CA1306208C - Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies - Google Patents

Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies

Info

Publication number
CA1306208C
CA1306208C CA 471799 CA471799A CA1306208C CA 1306208 C CA1306208 C CA 1306208C CA 471799 CA471799 CA 471799 CA 471799 A CA471799 A CA 471799A CA 1306208 C CA1306208 C CA 1306208C
Authority
CA
Canada
Prior art keywords
sequence
polypeptide
dna sequence
codes
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 471799
Other languages
English (en)
Inventor
Ray J. Wu
Saran A. Narang
Wing L. Sung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council Canada
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 471799 priority Critical patent/CA1306208C/fr
Application granted granted Critical
Publication of CA1306208C publication Critical patent/CA1306208C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA 471799 1985-01-09 1985-01-09 Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies Expired - Lifetime CA1306208C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 471799 CA1306208C (fr) 1985-01-09 1985-01-09 Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 471799 CA1306208C (fr) 1985-01-09 1985-01-09 Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies

Publications (1)

Publication Number Publication Date
CA1306208C true CA1306208C (fr) 1992-08-11

Family

ID=4129550

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 471799 Expired - Lifetime CA1306208C (fr) 1985-01-09 1985-01-09 Augmentation de l'expression et de la stabilisation des produits genetiquesdans les microorganismes a l'aide de courtes sequences-signaux choisies

Country Status (1)

Country Link
CA (1) CA1306208C (fr)

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