CA1339794C - Determination enzymatique de la theophylline - Google Patents
Determination enzymatique de la theophyllineInfo
- Publication number
- CA1339794C CA1339794C CA000590650A CA590650A CA1339794C CA 1339794 C CA1339794 C CA 1339794C CA 000590650 A CA000590650 A CA 000590650A CA 590650 A CA590650 A CA 590650A CA 1339794 C CA1339794 C CA 1339794C
- Authority
- CA
- Canada
- Prior art keywords
- theophylline
- sample
- signal
- enzyme
- test composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 title claims abstract description 703
- 229960000278 theophylline Drugs 0.000 title claims abstract description 350
- 230000002255 enzymatic effect Effects 0.000 title abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 160
- 108090000790 Enzymes Proteins 0.000 claims abstract description 160
- 238000000034 method Methods 0.000 claims abstract description 96
- 239000000203 mixture Substances 0.000 claims abstract description 90
- 238000012360 testing method Methods 0.000 claims abstract description 78
- 210000001124 body fluid Anatomy 0.000 claims abstract description 4
- 239000010839 body fluid Substances 0.000 claims abstract description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 64
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 46
- 101710088194 Dehydrogenase Proteins 0.000 claims description 43
- 230000008859 change Effects 0.000 claims description 40
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 36
- 108010075031 Cytochromes c Proteins 0.000 claims description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims description 26
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical group C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 26
- OTSBKHHWSQYEHK-UHFFFAOYSA-N 1,3-dimethyluric acid Chemical group O=C1N(C)C(=O)N(C)C2=C1NC(=O)N2 OTSBKHHWSQYEHK-UHFFFAOYSA-N 0.000 claims description 25
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims description 25
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 claims description 25
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- 108010007793 theophylline oxidase Proteins 0.000 claims description 22
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- 238000005259 measurement Methods 0.000 claims description 18
- -1 potassium ferricyanide Chemical group 0.000 claims description 17
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- 229950006238 nadide Drugs 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 11
- 239000007795 chemical reaction product Substances 0.000 claims description 11
- 239000001301 oxygen Substances 0.000 claims description 11
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- 229940075420 xanthine Drugs 0.000 claims description 11
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- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 claims description 8
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 claims description 7
- YPZRHBJKEMOYQH-UYBVJOGSSA-N FADH2 Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C(NC(=O)NC2=O)=C2NC2=C1C=C(C)C(C)=C2 YPZRHBJKEMOYQH-UYBVJOGSSA-N 0.000 claims description 6
- 238000002848 electrochemical method Methods 0.000 claims description 6
- 108010093894 Xanthine oxidase Proteins 0.000 claims description 5
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims description 4
- 102100033220 Xanthine oxidase Human genes 0.000 claims description 4
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- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims 3
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- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 18
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- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 14
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- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 10
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- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 6
- PFWLFWPASULGAN-UHFFFAOYSA-N 7-Methylxanthine Natural products N1C(=O)NC(=O)C2=C1N=CN2C PFWLFWPASULGAN-UHFFFAOYSA-N 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
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- DHDHJYNTEFLIHY-UHFFFAOYSA-N 4,7-diphenyl-1,10-phenanthroline Chemical compound C1=CC=CC=C1C1=CC=NC2=C1C=CC1=C(C=3C=CC=CC=3)C=CN=C21 DHDHJYNTEFLIHY-UHFFFAOYSA-N 0.000 description 3
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Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15549888A | 1988-02-12 | 1988-02-12 | |
| US155,498 | 1988-02-12 | ||
| US57220390A | 1990-08-23 | 1990-08-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1339794C true CA1339794C (fr) | 1998-04-07 |
Family
ID=26852363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000590650A Expired - Fee Related CA1339794C (fr) | 1988-02-12 | 1989-02-09 | Determination enzymatique de la theophylline |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA1339794C (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11834697B2 (en) | 2017-09-15 | 2023-12-05 | Oxford University Innovation Limited | Electrochemical recognition and quantification of cytochrome c oxidase expression in bacteria |
-
1989
- 1989-02-09 CA CA000590650A patent/CA1339794C/fr not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11834697B2 (en) | 2017-09-15 | 2023-12-05 | Oxford University Innovation Limited | Electrochemical recognition and quantification of cytochrome c oxidase expression in bacteria |
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