CA1284959C - Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures - Google Patents

Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures

Info

Publication number: CA1284959C
Authority: CA; Canada
Prior art keywords: dna; sequence; phe; promoter region; polypeptide
Prior art date: 1984-12-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA000498511A

Other languages

English (en)

Inventor

Nigel A. Skipper

Martin Sumner-Smith

Roger W. Davies

Richard P. Bozzato

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Allelix Biopharmaceuticals Inc

Original Assignee

Allelix Biopharmaceuticals Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-12-24

Filing date

1985-12-23

Publication date

1991-06-18

1985-12-23 Application filed by Allelix Biopharmaceuticals Inc filed Critical Allelix Biopharmaceuticals Inc

1991-06-18 Application granted granted Critical

1991-06-18 Publication of CA1284959C publication Critical patent/CA1284959C/fr

2008-06-18 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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230000017854 proteolysis Effects 0.000 description 1
238000012207 quantitative assay Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
230000007017 scission Effects 0.000 description 1
230000009962 secretion pathway Effects 0.000 description 1
230000011664 signaling Effects 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
244000000000 soil microbiome Species 0.000 description 1
239000000243 solution Substances 0.000 description 1
238000010186 staining Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
230000002459 sustained effect Effects 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
238000012795 verification Methods 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
239000002023 wood Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Mycology (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Sink And Installation For Waste Water (AREA)
Enzymes And Modification Thereof (AREA)

CA000498511A 1984-12-24 1985-12-23 Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures Expired - Fee Related CA1284959C (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US68570484A	1984-12-24	1984-12-24
US685,704		1984-12-24

Publications (1)

Publication Number	Publication Date
CA1284959C true CA1284959C (fr)	1991-06-18

Family

ID=24753341

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
CA000494013A Expired CA1252256A (fr)	1984-12-24	1985-10-28	Raccord de vidage a contre-ecrou et pieces de branchement en resine synthetique
CA000498511A Expired - Fee Related CA1284959C (fr)	1984-12-24	1985-12-23	Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
CA000494013A Expired CA1252256A (fr)	1984-12-24	1985-10-28	Raccord de vidage a contre-ecrou et pieces de branchement en resine synthetique

Country Status (6)

Country	Link
EP (1)	EP0208706A1 (fr)
JP (1)	JPS62502025A (fr)
AU (1)	AU5304286A (fr)
CA (2)	CA1252256A (fr)
DK (1)	DK403186A (fr)
WO (1)	WO1986003777A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5068185A (en) *	1986-07-10	1991-11-26	Merck & Co., Inc.	Trains of yeast for the expression of heterologous genes
US5055401A (en) *	1987-04-10	1991-10-08	Alko Ltd.	Construction of new α-galactosidase producing yeast strains and the industrial application of these strains
FR2615527B1 (fr) *	1987-05-22	1989-08-18	Lesaffre Soc Ind	Procede d'integration d'une sequence connue d'adn dans les levures ascosporogenes, vecteurs mis en oeuvre et nouvelles souches de levures
DK38893D0 (da)	1993-03-31	1993-03-31	Novo Nordisk As	Dna
JPH08322575A (ja) *	1995-05-30	1996-12-10	Takara Shuzo Co Ltd	プロモーター
ES2351296B8 (es) *	2009-04-08	2012-07-03	Universidade Da Coruña	Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa.
NZ727213A (en)	2012-03-09	2020-03-27	Vestaron Corp	Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11692016B2 (en) *	2012-03-09	2023-07-04	Vestaron Corporation	High gene expression yeast strain
DK2907393T3 (en)	2014-02-17	2018-11-12	Dmk Deutsches Milchkontor Gmbh	Lactose-free milk products
CN110982721B (zh) *	2019-12-09	2022-04-26	宜昌东阳光生化制药有限公司	提高酿酒酵母代谢产物产量的方法

1985
- 1985-10-28 CA CA000494013A patent/CA1252256A/fr not_active Expired
- 1985-12-23 WO PCT/GB1985/000598 patent/WO1986003777A1/fr not_active Ceased
- 1985-12-23 CA CA000498511A patent/CA1284959C/fr not_active Expired - Fee Related
- 1985-12-23 AU AU53042/86A patent/AU5304286A/en not_active Abandoned
- 1985-12-23 EP EP19860900218 patent/EP0208706A1/fr not_active Withdrawn
- 1985-12-23 JP JP61500434A patent/JPS62502025A/ja active Pending
1986
- 1986-08-22 DK DK403186A patent/DK403186A/da not_active Application Discontinuation

Also Published As

Publication number	Publication date
AU5304286A (en)	1986-07-22
EP0208706A1 (fr)	1987-01-21
DK403186D0 (da)	1986-08-22
JPS62502025A (ja)	1987-08-13
DK403186A (da)	1986-08-22
WO1986003777A1 (fr)	1986-07-03
CA1252256A (fr)	1989-04-11

Legal Events

Date	Code	Title	Description
1993-12-20	MKLA	Lapsed

Publication	Publication Date	Title
US5728547A (en)	1998-03-17	Vectors for use in filamentous fungi
EP0284603B1 (fr)	1998-01-07	Utilisation d'activeurs de champignons filamenteux
AU567031B2 (en)	1987-11-05	Glucoamylase cdna
US5393670A (en)	1995-02-28	DNA, vectors and transformed hosts encoding Trichoderma reesei endoglucanase I
CA1340547C (fr)	1999-05-18	Nouvelles sequences-guides de secretion pour levures
EP0301669B1 (fr)	1993-06-16	Constructions d'ADN contenant une séquence leader de facteur alpha de Kluyveromyces pour diriger la sécrétion de polypeptides hétérologues
EP0749478A1 (fr)	1996-12-27	Souches de levure et albumines modifiees
HU218731B (hu)	2000-11-28	Élesztőtörzs és eljárás az élesztőre heterológ fehérjék előállítására
CA1284959C (fr)	1991-06-18	Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures
Roca et al.	1996	Cloning of the Penicillium minioluteum gene encoding dextranase and its expression in Pichia pastoris
Goto et al.	1990	Cloning and nucleotide sequence of the KHR killer gene of Saccharomyces cerevisiae
US5627046A (en)	1997-05-06	Yeast promoter and its use
IE72506B1 (en)	1997-04-23	Novel expression system
CA1338859C (fr)	1997-01-21	Systeme d'expression
RU2091490C1 (ru)	1997-09-27	Способ получения гетерологичного полипептида в эукариотических микроорганизмов
US5834191A (en)	1998-11-10	Production of heterologous peptides
US5521093A (en)	1996-05-28	Yeast vector coding for heterologous gene fusions linked via KEX2 cleavage site and coding for truncated KEX2 genes
EP0362183B1 (fr)	1993-06-16	Procédé de production de lysozyme humain
CA2069962A1 (fr)	1992-02-18	Production de beta-1,3 glucanase a l'aide d'adn recombinant
US5447862A (en)	1995-09-05	Pectin lyase genes of aspergillus niger
EP1031628A1 (fr)	2000-08-30	Méthode de production d'inhibiteurs de protéase
US5879926A (en)	1999-03-09	Yeast strains for the production of mature heterologous proteins, especially hirudin
Gwynne	1992	Foreign proteins
Hatano et al.	1994	Expression of the carboxymethylcellulase gene, CMC1, from Cryptococcus flavus in Saccharomyces cerevisiae
JPH0391486A (ja)	1991-04-17	新規遺伝子、ベクター、それを用いた形質転換体、及びその利用