CA1340761C - Recepteurs d'interleukine 4 - Google Patents

Info

Publication number

CA1340761C

CA1340761C CA 614293 CA614293A CA1340761C CA 1340761 C CA1340761 C CA 1340761C CA 614293 CA614293 CA 614293 CA 614293 A CA614293 A CA 614293A CA 1340761 C CA1340761 C CA 1340761C

Authority

CA

Canada

Prior art keywords

receptor

human

amino acid

figures

sequence

Prior art date

1989-03-20

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired - Fee Related

Links

• Espacenet
• Global Dossier
• CIPO
• Discuss

• 108010038486 Interleukin-4 Receptors Proteins 0.000 title claims abstract description 213
• 102000010787 Interleukin-4 Receptors Human genes 0.000 title claims abstract description 212
• 108020004414 DNA Proteins 0.000 claims abstract description 39
• 238000000034 method Methods 0.000 claims abstract description 32
• 239000013604 expression vector Substances 0.000 claims abstract description 24
• 230000008569 process Effects 0.000 claims abstract description 7
• 210000004027 cell Anatomy 0.000 claims description 187
• 108090000623 proteins and genes Proteins 0.000 claims description 100
• 102000004169 proteins and genes Human genes 0.000 claims description 73
• 239000002299 complementary DNA Substances 0.000 claims description 70
• 102000004388 Interleukin-4 Human genes 0.000 claims description 69
• 108090000978 Interleukin-4 Proteins 0.000 claims description 69
• 102000005962 receptors Human genes 0.000 claims description 66
• 108020003175 receptors Proteins 0.000 claims description 66
• 230000027455 binding Effects 0.000 claims description 54
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 50
• 239000002773 nucleotide Substances 0.000 claims description 45
• 125000003729 nucleotide group Chemical group 0.000 claims description 45
• 150000001413 amino acids Chemical class 0.000 claims description 42
• 230000014509 gene expression Effects 0.000 claims description 42
• 241001529936 Murinae Species 0.000 claims description 41
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 34
• 239000012634 fragment Substances 0.000 claims description 34
• 101001002709 Homo sapiens Interleukin-4 Proteins 0.000 claims description 29
• 102000055229 human IL4 Human genes 0.000 claims description 29
• 239000013598 vector Substances 0.000 claims description 28
• 108020004999 messenger RNA Proteins 0.000 claims description 27
• 241000282414 Homo sapiens Species 0.000 claims description 25
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 21
• 239000000203 mixture Substances 0.000 claims description 20
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 17
• 229920001184 polypeptide Polymers 0.000 claims description 16
• 230000000694 effects Effects 0.000 claims description 13
• 230000001105 regulatory effect Effects 0.000 claims description 12
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 12
• 125000000539 amino acid group Chemical group 0.000 claims description 11
• 230000000813 microbial effect Effects 0.000 claims description 10
• 238000000159 protein binding assay Methods 0.000 claims description 10
• 230000002103 transcriptional effect Effects 0.000 claims description 10
• 238000013518 transcription Methods 0.000 claims description 9
• 238000009396 hybridization Methods 0.000 claims description 8
• 230000035897 transcription Effects 0.000 claims description 8
• 230000003612 virological effect Effects 0.000 claims description 8
• 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 7
• 230000002068 genetic effect Effects 0.000 claims description 7
• 230000013595 glycosylation Effects 0.000 claims description 7
• 238000006206 glycosylation reaction Methods 0.000 claims description 7
• 238000003259 recombinant expression Methods 0.000 claims description 7
• 102000003886 Glycoproteins Human genes 0.000 claims description 6
• 108090000288 Glycoproteins Proteins 0.000 claims description 6
• 230000028993 immune response Effects 0.000 claims description 6
• 230000004044 response Effects 0.000 claims description 6
• 239000000463 material Substances 0.000 claims description 5
• 241000124008 Mammalia Species 0.000 claims description 4
• 230000002163 immunogen Effects 0.000 claims description 4
• 238000013519 translation Methods 0.000 claims description 4
• 101001033312 Homo sapiens Interleukin-4 receptor subunit alpha Proteins 0.000 claims description 3
• 108700001097 Insect Genes Proteins 0.000 claims description 3
• 108700005443 Microbial Genes Proteins 0.000 claims description 3
• 108700005077 Viral Genes Proteins 0.000 claims description 3
• 208000006673 asthma Diseases 0.000 claims description 3
• 239000003085 diluting agent Substances 0.000 claims description 3
• 102000054663 human IL4R Human genes 0.000 claims description 3
• 238000004519 manufacturing process Methods 0.000 claims description 3
• 210000003705 ribosome Anatomy 0.000 claims description 3
• 230000009870 specific binding Effects 0.000 claims description 3
• 238000012258 culturing Methods 0.000 claims description 2
• 230000001086 cytosolic effect Effects 0.000 claims description 2
• 239000003814 drug Substances 0.000 claims description 2
• 239000003937 drug carrier Substances 0.000 claims description 2
• 229940028885 interleukin-4 Drugs 0.000 claims description 2
• 208000026935 allergic disease Diseases 0.000 claims 1
• 230000008512 biological response Effects 0.000 claims 1
• 230000002401 inhibitory effect Effects 0.000 claims 1
• 230000003993 interaction Effects 0.000 claims 1
• 239000008194 pharmaceutical composition Substances 0.000 claims 1
• 230000001737 promoting effect Effects 0.000 claims 1
• 238000004113 cell culture Methods 0.000 abstract description 7
• 235000018102 proteins Nutrition 0.000 description 63
• 229940024606 amino acid Drugs 0.000 description 45
• 235000001014 amino acid Nutrition 0.000 description 43
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 32
• 239000013612 plasmid Substances 0.000 description 18
• 238000000746 purification Methods 0.000 description 17
• 230000010076 replication Effects 0.000 description 17
• 239000000523 sample Substances 0.000 description 17
• 239000000499 gel Substances 0.000 description 15
• 239000006228 supernatant Substances 0.000 description 15
• 241000588724 Escherichia coli Species 0.000 description 14
• 238000004458 analytical method Methods 0.000 description 14
• 108091026890 Coding region Proteins 0.000 description 13
• 238000003556 assay Methods 0.000 description 13
• 239000002953 phosphate buffered saline Substances 0.000 description 12
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 11
• 241000699670 Mus sp. Species 0.000 description 11
• 238000010367 cloning Methods 0.000 description 11
• 210000003719 b-lymphocyte Anatomy 0.000 description 10
• 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 9
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 9
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 9
• 150000007523 nucleic acids Chemical group 0.000 description 9
• 108020004705 Codon Proteins 0.000 description 8
• 108020004635 Complementary DNA Proteins 0.000 description 8
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 8
• 108700026244 Open Reading Frames Proteins 0.000 description 8
• 108010076504 Protein Sorting Signals Proteins 0.000 description 8
• 210000001744 T-lymphocyte Anatomy 0.000 description 8
• 230000004071 biological effect Effects 0.000 description 8
• 230000001580 bacterial effect Effects 0.000 description 7
• 239000012528 membrane Substances 0.000 description 7
• 210000004379 membrane Anatomy 0.000 description 7
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 7
• 230000028327 secretion Effects 0.000 description 7
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
• 108010002350 Interleukin-2 Proteins 0.000 description 6
• 102000000588 Interleukin-2 Human genes 0.000 description 6
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
• 108020005038 Terminator Codon Proteins 0.000 description 6
• 239000002253 acid Substances 0.000 description 6
• 150000007513 acids Chemical class 0.000 description 6
• 238000012217 deletion Methods 0.000 description 6
• 230000037430 deletion Effects 0.000 description 6
• 239000003623 enhancer Substances 0.000 description 6
• 239000000284 extract Substances 0.000 description 6
• 239000003550 marker Substances 0.000 description 6
• 239000002609 medium Substances 0.000 description 6
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 6
• 238000002360 preparation method Methods 0.000 description 6
• 238000006467 substitution reaction Methods 0.000 description 6
• GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 6
• 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 5
• 101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 5
• 241000894006 Bacteria Species 0.000 description 5
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
• -1 M-maleimidobenzoyl succinimide ester Chemical class 0.000 description 5
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
• 238000007792 addition Methods 0.000 description 5
• 230000015572 biosynthetic process Effects 0.000 description 5
• 239000003153 chemical reaction reagent Substances 0.000 description 5
• 238000004132 cross linking Methods 0.000 description 5
• 239000008103 glucose Substances 0.000 description 5
• 238000004128 high performance liquid chromatography Methods 0.000 description 5
• 238000003780 insertion Methods 0.000 description 5
• 230000037431 insertion Effects 0.000 description 5
• 150000002500 ions Chemical class 0.000 description 5
• 239000011159 matrix material Substances 0.000 description 5
• 230000035772 mutation Effects 0.000 description 5
• 230000008488 polyadenylation Effects 0.000 description 5
• 239000000047 product Substances 0.000 description 5
• 230000035755 proliferation Effects 0.000 description 5
• 238000004007 reversed phase HPLC Methods 0.000 description 5
• 238000012163 sequencing technique Methods 0.000 description 5
• 210000004989 spleen cell Anatomy 0.000 description 5
• 238000001890 transfection Methods 0.000 description 5
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
• 101100069857 Caenorhabditis elegans hil-4 gene Proteins 0.000 description 4
• 241000282693 Cercopithecidae Species 0.000 description 4
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
• 241000701109 Human adenovirus 2 Species 0.000 description 4
• 108091034117 Oligonucleotide Proteins 0.000 description 4
• 206010035148 Plague Diseases 0.000 description 4
• PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
• 108010022394 Threonine synthase Proteins 0.000 description 4
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
• 241000607479 Yersinia pestis Species 0.000 description 4
• 238000001042 affinity chromatography Methods 0.000 description 4
• 238000001261 affinity purification Methods 0.000 description 4
• 229960000723 ampicillin Drugs 0.000 description 4
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
• 210000000170 cell membrane Anatomy 0.000 description 4
• 210000001072 colon Anatomy 0.000 description 4
• 239000003599 detergent Substances 0.000 description 4
• 102000004419 dihydrofolate reductase Human genes 0.000 description 4
• 230000004927 fusion Effects 0.000 description 4
• 210000004408 hybridoma Anatomy 0.000 description 4
• 238000000338 in vitro Methods 0.000 description 4
• 230000005764 inhibitory process Effects 0.000 description 4
• 210000001165 lymph node Anatomy 0.000 description 4
• 238000003786 synthesis reaction Methods 0.000 description 4
• 238000002560 therapeutic procedure Methods 0.000 description 4
• 230000009466 transformation Effects 0.000 description 4
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
• GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 3
• WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
• 229930024421 Adenine Natural products 0.000 description 3
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
• 102000053602 DNA Human genes 0.000 description 3
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
• 102000004190 Enzymes Human genes 0.000 description 3
• 108090000790 Enzymes Proteins 0.000 description 3
• 241000701024 Human betaherpesvirus 5 Species 0.000 description 3
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
• 241001465754 Metazoa Species 0.000 description 3
• 239000000020 Nitrocellulose Substances 0.000 description 3
• 239000002033 PVDF binder Substances 0.000 description 3
• 238000012300 Sequence Analysis Methods 0.000 description 3
• BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
• 239000007983 Tris buffer Substances 0.000 description 3
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
• 229960000643 adenine Drugs 0.000 description 3
• 230000000735 allogeneic effect Effects 0.000 description 3
• 239000000427 antigen Substances 0.000 description 3
• 108091007433 antigens Proteins 0.000 description 3
• 102000036639 antigens Human genes 0.000 description 3
• 210000004899 c-terminal region Anatomy 0.000 description 3
• 150000001720 carbohydrates Chemical class 0.000 description 3
• 235000014633 carbohydrates Nutrition 0.000 description 3
• 239000000969 carrier Substances 0.000 description 3
• 238000005119 centrifugation Methods 0.000 description 3
• 239000003795 chemical substances by application Substances 0.000 description 3
• 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
• 239000013599 cloning vector Substances 0.000 description 3
• 239000002131 composite material Substances 0.000 description 3
• 239000003636 conditioned culture medium Substances 0.000 description 3
• ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 238000003745 diagnosis Methods 0.000 description 3
• 238000010828 elution Methods 0.000 description 3
• 230000001747 exhibiting effect Effects 0.000 description 3
• 239000013613 expression plasmid Substances 0.000 description 3
• 238000000605 extraction Methods 0.000 description 3
• 239000001963 growth medium Substances 0.000 description 3
• 238000001727 in vivo Methods 0.000 description 3
• 210000004962 mammalian cell Anatomy 0.000 description 3
• 238000002703 mutagenesis Methods 0.000 description 3
• 231100000350 mutagenesis Toxicity 0.000 description 3
• 229920001220 nitrocellulos Polymers 0.000 description 3
• 108020004707 nucleic acids Proteins 0.000 description 3
• 102000039446 nucleic acids Human genes 0.000 description 3
• 239000008188 pellet Substances 0.000 description 3
• 229920002401 polyacrylamide Polymers 0.000 description 3
• 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
• 238000010839 reverse transcription Methods 0.000 description 3
• 238000012216 screening Methods 0.000 description 3
• 229910052709 silver Inorganic materials 0.000 description 3
• 239000004332 silver Substances 0.000 description 3
• 239000011780 sodium chloride Substances 0.000 description 3
• 239000000243 solution Substances 0.000 description 3
• 239000000758 substrate Substances 0.000 description 3
• 238000012360 testing method Methods 0.000 description 3
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
• OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
• 229920000936 Agarose Polymers 0.000 description 2
• 208000002109 Argyria Diseases 0.000 description 2
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
• 101710125089 Bindin Proteins 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
• HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
• 102000004127 Cytokines Human genes 0.000 description 2
• 108090000695 Cytokines Proteins 0.000 description 2
• 238000001712 DNA sequencing Methods 0.000 description 2
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
• XZWYTXMRWQJBGX-VXBMVYAYSA-N FLAG peptide Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=C(O)C=C1 XZWYTXMRWQJBGX-VXBMVYAYSA-N 0.000 description 2
• 241000238631 Hexapoda Species 0.000 description 2
• 101001002657 Homo sapiens Interleukin-2 Proteins 0.000 description 2
• 241000713772 Human immunodeficiency virus 1 Species 0.000 description 2
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
• 102100034343 Integrase Human genes 0.000 description 2
• 108091092195 Intron Proteins 0.000 description 2
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
• 229930193140 Neomycin Natural products 0.000 description 2
• 206010028980 Neoplasm Diseases 0.000 description 2
• 229910019142 PO4 Inorganic materials 0.000 description 2
• 239000001888 Peptone Substances 0.000 description 2
• 108010080698 Peptones Proteins 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 206010035226 Plasma cell myeloma Diseases 0.000 description 2
• 108020005067 RNA Splice Sites Proteins 0.000 description 2
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
• 108020004511 Recombinant DNA Proteins 0.000 description 2
• 229920005654 Sephadex Polymers 0.000 description 2
• 239000012507 Sephadex™ Substances 0.000 description 2
• 108700005078 Synthetic Genes Proteins 0.000 description 2
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
• MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 2
• 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
• 230000001464 adherent effect Effects 0.000 description 2
• 230000003321 amplification Effects 0.000 description 2
• 238000013459 approach Methods 0.000 description 2
• 108010092854 aspartyllysine Proteins 0.000 description 2
• 239000000872 buffer Substances 0.000 description 2
• 201000011510 cancer Diseases 0.000 description 2
• 229940041514 candida albicans extract Drugs 0.000 description 2
• 230000015556 catabolic process Effects 0.000 description 2
• 238000010370 cell cloning Methods 0.000 description 2
• 230000008859 change Effects 0.000 description 2
• 238000012512 characterization method Methods 0.000 description 2
• 238000006243 chemical reaction Methods 0.000 description 2
• 238000003776 cleavage reaction Methods 0.000 description 2
• 230000000295 complement effect Effects 0.000 description 2
• 239000012228 culture supernatant Substances 0.000 description 2
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
• 235000018417 cysteine Nutrition 0.000 description 2
• 229940112382 cysteine / methionine Drugs 0.000 description 2
• 238000006731 degradation reaction Methods 0.000 description 2
• 230000029087 digestion Effects 0.000 description 2
• 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
• 238000005516 engineering process Methods 0.000 description 2
• 238000002474 experimental method Methods 0.000 description 2
• 238000000855 fermentation Methods 0.000 description 2
• 230000004151 fermentation Effects 0.000 description 2
• 239000012091 fetal bovine serum Substances 0.000 description 2
• 239000012467 final product Substances 0.000 description 2
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
• 230000002538 fungal effect Effects 0.000 description 2
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
• 229910052588 hydroxylapatite Inorganic materials 0.000 description 2
• FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
• 238000011534 incubation Methods 0.000 description 2
• 108040006852 interleukin-4 receptor activity proteins Proteins 0.000 description 2
• 238000002955 isolation Methods 0.000 description 2
• 230000001404 mediated effect Effects 0.000 description 2
• 102000006240 membrane receptors Human genes 0.000 description 2
• 229960000485 methotrexate Drugs 0.000 description 2
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
• 244000005700 microbiome Species 0.000 description 2
• 238000010369 molecular cloning Methods 0.000 description 2
• 201000000050 myeloid neoplasm Diseases 0.000 description 2
• 230000010807 negative regulation of binding Effects 0.000 description 2
• 229960004927 neomycin Drugs 0.000 description 2
• 230000009871 nonspecific binding Effects 0.000 description 2
• 238000003199 nucleic acid amplification method Methods 0.000 description 2
• XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 2
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
• 235000019319 peptone Nutrition 0.000 description 2
• 239000010452 phosphate Substances 0.000 description 2
• 239000008363 phosphate buffer Substances 0.000 description 2
• 239000013600 plasmid vector Substances 0.000 description 2
• 239000002243 precursor Substances 0.000 description 2
• 230000009465 prokaryotic expression Effects 0.000 description 2
• 238000011084 recovery Methods 0.000 description 2
• 230000007017 scission Effects 0.000 description 2
• 230000003248 secreting effect Effects 0.000 description 2
• 239000006152 selective media Substances 0.000 description 2
• 238000000926 separation method Methods 0.000 description 2
• 210000002966 serum Anatomy 0.000 description 2
• 239000011734 sodium Substances 0.000 description 2
• 229910052708 sodium Inorganic materials 0.000 description 2
• 239000001488 sodium phosphate Substances 0.000 description 2
• 229910000162 sodium phosphate Inorganic materials 0.000 description 2
• 239000007790 solid phase Substances 0.000 description 2
• 241000894007 species Species 0.000 description 2
• 238000010186 staining Methods 0.000 description 2
• 230000000638 stimulation Effects 0.000 description 2
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
• 239000000126 substance Substances 0.000 description 2
• 230000002194 synthesizing effect Effects 0.000 description 2
• 210000001519 tissue Anatomy 0.000 description 2
• RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
• 229940035893 uracil Drugs 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• 210000005253 yeast cell Anatomy 0.000 description 2
• 239000012138 yeast extract Substances 0.000 description 2
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
• DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
• WHTVZRBIWZFKQO-AWEZNQCLSA-N (S)-chloroquine Chemical compound ClC1=CC=C2C(N[C@@H](C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-AWEZNQCLSA-N 0.000 description 1
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
• NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
• DHEJDHPRYHYLKS-UHFFFAOYSA-N 2-(3,6-dihydroxy-9h-xanthen-9-yl)benzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C1C2=CC=C(O)C=C2OC2=CC(O)=CC=C21 DHEJDHPRYHYLKS-UHFFFAOYSA-N 0.000 description 1
• IVLXQGJVBGMLRR-UHFFFAOYSA-N 2-aminoacetic acid;hydron;chloride Chemical compound Cl.NCC(O)=O IVLXQGJVBGMLRR-UHFFFAOYSA-N 0.000 description 1
• TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
• 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 1
• 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
• 241001136792 Alle Species 0.000 description 1
• 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
• 101100082447 Arabidopsis thaliana PBL1 gene Proteins 0.000 description 1
• 206010003445 Ascites Diseases 0.000 description 1
• OAMLVOVXNKILLQ-BQBZGAKWSA-N Asp-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC(O)=O OAMLVOVXNKILLQ-BQBZGAKWSA-N 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 238000012935 Averaging Methods 0.000 description 1
• 102000019260 B-Cell Antigen Receptors Human genes 0.000 description 1
• 108010012919 B-Cell Antigen Receptors Proteins 0.000 description 1
• 241000304886 Bacilli Species 0.000 description 1
• 244000063299 Bacillus subtilis Species 0.000 description 1
• 235000014469 Bacillus subtilis Nutrition 0.000 description 1
• 102100021590 Bcl-2-like protein 10 Human genes 0.000 description 1
• 208000011691 Burkitt lymphomas Diseases 0.000 description 1
• 238000011740 C57BL/6 mouse Methods 0.000 description 1
• 101100291031 Caenorhabditis elegans gly-13 gene Proteins 0.000 description 1
• 101100533230 Caenorhabditis elegans ser-2 gene Proteins 0.000 description 1
• 101001007681 Candida albicans (strain WO-1) Kexin Proteins 0.000 description 1
• 241000282465 Canis Species 0.000 description 1
• 108010001857 Cell Surface Receptors Proteins 0.000 description 1
• 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
• 108010062580 Concanavalin A Proteins 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
• 102000004594 DNA Polymerase I Human genes 0.000 description 1
• 108010017826 DNA Polymerase I Proteins 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• 206010011878 Deafness Diseases 0.000 description 1
• 101710088194 Dehydrogenase Proteins 0.000 description 1
• 206010012438 Dermatitis atopic Diseases 0.000 description 1
• 229920002307 Dextran Polymers 0.000 description 1
• 239000004375 Dextrin Substances 0.000 description 1
• 229920001353 Dextrin Polymers 0.000 description 1
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
• 101100001677 Emericella variicolor andL gene Proteins 0.000 description 1
• 241000702371 Enterobacteria phage f1 Species 0.000 description 1
• 102100029727 Enteropeptidase Human genes 0.000 description 1
• 108010013369 Enteropeptidase Proteins 0.000 description 1
• 101710122227 Epstein-Barr nuclear antigen 1 Proteins 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 239000009484 FIBS Substances 0.000 description 1
• 241000282324 Felis Species 0.000 description 1
• 229920001917 Ficoll Polymers 0.000 description 1
• 208000004262 Food Hypersensitivity Diseases 0.000 description 1
• 206010016946 Food allergy Diseases 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• ZOXBSICWUDAOHX-GUBZILKMSA-N Glu-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CCC(O)=O ZOXBSICWUDAOHX-GUBZILKMSA-N 0.000 description 1
• 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 1
• 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 1
• SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
• 108010024636 Glutathione Proteins 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 101710088172 HTH-type transcriptional regulator RipA Proteins 0.000 description 1
• 208000032843 Hemorrhage Diseases 0.000 description 1
• 229920000209 Hexadimethrine bromide Polymers 0.000 description 1
• 102000005548 Hexokinase Human genes 0.000 description 1
• 108700040460 Hexokinases Proteins 0.000 description 1
• 241000231687 Heza Species 0.000 description 1
• 101000971082 Homo sapiens Bcl-2-like protein 10 Proteins 0.000 description 1
• 101001043807 Homo sapiens Interleukin-7 Proteins 0.000 description 1
• UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
• 108091029795 Intergenic region Proteins 0.000 description 1
• 102100026244 Interleukin-9 receptor Human genes 0.000 description 1
• 102000004195 Isomerases Human genes 0.000 description 1
• 108090000769 Isomerases Proteins 0.000 description 1
• 241000235649 Kluyveromyces Species 0.000 description 1
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
• 125000000415 L-cysteinyl group Chemical group O=C([*])[C@@](N([H])[H])([H])C([H])([H])S[H] 0.000 description 1
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
• 101800001509 Large capsid protein Proteins 0.000 description 1
• 101710192606 Latent membrane protein 2 Proteins 0.000 description 1
• 108090001090 Lectins Proteins 0.000 description 1
• 102000004856 Lectins Human genes 0.000 description 1
• GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
• VHNOAIFVYUQOOY-XUXIUFHCSA-N Lys-Arg-Ile Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VHNOAIFVYUQOOY-XUXIUFHCSA-N 0.000 description 1
• 240000000233 Melia azedarach Species 0.000 description 1
• 102000018697 Membrane Proteins Human genes 0.000 description 1
• 108010052285 Membrane Proteins Proteins 0.000 description 1
• 102000003792 Metallothionein Human genes 0.000 description 1
• 108090000157 Metallothionein Proteins 0.000 description 1
• 241000699666 Mus <mouse, genus> Species 0.000 description 1
• 241000699660 Mus musculus Species 0.000 description 1
• 101100438957 Mus musculus Cd8a gene Proteins 0.000 description 1
• 101001002703 Mus musculus Interleukin-4 Proteins 0.000 description 1
• NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
• 230000004988 N-glycosylation Effects 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 229930182555 Penicillin Natural products 0.000 description 1
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
• 108010087702 Penicillinase Proteins 0.000 description 1
• 102000001105 Phosphofructokinases Human genes 0.000 description 1
• 108010069341 Phosphofructokinases Proteins 0.000 description 1
• 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 1
• 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 1
• 108091000080 Phosphotransferase Proteins 0.000 description 1
• 241000235648 Pichia Species 0.000 description 1
• 241000288906 Primates Species 0.000 description 1
• CLJLVCYFABNTHP-DCAQKATOSA-N Pro-Leu-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O CLJLVCYFABNTHP-DCAQKATOSA-N 0.000 description 1
• OWQXAJQZLWHPBH-FXQIFTODSA-N Pro-Ser-Asn Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O OWQXAJQZLWHPBH-FXQIFTODSA-N 0.000 description 1
• 241000589516 Pseudomonas Species 0.000 description 1
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
• 102000013009 Pyruvate Kinase Human genes 0.000 description 1
• 108020005115 Pyruvate Kinase Proteins 0.000 description 1
• 239000012980 RPMI-1640 medium Substances 0.000 description 1
• 241000700159 Rattus Species 0.000 description 1
• 241000022563 Rema Species 0.000 description 1
• 239000012722 SDS sample buffer Substances 0.000 description 1
• 241000235070 Saccharomyces Species 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 101710173693 Short transient receptor potential channel 1 Proteins 0.000 description 1
• VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
• 108020004682 Single-Stranded DNA Proteins 0.000 description 1
• 238000002105 Southern blotting Methods 0.000 description 1
• 241000191940 Staphylococcus Species 0.000 description 1
• 229930006000 Sucrose Natural products 0.000 description 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
• 101710137500 T7 RNA polymerase Proteins 0.000 description 1
• 101710109576 Terminal protein Proteins 0.000 description 1
• 239000004098 Tetracycline Substances 0.000 description 1
• IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical group O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
• 235000011941 Tilia x europaea Nutrition 0.000 description 1
• 101710120037 Toxin CcdB Proteins 0.000 description 1
• LVTKHGUGBGNBPL-UHFFFAOYSA-N Trp-P-1 Chemical compound N1C2=CC=CC=C2C2=C1C(C)=C(N)N=C2C LVTKHGUGBGNBPL-UHFFFAOYSA-N 0.000 description 1
• 206010045240 Type I hypersensitivity Diseases 0.000 description 1
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
• 241000700605 Viruses Species 0.000 description 1
• DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
• MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000002411 adverse Effects 0.000 description 1
• 239000011543 agarose gel Substances 0.000 description 1
• VFRROHXSMXFLSN-SLPGGIOYSA-N aldehydo-D-glucose 6-phosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O VFRROHXSMXFLSN-SLPGGIOYSA-N 0.000 description 1
• 125000001931 aliphatic group Chemical group 0.000 description 1
• 150000001447 alkali salts Chemical class 0.000 description 1
• 230000004075 alteration Effects 0.000 description 1
• 125000003277 amino group Chemical group 0.000 description 1
• 229960003896 aminopterin Drugs 0.000 description 1
• 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
• 239000003957 anion exchange resin Substances 0.000 description 1
• 238000000137 annealing Methods 0.000 description 1
• 230000000890 antigenic effect Effects 0.000 description 1
• 239000003963 antioxidant agent Substances 0.000 description 1
• 235000006708 antioxidants Nutrition 0.000 description 1
• 239000008346 aqueous phase Substances 0.000 description 1
• 235000010323 ascorbic acid Nutrition 0.000 description 1
• 229960005070 ascorbic acid Drugs 0.000 description 1
• 239000011668 ascorbic acid Substances 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 201000008937 atopic dermatitis Diseases 0.000 description 1
• 230000001651 autotrophic effect Effects 0.000 description 1
• 238000012742 biochemical analysis Methods 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 230000000740 bleeding effect Effects 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 210000001124 body fluid Anatomy 0.000 description 1
• 239000010839 body fluid Substances 0.000 description 1
• 238000009835 boiling Methods 0.000 description 1
• 229940098773 bovine serum albumin Drugs 0.000 description 1
• 239000007975 buffered saline Substances 0.000 description 1
• 238000010804 cDNA synthesis Methods 0.000 description 1
• 239000001506 calcium phosphate Substances 0.000 description 1
• 229910000389 calcium phosphate Inorganic materials 0.000 description 1
• 235000011010 calcium phosphates Nutrition 0.000 description 1
• 229910052799 carbon Inorganic materials 0.000 description 1
• PFKFTWBEEFSNDU-UHFFFAOYSA-N carbonyldiimidazole Chemical compound C1=CN=CN1C(=O)N1C=CN=C1 PFKFTWBEEFSNDU-UHFFFAOYSA-N 0.000 description 1
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
• 150000001768 cations Chemical class 0.000 description 1
• 239000013592 cell lysate Substances 0.000 description 1
• 239000013553 cell monolayer Substances 0.000 description 1
• 210000002421 cell wall Anatomy 0.000 description 1
• 230000001413 cellular effect Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 229920002301 cellulose acetate Polymers 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 238000010382 chemical cross-linking Methods 0.000 description 1
• 229960003677 chloroquine Drugs 0.000 description 1
• WHTVZRBIWZFKQO-UHFFFAOYSA-N chloroquine Natural products ClC1=CC=C2C(NC(C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-UHFFFAOYSA-N 0.000 description 1
• 238000011210 chromatographic step Methods 0.000 description 1
• 238000004587 chromatography analysis Methods 0.000 description 1
• 239000013611 chromosomal DNA Substances 0.000 description 1
• 238000012411 cloning technique Methods 0.000 description 1
• 238000004590 computer program Methods 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 239000000356 contaminant Substances 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 239000003431 cross linking reagent Substances 0.000 description 1
• 230000001351 cycling effect Effects 0.000 description 1
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
• 102000003675 cytokine receptors Human genes 0.000 description 1
• 108010057085 cytokine receptors Proteins 0.000 description 1
• 230000001461 cytolytic effect Effects 0.000 description 1
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 1
• 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
• RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
• 229940009976 deoxycholate Drugs 0.000 description 1
• KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
• 230000008021 deposition Effects 0.000 description 1
• 238000001514 detection method Methods 0.000 description 1
• 229960002086 dextran Drugs 0.000 description 1
• 229960000633 dextran sulfate Drugs 0.000 description 1
• 235000019425 dextrin Nutrition 0.000 description 1
• 238000000502 dialysis Methods 0.000 description 1
• 238000010790 dilution Methods 0.000 description 1
• 239000012895 dilution Substances 0.000 description 1
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
• 231100000673 dose–response relationship Toxicity 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 230000003511 endothelial effect Effects 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 210000003743 erythrocyte Anatomy 0.000 description 1
• NTNZTEQNFHNYBC-UHFFFAOYSA-N ethyl 2-aminoacetate Chemical compound CCOC(=O)CN NTNZTEQNFHNYBC-UHFFFAOYSA-N 0.000 description 1
• DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 238000002270 exclusion chromatography Methods 0.000 description 1
• 239000011536 extraction buffer Substances 0.000 description 1
• 210000000416 exudates and transudate Anatomy 0.000 description 1
• 210000002950 fibroblast Anatomy 0.000 description 1
• 238000001914 filtration Methods 0.000 description 1
• 239000012847 fine chemical Substances 0.000 description 1
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
• 235000020932 food allergy Nutrition 0.000 description 1
• 230000005714 functional activity Effects 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 230000002496 gastric effect Effects 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 238000002523 gelfiltration Methods 0.000 description 1
• 238000010353 genetic engineering Methods 0.000 description 1
• 229960003180 glutathione Drugs 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• 239000007973 glycine-HCl buffer Substances 0.000 description 1
• 230000002414 glycolytic effect Effects 0.000 description 1
• 125000003147 glycosyl group Chemical group 0.000 description 1
• 210000003714 granulocyte Anatomy 0.000 description 1
• 230000003394 haemopoietic effect Effects 0.000 description 1
• 210000002443 helper t lymphocyte Anatomy 0.000 description 1
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
• 230000002607 hemopoietic effect Effects 0.000 description 1
• 229920000140 heteropolymer Polymers 0.000 description 1
• 210000003630 histaminocyte Anatomy 0.000 description 1
• 102000052622 human IL7 Human genes 0.000 description 1
• 210000005260 human cell Anatomy 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
• 230000002209 hydrophobic effect Effects 0.000 description 1
• 238000003018 immunoassay Methods 0.000 description 1
• 230000002621 immunoprecipitating effect Effects 0.000 description 1
• 238000001114 immunoprecipitation Methods 0.000 description 1
• 238000011065 in-situ storage Methods 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 208000030603 inherited susceptibility to asthma Diseases 0.000 description 1
• 238000002347 injection Methods 0.000 description 1
• 239000007924 injection Substances 0.000 description 1
• 230000002452 interceptive effect Effects 0.000 description 1
• 108040006862 interleukin-9 receptor activity proteins Proteins 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 238000010253 intravenous injection Methods 0.000 description 1
• 238000005342 ion exchange Methods 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 1
• 210000003292 kidney cell Anatomy 0.000 description 1
• 238000002372 labelling Methods 0.000 description 1
• 239000008101 lactose Substances 0.000 description 1
• 239000010410 layer Substances 0.000 description 1
• 239000002523 lectin Substances 0.000 description 1
• 230000003902 lesion Effects 0.000 description 1
• 210000000265 leukocyte Anatomy 0.000 description 1
• GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
• 108010052968 leupeptin Proteins 0.000 description 1
• 238000011694 lewis rat Methods 0.000 description 1
• 239000003446 ligand Substances 0.000 description 1
• 239000004571 lime Substances 0.000 description 1
• 150000002632 lipids Chemical class 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 210000004698 lymphocyte Anatomy 0.000 description 1
• 230000001589 lymphoproliferative effect Effects 0.000 description 1
• 239000006166 lysate Substances 0.000 description 1
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
• 230000002934 lysing effect Effects 0.000 description 1
• 238000013507 mapping Methods 0.000 description 1
• 210000003593 megakaryocyte Anatomy 0.000 description 1
• 108020004084 membrane receptors Proteins 0.000 description 1
• 229930182817 methionine Natural products 0.000 description 1
• 230000008747 mitogenic response Effects 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 230000004048 modification Effects 0.000 description 1
• 238000012986 modification Methods 0.000 description 1
• 230000009149 molecular binding Effects 0.000 description 1
• 239000003068 molecular probe Substances 0.000 description 1
• 238000012544 monitoring process Methods 0.000 description 1
• 238000009343 monoculture Methods 0.000 description 1
• 238000010172 mouse model Methods 0.000 description 1
• 210000004897 n-terminal region Anatomy 0.000 description 1
• 230000007935 neutral effect Effects 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 229910052757 nitrogen Inorganic materials 0.000 description 1
• 231100000252 nontoxic Toxicity 0.000 description 1
• 230000003000 nontoxic effect Effects 0.000 description 1
• 238000011580 nude mouse model Methods 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 230000003647 oxidation Effects 0.000 description 1
• 238000007254 oxidation reaction Methods 0.000 description 1
• 229940049954 penicillin Drugs 0.000 description 1
• 229950009506 penicillinase Drugs 0.000 description 1
• 229950000964 pepstatin Drugs 0.000 description 1
• 108010091212 pepstatin Proteins 0.000 description 1
• FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
• KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
• 210000005259 peripheral blood Anatomy 0.000 description 1
• 239000011886 peripheral blood Substances 0.000 description 1
• 210000003200 peritoneal cavity Anatomy 0.000 description 1
• 239000000546 pharmaceutical excipient Substances 0.000 description 1
• 239000012071 phase Substances 0.000 description 1
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 229910052698 phosphorus Inorganic materials 0.000 description 1
• 102000020233 phosphotransferase Human genes 0.000 description 1
• XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 description 1
• 230000003334 potential effect Effects 0.000 description 1
• 230000002028 premature Effects 0.000 description 1
• 238000002953 preparative HPLC Methods 0.000 description 1
• 238000003825 pressing Methods 0.000 description 1
• 238000012545 processing Methods 0.000 description 1
• 238000002731 protein assay Methods 0.000 description 1
• 238000001742 protein purification Methods 0.000 description 1
• 230000012743 protein tagging Effects 0.000 description 1
• 108010006230 protelin Proteins 0.000 description 1
• 230000017854 proteolysis Effects 0.000 description 1
• 230000002797 proteolythic effect Effects 0.000 description 1
• 230000002285 radioactive effect Effects 0.000 description 1
• 238000010814 radioimmunoprecipitation assay Methods 0.000 description 1
• 239000011541 reaction mixture Substances 0.000 description 1
• 230000009257 reactivity Effects 0.000 description 1
• 230000009467 reduction Effects 0.000 description 1
• 238000004153 renaturation Methods 0.000 description 1
• 230000003362 replicative effect Effects 0.000 description 1
• 238000011160 research Methods 0.000 description 1
• 239000011347 resin Substances 0.000 description 1
• 229920005989 resin Polymers 0.000 description 1
• 230000000284 resting effect Effects 0.000 description 1
• 108091008146 restriction endonucleases Proteins 0.000 description 1
• 206010039083 rhinitis Diseases 0.000 description 1
• 239000007320 rich medium Substances 0.000 description 1
• 238000005185 salting out Methods 0.000 description 1
• 239000012723 sample buffer Substances 0.000 description 1
• 238000013391 scatchard analysis Methods 0.000 description 1
• 239000013606 secretion vector Substances 0.000 description 1
• 102000029751 selenium binding Human genes 0.000 description 1
• 108091022876 selenium binding Proteins 0.000 description 1
• 239000000741 silica gel Substances 0.000 description 1
• 229910002027 silica gel Inorganic materials 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 1
• 229940001584 sodium metabisulfite Drugs 0.000 description 1
• 235000010262 sodium metabisulphite Nutrition 0.000 description 1
• RBWSWDPRDBEWCR-RKJRWTFHSA-N sodium;(2r)-2-[(2r)-3,4-dihydroxy-5-oxo-2h-furan-2-yl]-2-hydroxyethanolate Chemical compound [Na+].[O-]C[C@@H](O)[C@H]1OC(=O)C(O)=C1O RBWSWDPRDBEWCR-RKJRWTFHSA-N 0.000 description 1
• 239000007787 solid Substances 0.000 description 1
• 238000000527 sonication Methods 0.000 description 1
• 238000001228 spectrum Methods 0.000 description 1
• 230000003393 splenic effect Effects 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 238000010561 standard procedure Methods 0.000 description 1
• 238000011146 sterile filtration Methods 0.000 description 1
• 230000004936 stimulating effect Effects 0.000 description 1
• 239000011550 stock solution Substances 0.000 description 1
• 238000003860 storage Methods 0.000 description 1
• 229960005322 streptomycin Drugs 0.000 description 1
• 239000005720 sucrose Substances 0.000 description 1
• 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
• 229910052717 sulfur Inorganic materials 0.000 description 1
• 238000004114 suspension culture Methods 0.000 description 1
• 108700004027 tat Genes Proteins 0.000 description 1
• 101150098170 tat gene Proteins 0.000 description 1
• 229960002180 tetracycline Drugs 0.000 description 1
• 229930101283 tetracycline Natural products 0.000 description 1
• 235000019364 tetracycline Nutrition 0.000 description 1
• 150000003522 tetracyclines Chemical class 0.000 description 1
• 230000001225 therapeutic effect Effects 0.000 description 1
• 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 1
• 231100000331 toxic Toxicity 0.000 description 1
• 230000002588 toxic effect Effects 0.000 description 1
• 230000005026 transcription initiation Effects 0.000 description 1
• 230000005030 transcription termination Effects 0.000 description 1
• 238000003151 transfection method Methods 0.000 description 1
• 238000012546 transfer Methods 0.000 description 1
• 230000001131 transforming effect Effects 0.000 description 1
• 230000010474 transient expression Effects 0.000 description 1
• 230000014621 translational initiation Effects 0.000 description 1
• 230000032258 transport Effects 0.000 description 1
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
• 101150108727 trpl gene Proteins 0.000 description 1
• 238000000108 ultra-filtration Methods 0.000 description 1
• 241000701447 unidentified baculovirus Species 0.000 description 1
• 238000003260 vortexing Methods 0.000 description 1
• 238000005406 washing Methods 0.000 description 1
• 230000003442 weekly effect Effects 0.000 description 1
• 239000007222 ypd medium Substances 0.000 description 1