CA2086535A1 - Installation d'electrophorese preparatoire - Google Patents
Installation d'electrophorese preparatoireInfo
- Publication number
- CA2086535A1 CA2086535A1 CA002086535A CA2086535A CA2086535A1 CA 2086535 A1 CA2086535 A1 CA 2086535A1 CA 002086535 A CA002086535 A CA 002086535A CA 2086535 A CA2086535 A CA 2086535A CA 2086535 A1 CA2086535 A1 CA 2086535A1
- Authority
- CA
- Canada
- Prior art keywords
- compartments
- capillaries
- cooling
- electrophoresis
- spacing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 34
- 238000001816 cooling Methods 0.000 claims abstract description 29
- 238000010276 construction Methods 0.000 claims abstract description 11
- 238000005192 partition Methods 0.000 claims description 15
- 239000003792 electrolyte Substances 0.000 claims description 10
- 239000012809 cooling fluid Substances 0.000 claims description 9
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 229920002401 polyacrylamide Polymers 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 3
- 239000011543 agarose gel Substances 0.000 claims description 2
- 229920006254 polymer film Polymers 0.000 claims description 2
- 229910000831 Steel Inorganic materials 0.000 claims 2
- 239000012811 non-conductive material Substances 0.000 claims 2
- 239000010959 steel Substances 0.000 claims 2
- 239000000499 gel Substances 0.000 description 23
- 238000000926 separation method Methods 0.000 description 19
- 239000000463 material Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 238000001155 isoelectric focusing Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 239000000498 cooling water Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 239000000959 ampholyte mixture Substances 0.000 description 6
- 239000004744 fabric Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 229920005862 polyol Polymers 0.000 description 6
- 150000003077 polyols Chemical class 0.000 description 6
- 239000002826 coolant Substances 0.000 description 5
- 239000008151 electrolyte solution Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 239000000110 cooling liquid Substances 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000012442 analytical experiment Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 230000007775 late Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000615 nonconductor Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Peptides Or Proteins (AREA)
- Exposure Of Semiconductors, Excluding Electron Or Ion Beam Exposure (AREA)
- Optical Elements Other Than Lenses (AREA)
- Exposure And Positioning Against Photoresist Photosensitive Materials (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19904021728 DE4021728A1 (de) | 1990-07-07 | 1990-07-07 | Vorrichtung fuer die praeparative elektrophorese |
| DEP4021728.0 | 1990-07-07 | ||
| PCT/EP1991/001219 WO1992000795A1 (fr) | 1990-07-07 | 1991-06-29 | Dispositif d'electrophorese preparatoire |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2086535A1 true CA2086535A1 (fr) | 1992-01-08 |
Family
ID=6409891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002086535A Abandoned CA2086535A1 (fr) | 1990-07-07 | 1991-06-29 | Installation d'electrophorese preparatoire |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0539399A1 (fr) |
| JP (1) | JPH05508577A (fr) |
| CA (1) | CA2086535A1 (fr) |
| DE (1) | DE4021728A1 (fr) |
| WO (1) | WO1992000795A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4116179A1 (de) * | 1991-05-17 | 1992-11-19 | Serva Feinbiochem Gmbh & Co | Elektrophoresekammer |
| US5522974A (en) * | 1993-10-21 | 1996-06-04 | Laurence A. Fishel | Method of preparing gel containing capillaries |
| DE19831210A1 (de) * | 1998-07-03 | 2000-01-05 | Wita Gmbh Wittmann Inst Of Tec | Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen |
| HK1049343A1 (zh) * | 1999-11-15 | 2003-05-09 | Proteome Systems Ltd. | 多間隔電泳方法及裝置 |
| AUPR051500A0 (en) | 2000-09-29 | 2000-10-26 | Proteome Systems Ltd | Electrophoresis system |
| US7622028B2 (en) | 2003-05-09 | 2009-11-24 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
| US7850835B2 (en) | 2003-05-09 | 2010-12-14 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3951777A (en) * | 1974-07-10 | 1976-04-20 | Hoffmann-La Roche Inc. | Isoelectric focusing devices |
| DE2746089A1 (de) * | 1977-10-13 | 1979-04-19 | Hahn Meitner Kernforsch | Vorrichtung zum trennen von in loesung vorliegenden ionen |
| DE3736087C2 (de) * | 1987-10-24 | 1997-03-06 | Serva Feinbiochem Gmbh & Co | Gewebe zur Herstellung von Elektrophoresegelen |
| DE3815758A1 (de) * | 1988-05-09 | 1990-03-01 | Serva Feinbiochem Gmbh & Co | Elektrophoresegele mit hohem polyolgehalt |
| GB8825957D0 (en) * | 1988-11-05 | 1988-12-14 | British Petroleum Co Plc | Mixing system |
| US5104505A (en) * | 1989-09-14 | 1992-04-14 | Separations Technology, Inc. | Method and apparatus for electrophoretic separations |
-
1990
- 1990-07-07 DE DE19904021728 patent/DE4021728A1/de not_active Ceased
-
1991
- 1991-06-29 EP EP19910911841 patent/EP0539399A1/fr not_active Ceased
- 1991-06-29 JP JP91510938A patent/JPH05508577A/ja active Pending
- 1991-06-29 WO PCT/EP1991/001219 patent/WO1992000795A1/fr not_active Ceased
- 1991-06-29 CA CA002086535A patent/CA2086535A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE4021728A1 (de) | 1992-01-09 |
| EP0539399A1 (fr) | 1993-05-05 |
| WO1992000795A1 (fr) | 1992-01-23 |
| JPH05508577A (ja) | 1993-12-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |