CA2134539A1 - Procede de selection et/ou d'obtention de sondes capables de detecter de nouvelles regions a nombres variable de repetitions en tandem, sondes pouvant etre ainsi obtenues et leursutilisations - Google Patents

Procede de selection et/ou d'obtention de sondes capables de detecter de nouvelles regions a nombres variable de repetitions en tandem, sondes pouvant etre ainsi obtenues et leursutilisations

Info

Publication number
CA2134539A1
CA2134539A1 CA002134539A CA2134539A CA2134539A1 CA 2134539 A1 CA2134539 A1 CA 2134539A1 CA 002134539 A CA002134539 A CA 002134539A CA 2134539 A CA2134539 A CA 2134539A CA 2134539 A1 CA2134539 A1 CA 2134539A1
Authority
CA
Canada
Prior art keywords
probes
selecting
obtaining
detecting new
new
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002134539A
Other languages
English (en)
Other versions
CA2134539C (fr
Inventor
Jerome Buard
Dominique Gauguier
Gilles Vergnaud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gouvernement de la Republique Francaise
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2134539A1 publication Critical patent/CA2134539A1/fr
Application granted granted Critical
Publication of CA2134539C publication Critical patent/CA2134539C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention a pour objet un procédé de sélection et d'obtention de sondes nucléiques capables de détecter des nouvelles régions à nombre variable de répétitions en tandem (ou VNTR) présentes dans le génome des eucaryotes supérieurs. Sont également objet de l'invention les sondes ainsi obtenues ainsi que leur utilisations. Ce nouveau procédé permet d'obtenir aisément de nouvelles sondes capable de s'apparier à de nouvelles VNTR, en utilisant des fragments d'ADN génomique obtenus après hydrolyse d'ADN à
l'aide de certaines enzymes de restriction et sélectionnés d'après leur taille.
CA002134539A 1993-10-29 1994-10-27 Procede de selection et/ou d'obtention de sondes capables de detecter de nouvelles regions a nombres variable de repetitions en tandem, sondes pouvant etre ainsi obtenues et leursutilisations Expired - Fee Related CA2134539C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9312923 1993-10-29
FR9312923A FR2711672B1 (fr) 1993-10-29 1993-10-29 Procédé de sélection et/ou d'obtention de sondes capables de détecter de nouvelles régions à nombre variable de répétitions en tandem, sondes pouvant être ainsi obtenues et leurs utilisations.

Publications (2)

Publication Number Publication Date
CA2134539A1 true CA2134539A1 (fr) 1995-04-30
CA2134539C CA2134539C (fr) 2003-04-22

Family

ID=9452348

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002134539A Expired - Fee Related CA2134539C (fr) 1993-10-29 1994-10-27 Procede de selection et/ou d'obtention de sondes capables de detecter de nouvelles regions a nombres variable de repetitions en tandem, sondes pouvant etre ainsi obtenues et leursutilisations

Country Status (3)

Country Link
US (2) US5573912A (fr)
CA (1) CA2134539C (fr)
FR (1) FR2711672B1 (fr)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4963663A (en) * 1988-12-23 1990-10-16 University Of Utah Genetic identification employing DNA probes of variable number tandem repeat loci
EP0402400B1 (fr) * 1988-02-18 1999-09-08 University of Utah Identification genetique avec des adn d'investigation ayant des sites repetitifs en tandem en quantite variable
US5032501A (en) * 1989-02-10 1991-07-16 Virginia Mason Research Center DNA probes to vntr loci
US5075217A (en) * 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
US5097024A (en) * 1989-09-25 1992-03-17 Hodes Marion E DNA probes for fingerpint analysis without tandem repeats
US5273878A (en) * 1990-08-30 1993-12-28 John Groffen Nucleic acid probes that reveal hypervariable restriction fragment length polymorphisms within the ABR gene
US5364759B2 (en) * 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
US5427932A (en) * 1991-04-09 1995-06-27 Reagents Of The University Of California Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
US5378602A (en) * 1991-05-29 1995-01-03 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
FR2680520B1 (fr) * 1991-08-22 1995-09-22 France Etat Armement Procede de detection de nouvelles regions hypervariables dans une sequence d'adn, sequences de nucleotides constituant des sondes d'hybridation et leur application biologique.

Also Published As

Publication number Publication date
CA2134539C (fr) 2003-04-22
US5573912A (en) 1996-11-12
FR2711672A1 (fr) 1995-05-05
US5716786A (en) 1998-02-10
FR2711672B1 (fr) 1995-12-08

Similar Documents

Publication Publication Date Title
Mohaghegh et al. The Bloom’s and Werner’s syndrome proteins are DNA structure-specific helicases
Cantor et al. Genomics: the science and technology behind the human genome project
Puig Lombardi et al. Thermodynamically stable and genetically unstable G-quadruplexes are depleted in genomes across species
Vidal et al. Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step
US5994058A (en) Method for contiguous genome sequencing
Swerdlow et al. Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
Gregory et al. Genome mapping by fluorescent fingerprinting
Müller et al. Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution
DE69636080D1 (de) Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
FR2444713A1 (fr) Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant
EP0285123A3 (fr) Méthode de mutagénèse complète d'acides nucléiques
Tolun et al. A real‐time DNase assay (ReDA) based on PicoGreen® fluorescence
DE69133629D1 (de) nssystem zur Bestimmung von genetischen Krankheiten
Martín-Parras et al. Topological complexity of different populations of pBR322 as visualized by two-dimensional agarose gel electrophoresis
WO1998002575A1 (fr) Procede de sequençage genomique continu
US6083695A (en) Optimized primer library for gene sequencing and method of using same
Zain et al. Determination and analysis of adenovirus-2 DNA sequences which may include signals for late messenger RNA processing
Lim et al. Directed evolution of nucleotide-based libraries using lambda exonuclease
Zhang et al. Paired dCas9 design as a nucleic acid detection platform for pathogenic strains
Stewart et al. Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks
Emmerich et al. An imperfect inverted repeat is critical for DNA binding of the response regulator RegR of Bradyrhizobium japonicum
CA2134539A1 (fr) Procede de selection et/ou d'obtention de sondes capables de detecter de nouvelles regions a nombres variable de repetitions en tandem, sondes pouvant etre ainsi obtenues et leursutilisations
FR2680520B1 (fr) Procede de detection de nouvelles regions hypervariables dans une sequence d'adn, sequences de nucleotides constituant des sondes d'hybridation et leur application biologique.
Petree et al. MultiFRAGing: rapid and simultaneous genotyping of multiple alleles in a single reaction
Butorin et al. Comparison of the hydrolysis patterns of several tRNAs by cobra venom ribonuclease in different steps of the aminoacylation reaction

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20131029