CA1295560C - Sonde d'adn telomerique - Google Patents
Sonde d'adn telomeriqueInfo
- Publication number
- CA1295560C CA1295560C CA000526211A CA526211A CA1295560C CA 1295560 C CA1295560 C CA 1295560C CA 000526211 A CA000526211 A CA 000526211A CA 526211 A CA526211 A CA 526211A CA 1295560 C CA1295560 C CA 1295560C
- Authority
- CA
- Canada
- Prior art keywords
- dna
- fragments
- probe
- fragment
- individuals
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000523 sample Substances 0.000 title claims abstract description 47
- 210000002593 Y chromosome Anatomy 0.000 claims abstract description 20
- 239000003298 DNA probe Substances 0.000 claims abstract description 16
- 210000001766 X chromosome Anatomy 0.000 claims abstract description 14
- 108020003215 DNA Probes Proteins 0.000 claims abstract description 12
- 239000012634 fragment Substances 0.000 claims description 77
- 230000002068 genetic effect Effects 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- 108091008146 restriction endonucleases Proteins 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 241001131785 Escherichia coli HB101 Species 0.000 claims description 2
- 238000009877 rendering Methods 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 230000007170 pathology Effects 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 55
- 230000006798 recombination Effects 0.000 description 10
- 238000005215 recombination Methods 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 8
- 238000001962 electrophoresis Methods 0.000 description 7
- 230000008775 paternal effect Effects 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 102000054765 polymorphisms of proteins Human genes 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 102000008482 12E7 Antigen Human genes 0.000 description 4
- 108010020567 12E7 Antigen Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 238000011835 investigation Methods 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000003765 sex chromosome Anatomy 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000017105 transposition Effects 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 108091092919 Minisatellite Proteins 0.000 description 2
- 101500006448 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) Endonuclease PI-MboI Proteins 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 102000054766 genetic haplotypes Human genes 0.000 description 2
- 210000004090 human X chromosome Anatomy 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 108010052418 (N-(2-((4-((2-((4-(9-acridinylamino)phenyl)amino)-2-oxoethyl)amino)-4-oxobutyl)amino)-1-(1H-imidazol-4-ylmethyl)-1-oxoethyl)-6-(((-2-aminoethyl)amino)methyl)-2-pyridinecarboxamidato) iron(1+) Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 108091060290 Chromatid Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 208000031220 Hemophilia Diseases 0.000 description 1
- 208000009292 Hemophilia A Diseases 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- OAICVXFJPJFONN-OUBTZVSYSA-N Phosphorus-32 Chemical compound [32P] OAICVXFJPJFONN-OUBTZVSYSA-N 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 208000007014 Retinitis pigmentosa Diseases 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000002457 bidirectional effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004756 chromatid Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000013601 cosmid vector Substances 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 210000001182 human Y chromosome Anatomy 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229940097886 phosphorus 32 Drugs 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 108091035539 telomere Proteins 0.000 description 1
- 102000055501 telomere Human genes 0.000 description 1
- 210000003411 telomere Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92252286A | 1986-10-24 | 1986-10-24 | |
| US922,522 | 1986-10-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1295560C true CA1295560C (fr) | 1992-02-11 |
Family
ID=25447157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000526211A Expired - Lifetime CA1295560C (fr) | 1986-10-24 | 1986-12-23 | Sonde d'adn telomerique |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1295560C (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
-
1986
- 1986-12-23 CA CA000526211A patent/CA1295560C/fr not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5698686A (en) * | 1994-10-20 | 1997-12-16 | Arch Development Corporation | Yeast telomerase compositions |
| US5916752A (en) * | 1994-10-20 | 1999-06-29 | Arch Development Corporation | Telomerase screening methods |
| US6387619B1 (en) | 1994-10-20 | 2002-05-14 | Arch Development | Telomerase compositions and methods |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed | ||
| MKLA | Lapsed |
Effective date: 19950812 |