CA2045614C - Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe - Google Patents
Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexeInfo
- Publication number
- CA2045614C CA2045614C CA002045614A CA2045614A CA2045614C CA 2045614 C CA2045614 C CA 2045614C CA 002045614 A CA002045614 A CA 002045614A CA 2045614 A CA2045614 A CA 2045614A CA 2045614 C CA2045614 C CA 2045614C
- Authority
- CA
- Canada
- Prior art keywords
- cell
- proliferation
- determining
- optically
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000035755 proliferation Effects 0.000 title claims abstract description 110
- 238000000034 method Methods 0.000 title claims description 25
- 210000004027 cell Anatomy 0.000 claims abstract description 130
- 210000003855 cell nucleus Anatomy 0.000 claims abstract description 78
- 239000000126 substance Substances 0.000 claims abstract description 77
- 230000003287 optical effect Effects 0.000 claims abstract description 41
- 238000010186 staining Methods 0.000 claims abstract description 6
- 230000005540 biological transmission Effects 0.000 claims description 11
- 230000002708 enhancing effect Effects 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 230000002055 immunohistochemical effect Effects 0.000 claims 1
- 238000003672 processing method Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 18
- 238000002360 preparation method Methods 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 13
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 7
- 230000010076 replication Effects 0.000 description 7
- 238000003491 array Methods 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 5
- 102000003992 Peroxidases Human genes 0.000 description 5
- GPKUGWDQUVWHIC-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine tetrahydrochloride Chemical compound Cl.Cl.Cl.Cl.NNC1=CC=C(C=C1)C1=CC=C(NN)C=C1 GPKUGWDQUVWHIC-UHFFFAOYSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 108050006400 Cyclin Proteins 0.000 description 4
- 102000016736 Cyclin Human genes 0.000 description 4
- 230000018199 S phase Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 230000003211 malignant effect Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- IQFYYKKMVGJFEH-OFKYTIFKSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(tritiooxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO[3H])O[C@H]1N1C(=O)NC(=O)C(C)=C1 IQFYYKKMVGJFEH-OFKYTIFKSA-N 0.000 description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 230000032823 cell division Effects 0.000 description 3
- 238000004891 communication Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- DWCZIOOZPIDHAB-UHFFFAOYSA-L methyl green Chemical compound [Cl-].[Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)[N+](C)(C)C)=C1C=CC(=[N+](C)C)C=C1 DWCZIOOZPIDHAB-UHFFFAOYSA-L 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 210000001082 somatic cell Anatomy 0.000 description 3
- 230000035519 G0 Phase Effects 0.000 description 2
- 230000010337 G2 phase Effects 0.000 description 2
- 208000002151 Pleural effusion Diseases 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 2
- 208000031513 cyst Diseases 0.000 description 2
- IDAQSADEMXDTKN-UHFFFAOYSA-L ethyl green Chemical compound [Cl-].[Br-].C1=CC([N+](C)(C)CC)=CC=C1C(C=1C=CC(=CC=1)N(C)C)=C1C=CC(=[N+](C)C)C=C1 IDAQSADEMXDTKN-UHFFFAOYSA-L 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000010562 histological examination Methods 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 230000016507 interphase Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- KJDSORYAHBAGPP-UHFFFAOYSA-N 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydron;tetrachloride Chemical compound Cl.Cl.Cl.Cl.C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 KJDSORYAHBAGPP-UHFFFAOYSA-N 0.000 description 1
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
- WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101000929583 Homo sapiens N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 230000027311 M phase Effects 0.000 description 1
- 102100036658 N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Human genes 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 208000006994 Precancerous Conditions Diseases 0.000 description 1
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010256 biochemical assay Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229950004398 broxuridine Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- -1 cerebrospinal fluid Substances 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 230000035773 mitosis phase Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1765—Method using an image detector and processing of image signal
