CA1247995A - Methode volumetrique pour l'etablissement de la formule leucocytaire - Google Patents

Methode volumetrique pour l'etablissement de la formule leucocytaire

Info

Publication number: CA1247995A
Authority: CA; Canada
Prior art keywords: lysing reagent; population; reagent; lysing; populations
Prior art date: 1984-10-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000465664A

Other languages

English (en)

Inventor

Stephen L. Ledis

Ted Sena

James H. Ii Carter

Harold R. Crews

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Beckman Coulter Inc

Original Assignee

Coulter Electronics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-10-17

Filing date

1984-10-17

Publication date

1989-01-03

1984-10-17 Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc

1984-10-17 Priority to CA000465664A priority Critical patent/CA1247995A/fr

1989-01-03 Application granted granted Critical

1989-01-03 Publication of CA1247995A publication Critical patent/CA1247995A/fr

Status Expired legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 52
210000000265 leukocyte Anatomy 0.000 title claims abstract description 47
210000004369 blood Anatomy 0.000 claims abstract description 66
230000002934 lysing effect Effects 0.000 claims abstract description 64
239000008280 blood Substances 0.000 claims abstract description 63
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 50
210000001616 monocyte Anatomy 0.000 claims abstract description 49
210000003714 granulocyte Anatomy 0.000 claims abstract description 36
210000004698 lymphocyte Anatomy 0.000 claims abstract description 32
239000003085 diluting agent Substances 0.000 claims abstract description 28
239000000203 mixture Substances 0.000 claims abstract description 21
150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 12
210000000601 blood cell Anatomy 0.000 claims abstract description 10
239000004615 ingredient Substances 0.000 claims abstract description 10
230000004048 modification Effects 0.000 claims abstract description 9
238000012986 modification Methods 0.000 claims abstract description 9
239000007864 aqueous solution Substances 0.000 claims abstract description 7
230000003139 buffering effect Effects 0.000 claims abstract description 5
230000000087 stabilizing effect Effects 0.000 claims abstract description 3
230000002101 lytic effect Effects 0.000 claims description 27
210000004027 cell Anatomy 0.000 claims description 26
238000009826 distribution Methods 0.000 claims description 13
230000035939 shock Effects 0.000 claims description 10
102000001554 Hemoglobins Human genes 0.000 claims description 8
108010054147 Hemoglobins Proteins 0.000 claims description 8
230000004069 differentiation Effects 0.000 claims description 8
DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 claims description 8
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
CXRFDZFCGOPDTD-UHFFFAOYSA-M Cetrimide Chemical compound [Br-].CCCCCCCCCCCCCC[N+](C)(C)C CXRFDZFCGOPDTD-UHFFFAOYSA-M 0.000 claims description 5
239000002245 particle Substances 0.000 claims description 5
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 4
230000002070 germicidal effect Effects 0.000 claims description 4
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims description 3
210000000170 cell membrane Anatomy 0.000 claims description 3
VUFOSBDICLTFMS-UHFFFAOYSA-M ethyl-hexadecyl-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)CC VUFOSBDICLTFMS-UHFFFAOYSA-M 0.000 claims description 3
230000000936 membranestabilizing effect Effects 0.000 claims description 3
150000003839 salts Chemical class 0.000 claims description 3
HCBIBCJNVBAKAB-UHFFFAOYSA-N Procaine hydrochloride Chemical compound Cl.CCN(CC)CCOC(=O)C1=CC=C(N)C=C1 HCBIBCJNVBAKAB-UHFFFAOYSA-N 0.000 claims description 2
QUBQYFYWUJJAAK-UHFFFAOYSA-N oxymethurea Chemical compound OCNC(=O)NCO QUBQYFYWUJJAAK-UHFFFAOYSA-N 0.000 claims description 2
229950005308 oxymethurea Drugs 0.000 claims description 2
229960001309 procaine hydrochloride Drugs 0.000 claims description 2
NBZBKCUXIYYUSX-UHFFFAOYSA-N iminodiacetic acid Chemical compound OC(=O)CNCC(O)=O NBZBKCUXIYYUSX-UHFFFAOYSA-N 0.000 claims 1
210000003743 erythrocyte Anatomy 0.000 abstract description 20
238000004458 analytical method Methods 0.000 abstract description 3
230000003385 bacteriostatic effect Effects 0.000 abstract 1
239000003795 chemical substances by application Substances 0.000 description 34
239000000523 sample Substances 0.000 description 16
230000002159 abnormal effect Effects 0.000 description 13
239000000243 solution Substances 0.000 description 10
238000009472 formulation Methods 0.000 description 8
210000000440 neutrophil Anatomy 0.000 description 8
210000003979 eosinophil Anatomy 0.000 description 7
NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 6
-1 alkali metal cyanide Chemical class 0.000 description 5
210000003651 basophil Anatomy 0.000 description 5
238000010790 dilution Methods 0.000 description 5
239000012895 dilution Substances 0.000 description 5
238000000926 separation method Methods 0.000 description 5
210000001772 blood platelet Anatomy 0.000 description 4
VICYBMUVWHJEFT-UHFFFAOYSA-N dodecyltrimethylammonium ion Chemical compound CCCCCCCCCCCC[N+](C)(C)C VICYBMUVWHJEFT-UHFFFAOYSA-N 0.000 description 4
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
239000004480 active ingredient Substances 0.000 description 3
229910052783 alkali metal Inorganic materials 0.000 description 3
SWLVFNYSXGMGBS-UHFFFAOYSA-N ammonium bromide Chemical compound [NH4+].[Br-] SWLVFNYSXGMGBS-UHFFFAOYSA-N 0.000 description 3
125000004432 carbon atom Chemical group C* 0.000 description 3
230000009089 cytolysis Effects 0.000 description 3
238000007865 diluting Methods 0.000 description 3
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
239000013065 commercial product Substances 0.000 description 2
230000006378 damage Effects 0.000 description 2
230000000694 effects Effects 0.000 description 2
230000003993 interaction Effects 0.000 description 2
238000005259 measurement Methods 0.000 description 2
210000005087 mononuclear cell Anatomy 0.000 description 2
210000000066 myeloid cell Anatomy 0.000 description 2
229910052700 potassium Inorganic materials 0.000 description 2
239000011591 potassium Substances 0.000 description 2
238000004513 sizing Methods 0.000 description 2
239000000725 suspension Substances 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
238000000862 absorption spectrum Methods 0.000 description 1
WDJHALXBUFZDSR-UHFFFAOYSA-N acetoacetic acid Chemical compound CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 1
230000009471 action Effects 0.000 description 1
239000000654 additive Substances 0.000 description 1
125000000217 alkyl group Chemical group 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
239000002585 base Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000004820 blood count Methods 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
238000007374 clinical diagnostic method Methods 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
238000013480 data collection Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
239000012470 diluted sample Substances 0.000 description 1
238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
239000012634 fragment Substances 0.000 description 1
239000011521 glass Substances 0.000 description 1
230000036541 health Effects 0.000 description 1
230000010354 integration Effects 0.000 description 1
208000032839 leukemia Diseases 0.000 description 1
201000002364 leukopenia Diseases 0.000 description 1
231100001022 leukopenia Toxicity 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
210000001237 metamyelocyte Anatomy 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
210000003914 myeloid leukocyte Anatomy 0.000 description 1
230000001575 pathological effect Effects 0.000 description 1
229960005323 phenoxyethanol Drugs 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
210000005238 principal cell Anatomy 0.000 description 1
230000008569 process Effects 0.000 description 1
210000004765 promyelocyte Anatomy 0.000 description 1
239000012521 purified sample Substances 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000000979 retarding effect Effects 0.000 description 1
238000012216 screening Methods 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000004094 surface-active agent Substances 0.000 description 1
238000012800 visualization Methods 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA000465664A 1984-10-17 1984-10-17 Methode volumetrique pour l'etablissement de la formule leucocytaire Expired CA1247995A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA000465664A CA1247995A (fr)	1984-10-17	1984-10-17	Methode volumetrique pour l'etablissement de la formule leucocytaire

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA000465664A CA1247995A (fr)	1984-10-17	1984-10-17	Methode volumetrique pour l'etablissement de la formule leucocytaire

Publications (1)

Publication Number	Publication Date
CA1247995A true CA1247995A (fr)	1989-01-03

Family

ID=4128941

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000465664A Expired CA1247995A (fr)	1984-10-17	1984-10-17	Methode volumetrique pour l'etablissement de la formule leucocytaire

Country Status (1)

Country	Link
CA (1)	CA1247995A (fr)

1984
- 1984-10-17 CA CA000465664A patent/CA1247995A/fr not_active Expired

Legal Events

Date	Code	Title	Description
2006-01-03	MKEX	Expiry

Publication	Publication Date	Title
US4485175A (en)	1984-11-27	Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
EP0144339B1 (fr)	1992-06-24	Procede et reactif de stromatolyse pour la determination volumetrique de populations de leucocytes
EP0185048B1 (fr)	1993-01-27	Procede et systeme reactifs pour la determination differentielle de quatre populations de leucocytes
US4346018A (en)	1982-08-24	Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
EP1004880B1 (fr)	2007-11-14	Methode et réactifs de cytométrie de flux diagnostiques des érythroblastes
US4521518A (en)	1985-06-04	Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US5116539A (en)	1992-05-26	Reagent and method for measuring leukocytes and hemoglobin in blood
JP3048260B2 (ja)	2000-06-05	白血球分類計数用試料調製方法
US6114173A (en)	2000-09-05	Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
EP0268766B1 (fr)	1993-06-16	Procédé pour classifier des leucocytes par cytométrie à écoulement et des réactifs appliqués
US20040241769A1 (en)	2004-12-02	Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
US6632676B1 (en)	2003-10-14	Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
US7674622B2 (en)	2010-03-09	Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer
EP2166353B1 (fr)	2018-08-08	Réactif et trousse de réactifs pour analyse de leucocyte primitif
US4962038A (en)	1990-10-09	Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
JPH07113632B2 (ja)	1995-12-06	白血球分析方法
CA1247995A (fr)	1989-01-03	Methode volumetrique pour l'etablissement de la formule leucocytaire
EP4492038A1 (fr)	2025-01-15	Procédé d'analyse de cellules sanguines et réactif pour analyse de cellules sanguines
CA1243589A (fr)	1988-10-25	Diluant du sang polyvalent et agent lysogene pour le dosage differentiel de la population de leucocytes lymphoides-myeloides