CA2080378A1 - Hollow fiber cell propagation - Google Patents

Hollow fiber cell propagation

Info

Publication number: CA2080378A1
Authority: CA; Canada
Prior art keywords: bioreactors; hollow fiber; loop; fluids; basal medium
Prior art date: 1991-02-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002080378A

Other languages

English (en)

French (fr)

Inventor

Timothy C. Gebhard

Uday K. Veeramallu

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

UNISYN TECHNOLOGIES Corp

Original Assignee

UNISYN TECHNOLOGIES Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-02-12

Filing date

1991-03-01

Publication date

1992-08-13

1991-03-01 Application filed by UNISYN TECHNOLOGIES Corp filed Critical UNISYN TECHNOLOGIES Corp

1992-08-13 Publication of CA2080378A1 publication Critical patent/CA2080378A1/en

Status Abandoned legal-status Critical Current

Links

239000012510 hollow fiber Substances 0.000 title claims abstract description 106
239000007640 basal medium Substances 0.000 claims abstract description 100
238000000034 method Methods 0.000 claims abstract description 80
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 71
239000001301 oxygen Substances 0.000 claims abstract description 70
229910052760 oxygen Inorganic materials 0.000 claims abstract description 70
239000000203 mixture Substances 0.000 claims abstract description 45
239000002699 waste material Substances 0.000 claims abstract description 25
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WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 74
239000008103 glucose Substances 0.000 claims description 74
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IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 17
238000011144 upstream manufacturing Methods 0.000 claims description 14
239000012528 membrane Substances 0.000 claims description 13
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 12
230000001419 dependent effect Effects 0.000 claims description 12
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 11
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UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 30
229930182555 Penicillin Natural products 0.000 description 16
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 16
229940049954 penicillin Drugs 0.000 description 16
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239000002054 inoculum Substances 0.000 description 13
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 12
229930182816 L-glutamine Natural products 0.000 description 12
239000012526 feed medium Substances 0.000 description 11
241001529936 Murinae Species 0.000 description 8
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239000012091 fetal bovine serum Substances 0.000 description 7
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JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 5
239000000463 material Substances 0.000 description 5
102000016611 Proteoglycans Human genes 0.000 description 4
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241000711484 Transmissible gastroenteritis virus Species 0.000 description 4
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WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
239000004417 polycarbonate Substances 0.000 description 4
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239000004809 Teflon Substances 0.000 description 2
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210000005056 cell body Anatomy 0.000 description 2
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RGCLLPNLLBQHPF-HJWRWDBZSA-N phosphamidon Chemical compound CCN(CC)C(=O)C(\Cl)=C(/C)OP(=O)(OC)OC RGCLLPNLLBQHPF-HJWRWDBZSA-N 0.000 description 2
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XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
241000699802 Cricetulus griseus Species 0.000 description 1
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101100194363 Schizosaccharomyces pombe (strain 972 / ATCC 24843) res2 gene Proteins 0.000 description 1
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150000001413 amino acids Chemical class 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
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239000007864 aqueous solution Substances 0.000 description 1
239000012888 bovine serum Substances 0.000 description 1
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238000007728 cost analysis Methods 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
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238000001537 electron coincidence spectroscopy Methods 0.000 description 1
239000003797 essential amino acid Substances 0.000 description 1
235000020776 essential amino acid Nutrition 0.000 description 1
230000001605 fetal effect Effects 0.000 description 1
239000006481 glucose medium Substances 0.000 description 1
230000007813 immunodeficiency Effects 0.000 description 1
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238000000338 in vitro Methods 0.000 description 1
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238000009413 insulation Methods 0.000 description 1
235000021048 nutrient requirements Nutrition 0.000 description 1
210000001672 ovary Anatomy 0.000 description 1
238000001139 pH measurement Methods 0.000 description 1
238000012856 packing Methods 0.000 description 1
239000002245 particle Substances 0.000 description 1
101150037117 pct-1 gene Proteins 0.000 description 1
239000012466 permeate Substances 0.000 description 1
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238000004382 potting Methods 0.000 description 1
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150000003839 salts Chemical class 0.000 description 1
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238000002798 spectrophotometry method Methods 0.000 description 1
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208000006379 syphilis Diseases 0.000 description 1
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239000011782 vitamin Substances 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/10—Hollow fibers or tubes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
General Engineering & Computer Science (AREA)
Sustainable Development (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
Thermal Sciences (AREA)
Physics & Mathematics (AREA)
Clinical Laboratory Science (AREA)
Immunology (AREA)
Computer Hardware Design (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
External Artificial Organs (AREA)
Materials For Medical Uses (AREA)

CA002080378A 1991-02-12 1991-03-01 Hollow fiber cell propagation Abandoned CA2080378A1 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US07/654,034 US5656421A (en)	1990-02-15	1991-02-12	Multi-bioreactor hollow fiber cell propagation system and method
US654,034		1991-02-12

Publications (1)

Publication Number	Publication Date
CA2080378A1 true CA2080378A1 (en)	1992-08-13

Family

ID=24623210

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002080378A Abandoned CA2080378A1 (en)	1991-02-12	1991-03-01	Hollow fiber cell propagation

Country Status (5)

Country	Link
US (1)	US5656421A (2)
EP (1)	EP0531297A1 (2)
AU (1)	AU658822B2 (2)
CA (1)	CA2080378A1 (2)
WO (1)	WO1992013940A1 (2)

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- 1991-03-01 AU AU74644/91A patent/AU658822B2/en not_active Ceased
- 1991-03-01 WO PCT/US1991/001386 patent/WO1992013940A1/en not_active Ceased

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Publication number	Publication date
US5656421A (en)	1997-08-12
AU658822B2 (en)	1995-05-04
WO1992013940A1 (en)	1992-08-20
EP0531297A4 (2)	1994-02-16
EP0531297A1 (en)	1993-03-17
AU7464491A (en)	1992-09-07

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Date	Code	Title	Description
1999-03-01	FZDE	Discontinued

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CA2080378A1 (en)	1992-08-13	Hollow fiber cell propagation
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CA1270455A (en)	1990-06-19	Apparatus and method for culturing cells, removing waste and concentrating product
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