- G01N2021/177—Detector of the video camera type
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Méthode et appareil de traitement des images (10) donnant un indice de prolifération d'un échantillon de cellules par coloration des cellules à l'aide d'un chromogène comme substance indicative de la prolifération et par contre-coloration pour le dénombrement des noyaux des cellules. Le chromogène est activé par un conjugué anticorps-enzyme qui se lie à la substance de prolifération pour produire un échantillon de cellules colorées. L'échantillon de cellules colorées est observé à l'aide d'un microscope optique (12), faisant partie intégrante de l'appareil, qui donne une image agrandie de l'échantillon de cellules. L'appareil filtre optiquement (18) l'image de l'échantillon et produit une paire d'images optiquement améliorées de la substance de prolifération et du noyau des cellules. Les images améliorées sont analysées électroniquement pour déterminer le nombre de noyaux de cellules et la quantité de substance de prolifération apparaissant dans les images respectivement. Les résultats sont alors comparés pour créer un indice de prolifération pour la portion de l'échantillon de cellules apparaissant dans l'image de l'échantillon.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US315,289 | 1989-02-24 | ||
| US07/315,289 US5086476A (en) | 1985-11-04 | 1989-02-24 | Method and apparatus for determining a proliferation index of a cell sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2045614A1 CA2045614A1 (fr) | 1990-08-25 |
| CA2045614C true CA2045614C (fr) | 1997-09-30 |
Family
ID=23223738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002045614A Expired - Fee Related CA2045614C (fr) | 1989-02-24 | 1990-02-23 | Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0460071A4 (fr) |
| CA (1) | CA2045614C (fr) |
| WO (1) | WO1990010277A1 (fr) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5889881A (en) * | 1992-10-14 | 1999-03-30 | Oncometrics Imaging Corp. | Method and apparatus for automatically detecting malignancy-associated changes |
| US6026174A (en) * | 1992-10-14 | 2000-02-15 | Accumed International, Inc. | System and method for automatically detecting malignant cells and cells having malignancy-associated changes |
| BRPI0507169A (pt) | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos |
| US7632924B2 (en) | 2004-06-18 | 2009-12-15 | Ambrx, Inc. | Antigen-binding polypeptides and their uses |
| WO2006091231A2 (fr) | 2004-07-21 | 2006-08-31 | Ambrx, Inc. | Polypeptides biosynthetiques obtenus a partir d'acides amines codes par voie non naturelle |
| BRPI0519430A2 (pt) | 2004-12-22 | 2009-02-10 | Ambrx Inc | hormânio do crescimento humano modificado |
| EP1836316A4 (fr) | 2004-12-22 | 2009-07-22 | Ambrx Inc | Procédés pour l'expression et la purification d'hormone de croissance humaine recombinante |
| CN103520735B (zh) | 2004-12-22 | 2015-11-25 | Ambrx公司 | 包含非天然编码的氨基酸的人生长激素配方 |
| EP2399893B1 (fr) | 2004-12-22 | 2018-08-15 | Ambrx, Inc. | Compositions contenant des acides aminés et des polypeptides non naturels, procédés les impliquant et leurs utilisations |
| EP1836298B1 (fr) | 2004-12-22 | 2012-01-18 | Ambrx, Inc. | Compositions de synthetase d'aminoacyl-arnt et utilisations correspondantes |
| AU2006255122B2 (en) | 2005-06-03 | 2010-10-21 | Ambrx, Inc. | Improved human interferon molecules and their uses |
| AU2005335491B2 (en) | 2005-08-18 | 2010-11-25 | Ambrx, Inc. | Compositions of tRNA and uses thereof |
| DK2339014T3 (en) | 2005-11-16 | 2015-07-20 | Ambrx Inc | Methods and compositions comprising non-natural amino acids |
| JP5399906B2 (ja) | 2006-09-08 | 2014-01-29 | アンブルックス，インコーポレイテッド | 脊椎動物細胞用のハイブリッドサプレッサーｔｒｎａ |
| EP2064333B1 (fr) | 2006-09-08 | 2014-02-26 | Ambrx, Inc. | Transcription d'arnt suppresseur dans des cellules de vertébrés |
| CN106008699A (zh) | 2006-09-08 | 2016-10-12 | Ambrx公司 | 经修饰的人类血浆多肽或Fc骨架和其用途 |
| CN107501407B (zh) | 2007-03-30 | 2022-03-18 | Ambrx公司 | 经修饰fgf-21多肽和其用途 |
| US8114630B2 (en) | 2007-05-02 | 2012-02-14 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
| MX2010005317A (es) | 2007-11-20 | 2010-06-02 | Ambrx Inc | Polipeptidos de insulina modificados y sus usos. |
| MX344166B (es) | 2008-02-08 | 2016-12-07 | Ambrx Inc | Leptina-polipeptidos modificados y sus usos. |
| CN102159230A (zh) | 2008-07-23 | 2011-08-17 | Ambrx公司 | 经修饰的牛g-csf多肽和其用途 |
| BR122012024318A2 (pt) | 2008-09-26 | 2019-07-30 | Ambrx, Inc. | Polipeptídeos modificados de eritropoetina animal e seus usos |
| AU2009296267B2 (en) | 2008-09-26 | 2013-10-31 | Ambrx, Inc. | Non-natural amino acid replication-dependent microorganisms and vaccines |
| BR112012015597A2 (pt) | 2009-12-21 | 2017-01-31 | Ambrx Inc | peptídeos de somatotropina suínos modificados e seus usos |
| MX349301B (es) | 2009-12-21 | 2017-07-21 | Ambrx Inc | Polipéptidos de somatotropina bovina modificados y sus usos. |
| US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
| EA030886B1 (ru) | 2010-08-17 | 2018-10-31 | Амбркс, Инк. | Модифицированные полипептиды релаксина, содержащие некодируемую в природе аминокислоту, связанную с полимером, и их применение |
| TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
| JP6355082B2 (ja) | 2013-07-18 | 2018-07-11 | パナソニックＩｐマネジメント株式会社 | 病理診断支援装置及び病理診断支援方法 |
| CN114805532A (zh) | 2014-10-24 | 2022-07-29 | 百时美施贵宝公司 | 修饰的fgf-21多肽及其用途 |
| PE20191716A1 (es) | 2017-02-08 | 2019-12-05 | Bristol Myers Squibb Co | Polipeptidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos |
| CN111413504B (zh) * | 2020-04-03 | 2022-01-28 | 河北医科大学第四医院 | 一种辅助判读ki67增殖指数的标准比对卡 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4125828A (en) * | 1972-08-04 | 1978-11-14 | Med-El Inc. | Method and apparatus for automated classification and analysis of cells |
| US3999047A (en) * | 1972-09-05 | 1976-12-21 | Green James E | Method and apparatus utilizing color algebra for analyzing scene regions |
| US4199748A (en) * | 1976-11-01 | 1980-04-22 | Rush-Presbyterian-St. Luke's Medical Center | Automated method and apparatus for classification of cells with application to the diagnosis of anemia |
| JPS5661650A (en) * | 1979-10-24 | 1981-05-27 | Omron Tateisi Electronics Co | Analyzing device of cell |
| US4741043B1 (en) * | 1985-11-04 | 1994-08-09 | Cell Analysis Systems Inc | Method of and apparatus for image analyses of biological specimens |
-
1990
- 1990-02-23 CA CA002045614A patent/CA2045614C/fr not_active Expired - Fee Related
- 1990-02-23 WO PCT/US1990/000999 patent/WO1990010277A1/fr not_active Ceased
- 1990-02-23 EP EP19900904138 patent/EP0460071A4/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2045614A1 (fr) | 1990-08-25 |
| EP0460071A4 (en) | 1993-02-03 |
| WO1990010277A1 (fr) | 1990-09-07 |
| EP0460071A1 (fr) | 1991-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2045614C (fr) | Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe | |
| US5086476A (en) | Method and apparatus for determining a proliferation index of a cell sample | |
| US5252487A (en) | Method and apparatus for determining the amount of oncogene protein product in a cell sample | |
| US5008185A (en) | Methods and apparatus for the quantitation of nuclear proteins | |
| US6656683B1 (en) | Laser scanning cytology with digital image capture | |
| Kaleem et al. | Flow cytometric analysis of acute leukemias | |
| US5473706A (en) | Method and apparatus for automated assay of biological specimens | |
| EP1131631B1 (fr) | Methode d'analyse de cellules in situ | |
| US5202931A (en) | Methods and apparatus for the quantitation of nuclear protein | |
| US5202230A (en) | Methods of detecting cut cells in a tissue section | |
| US9607372B2 (en) | Automated bone marrow cellularity determination | |
| EP1065496A2 (fr) | Procédé et dispositif pour l'extraction d'images séparées à partir d'une pluralité de chromogènes dans un échantillon biologique | |
| Parry et al. | Cancer detection by quantitative fluorescence image analysis | |
| Ornberg et al. | Analysis of stained objects in histological sections by spectral imaging and differential absorption | |
| JP2002296275A (ja) | 少なくとも２種の異なる分子マーカーを同時に測定することによって腫瘍およびそれらの前駆体段階を検出する場合に臨床的特異性を増強する方法 | |
| Qiao et al. | A case-cohort study of an early biomarker of lung cancer in a screening cohort of Yunnan tin miners in China. | |
| US20150160197A1 (en) | System and Method for Analyzing Samples Labeled with 5, 10, 15, 20 Tetrakis (4-Carboxyphenyl) Porphine (TCPP) | |
| JP2002521682A5 (fr) | ||
| Satturwar et al. | Ki‐67 proliferation index in neuroendocrine tumors: Can augmented reality microscopy with image analysis improve scoring? | |
| Zhou et al. | A multiple wavelength algorithm in color image analysis and its applications in stain decomposition in microscopy images | |
| Clatch et al. | Multiparameter analysis of DNA content and cytokeratin expression in breast carcinoma by laser scanning cytometry | |
| Hemstreet III et al. | Quantitative fluorescence image analysis in bladder cancer screening | |
| JPH05500152A (ja) | 細胞サンプルの増殖指数を測定するための方法及び装置 | |
| WO2023239998A1 (fr) | Procédé de génération d'annotations d'image virtuellement colorées basées sur une inférence | |
| Clatch | Immunophenotyping of hematological malignancies by laser scanning cytometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |