CA2063575A1 - Methode servant a la production par recombinaison d'hirudines et de polypeptides de type hirudine - Google Patents

Methode servant a la production par recombinaison d'hirudines et de polypeptides de type hirudine

Info

Publication number
CA2063575A1
CA2063575A1 CA002063575A CA2063575A CA2063575A1 CA 2063575 A1 CA2063575 A1 CA 2063575A1 CA 002063575 A CA002063575 A CA 002063575A CA 2063575 A CA2063575 A CA 2063575A CA 2063575 A1 CA2063575 A1 CA 2063575A1
Authority
CA
Canada
Prior art keywords
hirudin
polypeptide
dna
host
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002063575A
Other languages
English (en)
Inventor
Luca Benatti
Paolo Carminati
Jacqueline Lansen
Guy Mazue
Romeo Roncucci
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2063575A1 publication Critical patent/CA2063575A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA002063575A 1990-05-10 1991-04-05 Methode servant a la production par recombinaison d'hirudines et de polypeptides de type hirudine Abandoned CA2063575A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909010552A GB9010552D0 (en) 1990-05-10 1990-05-10 Method for the recombinant production of hirudins and novel hirudins
GB9010552.9 1990-05-10

Publications (1)

Publication Number Publication Date
CA2063575A1 true CA2063575A1 (fr) 1991-11-11

Family

ID=10675801

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002063575A Abandoned CA2063575A1 (fr) 1990-05-10 1991-04-05 Methode servant a la production par recombinaison d'hirudines et de polypeptides de type hirudine

Country Status (16)

Country Link
EP (1) EP0482144A1 (fr)
JP (1) JPH05500615A (fr)
KR (1) KR920703817A (fr)
CN (1) CN1057294A (fr)
AU (1) AU7652591A (fr)
CA (1) CA2063575A1 (fr)
CS (1) CS133191A3 (fr)
FI (1) FI920058A0 (fr)
GB (1) GB9010552D0 (fr)
HU (1) HUT59961A (fr)
IE (1) IE911578A1 (fr)
IL (1) IL98074A0 (fr)
NO (1) NO920116L (fr)
PT (1) PT97604A (fr)
WO (1) WO1991017250A1 (fr)
ZA (1) ZA913503B (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2129749T3 (es) * 1990-11-08 1999-06-16 Japan Energy Corp Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo.
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
US5972648A (en) * 1993-09-28 1999-10-26 Japan Energy Corporation Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients
DE102005002978B4 (de) * 2005-01-21 2013-04-25 Merz Pharma Gmbh & Co. Kgaa Rekombinante Expression von Proteinen in einer disulfidverbrückten, zweikettigen Form
CN103402527B (zh) 2011-01-25 2016-04-20 鲁汶大学 用于细胞移植的组合物和方法
JP6034406B2 (ja) 2012-01-25 2016-11-30 ユニヴェルシテ カソリック ド ルーヴァンＵｎｉｖｅｒｓｉｔｅ Ｃａｔｈｏｌｉｑｕｅ Ｄｅ Ｌｏｕｖａｉｎ 細胞移植のための組成物および方法
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法
CN115677850B (zh) * 2021-07-24 2024-03-08 王大勇 具备较强抗凝血活性的医蛭基因突变水蛭素及其制备方法
CN114736289B (zh) * 2022-03-17 2023-07-18 华南理工大学 一种带有酪氨酸硫酸化修饰的水蛭素的化学合成方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins

Also Published As

Publication number Publication date
NO920116D0 (no) 1992-01-09
WO1991017250A1 (fr) 1991-11-14
AU7652591A (en) 1991-11-27
NO920116L (no) 1992-03-10
PT97604A (pt) 1992-02-28
CS133191A3 (en) 1992-01-15
FI920058A7 (fi) 1992-01-07
FI920058A0 (fi) 1992-01-07
HU9200088D0 (en) 1992-06-29
CN1057294A (zh) 1991-12-25
EP0482144A1 (fr) 1992-04-29
JPH05500615A (ja) 1993-02-12
KR920703817A (ko) 1992-12-18
IE911578A1 (en) 1991-11-20
ZA913503B (en) 1992-02-26
HUT59961A (en) 1992-07-28
GB9010552D0 (en) 1990-07-04
IL98074A0 (en) 1992-06-21

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Legal Events

Date Code Title Description
FZDE Discontinued