CA1228053A - Separation magnetique a l'aide d'ions magnetiques chelates - Google Patents

Separation magnetique a l'aide d'ions magnetiques chelates

Info

Publication number: CA1228053A
Authority: CA; Canada
Prior art keywords: cells; particles; magnetic; chelate; chelated
Prior art date: 1983-10-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000464624A

Other languages

English (en)

Inventor

Marshall D. Graham

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Coulter Electronics Inc

Original Assignee

Coulter Electronics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-10-06

Filing date

1984-10-03

Publication date

1987-10-13

1983-10-06 Priority claimed from US06/539,542 external-priority patent/US4508625A/en

1984-10-03 Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc

1987-10-13 Application granted granted Critical

1987-10-13 Publication of CA1228053A publication Critical patent/CA1228053A/fr

Status Expired legal-status Critical Current

Links

230000005291 magnetic effect Effects 0.000 title claims abstract description 71
150000002500 ions Chemical class 0.000 title claims abstract description 15
238000007885 magnetic separation Methods 0.000 title abstract description 22
239000002245 particle Substances 0.000 claims abstract description 76
238000000034 method Methods 0.000 claims abstract description 72
230000005298 paramagnetic effect Effects 0.000 claims abstract description 57
239000007788 liquid Substances 0.000 claims abstract description 30
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 22
239000002738 chelating agent Substances 0.000 claims abstract description 9
239000000203 mixture Substances 0.000 claims abstract description 5
239000011146 organic particle Substances 0.000 claims abstract 2
210000004027 cell Anatomy 0.000 claims description 87
239000013522 chelant Substances 0.000 claims description 60
230000008569 process Effects 0.000 claims description 38
229910021645 metal ion Inorganic materials 0.000 claims description 30
239000011159 matrix material Substances 0.000 claims description 23
229910052747 lanthanoid Inorganic materials 0.000 claims description 17
150000002602 lanthanoids Chemical class 0.000 claims description 16
229910052691 Erbium Inorganic materials 0.000 claims description 14
UYAHIZSMUZPPFV-UHFFFAOYSA-N erbium Chemical compound [Er] UYAHIZSMUZPPFV-UHFFFAOYSA-N 0.000 claims description 14
239000000463 material Substances 0.000 claims description 14
238000011010 flushing procedure Methods 0.000 claims description 12
229910052692 Dysprosium Inorganic materials 0.000 claims description 8
KBQHZAAAGSGFKK-UHFFFAOYSA-N dysprosium atom Chemical compound [Dy] KBQHZAAAGSGFKK-UHFFFAOYSA-N 0.000 claims description 8
239000000126 substance Substances 0.000 claims description 8
239000002253 acid Substances 0.000 claims description 6
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
230000009920 chelation Effects 0.000 claims description 4
229910052688 Gadolinium Inorganic materials 0.000 claims description 3
229910052689 Holmium Inorganic materials 0.000 claims description 3
QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 claims description 3
229910052771 Terbium Inorganic materials 0.000 claims description 3
229910052775 Thulium Inorganic materials 0.000 claims description 3
DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims description 3
UIWYJDYFSGRHKR-UHFFFAOYSA-N gadolinium atom Chemical compound [Gd] UIWYJDYFSGRHKR-UHFFFAOYSA-N 0.000 claims description 3
KJZYNXUDTRRSPN-UHFFFAOYSA-N holmium atom Chemical compound [Ho] KJZYNXUDTRRSPN-UHFFFAOYSA-N 0.000 claims description 3
GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 claims description 3
URDCARMUOSMFFI-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(2-hydroxyethyl)amino]acetic acid Chemical compound OCCN(CC(O)=O)CCN(CC(O)=O)CC(O)=O URDCARMUOSMFFI-UHFFFAOYSA-N 0.000 claims description 2
RAEOEMDZDMCHJA-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-[2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]ethyl]amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CCN(CC(O)=O)CC(O)=O)CC(O)=O RAEOEMDZDMCHJA-UHFFFAOYSA-N 0.000 claims description 2
JHALWMSZGCVVEM-UHFFFAOYSA-N 2-[4,7-bis(carboxymethyl)-1,4,7-triazonan-1-yl]acetic acid Chemical compound OC(=O)CN1CCN(CC(O)=O)CCN(CC(O)=O)CC1 JHALWMSZGCVVEM-UHFFFAOYSA-N 0.000 claims description 2
-1 HEDHA Chemical compound 0.000 claims description 2
WDLRUFUQRNWCPK-UHFFFAOYSA-N Tetraxetan Chemical compound OC(=O)CN1CCN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC1 WDLRUFUQRNWCPK-UHFFFAOYSA-N 0.000 claims description 2
229910017052 cobalt Inorganic materials 0.000 claims description 2
239000010941 cobalt Substances 0.000 claims description 2
GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
229910052742 iron Inorganic materials 0.000 claims description 2
WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
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108010054147 Hemoglobins Proteins 0.000 abstract description 11
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HDGGAKOVUDZYES-UHFFFAOYSA-K erbium(iii) chloride Chemical compound Cl[Er](Cl)Cl HDGGAKOVUDZYES-UHFFFAOYSA-K 0.000 abstract description 10
150000003839 salts Chemical class 0.000 abstract description 7
MAYVZUQEFSJDHA-UHFFFAOYSA-N 1,5-bis(methylsulfanyl)naphthalene Chemical compound C1=CC=C2C(SC)=CC=CC2=C1SC MAYVZUQEFSJDHA-UHFFFAOYSA-N 0.000 abstract description 3
239000010954 inorganic particle Substances 0.000 abstract description 2
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238000001914 filtration Methods 0.000 description 16
230000027455 binding Effects 0.000 description 13
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
239000000523 sample Substances 0.000 description 12
239000000725 suspension Substances 0.000 description 11
JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 10
230000000717 retained effect Effects 0.000 description 9
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HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
230000005292 diamagnetic effect Effects 0.000 description 6
239000006249 magnetic particle Substances 0.000 description 6
210000003743 erythrocyte Anatomy 0.000 description 5
239000011780 sodium chloride Substances 0.000 description 5
