CA2124580C - Methode de selection de microorganismes recombinants ayant a leur surface au moins une molecule ayant une activite enzymatique - Google Patents

Methode de selection de microorganismes recombinants ayant a leur surface au moins une molecule ayant une activite enzymatique Download PDF

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Publication number: CA2124580C
Authority: CA; Canada
Prior art keywords: solid support; molecule; enzymatic activity; phages; arm
Prior art date: 1991-11-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002124580A

Other languages

English (en)

Other versions

CA2124580A1 (fr

Inventor

Jacques Fastrez

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Universite Catholique de Louvain UCL

Original Assignee

Universite Catholique de Louvain UCL

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-11-29

Filing date

1992-11-30

Publication date

2002-07-16

1992-11-30 Application filed by Universite Catholique de Louvain UCL filed Critical Universite Catholique de Louvain UCL

1993-06-10 Publication of CA2124580A1 publication Critical patent/CA2124580A1/fr

2002-07-16 Application granted granted Critical

2002-07-16 Publication of CA2124580C publication Critical patent/CA2124580C/fr

2012-11-30 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

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YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 1
230000004083 survival effect Effects 0.000 description 1
239000003440 toxic substance Substances 0.000 description 1
230000035899 viability Effects 0.000 description 1
210000002845 virion Anatomy 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
- C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10051—Methods of production or purification of viral material

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Analytical Chemistry (AREA)
Virology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

CA002124580A 1991-11-29 1992-11-30 Methode de selection de microorganismes recombinants ayant a leur surface au moins une molecule ayant une activite enzymatique Expired - Lifetime CA2124580C (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
BE9101106		1991-11-29
BE9101106A BE1006312A3 (fr)	1991-11-29	1991-11-29	Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique.
PCT/BE1992/000052 WO1993011242A1 (fr)	1991-11-29	1992-11-30	Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique

Publications (2)

Publication Number	Publication Date
CA2124580A1 CA2124580A1 (fr)	1993-06-10
CA2124580C true CA2124580C (fr)	2002-07-16

Family

ID=3885823

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002124580A Expired - Lifetime CA2124580C (fr)	1991-11-29	1992-11-30	Methode de selection de microorganismes recombinants ayant a leur surface au moins une molecule ayant une activite enzymatique

Country Status (16)

Country	Link
EP (1)	EP0617737B1 (fr)
JP (1)	JP4125364B2 (fr)
KR (1)	KR100243997B1 (fr)
AT (1)	ATE147783T1 (fr)
AU (1)	AU671089B2 (fr)
BE (1)	BE1006312A3 (fr)
BR (1)	BR9206970A (fr)
CA (1)	CA2124580C (fr)
DE (1)	DE69216853T2 (fr)
DK (1)	DK0617737T3 (fr)
ES (1)	ES2098555T3 (fr)
FI (1)	FI109131B (fr)
HU (1)	HU220876B1 (fr)
NO (1)	NO313593B1 (fr)
SG (1)	SG48094A1 (fr)
WO (1)	WO1993011242A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL104829A (en) *	1992-02-24	1999-04-11	Igen Inc	Selection is based on a response to the expression and concentration of catalytic units
AU778776B2 (en) *	1999-04-14	2004-12-23	Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The	High sensitivity phage display biomolecule detection

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5325030B2 (fr) *	1972-12-19	1978-07-24
US4165258A (en) *	1975-10-08	1979-08-21	University Of Pennsylvania	Plasminogen activating enzyme-specific competitive inhibitor
AU1043788A (en) *	1986-12-01	1988-06-30	Mcdonnell Douglas Corporation	Method of identifying unknown organisms
DE3833628A1 (de) *	1988-10-03	1990-04-12	Genlux Forschungsgesellschaft	Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
JP2944694B2 (ja) *	1988-10-04	1999-09-06	ディーエヌエープラントテクノロジーコーポレイション	検出可能な表現型のファージ形質導入による細菌の検出
JP2810138B2 (ja) *	1989-08-23	1998-10-15	昭和電工株式会社	酵素活性測定用充填剤、その使用方法および装置
ES2062394T3 (es) *	1989-09-29	1994-12-16	Rohm & Haas	Medio que contiene un ligando para la separacion cromatografica, procedimiento para la preparacion del medio y uso del medio para aislar moleculas naturales o sinteticas de una mezcla liquida.

1991
- 1991-11-29 BE BE9101106A patent/BE1006312A3/fr not_active IP Right Cessation
1992
- 1992-11-30 AU AU29373/92A patent/AU671089B2/en not_active Expired
- 1992-11-30 CA CA002124580A patent/CA2124580C/fr not_active Expired - Lifetime
- 1992-11-30 DE DE69216853T patent/DE69216853T2/de not_active Expired - Lifetime
- 1992-11-30 WO PCT/BE1992/000052 patent/WO1993011242A1/fr not_active Ceased
- 1992-11-30 EP EP92923617A patent/EP0617737B1/fr not_active Expired - Lifetime
- 1992-11-30 ES ES92923617T patent/ES2098555T3/es not_active Expired - Lifetime
- 1992-11-30 KR KR1019940701814A patent/KR100243997B1/ko not_active Expired - Fee Related
- 1992-11-30 AT AT92923617T patent/ATE147783T1/de active
- 1992-11-30 JP JP50965793A patent/JP4125364B2/ja not_active Expired - Lifetime
- 1992-11-30 DK DK92923617.2T patent/DK0617737T3/da active
- 1992-11-30 BR BR9206970A patent/BR9206970A/pt not_active IP Right Cessation
- 1992-11-30 HU HU9401593A patent/HU220876B1/hu unknown
- 1992-11-30 SG SG1996007002A patent/SG48094A1/en unknown
1994
- 1994-05-10 NO NO19941731A patent/NO313593B1/no not_active IP Right Cessation
- 1994-05-26 FI FI942469A patent/FI109131B/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
DE69216853D1 (de)	1997-02-27
JPH07505999A (ja)	1995-07-06
AU2937392A (en)	1993-06-28
EP0617737A1 (fr)	1994-10-05
BE1006312A3 (fr)	1994-07-19
DE69216853T2 (de)	1997-06-26
DK0617737T3 (da)	1997-07-07
NO313593B1 (no)	2002-10-28
EP0617737B1 (fr)	1997-01-15
HUT70310A (en)	1995-09-28
FI942469L (fi)	1994-05-26
HU220876B1 (en)	2002-06-29
WO1993011242A1 (fr)	1993-06-10
SG48094A1 (en)	1998-04-17
FI942469A0 (fi)	1994-05-26
ATE147783T1 (de)	1997-02-15
KR100243997B1 (ko)	2000-02-01
CA2124580A1 (fr)	1993-06-10
BR9206970A (pt)	1995-12-19
JP4125364B2 (ja)	2008-07-30
ES2098555T3 (es)	1997-05-01
HU9401593D0 (en)	1994-08-29
NO941731L (no)	1994-06-21
NO941731D0 (no)	1994-05-10
FI109131B (fi)	2002-05-31
AU671089B2 (en)	1996-08-15

Legal Events

Date	Code	Title	Description
1999-11-08	EEER	Examination request
2013-02-03	MKEX	Expiry	Effective date: 20121130

Publication	Publication Date	Title
US6831161B1 (en)	2004-12-14	Method of affinity separation and ligands for use therein
Sche et al.	1999	Display cloning: functional identification of natural product receptors using cDNA-phage display
US20030022178A1 (en)	2003-01-30	Identification of sortase gene
US20020081570A1 (en)	2002-06-27	System to detect protein-protein interactions
US20030153020A1 (en)	2003-08-14	Identification of sortase gene
Soumillion et al.	1994	Phage display of enzymes and in vitro selection for catalytic activity
Gaskin et al.	2001	Phage display combinatorial libraries of short peptides: ligand selection for protein purification
CA2383186A1 (fr)	2001-03-15	Technique d'identification de fonction de gene par l'utilisation de petites sondes moleculaires
CA2124580C (fr)	2002-07-16	Methode de selection de microorganismes recombinants ayant a leur surface au moins une molecule ayant une activite enzymatique
AU675783B2 (en)	1997-02-20	Reaction-based selection for expression of and concentrationof catalytic moieties
AU774776B2 (en)	2004-07-08	Proteome mining
US6642014B1 (en)	2003-11-04	Enzyme activity screen with direct substrate replacement
US6867010B1 (en)	2005-03-15	Enzyme activity screen with direct substrate reloading
Patwardhan et al.	1998	Phage-displayed libraries for the selection of optimal affinity peptides for protein purification using Ni-nitrilotriacetic Acid chromatography
Soumillion	2004	Selection of phage‐displayed enzymes
AU764664B2 (en)	2003-08-28	Enzyme activity screen with substrate replacement
EP1333096A1 (fr)	2003-08-06	Procedes pour identifier la fonction du gene par l'expression phagique
WO2003029821A1 (fr)	2003-04-10	Criblage iteratif assiste par cibles (tais), nouveau format de criblage pour grands repertoires moleculaires
Stolle	2005	Development of bispecific filamentous bacteriophages for the generation of a novel automated screening system based on phage display technology
Palombo	0	10 Phage selection for biopanning
Yang	1997	Analyzing structure function relationships of serine proteases and their macromolecular inhibitors by combinatorial biochemistry
HK1056895A (en)	2004-03-05	Method to identify gene function using phage display screening