CA1177424A - Compose pour culture de cellules - Google Patents

Compose pour culture de cellules

Info

Publication number: CA1177424A
Authority: CA; Canada
Prior art keywords: mammalian; thymidine; biological fluid; natural; cells
Prior art date: 1980-09-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000386521A

Other languages

English (en)

Inventor

Una S. Ryan

James W. Ryan

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of Miami

Original Assignee

University of Miami

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-09-26

Filing date

1981-09-23

Publication date

1984-11-06

1981-09-23 Application filed by University of Miami filed Critical University of Miami

1984-11-06 Application granted granted Critical

1984-11-06 Publication of CA1177424A publication Critical patent/CA1177424A/fr

Status Expired legal-status Critical Current

Links

239000000203 mixture Substances 0.000 title claims description 38
238000004113 cell culture Methods 0.000 title description 15
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims abstract description 112
239000002609 medium Substances 0.000 claims abstract description 97
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims abstract description 56
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims abstract description 56
229940104230 thymidine Drugs 0.000 claims abstract description 56
108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 44
210000002889 endothelial cell Anatomy 0.000 claims abstract description 38
210000000329 smooth muscle myocyte Anatomy 0.000 claims abstract description 34
239000012091 fetal bovine serum Substances 0.000 claims abstract description 32
239000012530 fluid Substances 0.000 claims abstract description 30
239000013060 biological fluid Substances 0.000 claims abstract description 27
235000015097 nutrients Nutrition 0.000 claims abstract description 19
230000012010 growth Effects 0.000 claims abstract description 18
210000004962 mammalian cell Anatomy 0.000 claims abstract description 8
239000006143 cell culture medium Substances 0.000 claims abstract 2
210000004027 cell Anatomy 0.000 claims description 47
210000002966 serum Anatomy 0.000 claims description 40
238000000034 method Methods 0.000 claims description 35
244000309466 calf Species 0.000 claims description 14
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210000001519 tissue Anatomy 0.000 claims description 13
238000012258 culturing Methods 0.000 claims description 12
239000012894 fetal calf serum Substances 0.000 claims description 12
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CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 9
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OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
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FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
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VVIAGPKUTFNRDU-UHFFFAOYSA-N 6S-folinic acid Natural products C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-UHFFFAOYSA-N 0.000 description 2
MPJKWIXIYCLVCU-UHFFFAOYSA-N Folinic acid Natural products NC1=NC2=C(N(C=O)C(CNc3ccc(cc3)C(=O)NC(CCC(=O)O)CC(=O)O)CN2)C(=O)N1 MPJKWIXIYCLVCU-UHFFFAOYSA-N 0.000 description 2
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OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
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APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 2
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235000019152 folic acid Nutrition 0.000 description 2
239000011724 folic acid Substances 0.000 description 2
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235000008191 folinic acid Nutrition 0.000 description 2
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238000002955 isolation Methods 0.000 description 2
229960001691 leucovorin Drugs 0.000 description 2
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KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
239000004033 plastic Substances 0.000 description 2
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239000000843 powder Substances 0.000 description 2
229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
239000000126 substance Substances 0.000 description 2
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210000004509 vascular smooth muscle cell Anatomy 0.000 description 2
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UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
102100031476 Cytochrome P450 1A1 Human genes 0.000 description 1
239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
102000001690 Factor VIII Human genes 0.000 description 1
108010054218 Factor VIII Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
101000941690 Homo sapiens Cytochrome P450 1A1 Proteins 0.000 description 1
102000014150 Interferons Human genes 0.000 description 1
108010050904 Interferons Proteins 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 1
108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
108010039918 Polylysine Proteins 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
210000002403 aortic endothelial cell Anatomy 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
239000011575 calcium Substances 0.000 description 1
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KVUAALJSMIVURS-ZEDZUCNESA-L calcium folinate Chemical compound [Ca+2].C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC([O-])=O)C([O-])=O)C=C1 KVUAALJSMIVURS-ZEDZUCNESA-L 0.000 description 1
ZFXVRMSLJDYJCH-UHFFFAOYSA-N calcium magnesium Chemical compound [Mg].[Ca] ZFXVRMSLJDYJCH-UHFFFAOYSA-N 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000019522 cellular metabolic process Effects 0.000 description 1
239000013043 chemical agent Substances 0.000 description 1
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210000003837 chick embryo Anatomy 0.000 description 1
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239000008367 deionised water Substances 0.000 description 1
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230000001605 fetal effect Effects 0.000 description 1
238000001914 filtration Methods 0.000 description 1
VVIAGPKUTFNRDU-ABLWVSNPSA-N folinic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-ABLWVSNPSA-N 0.000 description 1
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239000011777 magnesium Substances 0.000 description 1
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229940049954 penicillin Drugs 0.000 description 1
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150000003839 salts Chemical class 0.000 description 1
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230000001954 sterilising effect Effects 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
238000012090 tissue culture technique Methods 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
210000003934 vacuole Anatomy 0.000 description 1
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235000013343 vitamin Nutrition 0.000 description 1
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230000003442 weekly effect Effects 0.000 description 1

Landscapes

Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA000386521A 1980-09-26 1981-09-23 Compose pour culture de cellules Expired CA1177424A (fr)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date
US19114580A	1980-09-26	1980-09-26
US191,145		1980-09-26
US22638481A	1981-01-19	1981-01-19
US226,384		1981-01-19

Publications (1)

Publication Number	Publication Date
CA1177424A true CA1177424A (fr)	1984-11-06

Family

ID=26886800

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000386521A Expired CA1177424A (fr)	1980-09-26	1981-09-23	Compose pour culture de cellules

Country Status (1)

Country	Link
CA (1)	CA1177424A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2284254A1 (fr)	2009-07-28	2011-02-16	Grifols, S.A.	Milieux pour la culture de cellules de mammiferes comprenant les surnagenants obtenus a partir des etapes de fractionnement Cohn et leurs utilisations.

1981
- 1981-09-23 CA CA000386521A patent/CA1177424A/fr not_active Expired

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2284254A1 (fr)	2009-07-28	2011-02-16	Grifols, S.A.	Milieux pour la culture de cellules de mammiferes comprenant les surnagenants obtenus a partir des etapes de fractionnement Cohn et leurs utilisations.
US8252590B2 (en)	2009-07-28	2012-08-28	Grifols, S.A.	Mammalian cell culture media which comprise supernatant from cohn fractionation stages and use thereof
EP2826854A1 (fr)	2009-07-28	2015-01-21	Grifols, S.A.	Milieu de culture de cellules mammifères comprenant un surnageant à partir d'étapes de fractionnement de Cohn et utilisation associée

Legal Events

Date	Code	Title	Description
2001-11-06	MKEX	Expiry

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