238000004458 analytical method Methods 0.000 description 4
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239000004576 sand Substances 0.000 description 4
241000894007 species Species 0.000 description 4
210000001519 tissue Anatomy 0.000 description 4
UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 3
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239000000872 buffer Substances 0.000 description 3
239000008367 deionised water Substances 0.000 description 3
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238000006392 deoxygenation reaction Methods 0.000 description 3
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230000005408 paramagnetism Effects 0.000 description 3
239000013049 sediment Substances 0.000 description 3
239000011550 stock solution Substances 0.000 description 3
INGWEZCOABYORO-UHFFFAOYSA-N 2-(furan-2-yl)-7-methyl-1h-1,8-naphthyridin-4-one Chemical compound N=1C2=NC(C)=CC=C2C(O)=CC=1C1=CC=CO1 INGWEZCOABYORO-UHFFFAOYSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 2
CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 2
229910021578 Iron(III) chloride Inorganic materials 0.000 description 2
229910021380 Manganese Chloride Inorganic materials 0.000 description 2
GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
241000700605 Viruses Species 0.000 description 2
230000002411 adverse Effects 0.000 description 2
210000004102 animal cell Anatomy 0.000 description 2
239000007900 aqueous suspension Substances 0.000 description 2
150000001669 calcium Chemical class 0.000 description 2
239000000969 carrier Substances 0.000 description 2
230000000052 comparative effect Effects 0.000 description 2
230000007423 decrease Effects 0.000 description 2
108010002255 deoxyhemoglobin Proteins 0.000 description 2
235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 2
GRWZHXKQBITJKP-UHFFFAOYSA-L dithionite(2-) Chemical compound [O-]S(=O)S([O-])=O GRWZHXKQBITJKP-UHFFFAOYSA-L 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
239000012634 fragment Substances 0.000 description 2
235000013980 iron oxide Nutrition 0.000 description 2
RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 2
210000004962 mammalian cell Anatomy 0.000 description 2
239000011565 manganese chloride Substances 0.000 description 2
235000002867 manganese chloride Nutrition 0.000 description 2
229940099607 manganese chloride Drugs 0.000 description 2
239000011368 organic material Substances 0.000 description 2
229960003330 pentetic acid Drugs 0.000 description 2
239000008191 permeabilizing agent Substances 0.000 description 2
239000004033 plastic Substances 0.000 description 2
239000000047 product Substances 0.000 description 2
229910052761 rare earth metal Inorganic materials 0.000 description 2
150000002910 rare earth metals Chemical class 0.000 description 2
230000035939 shock Effects 0.000 description 2
LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
241000003910 Baronia <angiosperm> Species 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
230000005355 Hall effect Effects 0.000 description 1
PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
108010061951 Methemoglobin Proteins 0.000 description 1
239000007990 PIPES buffer Substances 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
206010057249 Phagocytosis Diseases 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
241000022563 Rema Species 0.000 description 1
XDXHAEQXIBQUEZ-UHFFFAOYSA-N Ropinirole hydrochloride Chemical compound Cl.CCCN(CCC)CCC1=CC=CC2=C1CC(=O)N2 XDXHAEQXIBQUEZ-UHFFFAOYSA-N 0.000 description 1
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CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
239000003302 ferromagnetic material Substances 0.000 description 1
238000011049 filling Methods 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
239000008394 flocculating agent Substances 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
230000005484 gravity Effects 0.000 description 1
229910001385 heavy metal Inorganic materials 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
229910021644 lanthanide ion Inorganic materials 0.000 description 1
210000000265 leukocyte Anatomy 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
238000011068 loading method Methods 0.000 description 1
239000000696 magnetic material Substances 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
229910052748 manganese Inorganic materials 0.000 description 1
239000011572 manganese Substances 0.000 description 1
239000002923 metal particle Substances 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
235000010755 mineral Nutrition 0.000 description 1
239000011707 mineral Substances 0.000 description 1
210000003463 organelle Anatomy 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
239000002907 paramagnetic material Substances 0.000 description 1
210000005259 peripheral blood Anatomy 0.000 description 1
239000011886 peripheral blood Substances 0.000 description 1
239000012466 permeate Substances 0.000 description 1
230000008782 phagocytosis Effects 0.000 description 1
HCTVWSOKIJULET-LQDWTQKMSA-M phenoxymethylpenicillin potassium Chemical compound [K+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)COC1=CC=CC=C1 HCTVWSOKIJULET-LQDWTQKMSA-M 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
229960005141 piperazine Drugs 0.000 description 1
210000004623 platelet-rich plasma Anatomy 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
238000012545 processing Methods 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000001846 repelling effect Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
229910000938 samarium–cobalt magnet Inorganic materials 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
235000010288 sodium nitrite Nutrition 0.000 description 1
239000007787 solid Substances 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000008107 starch Substances 0.000 description 1
230000032258 transport Effects 0.000 description 1
UJBPGOAZQSYXNT-UHFFFAOYSA-K trichloroerbium;hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Cl-].[Er+3] UJBPGOAZQSYXNT-UHFFFAOYSA-K 0.000 description 1
PYOOBRULIYNHJR-UHFFFAOYSA-K trichloroholmium Chemical compound Cl[Ho](Cl)Cl PYOOBRULIYNHJR-UHFFFAOYSA-K 0.000 description 1
239000012224 working solution Substances 0.000 description 1

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Investigating Or Analysing Biological Materials (AREA)

CA000464624A 1983-10-06 1984-10-03 Separation magnetique a l'aide d'ions magnetiques chelates Expired CA1228053A (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US539,542		1983-10-06
US06/539,542 US4508625A (en)	1982-10-18	1983-10-06	Magnetic separation using chelated magnetic ions

Publications (1)

Publication Number	Publication Date
CA1228053A true CA1228053A (fr)	1987-10-13

Family

ID=24151669

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000464624A Expired CA1228053A (fr)	1983-10-06	1984-10-03	Separation magnetique a l'aide d'ions magnetiques chelates

Country Status (1)

Country	Link
CA (1)	CA1228053A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN115044550A (zh) *	2022-08-15	2022-09-13	广州鸿溪见杉科技有限公司	一种分离红细胞的方法及装置

1984
- 1984-10-03 CA CA000464624A patent/CA1228053A/fr not_active Expired

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN115044550A (zh) *	2022-08-15	2022-09-13	广州鸿溪见杉科技有限公司	一种分离红细胞的方法及装置

Legal Events

Date	Code	Title	Description
2004-10-13	MKEX	Expiry

Publication	Publication Date	Title
US4508625A (en)	1985-04-02	Magnetic separation using chelated magnetic ions
Owen et al.	1984	Magnetic labeling and cell sorting
JP4713735B2 (ja)	2011-06-29	選択された粒子、特に生物細胞を磁気的に分離するための方法と装置
DE69632785T2 (de)	2005-07-14	Verbessertes verfahren zur herstellung magnetisch empfindlicher teilchen
JP7691475B2 (ja)	2025-06-11	磁気分離
KR20110025975A (ko)	2011-03-14	세포, 병원체 또는 바이러스의 제거를 위한 초상자성 나노입자
US7357859B2 (en)	2008-04-15	Methods and systems for membrane testing
DE102007043281A1 (de)	2009-05-28	Vorrichtung, Materialien und Verfahren zur Hochgradientenmagnetseparation biologischen Materials
CA1228053A (fr)	1987-10-13	Separation magnetique a l'aide d'ions magnetiques chelates
US4366065A (en)	1982-12-28	Separating particles from a liquid
Hartig et al.	1992	Preparative continuous separation of biological particles by means of free‐flow magnetophoresis in a free‐flow electrophoresis chamber
Owen et al.	1983	Ferritin as a label for high-gradient magnetic separation
WO2013113990A1 (fr)	2013-08-08	Séparation de nanomatériaux luminescents
Nakamura et al.	1971	Magneto‐Medicine: Biological Aspects of Ferromagnetic Fine Particles
DE19939208C2 (de)	2001-05-17	Verfahren zum Darstellen von biologisch aktivierten induktivitätsändernden Partikeln zu deren Nachweis und Zählen sowie Vorrichtung dafür
Schröder et al.	1986	Magnetic carbohydrate nanoparticles for affinity cell separation
OWEN	1983	Magnetic cell sorting
Graham	1983	Lanthanides as soluble paramagnetic agents for magnetic filtration of cells
Graham	1984	Comparison of volume and surface mechanisms for magnetic filtration of blood cells
Evans et al.	1986	Demonstration of the principle of paramagnetic chromatography for resolving mixtures of particles
OWEN et al.	1987	Magnetite–Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration
GB1492971A (en)	1977-11-23	Magnetic separation
Evans et al.	1989	Approaches to paramagnetic separations in biology and medicine
HK40051566A (zh)	2022-01-07	磁性分离
HK40051566B (zh)	2025-10-10	磁性分离

CA1228053A - Separation magnetique a l'aide d'ions magnetiques chelates - Google Patents

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Links

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Applications Claiming Priority (2)

Publications (1)

Family

ID=24151669

Family Applications (1)

Country Status (1)

Cited By (1)

Cited By (1)

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