CA2089495C - Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima - Google Patents

Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima Download PDF

Info

Publication number
CA2089495C
CA2089495C CA002089495A CA2089495A CA2089495C CA 2089495 C CA2089495 C CA 2089495C CA 002089495 A CA002089495 A CA 002089495A CA 2089495 A CA2089495 A CA 2089495A CA 2089495 C CA2089495 C CA 2089495C
Authority
CA
Canada
Prior art keywords
dna
dna polymerase
sequence
polymerise
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002089495A
Other languages
English (en)
Other versions
CA2089495A1 (fr
Inventor
David H. Gelfand
Frances C. Lawyer
Susanne Stoffel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/567,244 external-priority patent/US5374553A/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2089495A1 publication Critical patent/CA2089495A1/fr
Application granted granted Critical
Publication of CA2089495C publication Critical patent/CA2089495C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002089495A 1990-08-13 1991-08-13 Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima Expired - Lifetime CA2089495C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US567,244 1990-08-13
US07/567,244 US5374553A (en) 1986-08-22 1990-08-13 DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
PCT/US1991/005753 WO1992003556A1 (fr) 1990-08-13 1991-08-13 ENZYME D'ACIDE NUCLEIQUE THERMOSTABLE PURIFIEE PROVENANT DE L'EUBACTERIE $i(THERMOTOGA MARITIMA)

Publications (2)

Publication Number Publication Date
CA2089495A1 CA2089495A1 (fr) 1992-02-14
CA2089495C true CA2089495C (fr) 2007-04-03

Family

ID=37951652

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002089495A Expired - Lifetime CA2089495C (fr) 1990-08-13 1991-08-13 Enzyme acide nucleique polymerase thermostable et purifiee pour termotoga maritima

Country Status (1)

Country Link
CA (1) CA2089495C (fr)

Also Published As

Publication number Publication date
CA2089495A1 (fr) 1992-02-14

Similar Documents

Publication Publication Date Title
US5624833A (en) Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima
US5405774A (en) DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5455170A (en) Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
EP0550696B1 (fr) Enzyme de polymerase d'acide nucleique purifie thermostable derivee du thermosipho africanus
JP3227101B2 (ja) 熱安定性dnaポリメラーゼ
EP0506825B1 (fr) Vecteurs d'expression combines et procedes de purification de polymerase d'adn de thermus thermophilus
US5618711A (en) Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5466591A (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5795762A (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
CA2071213C (fr) Transcriptases inverses de haute temperature
NZ221517A (en) Thermostable enzyme: thermus aquaticus dna polymerase, and processes for amplifying, detecting and/or cloning nucleic acid sequences using said enzyme
EP2208789A1 (fr) Polymérases ADN dotées de propriétées d'incorporation de nucléotide étiquetée améliorée
CA1337405C (fr) Purification d'un enzyme thermostable

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry