CA1340661C - Virus syncytial respiratoire: vaccins et essais diagnostics - Google Patents

Virus syncytial respiratoire: vaccins et essais diagnostics

Info

Publication number: CA1340661C
Authority: CA; Canada
Prior art keywords: virus; protein; fusion protein; fusion; animals
Prior art date: 1987-09-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA000578669A

Other languages

English (en)

Inventor

Peter R. Paradiso

Stephen W. Hildreth

Branda T. Hu

Antonia Martin-Gallardo

Rasappa Arumugham

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Wyeth Holdings LLC

Original Assignee

HU BRADELY W

American Cyanamid Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-09-29

Filing date

1988-09-28

Publication date

1999-07-20

1988-09-28 Application filed by HU BRADELY W, American Cyanamid Co filed Critical HU BRADELY W

1999-07-20 Application granted granted Critical

1999-07-20 Publication of CA1340661C publication Critical patent/CA1340661C/fr

2016-07-20 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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CA000578669A 1987-09-29 1988-09-28 Virus syncytial respiratoire: vaccins et essais diagnostics Expired - Fee Related CA1340661C (fr)

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US10218087A	1987-09-29	1987-09-29
US07/102,180		1987-09-29

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Publication	Publication Date	Title
US5223254A (en)	1993-06-29	Respiratory syncytial virus: vaccines
US5639853A (en)	1997-06-17	Respiratory syncytial virus vaccines
US5294548A (en)	1994-03-15	Recombianant Hepatitis a virus
EP0832253B1 (fr)	2004-05-19	Vaccins a acides nucleiques du virus respiratoire syncytial
AU778144B2 (en)	2004-11-18	Nucleic acid respiratory syncytial virus vaccines
Wathen et al.	1989	Immunization of cotton rats with the human respiratory syncytial virus F glycoprotein produced using a baculovirus vector
EP0573614A1 (fr)	1993-12-15	Virus de la pseudorage s-prv-155 attenue et obtenu par genie genetique, et ses utilisations
JPH08253428A (ja)	1996-10-01	膜結合性タンパク質を含有するワクチン類
US7645455B2 (en)	2010-01-12	Chimeric lyssavirus nucleic acids and polypeptides
US5874279A (en)	1999-02-23	Recombinant infectious bovine rhinotracheitis virus
EP0498804B1 (fr)	1997-03-05	Procede de preparation d'un vaccin ou d'un agent toxique
AU756222B2 (en)	2003-01-09	Nucleic acid vaccines encoding G protein of respiratory syncytial virus
US7264817B1 (en)	2007-09-04	Immunogenic composition based on a truncated derivative of a membrane bound protein and process for making it
CA1340661C (fr)	1999-07-20	Virus syncytial respiratoire: vaccins et essais diagnostics
CA1336955C (fr)	1995-09-12	Virus respiratoire syncitial: vaccins et essais diagnostiques
US7238672B1 (en)	2007-07-03	Chimeric lyssavirus nucleic acids and polypeptides
WO1996016184A1 (fr)	1996-05-30	Expression de la glycoproteine hemagglutinine/neuraminidase (hn) du virus parainfluenza bovin du type 3 dans deux systemes heterologues
PT1721982E (pt)	2013-06-06	Ácidos nucleicos e polipéptidos quiméricos de lissavírus
Walravens et al.	1996	Immunological response of mice to the bovine respiratory syncytial virus fusion glycoprotein expressed in recombinant baculovirus infected insect cells
Ahmad	1992	Expression of lymphokine and viral genes by recombinant baculovirus in insect cells and larvae for development of adjuvant proteins, subunit vaccines, and rapid diagnostic kits
MXPA00000636A (en)	2001-11-21	Nucleic acid vaccines encoding g protein of respiratory syncytial virus