CA2058346C - Appareil et methode servant a l'electrophorese automatique - Google Patents

Appareil et methode servant a l'electrophorese automatique

Info

Publication number: CA2058346C
Authority: CA; Canada
Prior art keywords: electrophoresis; support medium; anode; plate; cathode
Prior art date: 1991-01-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA 2058346

Other languages

English (en)

Other versions

CA2058346A1 (fr

Inventor

Robert J. Sarrine

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Helena Laboratories Corp

Original Assignee

Helena Laboratories Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-01-16

Filing date

1991-12-23

Publication date

1999-03-30

1991-01-16 Priority claimed from US07/642,119 external-priority patent/US5234911A/en

1991-12-23 Application filed by Helena Laboratories Corp filed Critical Helena Laboratories Corp

1992-07-17 Publication of CA2058346A1 publication Critical patent/CA2058346A1/fr

1999-03-30 Application granted granted Critical

1999-03-30 Publication of CA2058346C publication Critical patent/CA2058346C/fr

2011-12-23 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

238000001962 electrophoresis Methods 0.000 title claims abstract description 143
238000000034 method Methods 0.000 title description 44
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 38
230000005291 magnetic effect Effects 0.000 claims description 32
229910052742 iron Inorganic materials 0.000 claims description 19
239000000463 material Substances 0.000 claims description 16
239000004020 conductor Substances 0.000 claims description 5
230000005611 electricity Effects 0.000 claims description 4
CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 10
230000006870 function Effects 0.000 abstract description 15
238000012545 processing Methods 0.000 abstract description 8
239000000523 sample Substances 0.000 description 93
239000003153 chemical reaction reagent Substances 0.000 description 35
239000007788 liquid Substances 0.000 description 34
238000001035 drying Methods 0.000 description 31
230000008569 process Effects 0.000 description 25
238000011534 incubation Methods 0.000 description 20
238000001816 cooling Methods 0.000 description 18
239000012530 fluid Substances 0.000 description 14
238000010186 staining Methods 0.000 description 11
239000011159 matrix material Substances 0.000 description 9
230000007480 spreading Effects 0.000 description 9
238000003892 spreading Methods 0.000 description 9
230000005684 electric field Effects 0.000 description 8
239000000499 gel Substances 0.000 description 8
239000012128 staining reagent Substances 0.000 description 8
239000000126 substance Substances 0.000 description 8
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
230000003287 optical effect Effects 0.000 description 7
238000000926 separation method Methods 0.000 description 7
229920000936 Agarose Polymers 0.000 description 6
238000010276 construction Methods 0.000 description 6
238000010438 heat treatment Methods 0.000 description 6
238000004458 analytical method Methods 0.000 description 5
229910002804 graphite Inorganic materials 0.000 description 5
239000010439 graphite Substances 0.000 description 5
150000002500 ions Chemical class 0.000 description 5
230000000903 blocking effect Effects 0.000 description 4
238000010217 densitometric analysis Methods 0.000 description 4
238000010586 diagram Methods 0.000 description 4
239000003302 ferromagnetic material Substances 0.000 description 4
238000007373 indentation Methods 0.000 description 4
238000012544 monitoring process Methods 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
238000012360 testing method Methods 0.000 description 4
238000000429 assembly Methods 0.000 description 3
230000000712 assembly Effects 0.000 description 3
230000008901 benefit Effects 0.000 description 3
239000000872 buffer Substances 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000012937 correction Methods 0.000 description 3
239000011521 glass Substances 0.000 description 3
230000005389 magnetism Effects 0.000 description 3
238000012546 transfer Methods 0.000 description 3
238000005406 washing Methods 0.000 description 3
238000012935 Averaging Methods 0.000 description 2
102000004506 Blood Proteins Human genes 0.000 description 2
108010017384 Blood Proteins Proteins 0.000 description 2
229920002799 BoPET Polymers 0.000 description 2
239000005041 Mylar™ Substances 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
229920002301 cellulose acetate Polymers 0.000 description 2
238000004140 cleaning Methods 0.000 description 2
238000004891 communication Methods 0.000 description 2
238000007865 diluting Methods 0.000 description 2
238000006073 displacement reaction Methods 0.000 description 2
238000011049 filling Methods 0.000 description 2
238000011065 in-situ storage Methods 0.000 description 2
238000009413 insulation Methods 0.000 description 2
239000000696 magnetic material Substances 0.000 description 2
239000000203 mixture Substances 0.000 description 2
238000011160 research Methods 0.000 description 2
210000002966 serum Anatomy 0.000 description 2
239000007787 solid Substances 0.000 description 2
239000000243 solution Substances 0.000 description 2
238000013519 translation Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
229920001817 Agar Polymers 0.000 description 1
241000282414 Homo sapiens Species 0.000 description 1
108010044467 Isoenzymes Proteins 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
YFONKFDEZLYQDH-OPQQBVKSSA-N N-[(1R,2S)-2,6-dimethyindan-1-yl]-6-[(1R)-1-fluoroethyl]-1,3,5-triazine-2,4-diamine Chemical compound C[C@@H](F)C1=NC(N)=NC(N[C@H]2C3=CC(C)=CC=C3C[C@@H]2C)=N1 YFONKFDEZLYQDH-OPQQBVKSSA-N 0.000 description 1
240000008881 Oenanthe javanica Species 0.000 description 1
241000282320 Panthera leo Species 0.000 description 1
229910000831 Steel Inorganic materials 0.000 description 1
230000009471 action Effects 0.000 description 1
230000009286 beneficial effect Effects 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
238000004590 computer program Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000000326 densiometry Methods 0.000 description 1
238000000151 deposition Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000009792 diffusion process Methods 0.000 description 1
239000012470 diluted sample Substances 0.000 description 1
230000000694 effects Effects 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000005286 illumination Methods 0.000 description 1
239000011810 insulating material Substances 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000007775 late Effects 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
238000005259 measurement Methods 0.000 description 1
238000011177 media preparation Methods 0.000 description 1
238000007431 microscopic evaluation Methods 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000000424 optical density measurement Methods 0.000 description 1
239000002245 particle Substances 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
238000005096 rolling process Methods 0.000 description 1
230000035939 shock Effects 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
239000010959 steel Substances 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Peptides Or Proteins (AREA)

CA 2058346 1991-01-16 1991-12-23 Appareil et methode servant a l'electrophorese automatique Expired - Fee Related CA2058346C (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US07/642,119 US5234911A (en)	1990-07-09	1991-01-16	Substance with interleukin-8 inhibiting activity and process for its preparation
US642,119		1991-01-16

Publications (2)

Publication Number	Publication Date
CA2058346A1 CA2058346A1 (fr)	1992-07-17
CA2058346C true CA2058346C (fr)	1999-03-30

Family

ID=24575288

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2058346 Expired - Fee Related CA2058346C (fr)	1991-01-16	1991-12-23	Appareil et methode servant a l'electrophorese automatique

Country Status (2)

Country	Link
CA (1)	CA2058346C (fr)
DE (1)	DE4200705A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ATE543093T1 (de) *	1998-09-10	2012-02-15	Wallac Oy	Analysegerät für ein grossflächiges bild

1991
- 1991-12-23 CA CA 2058346 patent/CA2058346C/fr not_active Expired - Fee Related
1992
- 1992-01-14 DE DE19924200705 patent/DE4200705A1/de not_active Withdrawn

Also Published As

Publication number	Publication date
DE4200705A1 (de)	1992-11-19
CA2058346A1 (fr)	1992-07-17

Legal Events

Date	Code	Title	Description
1992-06-08	EEER	Examination request
2010-12-23	MKLA	Lapsed

Publication	Publication Date	Title
US5147522A (en)	1992-09-15	Automatic electrophoresis apparatus and method
US4810348A (en)	1989-03-07	Automatic electrophoresis apparatus and method
US4890247A (en)	1989-12-26	Automatic electrophoresis apparatus and method
US4909920A (en)	1990-03-20	Automatic electrophoresis apparatus and method
US4986891A (en)	1991-01-22	Automatic electrophoresis apparatus and method
US4954237A (en)	1990-09-04	Automatic electrophoresis apparatus
EP0666980B1 (fr)	2002-05-02	Appareil d'electrophorese capillaire automatique
EP0687905B1 (fr)	2005-08-24	Appareil d'électrophorése capillaire automatique
US5963456A (en)	1999-10-05	Method and apparatus for displaying capillary electrophoresis data
US6017765A (en)	2000-01-25	Fluorescence detection capillary array electrophoresis analyzer
US20070138015A1 (en)	2007-06-21	Matrixes, Arrays, Systems and Methods
US7118659B2 (en)	2006-10-10	Robotic friendly external loading system for electrophoresis instrument and method
EP0397699B1 (fr)	1996-04-03	Appareil d'electrophorese capillaire automatique
JP2008249734A (ja)	2008-10-16	キャピラリアレイアッセンブリおよびキャピラリ電気泳動装置
US6068753A (en)	2000-05-30	Automatic electrophoresis apparatus with fluorescent and visible scanning
CA2058346C (fr)	1999-03-30	Appareil et methode servant a l'electrophorese automatique
US7261800B1 (en)	2007-08-28	Automatic in situ electrophoresis method and apparatus
JP2001165904A (ja)	2001-06-22	キャピラリ電気泳動装置およびキャピラリアレイアッセンブリ