CA1216531A - Vecteur de clonage pour cyanobacterium - Google Patents
Vecteur de clonage pour cyanobacteriumInfo
- Publication number
- CA1216531A CA1216531A CA000423616A CA423616A CA1216531A CA 1216531 A CA1216531 A CA 1216531A CA 000423616 A CA000423616 A CA 000423616A CA 423616 A CA423616 A CA 423616A CA 1216531 A CA1216531 A CA 1216531A
- Authority
- CA
- Canada
- Prior art keywords
- plasmid
- dna
- coli
- replication
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000010367 cloning Methods 0.000 title claims abstract description 15
- 239000013598 vector Substances 0.000 title claims abstract description 14
- 241001464430 Cyanobacterium Species 0.000 title claims description 11
- 239000013612 plasmid Substances 0.000 claims abstract description 80
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 26
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 22
- 241000588724 Escherichia coli Species 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000010076 replication Effects 0.000 claims abstract description 11
- 239000012634 fragment Substances 0.000 claims abstract description 10
- 230000003115 biocidal effect Effects 0.000 claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 4
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 3
- 108020004414 DNA Proteins 0.000 claims description 23
- 239000013599 cloning vector Substances 0.000 claims description 13
- 108010042407 Endonucleases Proteins 0.000 claims description 9
- 102000004533 Endonucleases Human genes 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 8
- 101100084627 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pcb-4 gene Proteins 0.000 claims description 6
- 230000006798 recombination Effects 0.000 claims description 5
- 238000005215 recombination Methods 0.000 claims description 5
- 102000012410 DNA Ligases Human genes 0.000 claims description 4
- 108010061982 DNA Ligases Proteins 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 108700026220 vif Genes Proteins 0.000 claims 1
- 238000010353 genetic engineering Methods 0.000 abstract description 4
- 239000013605 shuttle vector Substances 0.000 abstract description 4
- 108700001094 Plant Genes Proteins 0.000 abstract description 2
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000003776 cleavage reaction Methods 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical compound C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 241000192589 Synechococcus elongatus PCC 7942 Species 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 241000985694 Polypodiopsida Species 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 125000004093 cyano group Chemical group *C#N 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000989 food dye Substances 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000423616A CA1216531A (fr) | 1983-03-15 | 1983-03-15 | Vecteur de clonage pour cyanobacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000423616A CA1216531A (fr) | 1983-03-15 | 1983-03-15 | Vecteur de clonage pour cyanobacterium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1216531A true CA1216531A (fr) | 1987-01-13 |
Family
ID=4124784
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000423616A Expired CA1216531A (fr) | 1983-03-15 | 1983-03-15 | Vecteur de clonage pour cyanobacterium |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1216531A (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2637293A1 (fr) * | 1988-07-26 | 1990-04-06 | Commissariat Energie Atomique | Plasmides recombinants, vecteurs comprenant lesdits plasmides, leurs procedes de production, souche de cyanobacterie synechocystis modifiee, apte a etre hote desdits plasmides et/ou desdits vecteurs |
| EP0533942A4 (en) * | 1991-03-13 | 1994-06-01 | Hagiwara Yoshihide | Process for expressing polypeptide |
| US5804408A (en) * | 1991-03-13 | 1998-09-08 | Yoshihide Hagiwara | Expression of human SOD in blue green algae |
-
1983
- 1983-03-15 CA CA000423616A patent/CA1216531A/fr not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2637293A1 (fr) * | 1988-07-26 | 1990-04-06 | Commissariat Energie Atomique | Plasmides recombinants, vecteurs comprenant lesdits plasmides, leurs procedes de production, souche de cyanobacterie synechocystis modifiee, apte a etre hote desdits plasmides et/ou desdits vecteurs |
| EP0533942A4 (en) * | 1991-03-13 | 1994-06-01 | Hagiwara Yoshihide | Process for expressing polypeptide |
| US5804408A (en) * | 1991-03-13 | 1998-09-08 | Yoshihide Hagiwara | Expression of human SOD in blue green algae |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |