CA2090701A1 - Solubilisation de proteines sous des formes actives - Google Patents

Solubilisation de proteines sous des formes actives

Info

Publication number
CA2090701A1
CA2090701A1 CA2090701A CA2090701A CA2090701A1 CA 2090701 A1 CA2090701 A1 CA 2090701A1 CA 2090701 A CA2090701 A CA 2090701A CA 2090701 A CA2090701 A CA 2090701A CA 2090701 A1 CA2090701 A1 CA 2090701A1
Authority
CA
Canada
Prior art keywords
protein
solubilization
proteins
active forms
refolding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2090701A
Other languages
English (en)
Other versions
CA2090701C (fr
Inventor
Enzo Crivelli
Nirdosh K. Puri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NATINCO NV
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2090701A1 publication Critical patent/CA2090701A1/fr
Application granted granted Critical
Publication of CA2090701C publication Critical patent/CA2090701C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé de préparation d'une protéine sous une forme physiologiquement active, ledit procédé comprenant l'utilisation d'une source de protéine sous une forme au moins partiellement solubilisée; et d'un agent de réplication comprenant une quantité efficace d'un monothiol de faible masse moléculaire ou d'un dérivé de ce dernier. Dans ce procédé, on soumet la protéine solubilisée à une étape de réplication, en présence de l'agent de réplication.
CA002090701A 1990-09-05 1991-08-29 Solubilisation de proteines sous des formes actives Expired - Fee Related CA2090701C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPK2159 1990-09-05
AUPK215990 1990-09-05
PCT/AU1991/000395 WO1992004382A1 (fr) 1990-09-05 1991-08-29 Solubilisation de proteines sous des formes actives

Publications (2)

Publication Number Publication Date
CA2090701A1 true CA2090701A1 (fr) 1992-03-10
CA2090701C CA2090701C (fr) 2000-05-16

Family

ID=3774938

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002090701A Expired - Fee Related CA2090701C (fr) 1990-09-05 1991-08-29 Solubilisation de proteines sous des formes actives

Country Status (8)

Country Link
EP (1) EP0547102B1 (fr)
AT (1) ATE168115T1 (fr)
AU (1) AU664021B2 (fr)
CA (1) CA2090701C (fr)
DE (1) DE69129747T2 (fr)
DK (1) DK0547102T3 (fr)
ES (1) ES2119779T3 (fr)
WO (1) WO1992004382A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4665193A (en) * 1992-07-02 1994-01-31 Pitman-Moore, Inc. A process for recovering a recombinant protein, in biologically active form, from a solution containing inactive protein
DK44593D0 (da) * 1993-04-20 1993-04-20 Novo Nordisk As Fremgangsmaade til fremstilling af et polypeptid
WO1995008573A1 (fr) * 1993-09-22 1995-03-30 Ajinomoto Co., Inc. Peptide a action inhibant l'agregation plaquettaire et procede de production de ce compose
ZA955789B (en) * 1994-07-15 1996-03-11 Novo Nordisk As A method of converting a hydrophobic derivative of a polypeptide into the native form
DE10309691B4 (de) * 2003-02-28 2018-10-04 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Gewinnung von löslichen Proteinen aus der Biomasse rekombinanter Mikroorganismen
EA020621B1 (ru) 2009-06-22 2014-12-30 Амген Инк. Рефолдинг белков с использованием химически контролируемого окислительно-восстановительного состояния
US8940878B2 (en) 2009-06-25 2015-01-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
US10065987B2 (en) 2013-02-12 2018-09-04 Bristol-Myers Squibb Company High pH protein refolding methods
US10183967B2 (en) 2013-02-12 2019-01-22 Bristol-Myers Squibb Company Tangential flow filtration based protein refolding methods
EP2978770B1 (fr) 2013-03-29 2019-11-06 Dr. Reddy's Laboratories Limited Repliement de protéines
WO2017040363A1 (fr) * 2015-09-02 2017-03-09 Merck Sharp & Dohme Corp. Procédé d'obtention d'insuline avec liaisons disulfure correctement formées

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6079000A (ja) * 1983-10-04 1985-05-04 Takeda Chem Ind Ltd ヒトγ型インタ−フエロン単量体の製造法
DE3587526T3 (de) * 1984-12-06 2002-08-08 Amgen Inc.(N.D.Ges D. Staates Delaware), Thousand Oaks Serinproteaseinhibitoren und isolierungsverfahren dazu.
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
EP0295859B1 (fr) * 1987-06-15 1994-11-17 Southern Cross Biotech Pty.Ltd. Préparation de protéines dans les formes actives
KR890701759A (ko) * 1987-07-29 1989-12-21 스테이너 브이. 칸스태드 에스케리키아 콜라이내에서 발현된 사람 인터루킨-4의 정제방법
CA1340586C (fr) * 1988-09-23 1999-06-08 Cetus Corporation Methode pour recuperer l'interferon beta produit par voie microbienne
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3901719A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur herstellung eines insulinvorlaeufers
DE3901718A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur renaturierung falscher rekombinanten von insulinvorlaeufern
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen

Also Published As

Publication number Publication date
EP0547102A4 (fr) 1994-04-27
WO1992004382A1 (fr) 1992-03-19
CA2090701C (fr) 2000-05-16
ATE168115T1 (de) 1998-07-15
DE69129747T2 (de) 1998-11-12
AU8403591A (en) 1992-03-30
DE69129747D1 (de) 1998-08-13
ES2119779T3 (es) 1998-10-16
DK0547102T3 (da) 1999-04-19
AU664021B2 (en) 1995-11-02
EP0547102B1 (fr) 1998-07-08
EP0547102A1 (fr) 1993-06-23

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed