CA2088360A1 - Analyse quantitative et surveillance de la structure d'une proteine par chromatographie par soustraction - Google Patents

Analyse quantitative et surveillance de la structure d'une proteine par chromatographie par soustraction

Info

Publication number
CA2088360A1
CA2088360A1 CA002088360A CA2088360A CA2088360A1 CA 2088360 A1 CA2088360 A1 CA 2088360A1 CA 002088360 A CA002088360 A CA 002088360A CA 2088360 A CA2088360 A CA 2088360A CA 2088360 A1 CA2088360 A1 CA 2088360A1
Authority
CA
Canada
Prior art keywords
matrix
concentration
protein
sample
binding sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002088360A
Other languages
English (en)
Inventor
Noubar B. Afeyan
Fred E. Regnier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2088360A1 publication Critical patent/CA2088360A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/626—Detectors specially adapted therefor calibration, baseline
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
CA002088360A 1990-08-10 1991-08-01 Analyse quantitative et surveillance de la structure d'une proteine par chromatographie par soustraction Abandoned CA2088360A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56612190A 1990-08-10 1990-08-10
US566,121 1990-08-10
PCT/US1991/005544 WO1992002815A2 (fr) 1990-08-10 1991-08-01 Surveillance et analyse quantitative de la structure des proteines par chromatographie soustractive

Publications (1)

Publication Number Publication Date
CA2088360A1 true CA2088360A1 (fr) 1992-02-11

Family

ID=24261583

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002088360A Abandoned CA2088360A1 (fr) 1990-08-10 1991-08-01 Analyse quantitative et surveillance de la structure d'une proteine par chromatographie par soustraction

Country Status (5)

Country Link
EP (1) EP0548178A1 (fr)
JP (1) JPH06500396A (fr)
AU (1) AU654503B2 (fr)
CA (1) CA2088360A1 (fr)
WO (1) WO1992002815A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3175941B2 (ja) * 1991-03-28 2001-06-11 パーセプティブ バイオシステムズ インコーポレイテッド クロマトグラフ溶出液の減法に依るオンライン生成物確認方法及び装置
CA2240325A1 (fr) 1998-03-27 1998-11-14 Synsorb Biotech, Inc. Methodes de criblage de banques de constituants
US6720190B1 (en) 1998-03-27 2004-04-13 Ole Hindsgaul Methods for screening compound libraries
US6613575B1 (en) 1998-03-27 2003-09-02 Ole Hindsgaul Methods for screening compound libraries
US6054047A (en) 1998-03-27 2000-04-25 Synsorb Biotech, Inc. Apparatus for screening compound libraries
DE19945351C2 (de) * 1999-09-22 2002-04-18 Lohmann Therapie Syst Lts Verfahren zum Auffinden und zur Isolierung pharmakologisch wirksamer Verbindungen aus Substanzgemischen
CN102472731B (zh) * 2009-06-26 2016-01-13 通用电气健康护理生物科学股份公司 色谱系统中的方法
AU2019359263B2 (en) * 2018-10-09 2022-09-15 C Technologies, Inc. Mass control system for chromatography
CN114280208B (zh) * 2021-11-03 2024-05-24 鼎康(武汉)生物医药有限公司 一种亲和层析填料动态载量的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
JPS56114761A (en) * 1980-02-14 1981-09-09 Shinotesuto Kenkyusho:Kk Fractional determination method of zinc in fresh blood serum by affinity chromatography
EP0141259B1 (fr) * 1981-03-09 1989-06-07 Environmental Sciences Associates, Inc. Améliorations dans la chromatograhie liquide
JPS58216954A (ja) * 1982-06-11 1983-12-16 Shimadzu Corp 低温高速液体クロマトグラフイ
JPS5984828A (ja) * 1982-11-08 1984-05-16 Suntory Ltd 安定化免疫複合体およびその利用方法
IT1150598B (it) * 1982-12-14 1986-12-17 Ines Bianchi Procedimento per la purificazione della superossidodismutasi mediante cromatografia di affinita'
JPS59143958A (ja) * 1983-02-07 1984-08-17 Sekisui Chem Co Ltd フイブロネクチンの定量法
JPS63236964A (ja) * 1987-03-26 1988-10-03 Ube Ind Ltd 糖尿病の診断法
AU4800090A (en) * 1988-12-05 1990-06-26 Primus Corporation Method for determination of glycated proteinaceous species

Also Published As

Publication number Publication date
WO1992002815A2 (fr) 1992-02-20
WO1992002815A3 (fr) 1992-04-30
AU8530691A (en) 1992-03-02
AU654503B2 (en) 1994-11-10
JPH06500396A (ja) 1994-01-13
EP0548178A1 (fr) 1993-06-30

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued