CA1208146A - Methode pour le transfert de genes dans des cellules - Google Patents

Methode pour le transfert de genes dans des cellules

Info

Publication number: CA1208146A
Authority: CA; Canada
Prior art keywords: cells; genes; gene; transferred; mixture
Prior art date: 1983-10-27
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000439852A

Other languages

English (en)

Inventor

Tai-Kin Wong

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

WONG TAI KIN

Original Assignee

WONG TAI KIN

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-10-27

Filing date

1983-10-27

Publication date

1986-07-22

1983-10-27 Application filed by WONG TAI KIN filed Critical WONG TAI KIN

1983-10-27 Priority to CA000439852A priority Critical patent/CA1208146A/fr

1986-07-22 Application granted granted Critical

1986-07-22 Publication of CA1208146A publication Critical patent/CA1208146A/fr

Status Expired legal-status Critical Current

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 57
238000000034 method Methods 0.000 title claims abstract description 22
239000000203 mixture Substances 0.000 claims abstract description 15
210000004027 cell Anatomy 0.000 claims description 65
230000005684 electric field Effects 0.000 claims description 10
239000000725 suspension Substances 0.000 claims description 9
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
210000004962 mammalian cell Anatomy 0.000 claims description 3
241000187747 Streptomyces Species 0.000 claims description 2
239000013602 bacteriophage vector Substances 0.000 claims description 2
239000013600 plasmid vector Substances 0.000 claims description 2
239000013598 vector Substances 0.000 claims description 2
239000013603 viral vector Substances 0.000 claims description 2
238000011282 treatment Methods 0.000 abstract description 3
239000013612 plasmid Substances 0.000 description 19
239000000243 solution Substances 0.000 description 11
238000012546 transfer Methods 0.000 description 8
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
108020004440 Thymidine kinase Proteins 0.000 description 6
241000588724 Escherichia coli Species 0.000 description 5
239000004098 Tetracycline Substances 0.000 description 5
239000000523 sample Substances 0.000 description 5
239000000126 substance Substances 0.000 description 5
229960002180 tetracycline Drugs 0.000 description 5
229930101283 tetracycline Natural products 0.000 description 5
235000019364 tetracycline Nutrition 0.000 description 5
229960000723 ampicillin Drugs 0.000 description 4
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 4
229910052753 mercury Inorganic materials 0.000 description 4
150000003522 tetracyclines Chemical class 0.000 description 4
229920001817 Agar Polymers 0.000 description 3
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 3
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
102000006601 Thymidine Kinase Human genes 0.000 description 3
239000008272 agar Substances 0.000 description 3
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 3
238000012512 characterization method Methods 0.000 description 3
238000010367 cloning Methods 0.000 description 3
239000008101 lactose Substances 0.000 description 3
238000010297 mechanical methods and process Methods 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
238000012552 review Methods 0.000 description 3
229940104230 thymidine Drugs 0.000 description 3
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
241000196324 Embryophyta Species 0.000 description 2
208000009889 Herpes Simplex Diseases 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
238000004113 cell culture Methods 0.000 description 2
238000012217 deletion Methods 0.000 description 2
230000037430 deletion Effects 0.000 description 2
238000010586 diagram Methods 0.000 description 2
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
239000012894 fetal calf serum Substances 0.000 description 2
239000012634 fragment Substances 0.000 description 2
230000014509 gene expression Effects 0.000 description 2
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
238000011534 incubation Methods 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
239000002609 medium Substances 0.000 description 2
239000002953 phosphate buffered saline Substances 0.000 description 2
TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
244000000626 Daucus carota Species 0.000 description 1
235000002767 Daucus carota Nutrition 0.000 description 1
101100130497 Drosophila melanogaster Mical gene Proteins 0.000 description 1
241001131785 Escherichia coli HB101 Species 0.000 description 1
241001596967 Escherichia coli M15 Species 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
241000162682 Heterogen Species 0.000 description 1
101000852483 Homo sapiens Interleukin-1 receptor-associated kinase 1 Proteins 0.000 description 1
240000005979 Hordeum vulgare Species 0.000 description 1
235000007340 Hordeum vulgare Nutrition 0.000 description 1
108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 1
102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
102000014150 Interferons Human genes 0.000 description 1
108010050904 Interferons Proteins 0.000 description 1
102100036342 Interleukin-1 receptor-associated kinase 1 Human genes 0.000 description 1
241000726306 Irus Species 0.000 description 1
241001625930 Luria Species 0.000 description 1
239000006137 Luria-Bertani broth Substances 0.000 description 1
235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
101100345589 Mus musculus Mical1 gene Proteins 0.000 description 1
244000061176 Nicotiana tabacum Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
206010034133 Pathogen resistance Diseases 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
239000012980 RPMI-1640 medium Substances 0.000 description 1
240000003768 Solanum lycopersicum Species 0.000 description 1
241000700605 Viruses Species 0.000 description 1
108010027570 Xanthine phosphoribosyltransferase Proteins 0.000 description 1
229960003896 aminopterin Drugs 0.000 description 1
230000010310 bacterial transformation Effects 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
244000309466 calf Species 0.000 description 1
229910002092 carbon dioxide Inorganic materials 0.000 description 1
239000001569 carbon dioxide Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
238000010276 construction Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
235000013601 eggs Nutrition 0.000 description 1
210000002257 embryonic structure Anatomy 0.000 description 1
NSYZCCDSJNWWJL-YXOIYICCSA-N erythromycin ethylsuccinate Chemical compound O1[C@H](C)C[C@H](N(C)C)[C@@H](OC(=O)CCC(=O)OCC)[C@@H]1O[C@H]1[C@@](O)(C)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@](C)(O)[C@@H](CC)OC(=O)[C@H](C)[C@@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(OC)C2)[C@@H]1C NSYZCCDSJNWWJL-YXOIYICCSA-N 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000003550 marker Substances 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
210000000287 oocyte Anatomy 0.000 description 1
229940086255 perform Drugs 0.000 description 1
238000007747 plating Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
-1 such as Substances 0.000 description 1
OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
239000012137 tryptone Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA000439852A 1983-10-27 1983-10-27 Methode pour le transfert de genes dans des cellules Expired CA1208146A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000439852A CA1208146A (fr)	1983-10-27	1983-10-27	Methode pour le transfert de genes dans des cellules

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000439852A CA1208146A (fr)	1983-10-27	1983-10-27	Methode pour le transfert de genes dans des cellules

Publications (1)

Publication Number	Publication Date
CA1208146A true CA1208146A (fr)	1986-07-22

Family

ID=4126389

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000439852A Expired CA1208146A (fr)	1983-10-27	1983-10-27	Methode pour le transfert de genes dans des cellules

Country Status (1)

Country	Link
CA (1)	CA1208146A (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0319690A1 (fr) *	1987-11-24	1989-06-14	Nisshin Flour Milling Co., Ltd.	Procédé pour l'introduction d'ADN étranger dans certaines bactéries
EP0290395A3 (en) *	1987-05-05	1990-11-28	Sandoz Ag	Plant tissue transformation
US5231019A (en) *	1984-05-11	1993-07-27	Ciba-Geigy Corporation	Transformation of hereditary material of plants
US5232856A (en) *	1990-06-25	1993-08-03	Firth Kevin L	Electroporation device
US5371003A (en) *	1987-05-05	1994-12-06	Sandoz Ltd.	Electrotransformation process
EP0624100A4 (en) *	1992-11-03	1995-11-29	Synaptic Pharma Corp	Dna encoding a human serotonin receptor (5-ht 4b?) and uses thereof.
US6300087B1 (en)	1992-11-03	2001-10-09	Synaptic Pharmaceutical Corporation	DNA encoding a human serotonin receptor (5-HT4B) and uses thereof

1983
- 1983-10-27 CA CA000439852A patent/CA1208146A/fr not_active Expired

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5231019A (en) *	1984-05-11	1993-07-27	Ciba-Geigy Corporation	Transformation of hereditary material of plants
US6201169B1 (en)	1984-05-11	2001-03-13	Novartis Finance Corporation	Transformation of hereditary material of Brassica plants and cells
US6603065B2 (en)	1984-05-11	2003-08-05	Syngenta Invest Corp	Transformation of hereditary material of plants
EP0290395A3 (en) *	1987-05-05	1990-11-28	Sandoz Ag	Plant tissue transformation
EP0574356A1 (fr) *	1987-05-05	1993-12-15	Sandoz Ag	Transformation de tissues de plantes
US5371003A (en) *	1987-05-05	1994-12-06	Sandoz Ltd.	Electrotransformation process
EP0319690A1 (fr) *	1987-11-24	1989-06-14	Nisshin Flour Milling Co., Ltd.	Procédé pour l'introduction d'ADN étranger dans certaines bactéries
US5232856A (en) *	1990-06-25	1993-08-03	Firth Kevin L	Electroporation device
EP0624100A4 (en) *	1992-11-03	1995-11-29	Synaptic Pharma Corp	Dna encoding a human serotonin receptor (5-ht 4b?) and uses thereof.
US6300087B1 (en)	1992-11-03	2001-10-09	Synaptic Pharmaceutical Corporation	DNA encoding a human serotonin receptor (5-HT4B) and uses thereof
US6376243B1 (en)	1992-11-03	2002-04-23	Synaptic Pharmaceutical Corporation	DNA encoding a human serotonin receptor (5-HT4b) and uses thereof
US6432655B1 (en)	1992-11-03	2002-08-13	Synaptic Pharmaceutical Corporation	Method of obtaining compositions

Legal Events

Date	Code	Title	Description
2003-10-27	MKEX	Expiry

Publication	Publication Date	Title
US4849355A (en)	1989-07-18	Method of transferring genes into cells
Fishel et al.	1981	recA-independent general genetic recombination of plasmids
JP2588917B2 (ja)	1997-03-12	２，４‐ジクロルフエノキシ酢酸（２，４‐ｄ）‐モノオキシゲナーゼ形成用の微生物及びプラスミド並びにこれらのプラスミド及び菌株の製法
Hershfield et al.	1976	Characterization of a mini-ColC1 plasmid
Fleckenstein et al.	1982	Cloning of the complete human cytomegalovirus genome in cosmids
Meyer et al.	1977	Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2
Gryczan et al.	1978	Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis
Ball et al.	1991	Efficient excision of phage lambda from the Escherichia coli chromosome requires the Fis protein
Rella et al.	1985	Transposon insertion mutagenesis of Pseudomonas aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster
US5869296A (en)	1999-02-09	DNA transposon Tn5seq1
Sugino et al.	1980	Bacillus subtilis deoxyribonucleic acid gyrase
Huber et al.	1985	Site-specific DNA inversion is enhanced by a DNA sequence element in cis
Bonekamp et al.	1984	Mechanism of UTP‐modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription.
HU197043B (en)	1989-02-28	Process for the stabilization and selection of host-cells comprising recombinant dna
Sakakibara et al.	1980	A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaA, a new gene adjacent to the dnA, gene
Kahn et al.	1979	Essential regions for replication of a stringent and a relaxed plasmid in Escherichia coli
Diaz et al.	1981	Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli
Howe	1973	Transduction by bacteriophage Mu-1
Kooistra et al.	1988	Cloning and characterization of a Bacillus subtilis transcription unit involved in ATP-dependent DNase synthesis
US5567607A (en)	1996-10-22	Method of producing transgenic animals
Shattuck-Eidens et al.	1983	Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli
Lacks et al.	1984	Cloning in Streptococcus pneumoniae of the gene for DpnII DNA methylase
Bonamy et al.	1982	Cloning and expression of Bacillus subtilis spore genes
Wilson et al.	1978	Characterization and construction of molecular cloning vehicles within Staphylococcus aureus
Mes et al.	1982	Polyoma viral middle T-antigen is required for transformation