CA1296257C - Colonne de chromatographie - Google Patents
Colonne de chromatographieInfo
- Publication number
- CA1296257C CA1296257C CA000505906A CA505906A CA1296257C CA 1296257 C CA1296257 C CA 1296257C CA 000505906 A CA000505906 A CA 000505906A CA 505906 A CA505906 A CA 505906A CA 1296257 C CA1296257 C CA 1296257C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- stationary phase
- column
- distribution
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 30
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 156
- 238000009826 distribution Methods 0.000 claims abstract description 65
- 239000011159 matrix material Substances 0.000 claims abstract description 46
- 238000013375 chromatographic separation Methods 0.000 claims abstract description 23
- 125000006850 spacer group Chemical group 0.000 claims description 42
- 239000007787 solid Substances 0.000 claims description 34
- 239000012530 fluid Substances 0.000 claims description 26
- 238000004891 communication Methods 0.000 claims description 21
- 230000008961 swelling Effects 0.000 claims description 12
- 230000002093 peripheral effect Effects 0.000 claims description 9
- 229920001169 thermoplastic Polymers 0.000 claims description 8
- 230000002708 enhancing effect Effects 0.000 claims description 7
- 230000005465 channeling Effects 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 238000013022 venting Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 description 64
- 239000010410 layer Substances 0.000 description 34
- 239000012501 chromatography medium Substances 0.000 description 33
- 239000000463 material Substances 0.000 description 31
- 108091006146 Channels Proteins 0.000 description 29
- 238000000926 separation method Methods 0.000 description 18
- 239000012071 phase Substances 0.000 description 13
- 239000012528 membrane Substances 0.000 description 11
- 210000004379 membrane Anatomy 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 238000012856 packing Methods 0.000 description 9
- 239000000835 fiber Substances 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 7
- 239000004416 thermosoftening plastic Substances 0.000 description 7
- 239000002131 composite material Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 238000004804 winding Methods 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 125000003636 chemical group Chemical group 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229920001059 synthetic polymer Polymers 0.000 description 4
- 238000001042 affinity chromatography Methods 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 235000015110 jellies Nutrition 0.000 description 3
- 239000008274 jelly Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003466 welding Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004237 preparative chromatography Methods 0.000 description 2
- 230000003405 preventing effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 208000036366 Sensation of pressure Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 238000012435 analytical chromatography Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000012632 extractable Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012527 feed solution Substances 0.000 description 1
- 238000009950 felting Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910021485 fumed silica Inorganic materials 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000012500 ion exchange media Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004460 liquid liquid chromatography Methods 0.000 description 1
- 238000000506 liquid--solid chromatography Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004810 partition chromatography Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 239000012815 thermoplastic material Substances 0.000 description 1
- 229920005992 thermoplastic resin Polymers 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US723,691 | 1985-04-16 | ||
| US06/723,691 US4675104A (en) | 1983-06-17 | 1985-04-16 | Chromatography column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1296257C true CA1296257C (fr) | 1992-02-25 |
Family
ID=24907284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000505906A Expired - Lifetime CA1296257C (fr) | 1985-04-16 | 1986-04-04 | Colonne de chromatographie |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1296257C (fr) |
-
1986
- 1986-04-04 CA CA000505906A patent/CA1296257C/fr not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4675104A (en) | Chromatography column | |
| US4743373A (en) | Chromatography column | |
| US4986909A (en) | Chromatography column | |
| US9072988B2 (en) | Prefilter system for biological systems | |
| US9120037B2 (en) | Stackable planar adsorptive devices | |
| EP0047617B1 (fr) | Colonne de séparation moléculaire et son utilisation | |
| US5911883A (en) | Flow-by solid phase extraction method | |
| EP3033157B1 (fr) | Dispositifs d'adsorption plats empilables | |
| GB2440244A (en) | Chromatography column | |
| US20020166816A1 (en) | Chromatography apparatus and methods | |
| US11219844B2 (en) | Stackable planar adsorptive devices | |
| US5972218A (en) | Porous tube filter | |
| US11845059B2 (en) | Chromatographic cassette | |
| CA1296257C (fr) | Colonne de chromatographie | |
| KR920007851B1 (ko) | 크로마토그래피 분리관 | |
| JP4842449B2 (ja) | クロマトグラフィー用多孔質体及びカラム | |
| CA2047902A1 (fr) | Cartouche de chromatographie | |
| US20240367150A1 (en) | Monolithic microporous substrate for chromatographic beds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |