CA2016704C - Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche - Google Patents

Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche

Info

Publication number: CA2016704C
Authority: CA; Canada
Prior art keywords: gamma; group; mixture; ggt; nitrite
Prior art date: 1990-05-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA 2016704

Other languages

English (en)

Other versions

CA2016704A1 (fr

Inventor

Jane M. Rachel

Laura M. Smith

Linda R. Pfaltzgraff

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Osborn Group Inc

Original Assignee

Osborn Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-05-16

Filing date

1990-05-16

Publication date

1996-03-12

1990-05-16 Application filed by Osborn Laboratories Inc filed Critical Osborn Laboratories Inc

1990-05-16 Priority to CA 2016704 priority Critical patent/CA2016704C/fr

1991-11-16 Publication of CA2016704A1 publication Critical patent/CA2016704A1/fr

1996-03-12 Application granted granted Critical

1996-03-12 Publication of CA2016704C publication Critical patent/CA2016704C/fr

2010-05-15 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

210000004369 blood Anatomy 0.000 title claims abstract description 85
239000008280 blood Substances 0.000 title claims abstract description 85
108020004206 Gamma-glutamyltransferase Proteins 0.000 title claims abstract description 62
102000006640 gamma-Glutamyltransferase Human genes 0.000 title claims abstract description 62
238000003556 assay Methods 0.000 title description 10
239000007788 liquid Substances 0.000 title description 2
238000000034 method Methods 0.000 claims abstract description 35
230000000694 effects Effects 0.000 claims abstract description 30
239000012530 fluid Substances 0.000 claims abstract description 29
239000000203 mixture Substances 0.000 claims abstract description 25
239000000758 substrate Substances 0.000 claims abstract description 9
238000006149 azo coupling reaction Methods 0.000 claims abstract description 8
239000003599 detergent Substances 0.000 claims abstract description 4
239000002250 absorbent Substances 0.000 claims description 17
230000002745 absorbent Effects 0.000 claims description 17
IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 14
LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 12
230000003381 solubilizing effect Effects 0.000 claims description 10
102000004190 Enzymes Human genes 0.000 claims description 9
108090000790 Enzymes Proteins 0.000 claims description 9
MZNYWPRCVDMOJG-UHFFFAOYSA-N N-(1-naphthyl)ethylenediamine dihydrochloride Chemical compound [Cl-].[Cl-].C1=CC=C2C([NH2+]CC[NH3+])=CC=CC2=C1 MZNYWPRCVDMOJG-UHFFFAOYSA-N 0.000 claims description 8
239000011521 glass Substances 0.000 claims description 7
239000002253 acid Substances 0.000 claims description 6
235000011167 hydrochloric acid Nutrition 0.000 claims description 6
235000010288 sodium nitrite Nutrition 0.000 claims description 6
GEHMBYLTCISYNY-UHFFFAOYSA-N Ammonium sulfamate Chemical compound [NH4+].NS([O-])(=O)=O GEHMBYLTCISYNY-UHFFFAOYSA-N 0.000 claims description 5
235000011054 acetic acid Nutrition 0.000 claims description 5
125000002642 gamma-glutamyl group Chemical group 0.000 claims description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
AZFNGPAYDKGCRB-XCPIVNJJSA-M [(1s,2s)-2-amino-1,2-diphenylethyl]-(4-methylphenyl)sulfonylazanide;chlororuthenium(1+);1-methyl-4-propan-2-ylbenzene Chemical compound [Ru+]Cl.CC(C)C1=CC=C(C)C=C1.C1=CC(C)=CC=C1S(=O)(=O)[N-][C@@H](C=1C=CC=CC=1)[C@@H](N)C1=CC=CC=C1 AZFNGPAYDKGCRB-XCPIVNJJSA-M 0.000 claims description 4
239000007864 aqueous solution Substances 0.000 claims description 4
238000001035 drying Methods 0.000 claims description 4
LNOPIUAQISRISI-UHFFFAOYSA-N n'-hydroxy-2-propan-2-ylsulfonylethanimidamide Chemical compound CC(C)S(=O)(=O)CC(N)=NO LNOPIUAQISRISI-UHFFFAOYSA-N 0.000 claims description 4
235000010289 potassium nitrite Nutrition 0.000 claims description 4
239000004304 potassium nitrite Substances 0.000 claims description 4
229920003043 Cellulose fiber Polymers 0.000 claims description 3
229920000742 Cotton Polymers 0.000 claims description 3
239000005909 Kieselgur Substances 0.000 claims description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
150000001243 acetic acids Chemical class 0.000 claims description 3
235000015165 citric acid Nutrition 0.000 claims description 3
238000010790 dilution Methods 0.000 claims description 3
239000012895 dilution Substances 0.000 claims description 3
235000019253 formic acid Nutrition 0.000 claims description 3
230000002949 hemolytic effect Effects 0.000 claims description 3
235000011007 phosphoric acid Nutrition 0.000 claims description 3
235000011044 succinic acid Nutrition 0.000 claims description 3
235000010338 boric acid Nutrition 0.000 claims description 2
239000011780 sodium chloride Substances 0.000 claims description 2
RUFPHBVGCFYCNW-UHFFFAOYSA-N 1-naphthylamine Chemical compound C1=CC=C2C(N)=CC=CC2=C1 RUFPHBVGCFYCNW-UHFFFAOYSA-N 0.000 claims 4
JBIJLHTVPXGSAM-UHFFFAOYSA-N 2-naphthylamine Chemical compound C1=CC=CC2=CC(N)=CC=C21 JBIJLHTVPXGSAM-UHFFFAOYSA-N 0.000 claims 2
ZBIBQNVRTVLOHQ-UHFFFAOYSA-N 5-aminonaphthalen-1-ol Chemical compound C1=CC=C2C(N)=CC=CC2=C1O ZBIBQNVRTVLOHQ-UHFFFAOYSA-N 0.000 claims 2
QYFYIOWLBSPSDM-UHFFFAOYSA-N 6-aminonaphthalen-1-ol Chemical compound OC1=CC=CC2=CC(N)=CC=C21 QYFYIOWLBSPSDM-UHFFFAOYSA-N 0.000 claims 2
HMNPDEGBVWDHAR-UHFFFAOYSA-N 8-aminonaphthalen-1-ol Chemical compound C1=CC(O)=C2C(N)=CC=CC2=C1 HMNPDEGBVWDHAR-UHFFFAOYSA-N 0.000 claims 2
239000007975 buffered saline Substances 0.000 claims 1
239000000123 paper Substances 0.000 claims 1
238000006243 chemical reaction Methods 0.000 abstract description 13
239000007795 chemical reaction product Substances 0.000 abstract description 9
238000006911 enzymatic reaction Methods 0.000 abstract description 9
239000000243 solution Substances 0.000 abstract description 8
238000004458 analytical method Methods 0.000 abstract description 7
238000005119 centrifugation Methods 0.000 abstract description 2
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 abstract description 2
239000002953 phosphate buffered saline Substances 0.000 abstract description 2
230000006920 protein precipitation Effects 0.000 abstract 1
239000000523 sample Substances 0.000 description 47
YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 14
TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 9
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
210000002966 serum Anatomy 0.000 description 8
108010008488 Glycylglycine Proteins 0.000 description 7
229940043257 glycylglycine Drugs 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
239000007983 Tris buffer Substances 0.000 description 6
239000011541 reaction mixture Substances 0.000 description 6
102000001554 Hemoglobins Human genes 0.000 description 5
108010054147 Hemoglobins Proteins 0.000 description 5
238000010521 absorption reaction Methods 0.000 description 5
150000008049 diazo compounds Chemical class 0.000 description 5
238000012360 testing method Methods 0.000 description 5
230000006378 damage Effects 0.000 description 4
125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 4
201000010099 disease Diseases 0.000 description 4
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
WZVXLJYENHHFPD-UHFFFAOYSA-N methylaminomethanol;hydrochloride Chemical compound Cl.CNCO WZVXLJYENHHFPD-UHFFFAOYSA-N 0.000 description 4
239000012047 saturated solution Substances 0.000 description 4
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
206010018910 Haemolysis Diseases 0.000 description 3
238000013019 agitation Methods 0.000 description 3
230000015572 biosynthetic process Effects 0.000 description 3
239000007853 buffer solution Substances 0.000 description 3
230000008588 hemolysis Effects 0.000 description 3
210000004185 liver Anatomy 0.000 description 3
238000000926 separation method Methods 0.000 description 3
238000005063 solubilization Methods 0.000 description 3
230000007928 solubilization Effects 0.000 description 3
102000004506 Blood Proteins Human genes 0.000 description 2
108010017384 Blood Proteins Proteins 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
238000004925 denaturation Methods 0.000 description 2
230000036425 denaturation Effects 0.000 description 2
238000011161 development Methods 0.000 description 2
210000003743 erythrocyte Anatomy 0.000 description 2
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
208000019423 liver disease Diseases 0.000 description 2
NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 2
210000000496 pancreas Anatomy 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
230000035484 reaction time Effects 0.000 description 2
JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
101001003194 Eleusine coracana Alpha-amylase/trypsin inhibitor Proteins 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
108010024636 Glutathione Proteins 0.000 description 1
206010033645 Pancreatitis Diseases 0.000 description 1
229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
229920001214 Polysorbate 60 Polymers 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
YVPYQUNUQOZFHG-UHFFFAOYSA-N amidotrizoic acid Chemical compound CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C(O)=O)=C1I YVPYQUNUQOZFHG-UHFFFAOYSA-N 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
239000011260 aqueous acid Substances 0.000 description 1
210000000013 bile duct Anatomy 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
125000005619 boric acid group Chemical group 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
229960003180 glutathione Drugs 0.000 description 1
231100000206 health hazard Toxicity 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
238000003367 kinetic assay Methods 0.000 description 1
230000031700 light absorption Effects 0.000 description 1
230000005976 liver dysfunction Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
208000010125 myocardial infarction Diseases 0.000 description 1
208000018389 neoplasm of cerebral hemisphere Diseases 0.000 description 1
230000007170 pathology Effects 0.000 description 1
-1 polyoxyethylene Polymers 0.000 description 1
229920000136 polysorbate Polymers 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
210000002307 prostate Anatomy 0.000 description 1
238000011002 quantification Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
239000011369 resultant mixture Substances 0.000 description 1
238000005070 sampling Methods 0.000 description 1
238000002791 soaking Methods 0.000 description 1
238000002798 spectrophotometry method Methods 0.000 description 1
150000003444 succinic acids Chemical class 0.000 description 1
IIACRCGMVDHOTQ-UHFFFAOYSA-M sulfamate Chemical compound NS([O-])(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-M 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
238000012546 transfer Methods 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
210000002700 urine Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91074—Aminoacyltransferases (general) (2.3.2)

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA 2016704 1990-05-16 1990-05-16 Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche Expired - Fee Related CA2016704C (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA 2016704 CA2016704C (fr)	1990-05-16	1990-05-16	Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA 2016704 CA2016704C (fr)	1990-05-16	1990-05-16	Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche

Publications (2)

Publication Number	Publication Date
CA2016704A1 CA2016704A1 (fr)	1991-11-16
CA2016704C true CA2016704C (fr)	1996-03-12

Family

ID=4144978

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2016704 Expired - Fee Related CA2016704C (fr)	1990-05-16	1990-05-16	Appareil et methode pour le dosage de la gamma-glutamyltransferase dans le sang entier liquide et seche

Country Status (1)

Country	Link
CA (1)	CA2016704C (fr)

1990
- 1990-05-16 CA CA 2016704 patent/CA2016704C/fr not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CA2016704A1 (fr)	1991-11-16

Legal Events

Date	Code	Title	Description
1992-03-18	EEER	Examination request
2003-05-15	MKLA	Lapsed

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