CA2055302A1 - Methode de detection de certaines souches microbiennes - Google Patents

Methode de detection de certaines souches microbiennes

Info

Publication number: CA2055302A1
Authority: CA; Canada
Prior art keywords: primer; sequence; dna; transposable element; primers
Prior art date: 1990-11-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2055302

Other languages

English (en)

Inventor

Roger G. L. Wheatcroft

R. Campbell Wyndham

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Canada, AS REPRESENTED BY MINISTE R OF AGRICULTURE CANADA

Carleton University

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-11-15

Filing date

1991-11-12

Publication date

1992-05-16

1991-11-12 Application filed by Individual filed Critical Individual

1992-05-16 Publication of CA2055302A1 publication Critical patent/CA2055302A1/fr

Status Abandoned legal-status Critical Current

Links

238000001514 detection method Methods 0.000 title claims abstract description 18
230000000813 microbial effect Effects 0.000 title abstract description 7
238000000034 method Methods 0.000 claims abstract description 43
108020004414 DNA Proteins 0.000 claims abstract 12
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 9
238000003780 insertion Methods 0.000 claims description 26
230000037431 insertion Effects 0.000 claims description 26
239000000523 sample Substances 0.000 claims description 17
239000012634 fragment Substances 0.000 claims description 15
244000005700 microbiome Species 0.000 claims description 14
239000002773 nucleotide Substances 0.000 claims description 13
125000003729 nucleotide group Chemical group 0.000 claims description 13
241000589196 Sinorhizobium meliloti Species 0.000 claims description 10
238000009396 hybridization Methods 0.000 claims description 10
230000001580 bacterial effect Effects 0.000 claims description 9
230000000295 complement effect Effects 0.000 claims description 7
230000015572 biosynthetic process Effects 0.000 claims description 5
238000001502 gel electrophoresis Methods 0.000 claims description 5
108020004707 nucleic acids Proteins 0.000 claims description 5
150000007523 nucleic acids Chemical class 0.000 claims description 5
102000039446 nucleic acids Human genes 0.000 claims description 5
238000003786 synthesis reaction Methods 0.000 claims description 5
241000894006 Bacteria Species 0.000 claims description 4
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
239000013599 cloning vector Substances 0.000 claims description 3
108091008146 restriction endonucleases Proteins 0.000 claims description 3
241000233866 Fungi Species 0.000 claims description 2
241000589187 Rhizobium sp. Species 0.000 claims 2
239000008241 heterogeneous mixture Substances 0.000 claims 1
238000010186 staining Methods 0.000 claims 1
230000001131 transforming effect Effects 0.000 claims 1
239000013615 primer Substances 0.000 abstract description 23
230000017105 transposition Effects 0.000 abstract description 9
108020001019 DNA Primers Proteins 0.000 abstract description 2
239000003155 DNA primer Substances 0.000 abstract description 2
238000003752 polymerase chain reaction Methods 0.000 description 12
150000002500 ions Chemical class 0.000 description 10
239000000499 gel Substances 0.000 description 9
238000000246 agarose gel electrophoresis Methods 0.000 description 4
230000002285 radioactive effect Effects 0.000 description 4
230000003321 amplification Effects 0.000 description 3
238000003199 nucleic acid amplification method Methods 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
239000004677 Nylon Substances 0.000 description 2
241000589180 Rhizobium Species 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
238000010367 cloning Methods 0.000 description 2
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238000002955 isolation Methods 0.000 description 2
235000021374 legumes Nutrition 0.000 description 2
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QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
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RRNIZKPFKNDSRS-UHFFFAOYSA-N Bensulide Chemical compound CC(C)OP(=S)(OC(C)C)SCCNS(=O)(=O)C1=CC=CC=C1 RRNIZKPFKNDSRS-UHFFFAOYSA-N 0.000 description 1
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QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
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102000003960 Ligases Human genes 0.000 description 1
108090000364 Ligases Proteins 0.000 description 1
240000004658 Medicago sativa Species 0.000 description 1
235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
241001267494 Microdes Species 0.000 description 1
241000186359 Mycobacterium Species 0.000 description 1
241000282320 Panthera leo Species 0.000 description 1
241000228143 Penicillium Species 0.000 description 1
241000221300 Puccinia Species 0.000 description 1
241000233639 Pythium Species 0.000 description 1
241000353161 Sakaguchia meli Species 0.000 description 1
241000605118 Thiobacillus Species 0.000 description 1
241000589634 Xanthomonas Species 0.000 description 1
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239000006166 lysate Substances 0.000 description 1
239000002068 microbial inoculum Substances 0.000 description 1
230000000877 morphologic effect Effects 0.000 description 1
238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
229920000523 polyvinylpolypyrrolidone Polymers 0.000 description 1
239000001253 polyvinylpolypyrrolidone Substances 0.000 description 1
235000013809 polyvinylpolypyrrolidone Nutrition 0.000 description 1
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239000011541 reaction mixture Substances 0.000 description 1
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IOVGROKTTNBUGK-SJCJKPOMSA-N ritodrine Chemical compound N([C@@H](C)[C@H](O)C=1C=CC(O)=CC=1)CCC1=CC=C(O)C=C1 IOVGROKTTNBUGK-SJCJKPOMSA-N 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
159000000000 sodium salts Chemical class 0.000 description 1
239000013598 vector Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA 2055302 1990-11-15 1991-11-12 Methode de detection de certaines souches microbiennes Abandoned CA2055302A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US613,061		1984-05-22
US61306190A	1990-11-15	1990-11-15

Publications (1)

Publication Number	Publication Date
CA2055302A1 true CA2055302A1 (fr)	1992-05-16

Family

ID=24455711

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2055302 Abandoned CA2055302A1 (fr)	1990-11-15	1991-11-12	Methode de detection de certaines souches microbiennes

Country Status (1)

Country	Link
CA (1)	CA2055302A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5656740A (en) *	1994-06-06	1997-08-12	E. I. Du Pont De Nemours And Company	Nucleic acid fragments useful in the detection of Salmonella
US5747257A (en) *	1996-02-29	1998-05-05	E. I. Du Pont De Nemours And Company	Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US5922538A (en) *	1996-11-08	1999-07-13	E.I. Du Pont De Nemours And Company	Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp

1991
- 1991-11-12 CA CA 2055302 patent/CA2055302A1/fr not_active Abandoned

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5656740A (en) *	1994-06-06	1997-08-12	E. I. Du Pont De Nemours And Company	Nucleic acid fragments useful in the detection of Salmonella
US5660981A (en) *	1994-06-06	1997-08-26	E. I. Du Pont De Nemours And Company	Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5747257A (en) *	1996-02-29	1998-05-05	E. I. Du Pont De Nemours And Company	Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US5922538A (en) *	1996-11-08	1999-07-13	E.I. Du Pont De Nemours And Company	Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp

Legal Events

Date	Code	Title	Description
1994-05-14	FZDE	Dead

Publication	Publication Date	Title
Kunkel et al.	1982	Regional localization on the human X of DNA segments cloned from flow sorted chromosomes
Watson et al.	1987	Developmental regulation of cytosine methylation in the nuclear ribosomal RNA genes of Pisum sativum
AU716675B2 (en)	2000-03-02	DNA-sequence-based diagnosis of mastitis from a milk sample
AU2656599A (en)	1999-08-23	Materials and methods for identifying and analyzing intermediate tandem repeat dna markers
EP0199439B1 (fr)	1993-09-08	Procédé et sonde pour détecter des cellules Actinobacillus Actinomycetemcomitans
US5795718A (en)	1998-08-18	Detection of complementary nucleotide sequences
EP0689591B1 (fr)	1997-08-06	Methode de contr le de l'invasion des graines de colza et autres cruciferes par le champignon leptosphaeria maculans
US6605439B2 (en)	2003-08-12	Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
AU712685B2 (en)	1999-11-11	RecA-assisted cloning of DNA
CA2086136A1 (fr)	1994-06-24	Sondes d'adn pour la detection de l'endomycorhize a arbuscules
EP0950719A2 (fr)	1999-10-20	Procédé de diagnose concernant la poursuite de tige à la consistence de caoutchouc utilisant un assay à la reaction de polymérase en châine
WO2010062897A1 (fr)	2010-06-03	Procédés et compositions pour détecter clostridium difficile
Robson	1986	Nitrogen fixation in strains of Azotobacter chroococcum bearing deletions of a cluster of genes coding for nitrogenase
CA2055302A1 (fr)	1992-05-16	Methode de detection de certaines souches microbiennes
EP0554437B1 (fr)	2000-04-05	Sondes d'acide nucleique utilisees dans la detection de shigella
WO1997044488A2 (fr)	1997-11-27	Compositions et procedes de detection de mycobacterium kansasii
WO2021026414A1 (fr)	2021-02-11	Enrichissement sélectif
AU769095B2 (en)	2004-01-15	Rapid and sensitive method for detecting (Histoplasma capsulatum)
CA2235630A1 (fr)	1998-03-12	Procedes et materiaux de detection de champignons
CA2082955A1 (fr)	1991-11-18	Methode de dosage d'agents mutagenes et materiel necessaire
EP0294595B1 (fr)	1996-01-24	Composition polynucléotidique et méthode pour sa production
AU731424B2 (en)	2001-03-29	Method for detecting resistant fungal cells in clinical material
KR101855955B1 (ko)	2018-05-11	벼 이삭마름병 진단용 마커 조성물 및 이를 포함하는 키트
KR20010032036A (ko)	2001-04-16	특이적 뉴클레오타이드 서열의 검출방법 및 조성물
JP2001178498A (ja)	2001-07-03	１６ＳｒＲＮＡ遺伝子による放線菌の同定・検出及びモニタリング方法