CA2167501C - Methode pour l¨orientation directionnelle d¨embryons de plantes - Google Patents
Methode pour l¨orientation directionnelle d¨embryons de plantesInfo
- Publication number
- CA2167501C CA2167501C CA002167501A CA2167501A CA2167501C CA 2167501 C CA2167501 C CA 2167501C CA 002167501 A CA002167501 A CA 002167501A CA 2167501 A CA2167501 A CA 2167501A CA 2167501 C CA2167501 C CA 2167501C
- Authority
- CA
- Canada
- Prior art keywords
- embryos
- medium
- flotation
- flotation medium
- oriented
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000002257 embryonic structure Anatomy 0.000 title claims abstract description 151
- 238000000034 method Methods 0.000 title claims abstract description 46
- 238000005188 flotation Methods 0.000 claims abstract description 53
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 25
- 230000000392 somatic effect Effects 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 13
- 239000002609 medium Substances 0.000 claims description 56
- 239000012530 fluid Substances 0.000 claims description 20
- 239000006163 transport media Substances 0.000 claims description 18
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 231100001184 nonphytotoxic Toxicity 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 3
- 241000218633 Pinidae Species 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract description 15
- 241000218631 Coniferophyta Species 0.000 abstract description 10
- 238000003780 insertion Methods 0.000 abstract description 6
- 230000037431 insertion Effects 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 10
- 239000000499 gel Substances 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 8
- 230000035784 germination Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000002093 peripheral effect Effects 0.000 description 4
- 241000218679 Pinus taeda Species 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000036512 infertility Effects 0.000 description 3
- 210000002445 nipple Anatomy 0.000 description 3
- 240000007087 Apium graveolens Species 0.000 description 2
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 2
- 235000010591 Appio Nutrition 0.000 description 2
- 235000008566 Pinus taeda Nutrition 0.000 description 2
- 240000001416 Pseudotsuga menziesii Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000012736 aqueous medium Substances 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 235000014466 Douglas bleu Nutrition 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 235000005386 Pseudotsuga menziesii var menziesii Nutrition 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Inorganic materials [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- ZOMBKNNSYQHRCA-UHFFFAOYSA-J calcium sulfate hemihydrate Chemical compound O.[Ca+2].[Ca+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O ZOMBKNNSYQHRCA-UHFFFAOYSA-J 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical group C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000011507 gypsum plaster Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100000897 loss of orientation Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne la disposition d'embryons végétaux qui se caractérise en ce qu'une extrémité désignée de l'embryon soit toujours orientée dans un sens prédéterminé. Les embryons sont de préférence d'abord assez rapidement déshydratés jusqu'à ne présenter qu'une teneur en humidité de 15 % environ. Ils sont ensuite placés en suspension dans un liquide de flottation bénin ayant une masse volumique comprise entre environ 1059 et 1104 g/cm<3>. La masse volumique doit être ajustée de manière empirique de sorte qu'un nombre prédominant d'embryons viables flottent et d'embryons non viables se déposent au fond. Dans le cas au moins d'embryons somatiques de conifères, ceux-ci flottent, leur extrémité portant les cotylédons latents étant dirigée vers le haut. Après un temps de séparation suffisant dans le liquide de flottation, les embryons orientés sont balayés par un courant liquide et entraînés dans un conduit. Ils y entrent, les extrémités à cotylédons en premier, puis ils sont ensuite transportés vers un point d'évacuation sans perdre cette orientation, et ils sont séparés du liquide porteur. Les embryons, avec les extrémités à cotylédons toujours positionnées en avant, peuvent être ensuite récupérés par un robot ou autre dispositif afin de subir ensuite un traitement, tel que leur insertion dans une graine artificielle.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/865,390 US5284765A (en) | 1992-04-08 | 1992-04-08 | Method of directionally orienting plant embryos |
| PCT/US1993/007804 WO1995005064A1 (fr) | 1992-04-08 | 1993-08-18 | Procede d'orientation d'embryons vegetaux |
| BR9307878A BR9307878A (pt) | 1992-04-08 | 1993-08-18 | Processo para orientar direcionalmente uma multiplicidade dem embrioes de plantas |
| NZ255823A NZ255823A (en) | 1992-04-08 | 1993-08-18 | Method of orienting plant embryos such that a specified end is always oriented in a specific direction; separation of conifer somatic embryos |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2167501A1 CA2167501A1 (fr) | 1995-02-23 |
| CA2167501C true CA2167501C (fr) | 1999-12-07 |
Family
ID=29407851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002167501A Expired - Fee Related CA2167501C (fr) | 1992-04-08 | 1993-08-18 | Methode pour l¨orientation directionnelle d¨embryons de plantes |
Country Status (3)
| Country | Link |
|---|---|
| CA (1) | CA2167501C (fr) |
| NZ (1) | NZ255823A (fr) |
| WO (1) | WO1995005064A1 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6684564B1 (en) | 1999-08-23 | 2004-02-03 | Weyerhaeuser Company | Embryo delivery system for manufactured seeds |
| US7530197B2 (en) | 2003-06-30 | 2009-05-12 | Weyerhaeuser Co. | Automated system and method for harvesting and multi-stage screening of plant embryos |
| US7228658B2 (en) | 2003-08-27 | 2007-06-12 | Weyerhaeuser Company | Method of attaching an end seal to manufactured seeds |
| US7555865B2 (en) | 2003-11-25 | 2009-07-07 | Weyerhaeuser Nr Company | Method and system of manufacturing artificial seed coats |
| CA2486289C (fr) | 2003-11-25 | 2008-01-08 | Weyerhaeuser Company | Embout ferme et dispositif de blocage combines |
| CA2486311C (fr) | 2003-11-26 | 2008-08-12 | Weyerhaeuser Company | Dispositif de ramassage sous vide a degagement mecanique |
| US7356965B2 (en) | 2003-12-11 | 2008-04-15 | Weyerhaeuser Co. | Multi-embryo manufactured seed |
| US7591287B2 (en) | 2003-12-18 | 2009-09-22 | Weyerhaeuser Nr Company | System and method for filling a seedcoat with a liquid to a selected level |
| US7568309B2 (en) | 2004-06-30 | 2009-08-04 | Weyerhaeuser Nr Company | Method and system for producing manufactured seeds |
| US7547488B2 (en) | 2004-12-15 | 2009-06-16 | Weyerhaeuser Nr Company | Oriented strand board panel having improved strand alignment and a method for making the same |
| US7654037B2 (en) | 2005-06-30 | 2010-02-02 | Weyerhaeuser Nr Company | Method to improve plant somatic embryo germination from manufactured seed |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE442939B (sv) * | 1981-10-09 | 1986-02-10 | Milan Simak | Sett for sortering av fron |
| US4562663A (en) * | 1982-10-12 | 1986-01-07 | Plant Genetics, Inc. | Analogs of botanic seed |
| US4615141A (en) * | 1984-08-14 | 1986-10-07 | Purdue Research Foundation | Process for encapsulating asexual plant embryos |
| US5036007A (en) * | 1989-03-09 | 1991-07-30 | Weyerhaeuser Company | Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation |
| US4957866A (en) * | 1989-03-09 | 1990-09-18 | Weyerhaeuser Company | Method for reproducing coniferous plants by somatic embryogenesis |
| FI88119C (fi) * | 1989-07-06 | 1993-04-13 | Valtion Teknillinen | Foerfarande och apparatur foer plockning av smao partiklar ur vaetska |
| US5427593A (en) * | 1990-10-26 | 1995-06-27 | Weyerhaeuser Company | Analogs of botanic seed |
| US5284765A (en) * | 1992-04-08 | 1994-02-08 | Weyerhaeuser Company | Method of directionally orienting plant embryos |
-
1993
- 1993-08-18 CA CA002167501A patent/CA2167501C/fr not_active Expired - Fee Related
- 1993-08-18 WO PCT/US1993/007804 patent/WO1995005064A1/fr not_active Ceased
- 1993-08-18 NZ NZ255823A patent/NZ255823A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NZ255823A (en) | 1996-08-27 |
| CA2167501A1 (fr) | 1995-02-23 |
| WO1995005064A1 (fr) | 1995-02-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |