CA2093567A1 - Hybridation par soustraction - Google Patents

Hybridation par soustraction

Info

Publication number: CA2093567A1
Authority: CA; Canada
Prior art keywords: cdna; single stranded; rna; dna; mrna
Prior art date: 1993-04-07
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2093567

Other languages

English (en)

Inventor

Ian N. Hampson

Lynne Pope

John Butler

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

GE Healthcare Ltd

Original Assignee

Cancer Research Campaign Technology Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-04-07

Filing date

1993-04-07

Publication date

1994-10-08

1993-04-07 Application filed by Cancer Research Campaign Technology Ltd filed Critical Cancer Research Campaign Technology Ltd

1993-04-07 Priority to CA 2093567 priority Critical patent/CA2093567A1/fr

1994-10-08 Publication of CA2093567A1 publication Critical patent/CA2093567A1/fr

Status Abandoned legal-status Critical Current

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Computational Biology (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Immunology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA 2093567 1993-04-07 1993-04-07 Hybridation par soustraction Abandoned CA2093567A1 (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA 2093567 CA2093567A1 (fr)	1993-04-07	1993-04-07	Hybridation par soustraction

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA 2093567 CA2093567A1 (fr)	1993-04-07	1993-04-07	Hybridation par soustraction

Publications (1)

Publication Number	Publication Date
CA2093567A1 true CA2093567A1 (fr)	1994-10-08

Family

ID=4151428

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2093567 Abandoned CA2093567A1 (fr)	1993-04-07	1993-04-07	Hybridation par soustraction

Country Status (1)

Country	Link
CA (1)	CA2093567A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1998008973A1 (fr) *	1996-08-29	1998-03-05	Cancer Research Campaign Technology Limited	Amplification globale d'acides nucleiques
WO1999018236A1 (fr) *	1997-10-03	1999-04-15	The Victoria University Of Manchester	Obtention de sequences d'acide nucleique
US5958738A (en) *	1997-03-24	1999-09-28	Roche Diagnostics Corporation	Procedure for subtractive hybridization and difference analysis

1993
- 1993-04-07 CA CA 2093567 patent/CA2093567A1/fr not_active Abandoned

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1998008973A1 (fr) *	1996-08-29	1998-03-05	Cancer Research Campaign Technology Limited	Amplification globale d'acides nucleiques
US6066457A (en) *	1996-08-29	2000-05-23	Cancer Research Campaign Technology Limited	Global amplification of nucleic acids
US5958738A (en) *	1997-03-24	1999-09-28	Roche Diagnostics Corporation	Procedure for subtractive hybridization and difference analysis
US6235503B1 (en)	1997-03-24	2001-05-22	Roche Diagnostics Corporation	Procedure for subtractive hybridization and difference analysis
WO1999018236A1 (fr) *	1997-10-03	1999-04-15	The Victoria University Of Manchester	Obtention de sequences d'acide nucleique

Legal Events

Date	Code	Title	Description
2001-04-09	FZDE	Dead

Publication	Publication Date	Title
US5591575A (en)	1997-01-07	Subtraction hybridization employing aziridinylbenoquinone cross-linking agents
EP0327429B1 (fr)	1993-09-22	Marquage par ligation et restriction simultanée
US6506561B1 (en)	2003-01-14	Method of obtaining a library of tags capable of defining a specific state of a biological sample
US6203984B1 (en)	2001-03-20	Proportional amplification of mRNA from a linear template in vitro
KR102310441B1 (ko)	2021-10-07	Rna-염색질 상호작용 분석용 조성물 및 이의 용도
US5827658A (en)	1998-10-27	Isolation of amplified genes via cDNA subtractive hybridization
US6461814B1 (en)	2002-10-08	Method of identifying gene transcription patterns
WO2000070039A1 (fr)	2000-11-23	METHODE DE SOUSTRACTION D'ADNc PAR SUPPRESSION DE LA SYNTHESE D'ARNm CIBLES SPECIFIQUEMENT
US5604118A (en)	1997-02-18	Eukaryotic expression vector system
WO1997007244A1 (fr)	1997-02-27	SEPARATION DE GENES AMPLIFIES, PAR HYBRIDATION SOUSTRACTIVE D'ADNc
CA2535332A1 (fr)	2005-02-24	Procede d'identification rapide d'epissage alterne
JP2003533966A5 (fr)	2004-12-24
EP4047091A1 (fr)	2022-08-24	Procédé de détection d'acide nucléique cible, procédé de détection d'une molécule de liaison à un acide nucléique, et procédé d'évaluation de la capacité de liaison à un acide nucléique
Fuchs et al.	2000	Identification of differentially expressed genes by mutually subtracted RNA fingerprinting
JP3853161B2 (ja)	2006-12-06	微量ｍＲＮＡ及びｃＤＮＡの増幅方法
CA2093567A1 (fr)	1994-10-08	Hybridation par soustraction
US5702898A (en)	1997-12-30	Procedure for normalization of cDNA libraries
EP1854882A1 (fr)	2007-11-14	Procédé permettant d' obtenir une soustraction de polynucléotides
CN112266949A (zh)	2021-01-26	基于pcr反应的多核苷酸扩增方法
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