CA1177420A - Vehicules de clonage - Google Patents
Vehicules de clonageInfo
- Publication number
- CA1177420A CA1177420A CA000423778A CA423778A CA1177420A CA 1177420 A CA1177420 A CA 1177420A CA 000423778 A CA000423778 A CA 000423778A CA 423778 A CA423778 A CA 423778A CA 1177420 A CA1177420 A CA 1177420A
- Authority
- CA
- Canada
- Prior art keywords
- plasmid
- dna
- bacterial
- pro1600
- rings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 275
- 238000010367 cloning Methods 0.000 title claims abstract description 36
- 108020004414 DNA Proteins 0.000 claims abstract description 138
- 230000001580 bacterial effect Effects 0.000 claims abstract description 78
- 239000012634 fragment Substances 0.000 claims abstract description 58
- 238000000034 method Methods 0.000 claims abstract description 53
- 241000894006 Bacteria Species 0.000 claims abstract description 48
- 230000008569 process Effects 0.000 claims abstract description 37
- 102000053602 DNA Human genes 0.000 claims abstract description 31
- 101100300704 Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) queF gene Proteins 0.000 claims abstract description 23
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 17
- 101100112724 Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) ccsB gene Proteins 0.000 claims abstract description 14
- 210000000349 chromosome Anatomy 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 31
- 108091008146 restriction endonucleases Proteins 0.000 claims description 26
- 230000010076 replication Effects 0.000 claims description 9
- 238000007792 addition Methods 0.000 claims description 7
- 108010042407 Endonucleases Proteins 0.000 claims description 5
- 102000004533 Endonucleases Human genes 0.000 claims description 5
- 230000032823 cell division Effects 0.000 claims description 5
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 239000003981 vehicle Substances 0.000 claims 2
- 238000010353 genetic engineering Methods 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 2
- 241000364057 Peoria Species 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 description 31
- 229960003669 carbenicillin Drugs 0.000 description 30
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 30
- 238000002474 experimental method Methods 0.000 description 20
- 230000003115 biocidal effect Effects 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 18
- 239000004098 Tetracycline Substances 0.000 description 17
- 229960002180 tetracycline Drugs 0.000 description 17
- 229930101283 tetracycline Natural products 0.000 description 17
- 235000019364 tetracycline Nutrition 0.000 description 17
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 16
- 150000003522 tetracyclines Chemical class 0.000 description 16
- 230000012010 growth Effects 0.000 description 15
- 101100407030 Arabidopsis thaliana PAO2 gene Proteins 0.000 description 14
- 229940024606 amino acid Drugs 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 14
- 150000001413 amino acids Chemical class 0.000 description 14
- 230000009466 transformation Effects 0.000 description 14
- 230000029087 digestion Effects 0.000 description 12
- 238000012423 maintenance Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 108020004511 Recombinant DNA Proteins 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 10
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 9
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 9
- 229960000310 isoleucine Drugs 0.000 description 9
- 229960004452 methionine Drugs 0.000 description 9
- 229960004295 valine Drugs 0.000 description 9
- 230000015572 biosynthetic process Effects 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 230000010310 bacterial transformation Effects 0.000 description 7
- 238000003776 cleavage reaction Methods 0.000 description 7
- 238000001727 in vivo Methods 0.000 description 7
- 230000007017 scission Effects 0.000 description 7
- 229930182844 L-isoleucine Natural products 0.000 description 6
- 239000013611 chromosomal DNA Substances 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 230000002503 metabolic effect Effects 0.000 description 6
- 230000006798 recombination Effects 0.000 description 6
- 238000005215 recombination Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 5
- 229930195722 L-methionine Natural products 0.000 description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 description 5
- 230000002776 aggregation Effects 0.000 description 5
- 238000004220 aggregation Methods 0.000 description 5
- 230000021615 conjugation Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 230000017105 transposition Effects 0.000 description 5
- 108010072454 CTGCAG-specific type II deoxyribonucleases Proteins 0.000 description 4
- 230000004543 DNA replication Effects 0.000 description 4
- 241000589516 Pseudomonas Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 239000013599 cloning vector Substances 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 238000013507 mapping Methods 0.000 description 4
- 229930182817 methionine Natural products 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000589776 Pseudomonas putida Species 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 210000003578 bacterial chromosome Anatomy 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
- 235000014705 isoleucine Nutrition 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 241000557626 Corvus corax Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000000721 bacterilogical effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000030414 genetic transfer Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 150000003212 purines Chemical class 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000005549 size reduction Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 101000596275 Bacillus subtilis Type II restriction enzyme BglI Proteins 0.000 description 1
- 108700003860 Bacterial Genes Proteins 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 241001643623 Enteles Species 0.000 description 1
- 241001517310 Eria Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000428533 Rhis Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 244000292604 Salvia columbariae Species 0.000 description 1
- 235000012377 Salvia columbariae var. columbariae Nutrition 0.000 description 1
- 235000001498 Salvia hispanica Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000006790 cellular biosynthetic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 235000014167 chia Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical compound C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- WKQCYNCZDDJXEK-UHFFFAOYSA-N simalikalactone C Natural products C1C(C23C)OC(=O)CC3C(C)C(=O)C(O)C2C2(C)C1C(C)C=C(OC)C2=O WKQCYNCZDDJXEK-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- BGRJTUBHPOOWDU-UHFFFAOYSA-N sulpiride Chemical compound CCN1CCCC1CNC(=O)C1=CC(S(N)(=O)=O)=CC=C1OC BGRJTUBHPOOWDU-UHFFFAOYSA-N 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000423778A CA1177420A (fr) | 1980-05-08 | 1983-03-16 | Vehicules de clonage |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US147,563 | 1980-05-08 | ||
| US06/147,563 US4374200A (en) | 1980-05-08 | 1980-05-08 | Broad host range small plasmid rings as cloning vehicles |
| CA000355182A CA1150169A (fr) | 1980-05-08 | 1980-06-30 | Anneaux de plasmides comme vehicule de clonage |
| CA000423778A CA1177420A (fr) | 1980-05-08 | 1983-03-16 | Vehicules de clonage |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000355182A Division CA1150169A (fr) | 1980-05-08 | 1980-06-30 | Anneaux de plasmides comme vehicule de clonage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1177420A true CA1177420A (fr) | 1984-11-06 |
Family
ID=27166729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000423778A Expired CA1177420A (fr) | 1980-05-08 | 1983-03-16 | Vehicules de clonage |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1177420A (fr) |
-
1983
- 1983-03-16 CA CA000423778A patent/CA1177420A/fr not_active Expired
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Olsen et al. | Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome | |
| Mann et al. | Cloning of restriction and modification genes in E. coli: the HhaII system from Haemophilus haemolyticus | |
| Timmis et al. | Cloning and characterization of Eco RI and Hin dIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6 | |
| Bradley | Characteristics and function of thick and thin conjugative pili determined by transfer-derepressed plasmids of incompatibility groups I1, I2, I5, B, K and Z | |
| Lecadet et al. | Generalized transduction in Bacillus thuringiensis var. berliner 1715 using bacteriophage CP-54Ber | |
| US4375514A (en) | Preparation and use of recombinant plasmids containing genes for alkaline phosphatases | |
| JPH0691827B2 (ja) | 新規ベクタ−プラスミド | |
| GB2048894A (en) | Plasmid | |
| CA1150169A (fr) | Anneaux de plasmides comme vehicule de clonage | |
| EP0060663A2 (fr) | Procédé pour la transformation de certains micro-organismes, vecteurs et leur production, et procédé pour la production de certaines substances utilisant les vecteurs precités | |
| US4360597A (en) | Streptomyces plasmid and culture | |
| FR2559781A1 (fr) | Nouveau vecteur plasmidique hybride de e. coli conferant le pouvoir de faire fermenter le saccharose et son procede de preparation | |
| Morgan et al. | Genetic organization and regulation of proteins associated with production of syringotoxin by Pseudomonas syringae pv. syringae | |
| US4376164A (en) | Process for preparing broad host range small plasmid rings as cloning vehicles | |
| EP0091723A2 (fr) | Système de vecteur | |
| Foster et al. | Genetic Analysis of Deletions of R1001 that are Both Transfer-deficient and Tetracycline-sensitive | |
| US4418194A (en) | DNA Fragments for forming plasmids | |
| US4717666A (en) | Cloned streptomycete lividans excretable β-galactosidase gene | |
| Cowman et al. | Molecular cloning of the gene (ush) from Escherichia coli specifying periplasmic UDP-sugar hydrolase (5'-nucleotidase) | |
| JPS62122585A (ja) | 宿主・ベクタ−系 | |
| US4686184A (en) | Gene transfer vector | |
| US5049501A (en) | Production method for PvuI restriction endonuclease | |
| EP0179025A1 (fr) | Méthode de production de protéase neutre | |
| KR870001127B1 (ko) | 미생물의 유전적 변형방법 | |
| JP2949352B2 (ja) | プラスミド▲下p▼NI20及びそれをベクターとして用いた遺伝子組換え法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |