CA2047752C - Hormone response element compositions and assay - Google Patents

Hormone response element compositions and assay Download PDF

Info

Publication number: CA2047752C
Authority: CA; Canada
Prior art keywords: receptor; response element; ligand; orphan receptor; hormone
Prior art date: 1989-03-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA002047752A

Other languages

English (en)

French (fr)

Other versions

CA2047752A1 (en

Inventor

Ronald M. Evans

Kazuhiko Umesono

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Salk Institute for Biological Studies

Original Assignee

Salk Institute for Biological Studies

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-03-17

Filing date

1990-03-16

Publication date

2001-07-10

1990-03-16 Application filed by Salk Institute for Biological Studies filed Critical Salk Institute for Biological Studies

1990-09-18 Publication of CA2047752A1 publication Critical patent/CA2047752A1/en

2001-07-10 Application granted granted Critical

2001-07-10 Publication of CA2047752C publication Critical patent/CA2047752C/en

2010-03-16 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

108091027981 Response element Proteins 0.000 title claims description 54
229940088597 hormone Drugs 0.000 title claims description 42
239000005556 hormone Substances 0.000 title claims description 42
238000003556 assay Methods 0.000 title abstract description 26
239000000203 mixture Substances 0.000 title abstract description 10
102000005962 receptors Human genes 0.000 claims abstract description 93
108020003175 receptors Proteins 0.000 claims abstract description 93
230000004568 DNA-binding Effects 0.000 claims abstract description 60
239000003446 ligand Substances 0.000 claims abstract description 45
108090000623 proteins and genes Proteins 0.000 claims abstract description 37
238000000034 method Methods 0.000 claims abstract description 32
235000001014 amino acid Nutrition 0.000 claims description 58
150000001413 amino acids Chemical class 0.000 claims description 57
108090000079 Glucocorticoid Receptors Proteins 0.000 claims description 41
102000016978 Orphan receptors Human genes 0.000 claims description 29
108070000031 Orphan receptors Proteins 0.000 claims description 29
108700008625 Reporter Genes Proteins 0.000 claims description 27
102000015694 estrogen receptors Human genes 0.000 claims description 24
108010038795 estrogen receptors Proteins 0.000 claims description 24
108091028043 Nucleic acid sequence Proteins 0.000 claims description 22
230000006698 induction Effects 0.000 claims description 21
229940011871 estrogen Drugs 0.000 claims description 14
239000000262 estrogen Substances 0.000 claims description 14
108020004414 DNA Proteins 0.000 claims description 13
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 13
239000003862 glucocorticoid Substances 0.000 claims description 13
230000002950 deficient Effects 0.000 claims description 12
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 8
108020001756 ligand binding domains Proteins 0.000 claims description 8
239000004471 Glycine Substances 0.000 claims description 7
101100069696 Arabidopsis thaliana GTG2 gene Proteins 0.000 claims description 6
230000009977 dual effect Effects 0.000 claims description 6
101100069695 Arabidopsis thaliana GTG1 gene Proteins 0.000 claims description 5
235000013922 glutamic acid Nutrition 0.000 claims description 5
239000004220 glutamic acid Substances 0.000 claims description 5
238000002741 site-directed mutagenesis Methods 0.000 claims description 5
108090000331 Firefly luciferases Proteins 0.000 claims description 4
230000035772 mutation Effects 0.000 claims description 4
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 claims description 3
125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims description 3
150000007523 nucleic acids Chemical group 0.000 claims description 3
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims description 2
125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims description 2
238000012544 monitoring process Methods 0.000 claims 5
150000008574 D-amino acids Chemical class 0.000 claims 1
102100033417 Glucocorticoid receptor Human genes 0.000 claims 1
102000004217 thyroid hormone receptors Human genes 0.000 abstract description 16
108090000721 thyroid hormone receptors Proteins 0.000 abstract description 16
102000005969 steroid hormone receptors Human genes 0.000 abstract description 10
102000004169 proteins and genes Human genes 0.000 abstract description 9
150000003431 steroids Chemical class 0.000 abstract description 9
108091008039 hormone receptors Proteins 0.000 abstract description 8
108020003113 steroid hormone receptors Proteins 0.000 abstract description 7
229940024606 amino acid Drugs 0.000 description 55
102000003676 Glucocorticoid Receptors Human genes 0.000 description 40
239000013612 plasmid Substances 0.000 description 29
101000926939 Homo sapiens Glucocorticoid receptor Proteins 0.000 description 28
210000004027 cell Anatomy 0.000 description 28
230000000694 effects Effects 0.000 description 22
239000002773 nucleotide Substances 0.000 description 19
125000003729 nucleotide group Chemical group 0.000 description 19
108060001084 Luciferase Proteins 0.000 description 15
239000005089 Luciferase Substances 0.000 description 14
239000011701 zinc Substances 0.000 description 12
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 11
235000018417 cysteine Nutrition 0.000 description 11
229910052725 zinc Inorganic materials 0.000 description 11
150000001945 cysteines Chemical class 0.000 description 10
230000006870 function Effects 0.000 description 10
230000004043 responsiveness Effects 0.000 description 10
125000003275 alpha amino acid group Chemical group 0.000 description 9
230000001419 dependent effect Effects 0.000 description 9
230000008859 change Effects 0.000 description 8
230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 8
235000018102 proteins Nutrition 0.000 description 8
101001112293 Homo sapiens Retinoic acid receptor alpha Proteins 0.000 description 7
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 7
239000002299 complementary DNA Substances 0.000 description 7
239000012634 fragment Substances 0.000 description 7
239000013613 expression plasmid Substances 0.000 description 6
101150007028 HTRA1 gene Proteins 0.000 description 5
AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 5
PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 5
238000010353 genetic engineering Methods 0.000 description 5
238000006467 substitution reaction Methods 0.000 description 5
239000005495 thyroid hormone Substances 0.000 description 5
229940036555 thyroid hormone Drugs 0.000 description 5
238000013518 transcription Methods 0.000 description 5
230000035897 transcription Effects 0.000 description 5
101100460844 Mus musculus Nr2f6 gene Proteins 0.000 description 4
108091034117 Oligonucleotide Proteins 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 4
229960003957 dexamethasone Drugs 0.000 description 4
229960002989 glutamic acid Drugs 0.000 description 4
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
230000004044 response Effects 0.000 description 4
108090000064 retinoic acid receptors Proteins 0.000 description 4
102000003702 retinoic acid receptors Human genes 0.000 description 4
239000003270 steroid hormone Substances 0.000 description 4
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 3
108020004635 Complementary DNA Proteins 0.000 description 3
108010032363 ERRalpha estrogen-related receptor Proteins 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
101000851696 Homo sapiens Steroid hormone receptor ERR2 Proteins 0.000 description 3
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 3
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
108091008650 NR0A2 Proteins 0.000 description 3
108010085012 Steroid Receptors Proteins 0.000 description 3
102100036832 Steroid hormone receptor ERR1 Human genes 0.000 description 3
102100036831 Steroid hormone receptor ERR2 Human genes 0.000 description 3
239000002253 acid Substances 0.000 description 3
230000004913 activation Effects 0.000 description 3
239000000427 antigen Substances 0.000 description 3
108091007433 antigens Proteins 0.000 description 3
102000036639 antigens Human genes 0.000 description 3
230000027455 binding Effects 0.000 description 3
230000033228 biological regulation Effects 0.000 description 3
108700010039 chimeric receptor Proteins 0.000 description 3
238000012217 deletion Methods 0.000 description 3
230000037430 deletion Effects 0.000 description 3
108020004067 estrogen-related receptors Proteins 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
238000000338 in vitro Methods 0.000 description 3
238000001890 transfection Methods 0.000 description 3
QIVBCDIJIAJPQS-UHFFFAOYSA-N tryptophan Chemical compound C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
241000282693 Cercopithecidae Species 0.000 description 2
108091026890 Coding region Proteins 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
102000003979 Mineralocorticoid Receptors Human genes 0.000 description 2
108090000375 Mineralocorticoid Receptors Proteins 0.000 description 2
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
101001109695 Rattus norvegicus Nuclear receptor subfamily 4 group A member 1 Proteins 0.000 description 2
238000012512 characterization method Methods 0.000 description 2
239000013522 chelant Substances 0.000 description 2
238000006471 dimerization reaction Methods 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
229960005309 estradiol Drugs 0.000 description 2
239000003630 growth substance Substances 0.000 description 2
230000003054 hormonal effect Effects 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
230000003993 interaction Effects 0.000 description 2
210000003292 kidney cell Anatomy 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000002703 mutagenesis Methods 0.000 description 2
231100000350 mutagenesis Toxicity 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
230000000638 stimulation Effects 0.000 description 2
230000001629 suppression Effects 0.000 description 2
210000001685 thyroid gland Anatomy 0.000 description 2
102000009310 vitamin D receptors Human genes 0.000 description 2
108050000156 vitamin D receptors Proteins 0.000 description 2
108010077544 Chromatin Proteins 0.000 description 1
CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
241001131785 Escherichia coli HB101 Species 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
101100218662 Felis catus GLB1 gene Proteins 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
PFMUCCYYAAFKTH-YFKPBYRVSA-N Gly-Met Chemical compound CSCC[C@@H](C(O)=O)NC(=O)CN PFMUCCYYAAFKTH-YFKPBYRVSA-N 0.000 description 1
101000882584 Homo sapiens Estrogen receptor Proteins 0.000 description 1
101000615613 Homo sapiens Mineralocorticoid receptor Proteins 0.000 description 1
101001109700 Homo sapiens Nuclear receptor subfamily 4 group A member 1 Proteins 0.000 description 1
CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
108020005497 Nuclear hormone receptor Proteins 0.000 description 1
102100022679 Nuclear receptor subfamily 4 group A member 1 Human genes 0.000 description 1
BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
101001023863 Rattus norvegicus Glucocorticoid receptor Proteins 0.000 description 1
101000906082 Rattus norvegicus Glutathione reductase Proteins 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
108010068068 Transcription Factor TFIIIA Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
108010090932 Vitellogenins Proteins 0.000 description 1
241000269370 Xenopus <genus> Species 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000012190 activator Substances 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
125000000539 amino acid group Chemical group 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
230000009118 appropriate response Effects 0.000 description 1
229960005261 aspartic acid Drugs 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
101150055766 cat gene Proteins 0.000 description 1
230000003915 cell function Effects 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
210000003483 chromatin Anatomy 0.000 description 1
210000001072 colon Anatomy 0.000 description 1
238000010276 construction Methods 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
238000011161 development Methods 0.000 description 1
238000009826 distribution Methods 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
229930182833 estradiol Natural products 0.000 description 1
239000000284 extract Substances 0.000 description 1
230000014509 gene expression Effects 0.000 description 1
102000054091 human NR3C2 Human genes 0.000 description 1
239000000411 inducer Substances 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
238000005304 joining Methods 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
244000005700 microbiome Species 0.000 description 1
239000002395 mineralocorticoid Substances 0.000 description 1
230000000869 mutational effect Effects 0.000 description 1
102000006255 nuclear receptors Human genes 0.000 description 1
108020004017 nuclear receptors Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
230000030648 nucleus localization Effects 0.000 description 1
230000037361 pathway Effects 0.000 description 1
229960003531 phenolsulfonphthalein Drugs 0.000 description 1
229960005190 phenylalanine Drugs 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000000186 progesterone Substances 0.000 description 1
229960003387 progesterone Drugs 0.000 description 1
230000004850 protein–protein interaction Effects 0.000 description 1
230000008844 regulatory mechanism Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
229930002330 retinoic acid Natural products 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000002904 solvent Substances 0.000 description 1
241000894007 species Species 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
230000037426 transcriptional repression Effects 0.000 description 1
230000010474 transient expression Effects 0.000 description 1
229960001727 tretinoin Drugs 0.000 description 1
230000001960 triggered effect Effects 0.000 description 1
QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 1
235000005282 vitamin D3 Nutrition 0.000 description 1
239000011647 vitamin D3 Substances 0.000 description 1
229940021056 vitamin d3 Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Immunology (AREA)
Biotechnology (AREA)
Endocrinology (AREA)
Medicinal Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Toxicology (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Analytical Chemistry (AREA)
General Engineering & Computer Science (AREA)
Physics & Mathematics (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Steroid Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

CA002047752A 1989-03-17 1990-03-16 Hormone response element compositions and assay Expired - Fee Related CA2047752C (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US32524089A	1989-03-17	1989-03-17
US325,240		1989-03-17
PCT/US1990/001428 WO1990011273A1 (en)	1989-03-17	1990-03-16	Hormone response element compositions and assay

Publications (2)

Publication Number	Publication Date
CA2047752A1 CA2047752A1 (en)	1990-09-18
CA2047752C true CA2047752C (en)	2001-07-10

Family

ID=23267026

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002047752A Expired - Fee Related CA2047752C (en)	1989-03-17	1990-03-16	Hormone response element compositions and assay

Country Status (8)

Country	Link
US (1)	US5597693A (de)
EP (1)	EP0463081B1 (de)
JP (1)	JPH04505012A (de)
AT (1)	ATE166360T1 (de)
AU (1)	AU655912B2 (de)
CA (1)	CA2047752C (de)
DE (1)	DE69032324T2 (de)
WO (1)	WO1990011273A1 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU665039B2 (en) *	1987-12-02	1995-12-14	Salk Institute For Biological Studies, The	Chimeric receptors and methods for identification
US5756448A (en) *	1992-02-26	1998-05-26	The General Hospital Corporation	Constitute activator of retinoid (CAR) receptor polypeptides
US6989242B1 (en)	1992-02-26	2006-01-24	The General Hospital Coporation	Car receptors and related molecules and methods
EP0577932A3 (de) *	1992-05-08	1995-04-19	American Cyanamid Co	Entwicklung von einem "Screening" für den Nachweis von androgenen Rezeptorenagonisten und Antagonisten.
US6242568B1 (en) *	1994-01-18	2001-06-05	The Scripps Research Institute	Zinc finger protein derivatives and methods therefor
US20050084885A1 (en) *	1994-01-18	2005-04-21	The Scripps Research Institute	Zinc finger protein derivatives and methods therefor
US6140466A (en) *	1994-01-18	2000-10-31	The Scripps Research Institute	Zinc finger protein derivatives and methods therefor
USRE39229E1 (en)	1994-08-20	2006-08-08	Gendaq Limited	Binding proteins for recognition of DNA
US6306649B1 (en) *	1995-06-27	2001-10-23	Ariad Gene Therapeutics, Inc.	Heterologous transcription factors
KR100263137B1 (ko) *	1995-09-08	2000-08-01	오테스코그 페르	오판 수용체
WO1997013864A1 (en)	1995-10-10	1997-04-17	Novartis Ag	Juvenile hormone or one of its agonists as a chemical ligand to control gene expression in plants by receptor mediated transactivation
US5728534A (en)	1996-07-19	1998-03-17	New England Medical Center Hospitals, Inc.	Methods for identifying cardiovascular therapeutic agents
US6692928B1 (en)	1996-07-19	2004-02-17	New England Medical Center Hospitals, Inc.	Methods for identifying cardiovascular agents
GB9710809D0 (en)	1997-05-23	1997-07-23	Medical Res Council	Nucleic acid binding proteins
US5972639A (en) *	1997-07-24	1999-10-26	Irori	Fluorescence-based assays for measuring cell proliferation
US6479653B1 (en) *	1997-08-26	2002-11-12	Ariad Gene Therapeutics, Inc.	Compositions and method for regulation of transcription
GB9718455D0 (en) *	1997-09-02	1997-11-05	Mcgregor Duncan P	Chimeric binding peptide library screening method
US6222015B1 (en)	1997-09-08	2001-04-24	Merck & Co., Inc.	Estrogen receptor
US6410248B1 (en)	1998-01-30	2002-06-25	Massachusetts Institute Of Technology	General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
ES2341926T3 (es) *	1998-03-02	2010-06-29	Massachusetts Institute Of Technology	Poliproteinas con dedos de cinc que tienen enlazadores mejorados.
CA2323064C (en)	1998-03-17	2011-05-31	Gendaq Limited	Nucleic acid binding proteins
CA2336207C (en)	1998-09-10	2004-03-23	Pioneer Hi-Bred International, Inc.	Ecdysone receptors and methods for their use
US6140081A (en) *	1998-10-16	2000-10-31	The Scripps Research Institute	Zinc finger binding domains for GNN
DE19855013C2 (de)	1998-11-20	2001-09-13	Schering Ag	Androgen- und Progesteron-Rezeptor bindendes Hormon Response Element
US8713641B1 (en)	1998-12-08	2014-04-29	Nomadix, Inc.	Systems and methods for authorizing, authenticating and accounting users having transparent computer access to a network using a gateway device
US8266266B2 (en) *	1998-12-08	2012-09-11	Nomadix, Inc.	Systems and methods for providing dynamic network authorization, authentication and accounting
US7013219B2 (en) *	1999-01-12	2006-03-14	Sangamo Biosciences, Inc.	Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en)	1999-01-12	2006-07-04	Sangamo Biosciences, Inc.	Ligand-controlled regulation of endogenous gene expression
US6453242B1 (en) *	1999-01-12	2002-09-17	Sangamo Biosciences, Inc.	Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en)	1999-01-12	2003-03-18	Sangamo Biosciences, Inc.	Regulation of endogenous gene expression in cells using zinc finger proteins
US6599692B1 (en)	1999-09-14	2003-07-29	Sangamo Bioscience, Inc.	Functional genomics using zinc finger proteins
DE19907099A1 (de) *	1999-02-19	2000-09-07	Theragene Biomedical Lab Gmbh	Hormon-Hormonrezeptorkomplexe und Nukleinsäurekonstrukte und ihre Verwendung in der Gentherapie
US20030104526A1 (en)	1999-03-24	2003-06-05	Qiang Liu	Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en)	2000-11-20	2004-09-21	Sangamo Biosciences, Inc.	Iterative optimization in the design of binding proteins
US7030215B2 (en) *	1999-03-24	2006-04-18	Sangamo Biosciences, Inc.	Position dependent recognition of GNN nucleotide triplets by zinc fingers
US20020132223A1 (en) *	1999-03-26	2002-09-19	City Of Hope	Methods for modulating activity of the FXR nuclear receptor
US6548739B2 (en)	1999-06-18	2003-04-15	City Of Hope	Method for activating peroxisome proliferator activated receptor-γ
US6586189B2 (en)	1999-06-18	2003-07-01	City Of Hope	Screening method for PPAR-γ ligands
AU776576B2 (en) *	1999-12-06	2004-09-16	Sangamo Biosciences, Inc.	Methods of using randomized libraries of zinc finger proteins for the identification of gene function
ATE355368T1 (de) *	2000-01-24	2006-03-15	Gendaq Ltd	Nucleinsäure bindende polypeptide gekennzeichnet durch flexible linker verbundene nucleinsäuredomäne
AU2001252944A1 (en) *	2000-03-24	2001-10-08	City Of Hope	Methods of modulating drug clearance mechanisms by altering SXR activity
EP1356066A2 (de)	2000-10-24	2003-10-29	Syngenta Participations AG	Kontrolle der pflanzengenexpression
US7067317B2 (en) *	2000-12-07	2006-06-27	Sangamo Biosciences, Inc.	Regulation of angiogenesis with zinc finger proteins
AU2002228841C1 (en) *	2000-12-07	2006-11-23	Sangamo Biosciences, Inc	Regulation of angiogenesis with zinc finger proteins
WO2002057293A2 (en)	2001-01-22	2002-07-25	Sangamo Biosciences, Inc.	Modified zinc finger binding proteins
US7067617B2 (en) *	2001-02-21	2006-06-27	The Scripps Research Institute	Zinc finger binding domains for nucleotide sequence ANN
AU2002333682A1 (en) *	2001-08-02	2003-02-17	Gencell S.A.	Inducible expression systems employing ppar transcriptional activators
EP1288303A1 (de) *	2001-08-23	2003-03-05	Aventis Pharma S.A.	Induzierbare Expressionssysteme unter Verwendung von PPAR Transkriptionsaktivatoren
US7262054B2 (en) *	2002-01-22	2007-08-28	Sangamo Biosciences, Inc.	Zinc finger proteins for DNA binding and gene regulation in plants
US7361635B2 (en)	2002-08-29	2008-04-22	Sangamo Biosciences, Inc.	Simultaneous modulation of multiple genes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4981784A (en) *	1987-12-02	1991-01-01	The Salk Institute For Biological Studies	Retinoic acid receptor method
US5171671A (en) *	1987-12-02	1992-12-15	The Salk Institute For Biological Studies	Retinoic acid receptor composition
US5274077A (en) *	1987-12-02	1993-12-28	The Salk Institute For Biological Studies	Retinoic acid receptor composition

1990
- 1990-03-16 AT AT90905299T patent/ATE166360T1/de not_active IP Right Cessation
- 1990-03-16 DE DE69032324T patent/DE69032324T2/de not_active Expired - Fee Related
- 1990-03-16 CA CA002047752A patent/CA2047752C/en not_active Expired - Fee Related
- 1990-03-16 JP JP2505257A patent/JPH04505012A/ja active Pending
- 1990-03-16 US US07/494,618 patent/US5597693A/en not_active Expired - Lifetime
- 1990-03-16 AU AU53423/90A patent/AU655912B2/en not_active Ceased
- 1990-03-16 WO PCT/US1990/001428 patent/WO1990011273A1/en not_active Ceased
- 1990-03-16 EP EP90905299A patent/EP0463081B1/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0463081A4 (en)	1992-08-12
JPH04505012A (ja)	1992-09-03
EP0463081B1 (de)	1998-05-20
EP0463081A1 (de)	1992-01-02
CA2047752A1 (en)	1990-09-18
AU655912B2 (en)	1995-01-19
DE69032324T2 (de)	1998-10-08
AU5342390A (en)	1990-10-22
WO1990011273A1 (en)	1990-10-04
ATE166360T1 (de)	1998-06-15
DE69032324D1 (de)	1998-06-25
US5597693A (en)	1997-01-28

Legal Events

Date	Code	Title	Description
1996-12-23	EEER	Examination request
2003-03-17	MKLA	Lapsed

Publication	Publication Date	Title
CA2047752C (en)	2001-07-10	Hormone response element compositions and assay
Willy et al.	1995	LXR, a nuclear receptor that defines a distinct retinoid response pathway.
EP0325849B1 (de)	1995-07-05	Retinoesäure-Rezeptor-Komposition und Verfahren zur Ligand-Identifizierung
US5171671A (en)	1992-12-15	Retinoic acid receptor composition
Crabtree et al.	1982	Organization of the rat γ-fibrinogen gene: alternative mRNA splice patterns produce the γA and γB (γ′) chains of fibrinogen
Umesono et al.	1989	Determinants of target gene specificity for steroid/thyroid hormone receptors
Kakizuka et al.	1991	Chromosomal translocation t (15; 17) in human acute promyelocytic leukemia fuses RARα with a novel putative transcription factor, PML
Forman et al.	1990	Minireview: interactions among a subfamily of nuclear hormone receptors: the regulatory zipper model
US7033828B2 (en)	2006-04-25	Nucleic acid encoding gamma retinoic acid receptor fusion protein
CA2210248A1 (en)	1996-07-18	Allosteric control of nuclear hormone receptors
WO1996021726A1 (en)	1996-07-18	Identification of a distinct retinoid-responsive pathway and uses therefor
Wisdom et al.	1992	Transformation by FosB requires a trans-activation domain missing in FosB2 that can be substituted by heterologous activation domains.
WO1996021726A9 (en)	1996-09-19	Identification of a distinct retinoid-responsive pathway and uses therefor
AU639699B2 (en)	1993-08-05	Dominant negative members of the steroid/thyroid superfamily of receptors
Hermann et al.	1991	Regulatory functions of a non-ligand-binding thyroid hormone receptor isoform.
US5274077A (en)	1993-12-28	Retinoic acid receptor composition
US5548063A (en)	1996-08-20	Retinoic acid receptor alpha proteins
AU665039B2 (en)	1995-12-14	Chimeric receptors and methods for identification
CA1341422C (en)	2003-02-25	Retinoic acid receptor composition and method
GB2265376A (en)	1993-09-29	Human steroid hormone receptor (nuci)