CA1233096A - Methode de fixation des erythrocytes au sant entier - Google Patents
Methode de fixation des erythrocytes au sant entierInfo
- Publication number
- CA1233096A CA1233096A CA000475939A CA475939A CA1233096A CA 1233096 A CA1233096 A CA 1233096A CA 000475939 A CA000475939 A CA 000475939A CA 475939 A CA475939 A CA 475939A CA 1233096 A CA1233096 A CA 1233096A
- Authority
- CA
- Canada
- Prior art keywords
- reagent
- concentration
- sphering
- whole blood
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000003743 erythrocyte Anatomy 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 41
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 39
- 210000004369 blood Anatomy 0.000 claims description 36
- 239000008280 blood Substances 0.000 claims description 34
- 239000003795 chemical substances by application Substances 0.000 claims description 34
- 239000000523 sample Substances 0.000 claims description 22
- 210000004027 cell Anatomy 0.000 claims description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 13
- 239000008363 phosphate buffer Substances 0.000 claims description 10
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 102000001554 Hemoglobins Human genes 0.000 claims description 7
- 108010054147 Hemoglobins Proteins 0.000 claims description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 230000001939 inductive effect Effects 0.000 claims description 6
- -1 alkali metal salt Chemical class 0.000 claims description 5
- 238000005259 measurement Methods 0.000 claims description 5
- 239000012470 diluted sample Substances 0.000 claims description 4
- 229910052783 alkali metal Inorganic materials 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000000149 argon plasma sintering Methods 0.000 claims description 2
- 150000008051 alkyl sulfates Chemical class 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 2
- 230000008859 change Effects 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000009966 trimming Methods 0.000 description 2
- IAKOZHOLGAGEJT-UHFFFAOYSA-N 1,1,1-trichloro-2,2-bis(p-methoxyphenyl)-Ethane Chemical compound C1=CC(OC)=CC=C1C(C(Cl)(Cl)Cl)C1=CC=C(OC)C=C1 IAKOZHOLGAGEJT-UHFFFAOYSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 101700004678 SLIT3 Proteins 0.000 description 1
- 102100027339 Slit homolog 3 protein Human genes 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229920000180 alkyd Polymers 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003455 independent Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000475939A CA1233096A (fr) | 1985-03-07 | 1985-03-07 | Methode de fixation des erythrocytes au sant entier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000475939A CA1233096A (fr) | 1985-03-07 | 1985-03-07 | Methode de fixation des erythrocytes au sant entier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1233096A true CA1233096A (fr) | 1988-02-23 |
Family
ID=4129971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000475939A Expired CA1233096A (fr) | 1985-03-07 | 1985-03-07 | Methode de fixation des erythrocytes au sant entier |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1233096A (fr) |
-
1985
- 1985-03-07 CA CA000475939A patent/CA1233096A/fr not_active Expired
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |