioxide Substances 0.000 description 2
238000009792 diffusion process Methods 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
-1 disodium salt Chemical class 0.000 description 2
239000012467 final product Substances 0.000 description 2
230000001965 increasing effect Effects 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
230000007774 longterm Effects 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
150000002739 metals Chemical class 0.000 description 2
239000007787 solid Substances 0.000 description 2
239000012086 standard solution Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
238000012360 testing method Methods 0.000 description 2
IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 description 1
101710088194 Dehydrogenase Proteins 0.000 description 1
101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
229910019093 NaOCl Inorganic materials 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
108091006629 SLC13A2 Proteins 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
230000003139 buffering effect Effects 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000005515 coenzyme Substances 0.000 description 1
230000000536 complexating effect Effects 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
239000000470 constituent Substances 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
230000000875 corresponding effect Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000002242 deionisation method Methods 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
230000002939 deleterious effect Effects 0.000 description 1
238000002405 diagnostic procedure Methods 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000004821 distillation Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
238000009472 formulation Methods 0.000 description 1
ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
239000003365 glass fiber Substances 0.000 description 1
229930195712 glutamate Natural products 0.000 description 1
JFCQEDHGNNZCLN-UHFFFAOYSA-N glutaric acid Chemical compound OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 1
239000012456 homogeneous solution Substances 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
239000006193 liquid solution Substances 0.000 description 1
239000000463 material Substances 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
238000011328 necessary treatment Methods 0.000 description 1
229910000069 nitrogen hydride Inorganic materials 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000002574 poison Substances 0.000 description 1
231100000614 poison Toxicity 0.000 description 1
239000000843 powder Substances 0.000 description 1
230000008569 process Effects 0.000 description 1
230000036647 reaction Effects 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000011282 treatment Methods 0.000 description 1
239000012224 working solution Substances 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA000366092A 1980-12-03 1980-12-03 Solution enzymatique pour le dosage de l'uree Expired CA1167747A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000366092A CA1167747A (fr)	1980-12-03	1980-12-03	Solution enzymatique pour le dosage de l'uree

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000366092A CA1167747A (fr)	1980-12-03	1980-12-03	Solution enzymatique pour le dosage de l'uree

Publications (1)

Publication Number	Publication Date
CA1167747A true CA1167747A (fr)	1984-05-22

Family

ID=4118631

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000366092A Expired CA1167747A (fr)	1980-12-03	1980-12-03	Solution enzymatique pour le dosage de l'uree

Country Status (1)

Country	Link
CA (1)	CA1167747A (fr)

1980
- 1980-12-03 CA CA000366092A patent/CA1167747A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2001-05-22	MKEX	Expiry

Publication	Publication Date	Title
US4282316A (en)	1981-08-04	Stabilized enzymic solutions for determining urea
US4378430A (en)	1983-03-29	Method of forming stabilized urease solutions
EP0034213B1 (fr)	1984-11-07	Solution aqueuse de co-enzyme stabilisée pour l'utilisation dans un essai clinique, procédé de stabilisation d'une co-enzyme labile dans une solution aqueuse d'essai clinique et une trousse à réactifs pour utilisation dans ledit essai
RU2054674C1 (ru)	1996-02-20	Способ определения концентрации ионов калия в биологическом материале
Kusche et al.	1973	Comparison of the14C-putrescine assay with the NADH test for the determination of diamine oxidase: description of a standard procedure with a high precision and an improved accuracy
US4465770A (en)	1984-08-14	Stabilized enzymic solutions for determining urea
JP2539225B2 (ja)	1996-10-02	グルコ−ス定量用安定化液体酵素組成物、それを用いる試薬キット及び定量方法
CN113092746B (zh)	2022-11-18	生化校准物质
JP2854995B2 (ja)	1999-02-10	尿酸測定用試薬組成物
US5278044A (en)	1994-01-11	Stable aqueous NADH reagent and kit
EP0570588A1 (fr)	1993-11-24	Procede et reactif pour determiner le fer serique ou la capacite de fixation de fer sans saturation
Perakis et al.	1984	Kinetic approach for the enzymic determination of creatinine.
EP0636885B1 (fr)	2002-04-17	Méthode pour déterminer le taux de la fructosamine
Bruce et al.	1978	Two-point determination of plasma ammonia with the centrifugal analyzer.
JP3934498B2 (ja)	2007-06-20	プロテアーゼ含有試薬を用いた定量方法および定量試薬
JP4130724B2 (ja)	2008-08-06	キレート物質を含む測定試薬
US3539453A (en)	1970-11-10	Reagent and method for assaying lactate dehydrogenase
US3546074A (en)	1970-12-08	Reagent for assaying glutamate oxaloacetate transaminase
Girotti et al.	1988	Bioluminescence flow system for determination of branched-chain L-amino acids in serum and urine
CA1204047A (fr)	1986-05-06	Dosage de l'acide urique et reactif utilise a cet effet
US5888828A (en)	1999-03-30	Kit for measuring urea nitrogen
JP2001061498A (ja)	2001-03-13	還元型補酵素溶液
EP0721986A2 (fr)	1996-07-17	Réactif pour créatine-kinase
JPH0155880B2 (fr)	1989-11-28
JP4651237B2 (ja)	2011-03-16	還元型ニコチンアミドアデニンジヌクレオチド類の安定化方法及び試薬